##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660765_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33781795 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.999762179599987 33.0 31.0 34.0 27.0 34.0 2 31.789950504406292 34.0 31.0 34.0 28.0 34.0 3 32.30116709310444 34.0 31.0 34.0 30.0 34.0 4 35.829622108594286 37.0 35.0 37.0 35.0 37.0 5 35.89020355490287 37.0 35.0 37.0 35.0 37.0 6 35.92961951844181 37.0 35.0 37.0 35.0 37.0 7 35.92544851450315 37.0 35.0 37.0 35.0 37.0 8 35.931757385893796 37.0 35.0 37.0 35.0 37.0 9 37.646854259816564 39.0 37.0 39.0 35.0 39.0 10-11 37.58806299369232 39.0 37.0 39.0 35.0 39.0 12-13 37.64879019898143 39.0 37.0 39.0 35.0 39.0 14-15 38.98023285914795 40.0 38.0 41.0 35.0 41.0 16-17 38.99482891598863 40.0 38.0 41.0 35.0 41.0 18-19 38.97993397035297 40.0 38.0 41.0 35.0 41.0 20-21 38.91784557333321 40.0 38.0 41.0 35.0 41.0 22-23 38.81683932129717 40.0 38.0 41.0 34.5 41.0 24-25 38.76227741894709 40.0 38.0 41.0 34.5 41.0 26-27 38.72304131559616 40.0 38.0 41.0 34.0 41.0 28-29 38.62266423379812 40.0 38.0 41.0 34.0 41.0 30-31 38.52836290966776 40.0 38.0 41.0 34.0 41.0 32-33 38.42677075034053 40.0 38.0 41.0 34.0 41.0 34-35 38.31982964493153 40.0 38.0 41.0 34.0 41.0 36-37 38.13997318674156 40.0 38.0 41.0 33.0 41.0 38-39 37.73685958665015 40.0 36.0 41.0 33.0 41.0 40-41 37.592043436413014 40.0 36.0 41.0 33.0 41.0 42-43 37.435718424672224 40.0 36.0 41.0 33.0 41.0 44-45 37.321770631193516 39.5 35.0 41.0 32.0 41.0 46-47 37.19171586352945 39.0 35.0 41.0 32.0 41.0 48-49 37.3154850563743 40.0 35.0 41.0 32.0 41.0 50-51 37.227233573586005 39.5 35.0 41.0 32.0 41.0 52-53 36.97626536718964 39.0 35.0 41.0 31.0 41.0 54-55 36.500701442892534 39.0 35.0 41.0 29.5 41.0 56-57 36.21997793486107 39.0 35.0 41.0 28.5 41.0 58-59 36.03148268172251 39.0 35.0 41.0 28.0 41.0 60-61 35.83841265687629 39.0 35.0 41.0 28.0 41.0 62-63 35.552397319325394 38.5 35.0 40.5 27.0 41.0 64-65 35.31192303428518 38.0 35.0 40.0 27.0 41.0 66-67 35.05604560089243 37.0 34.5 40.0 26.5 41.0 68-69 34.773709685941796 37.0 34.0 40.0 26.0 41.0 70-71 34.45145728638754 36.0 34.0 39.0 26.0 41.0 72-73 34.140238107536916 36.0 34.0 39.0 26.0 41.0 74-75 33.790013245299725 35.0 34.0 38.5 26.0 40.0 76-77 31.692288760854773 34.0 31.0 36.0 22.5 38.5 78-79 32.95778180229914 35.0 33.0 37.0 25.0 39.0 80-81 32.94178952006547 35.0 33.0 36.5 25.0 39.0 82-83 32.721251209416195 35.0 33.0 36.0 25.0 38.0 84-85 32.481803956835336 35.0 33.0 36.0 25.0 37.0 86-87 32.27075532842467 35.0 33.0 35.0 24.0 37.0 88-89 32.0491931082999 35.0 33.0 35.0 24.0 36.0 90-91 31.885992440602994 35.0 33.0 35.0 24.0 36.0 92-93 31.75708812986403 35.0 33.0 35.0 23.0 36.0 94-95 31.656259355075715 35.0 33.0 35.0 23.0 36.0 96-97 31.552180042534744 35.0 33.0 35.0 22.0 35.0 98-99 31.436437673012932 35.0 32.5 35.0 20.0 35.0 100 31.24850115276586 35.0 32.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7602781669407257 1101 2 1.2112157007265907 1101 3 0.7755003430937464 1101 4 0.6072409598822972 1101 5 0.4846936040309586 1101 6 0.425449968722738 1101 7 0.3877388855225803 1101 8 0.40789552133025353 1101 9 0.5075692958248865 1101 10-11 0.5044344381322006 1101 12-13 0.46481546765622994 1101 14-15 0.6322168960170984 1101 16-17 0.6145195140811524 1101 18-19 0.5534625331680871 1101 20-21 0.5784238701963176 1101 22-23 0.5649839122067846 1101 24-25 0.6149206651214101 1101 26-27 0.5952902973524345 1101 28-29 0.6058415902603471 1101 30-31 0.5796693226740999 1101 32-33 0.582064627195308 1101 34-35 0.5934443058226151 1101 36-37 0.5771421531324847 1101 38-39 0.682407038311645 1101 40-41 0.6554401470393572 1101 42-43 0.629717260203627 1101 44-45 0.6081794329125927 1101 46-47 0.5655009135670355 1101 48-49 0.5495474429947009 1101 50-51 0.5566991267951025 1101 52-53 0.5520351106155914 1101 54-55 0.6066612787661043 1101 56-57 0.6677311587779968 1101 58-59 0.672446755808501 1101 60-61 0.6143877382982694 1101 62-63 0.6887127761427152 1101 64-65 0.6164815160154546 1101 66-67 0.5466747101667195 1101 68-69 0.5462463184905957 1101 70-71 0.5962964061015796 1101 72-73 0.5452697294538424 1101 74-75 0.5153727361268565 1101 76-77 0.44174597088332135 1101 78-79 0.5829948323187111 1101 80-81 0.5614137148161049 1101 82-83 0.502544188688816 1101 84-85 0.522642094291875 1101 86-87 0.46320920262349574 1101 88-89 0.4968078303191703 1101 90-91 0.5331687125258391 1101 92-93 0.5049875660013434 1101 94-95 0.4239518799728579 1101 96-97 0.44214834019776106 1101 98-99 0.48415045952230784 1101 100 0.42456509027203637 1102 1 1.692131716907813 1102 2 1.1688857641206454 1102 3 0.7197267801403981 1102 4 0.5811163430194455 1102 5 0.44251268310100755 1102 6 0.40462346697218265 1102 7 0.35196508910154733 1102 8 0.39530027144251534 1102 9 0.4804427292794742 1102 10-11 0.45823430565445733 1102 12-13 0.3963727533867072 1102 14-15 0.5697000823643279 1102 16-17 0.5387452560308361 1102 18-19 0.5374257005331771 1102 20-21 0.5285349672715753 1102 22-23 0.5425537414054702 1102 24-25 0.5853936310417964 1102 26-27 0.5939355594290063 1102 28-29 0.5555074547987644 1102 30-31 0.5708718264027581 1102 32-33 0.536617030040162 1102 34-35 0.5364957532691861 1102 36-37 0.5744565357120379 1102 38-39 0.6474943055754423 1102 40-41 0.5888499524091699 1102 42-43 0.5883113780763054 1102 44-45 0.5926392023374802 1102 46-47 0.6005240270571974 1102 48-49 0.5690593151296071 1102 50-51 0.5581424289407906 1102 52-53 0.4861492180595448 1102 54-55 0.5423537755619918 1102 56-57 0.6213973366785552 1102 58-59 0.578982919535747 1102 60-61 0.5045681927097689 1102 62-63 0.5470764081615656 1102 64-65 0.5242433187543654 1102 66-67 0.5037172249350661 1102 68-69 0.5017912953575845 1102 70-71 0.4939303257798997 1102 72-73 0.4378949808864121 1102 74-75 0.43692117509976924 1102 76-77 0.3581871905806615 1102 78-79 0.48843047164570663 1102 80-81 0.4523398540171186 1102 82-83 0.42732987881019824 1102 84-85 0.44755092345599223 1102 86-87 0.4360072660014822 1102 88-89 0.44553655519752056 1102 90-91 0.4349879525976199 1102 92-93 0.38768954075136364 1102 94-95 0.38794195808902643 1102 96-97 0.3963320081251567 1102 98-99 0.4407180608480381 1102 100 0.36211960080703776 1103 1 1.6226999807216522 1103 2 1.0945085563397825 1103 3 0.6365180376994175 1103 4 0.5235826151551422 1103 5 0.384899499035221 1103 6 0.33749188995639656 1103 7 0.31124224431399483 1103 8 0.3255276403100993 1103 9 0.40500066143663105 1103 10-11 0.3722886314452083 1103 12-13 0.37221704850291104 1103 14-15 0.5276298489112747 1103 16-17 0.46448267650003316 1103 18-19 0.3896144282178966 1103 20-21 0.42402570514448 1103 22-23 0.44378382133419336 1103 24-25 0.4967240039142311 1103 26-27 0.4513357692352642 1103 28-29 0.4873509885115581 1103 30-31 0.47501293715600923 1103 32-33 0.5067304890420701 1103 34-35 0.47862951954949295 1103 36-37 0.4945263182968205 1103 38-39 0.5338310655723504 1103 40-41 0.5150299818296489 1103 42-43 0.4537337373475765 1103 44-45 0.48499638134898504 1103 46-47 0.5247686191142833 1103 48-49 0.4970644552283332 1103 50-51 0.417340223229985 1103 52-53 0.4136877549352178 1103 54-55 0.5182837458065919 1103 56-57 0.5250429815340141 1103 58-59 0.4916385260762439 1103 60-61 0.4791538972351077 1103 62-63 0.5140808879507048 1103 64-65 0.5012658898570024 1103 66-67 0.49325604827912173 1103 68-69 0.4925401196422001 1103 70-71 0.47966366750939926 1103 72-73 0.4398848077376414 1103 74-75 0.4065618911629585 1103 76-77 0.2580615820763512 1103 78-79 0.37673274625345243 1103 80-81 0.4370544040572284 1103 82-83 0.4197304641340267 1103 84-85 0.4169641706094538 1103 86-87 0.4262640945158438 1103 88-89 0.45737319782243446 1103 90-91 0.44147650119701964 1103 92-93 0.42601640489510473 1103 94-95 0.4076024978666304 1103 96-97 0.39997506956983386 1103 98-99 0.4302271434520719 1103 100 0.37752050764545686 1104 1 1.5823338442570538 1104 2 1.0633914798625526 1104 3 0.617918240144661 1104 4 0.5281157245037775 1104 5 0.3692964034426467 1104 6 0.3247166869535434 1104 7 0.27264948958722357 1104 8 0.2891997084302744 1104 9 0.38965711199553965 1104 10-11 0.35921880724584554 1104 12-13 0.345356015377412 1104 14-15 0.47269697887268336 1104 16-17 0.4653119917484787 1104 18-19 0.3901826338156056 1104 20-21 0.3913959143890011 1104 22-23 0.39419012761399586 1104 24-25 0.45871161820775797 1104 26-27 0.4291319402299436 1104 28-29 0.4493523350036597 1104 30-31 0.419088579057437 1104 32-33 0.45267643011058567 1104 34-35 0.3950068277802856 1104 36-37 0.43874267618568297 1104 38-39 0.5054341105208451 1104 40-41 0.5084721193234785 1104 42-43 0.46540240133044364 1104 44-45 0.4892849473846539 1104 46-47 0.48974581440396747 1104 48-49 0.49391566958912136 1104 50-51 0.4671382082046094 1104 52-53 0.4338299526745928 1104 54-55 0.4575737207276376 1104 56-57 0.49930316258123497 1104 58-59 0.4802029622593196 1104 60-61 0.45893269608465914 1104 62-63 0.5367533139850309 1104 64-65 0.4932587173730809 1104 66-67 0.5016115803203789 1104 68-69 0.4640611745425147 1104 70-71 0.5100993137100218 1104 72-73 0.4865598177386232 1104 74-75 0.4295607766840206 1104 76-77 0.4081062170512162 1104 78-79 0.4701535827982042 1104 80-81 0.44503107643319595 1104 82-83 0.4184063443798536 1104 84-85 0.45988844987241606 1104 86-87 0.45729276336884794 1104 88-89 0.46455798500579704 1104 90-91 0.49720017100196756 1104 92-93 0.48516988389415516 1104 94-95 0.46974234379707624 1104 96-97 0.4639942843349587 1104 98-99 0.5076201576971293 1104 100 0.42776560696893995 1105 1 1.5670525014502665 1105 2 1.0359971528731933 1105 3 0.6026995536614805 1105 4 0.4958365869578998 1105 5 0.33699629924613816 1105 6 0.2705756739559959 1105 7 0.2411462828074491 1105 8 0.27314283148425034 1105 9 0.34673762777745054 1105 10-11 0.3222569887650053 1105 12-13 0.28356723603341294 1105 14-15 0.40208613361786405 1105 16-17 0.35764348123274914 1105 18-19 0.3423186428011178 1105 20-21 0.33727105443433913 1105 22-23 0.3307132814697695 1105 24-25 0.39231705871124234 1105 26-27 0.3423042887254155 1105 28-29 0.3576502661254679 1105 30-31 0.3887558381072651 1105 32-33 0.3946475086688537 1105 34-35 0.37915099630991733 1105 36-37 0.4158877737732709 1105 38-39 0.46331009995575556 1105 40-41 0.4462345893381041 1105 42-43 0.3943666727232795 1105 44-45 0.4097942558719865 1105 46-47 0.4849090999699399 1105 48-49 0.43306242677652307 1105 50-51 0.39269533503919973 1105 52-53 0.35500804469335634 1105 54-55 0.44376494282997214 1105 56-57 0.42027425338718416 1105 58-59 0.45230369127624215 1105 60-61 0.4213484867458561 1105 62-63 0.45363593276790226 1105 64-65 0.43452500706219155 1105 66-67 0.4501101726478183 1105 68-69 0.47011464077044707 1105 70-71 0.45971945715352547 1105 72-73 0.36371163050149846 1105 74-75 0.2827660023104741 1105 76-77 0.2798278310187108 1105 78-79 0.3962900128786444 1105 80-81 0.3731288618863431 1105 82-83 0.36991334653756525 1105 84-85 0.3871310430188757 1105 86-87 0.39369079022215914 1105 88-89 0.41403345417464266 1105 90-91 0.3989513602354542 1105 92-93 0.3653782216127972 1105 94-95 0.36560721627431647 1105 96-97 0.35818795591121244 1105 98-99 0.3851390549495761 1105 100 0.3218546094974961 1106 1 1.420350609926306 1106 2 0.8830420275572877 1106 3 0.4823112356127197 1106 4 0.444574897877942 1106 5 0.2579793978684606 1106 6 0.2382090024793655 1106 7 0.16675806594044928 1106 8 0.19946921077556112 1106 9 0.2688040538883172 1106 10-11 0.24955772394245201 1106 12-13 0.22256824368298567 1106 14-15 0.35410462702724743 1106 16-17 0.2660071810701652 1106 18-19 0.21340994050888895 1106 20-21 0.21582916337604985 1106 22-23 0.212341879458549 1106 24-25 0.26258951718073575 1106 26-27 0.25503121109256455 1106 28-29 0.2655387171190071 1106 30-31 0.2654058085863298 1106 32-33 0.2519186575244632 1106 34-35 0.26153600338688676 1106 36-37 0.2852934861733303 1106 38-39 0.3441301134198298 1106 40-41 0.3025351838096668 1106 42-43 0.27197515710104625 1106 44-45 0.2669735901880159 1106 46-47 0.31417811495403924 1106 48-49 0.32514648855143236 1106 50-51 0.27892524528638063 1106 52-53 0.2323865238223206 1106 54-55 0.3024877265183221 1106 56-57 0.29830541952418343 1106 58-59 0.2557516321125064 1106 60-61 0.2553519635752366 1106 62-63 0.29043378311548196 1106 64-65 0.2817056507046374 1106 66-67 0.24701440612701475 1106 68-69 0.2240035630352608 1106 70-71 0.25422912877847637 1106 72-73 0.2370658603588538 1106 74-75 0.21328970701151917 1106 76-77 0.1356113241923893 1106 78-79 0.2659832740586481 1106 80-81 0.2523818983451207 1106 82-83 0.2519728508964292 1106 84-85 0.2558884951127638 1106 86-87 0.23201638808561142 1106 88-89 0.25583029054879347 1106 90-91 0.28991643298283876 1106 92-93 0.2722520955345793 1106 94-95 0.26026404339838294 1106 96-97 0.26665263988494203 1106 98-99 0.3015228750093186 1106 100 0.23308955727391378 1107 1 1.6001967934638763 1107 2 1.0479514060980222 1107 3 0.6007907208776473 1107 4 0.5044349463581099 1107 5 0.3482991872901309 1107 6 0.32846686879608455 1107 7 0.2691517248097597 1107 8 0.2769085230210848 1107 9 0.361727720736269 1107 10-11 0.3136691207580853 1107 12-13 0.3078995697628173 1107 14-15 0.4144175006404467 1107 16-17 0.39042370972998697 1107 18-19 0.33845009699435025 1107 20-21 0.3471622761156823 1107 22-23 0.3563891081068533 1107 24-25 0.42284151164712824 1107 26-27 0.3656636413081955 1107 28-29 0.35299517053135077 1107 30-31 0.3844907868165848 1107 32-33 0.3888789771907142 1107 34-35 0.41174340261243003 1107 36-37 0.4447017410505367 1107 38-39 0.468949736968149 1107 40-41 0.4503788348557407 1107 42-43 0.41355919871487856 1107 44-45 0.4404464294717343 1107 46-47 0.46080374071673447 1107 48-49 0.4605489625886392 1107 50-51 0.4800590805002898 1107 52-53 0.4536551849424981 1107 54-55 0.49632482774886455 1107 56-57 0.48429715592338596 1107 58-59 0.42710401425745204 1107 60-61 0.3716506690560806 1107 62-63 0.43571523404865076 1107 64-65 0.4267099615889691 1107 66-67 0.40844825005715535 1107 68-69 0.37908414233518073 1107 70-71 0.4132839223489242 1107 72-73 0.3887192198732663 1107 74-75 0.3814634593738191 1107 76-77 0.29743132630902736 1107 78-79 0.3922793991953242 1107 80-81 0.38275329133622193 1107 82-83 0.37378465149740236 1107 84-85 0.3782648229879513 1107 86-87 0.3341614293341095 1107 88-89 0.40812986936415285 1107 90-91 0.44731579983603 1107 92-93 0.422144098340123 1107 94-95 0.41667783550695603 1107 96-97 0.40628789195443815 1107 98-99 0.4190431666466701 1107 100 0.3483402760676384 1108 1 1.4060443994748688 1108 2 0.8839346691429739 1108 3 0.4606274483725059 1108 4 0.3887258047012594 1108 5 0.22274212122884762 1108 6 0.1704502680911517 1108 7 0.14827574022822176 1108 8 0.15638407133057797 1108 9 0.16340693767055825 1108 10-11 0.14641636436900285 1108 12-13 0.11161941608917658 1108 14-15 0.16537665894311004 1108 16-17 0.13189957715383116 1108 18-19 0.11444187859594024 1108 20-21 0.12084650251162543 1108 22-23 0.08121593834962937 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50-51 -1.5035534322833328 2316 52-53 -1.3341145788509792 2316 54-55 -1.2398958630913768 2316 56-57 -1.4063674301231828 2316 58-59 -1.2491887136085111 2316 60-61 -1.2279731824213371 2316 62-63 -2.253877641922692 2316 64-65 -1.5757106133447252 2316 66-67 -1.2686625478279296 2316 68-69 -1.2021089187321436 2316 70-71 -1.1309103935543732 2316 72-73 -1.0715299587155087 2316 74-75 -1.1644959372432027 2316 76-77 -1.0109670443234045 2316 78-79 -1.2995899367891823 2316 80-81 -1.1633816287264978 2316 82-83 -1.0669970496410066 2316 84-85 -1.00657098898348 2316 86-87 -1.1693652633646252 2316 88-89 -1.1021910270013606 2316 90-91 -1.054992185201204 2316 92-93 -1.022010083479774 2316 94-95 -1.0366920590037516 2316 96-97 -0.9985300828168491 2316 98-99 -0.9970849114037534 2316 100 -0.9931567905979826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 83.0 7 942.0 8 1280.0 9 495.0 10 255.0 11 201.0 12 306.0 13 1255.0 14 5410.0 15 14529.0 16 25606.0 17 35567.0 18 45266.0 19 58965.0 20 80686.0 21 116444.0 22 178802.0 23 285477.0 24 449570.0 25 573355.0 26 567476.0 27 491392.0 28 468893.0 29 512417.0 30 602557.0 31 733383.0 32 902204.0 33 1145433.0 34 1534764.0 35 2256298.0 36 3794039.0 37 7083945.0 38 9079636.0 39 2728576.0 40 6288.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.088725904292723 34.32588767435396 27.887985918718815 19.69740050263451 2 21.413711142347527 33.100117977745114 26.84018418796278 18.645986691944582 3 25.66750819487242 26.55837559845473 26.77777779422319 20.996338412449663 4 20.52260396465019 27.274779211702633 26.288789568464317 25.91382725518286 5 20.874891003352953 32.44670095784421 26.268305915450163 20.410102123352676 6 26.601984885646246 26.635461496347368 26.338162314939158 20.424391303067228 7 20.56436018275524 26.733310056496407 32.215339652614674 20.486990108133686 8 26.248285504071056 26.78209076811934 26.403869302978126 20.565754424831482 9 26.187030618118428 26.472595668761826 26.716810045173737 20.623563667946005 10-11 20.238853500827886 29.90013112091883 29.486437887625566 20.37457749062772 12-13 20.24202680763411 26.915684912539433 29.422279070724333 23.420009209102123 14-15 20.28378746600055 32.82864927692564 26.377874532717993 20.509688724355826 16-17 23.372988321076484 26.77444463800695 26.37947450690527 23.473092534011293 18-19 20.40041947472059 29.780579755530006 26.328909285949713 23.490091483799688 20-21 23.39692198600373 29.7796439022819 26.318494586321854 20.504939525392516 22-23 23.38071437589388 26.86463522734656 29.292151290362163 20.462499106397395 24-25 20.520094826476516 29.746827208808867 26.175805400670043 23.55727256404457 26-27 20.5470735738442 26.801053631029585 32.096205413497955 20.555667381628254 28-29 20.557033751462882 26.8733529405409 32.07332085225193 20.496292455744285 30-31 20.56972697868778 26.860303012317726 32.038470720694384 20.531499288300104 32-33 20.589009553814414 26.89120427141305 31.98642937712457 20.533356797647965 34-35 20.61269686823924 26.91985727815825 31.948336374665704 20.519109478936805 36-37 20.65198578109896 26.878203186065157 31.886014345892516 20.583796686943366 38-39 20.665651731437773 26.882871778762375 31.853423298753412 20.598053191046436 40-41 20.661167312788347 26.952722235529215 31.85500082728324 20.5311096243992 42-43 20.684942072284997 26.99514273292378 31.776938989369157 20.54297620542206 44-45 20.717541066120546 27.028652928571713 31.70649329817338 20.54731270713436 46-47 20.773234539694172 27.11076192539249 31.602837196459372 20.513166338453964 48-49 20.807021389033842 27.140146677877624 31.506459680752947 20.546372252335587 50-51 20.80678513382726 27.202587961948144 31.421278827842038 20.56934807638256 52-53 20.886425461190875 27.354645522026317 31.183489265287196 20.575439751495615 54-55 21.230121639855906 27.551724416595896 30.467762896873413 20.750391046674785 56-57 21.605404366055193 28.035238457355128 29.369162291380093 20.990194885209586 58-59 21.708272346942703 28.508282962130583 28.725137988931937 21.058306701994777 60-61 21.736449409691357 28.775768117598016 28.350300980331284 21.137481492379344 62-63 21.753015945469876 28.944876803843027 28.128060181704917 21.17404706898218 64-65 21.695335724662186 29.042258732704244 28.080019477958157 21.182386064675406 66-67 21.691271718872475 28.89468640991212 28.160964759597512 21.253077111617895 68-69 21.641151157652015 28.77352563203691 28.297504817043805 21.287818393267273 70-71 21.573667257919794 28.72135024707726 28.392830075639164 21.312152419363777 72-73 21.550858569087783 28.661186670959964 28.420398502398626 21.36755625755363 74-75 21.56552638225044 28.694284498098078 28.30258149601614 21.437607623635348 76-77 21.632976139248015 28.773955320863013 28.116263331909895 21.476805207979073 78-79 21.711863325011503 28.876408346113493 27.756349232240186 21.655379096634817 80-81 21.778342998353555 29.00730891482756 27.451933000343615 21.76241508647527 82-83 21.770577921784763 29.148803739576618 27.176298449712803 21.904319888925812 84-85 21.79743447905892 29.21606539830534 26.96928636066676 22.017213761968975 86-87 21.837643303853717 29.218442565280316 26.780830039265354 22.163084091600616 88-89 21.922282013006285 29.24296085015242 26.590717980332933 22.24403915650836 90-91 21.93426169574965 29.19774681341767 26.450643799630154 22.417347691202526 92-93 21.94528196300229 29.191874557992765 26.39402601228363 22.46881746672132 94-95 21.970960954163928 29.192995430371198 26.35036222580703 22.48568138965785 96-97 22.017736131395225 29.109743445339525 26.296487095949306 22.57603332731594 98-99 22.04980810103501 29.031435070501498 26.279032242522366 22.639724585941128 100 22.055556174116493 29.039877138952786 26.210777360372862 22.693789326557866 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 58785.0 1 36885.0 2 11770.5 3 8109.5 4 7630.0 5 7574.0 6 7766.5 7 8179.5 8 8536.0 9 9011.5 10 10040.5 11 13200.5 12 115891.5 13 142782.0 14 68050.5 15 71652.0 16 82341.0 17 93259.5 18 103590.0 19 114714.5 20 129640.0 21 151471.0 22 181032.0 23 214732.0 24 247966.0 25 285736.0 26 326802.5 27 373534.0 28 436506.0 29 499120.5 30 551519.0 31 614041.0 32 678498.5 33 728596.5 34 778615.5 35 843026.0 36 909247.0 37 955395.0 38 993016.0 39 1020399.5 40 1043882.5 41 1086856.0 42 1134116.5 43 1142790.0 44 1123992.0 45 1125550.0 46 1128464.0 47 1117243.0 48 1107301.0 49 1100141.0 50 1090144.0 51 1076688.5 52 1055507.5 53 1009339.0 54 947029.5 55 881156.0 56 806878.0 57 728177.0 58 644410.5 59 550325.5 60 456295.5 61 372896.0 62 294795.0 63 223004.0 64 164870.0 65 119856.0 66 86178.0 67 64405.0 68 49556.0 69 38010.0 70 29912.0 71 24302.5 72 20251.0 73 16493.5 74 13715.0 75 12289.0 76 9501.0 77 5600.0 78 3230.0 79 2253.0 80 1530.0 81 1080.5 82 802.0 83 543.5 84 359.0 85 269.5 86 176.5 87 97.0 88 77.0 89 65.5 90 40.0 91 26.0 92 19.5 93 12.0 94 6.5 95 5.5 96 4.5 97 2.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10657219369189824 2 0.0 3 0.0 4 0.0 5 0.009570243381087357 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.364374658007368E-5 20-21 0.010807596221574371 22-23 0.0 24-25 8.924925392508006E-4 26-27 0.004909449009444288 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.4800871297691553E-6 40-41 0.007560285058860844 42-43 0.03882120532671517 44-45 0.015120570117721688 46-47 0.004788081864803217 48-49 0.0016547374110819156 50-51 0.0 52-53 0.0014800871297691552 54-55 6.512383370984284E-5 56-57 8.688111451744942E-4 58-59 2.812165546561395E-5 60-61 3.0341786160267684E-4 62-63 2.9601742595383105E-4 64-65 5.920348519076621E-5 66-67 3.404200398469057E-4 68-69 5.446720637550492E-4 70-71 4.603070973582073E-4 72-73 0.0013098771098457025 74-75 0.0017420625517382956 76-77 0.002547229950332716 78-79 0.00464451341321561 80-81 0.004358856597170163 82-83 0.007607647847013457 84-85 0.0025413096018136396 86-87 0.002946853475370388 88-89 0.005831543291290472 90-91 0.005895187037870546 92-93 0.005761979196191321 94-95 0.0027618425841492438 96-97 0.0057338575407257075 98-99 0.007967309019547363 100 0.30036888211535234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.3781795E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.20221868294275 #Duplication Level Percentage of deduplicated Percentage of total 1 67.32409722614392 26.39253982090994 2 17.106326141639798 13.412118765326072 3 6.023563116155524 7.084111155901109 4 2.750478298604476 4.31299406978324 5 1.5579991616499191 3.0538511921420803 6 1.014883610353589 2.3871413538489517 7 0.6991702216189389 1.9186316747155034 8 0.5235778813315498 1.6420331681209013 9 0.39628841417187355 1.398184656649414 >10 2.2568153391357266 16.279752743646828 >50 0.20226323544719252 5.548857174283093 >100 0.14358418617267485 10.400570577469932 >500 4.042082409244612E-4 0.10396901423208581 >1k 5.040702329064238E-4 0.42710661433351566 >5k 7.481516829121848E-6 0.019515653593573462 >10k+ 3.7407584132512443E-5 5.618622365043835 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1736731 5.14102640194223 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94262 0.27903194605260023 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0035906913768199706 0.0 0.0 0.0 1.1840697038153242E-5 2 0.0036173329451558156 0.0 0.0 0.0 1.1840697038153242E-5 3 0.00367949660460612 5.920348519076621E-6 0.0 0.0 1.4800871297691552E-5 4 0.0036824567788656584 2.3681394076306485E-5 0.0 0.0 1.7761045557229862E-5 5 0.0036824567788656584 2.3681394076306485E-5 0.0 0.0 2.6641568335844794E-5 6 0.0036854169531251968 3.2561916854921414E-5 0.0 8.880522778614931E-6 2.6641568335844794E-5 7 0.0036883771273847348 3.2561916854921414E-5 0.0 1.4800871297691552E-5 2.9601742595383104E-5 8 0.0036942974759038116 3.2561916854921414E-5 0.0 1.7761045557229862E-5 2.9601742595383104E-5 9 0.0037031779986824265 3.2561916854921414E-5 0.0 1.7761045557229862E-5 3.5522091114459723E-5 10-11 0.0037046580858121955 3.2561916854921414E-5 0.0 1.7761045557229862E-5 4.144243963353635E-5 12-13 0.003719458957109887 3.2561916854921414E-5 0.0 1.7761045557229862E-5 4.736278815261297E-5 14-15 0.0037298195670182714 3.7002178244228875E-5 0.0 2.220130694653733E-5 6.0683572320535366E-5 16-17 0.003759421309613654 3.848226537399803E-5 0.0 2.3681394076306485E-5 6.95640950991503E-5 18-19 0.0037860628779494993 4.440261389307466E-5 0.0 2.6641568335844794E-5 8.28848792670727E-5 20-21 0.0038215849690639587 5.62433109312279E-5 0.0 3.848226537399803E-5 8.880522778614932E-5 22-23 0.003944432200834799 6.660392083961199E-5 0.0 3.848226537399803E-5 9.324548917545678E-5 24-25 0.004497984787368462 6.808400796938114E-5 0.0 4.144243963353635E-5 1.0212601195407172E-4 26-27 0.006419137881808826 7.104418222891945E-5 0.0 4.292252676330551E-5 1.0360609908384087E-4 28-29 0.011632004752855791 8.140479213730354E-5 0.0 4.736278815261297E-5 1.0656627334337918E-4 30-31 0.02356298710592495 8.732514065638016E-5 0.0 5.476322380145875E-5 1.1988705751130157E-4 32-33 0.04700756724146837 1.0064592482430256E-4 0.0 5.62433109312279E-5 1.3764810306853145E-4 34-35 0.08777212696957044 1.0360609908384087E-4 0.0 5.920348519076621E-5 1.4800871297691553E-4 36-37 0.14933191087092915 1.154467961219941E-4 0.0 6.364374658007367E-5 1.5096888723645384E-4 38-39 0.23749922110414795 1.2432731890060905E-4 0.0 6.512383370984283E-5 1.554091486257613E-4 40-41 0.3550344201662463 1.2580740603037818E-4 0.0 6.808400796938114E-5 1.6724984566391456E-4 42-43 0.5021136384256668 1.3320784167922397E-4 0.0 7.104418222891945E-5 1.71690107053222E-4 44-45 0.6833384667688618 1.391281901983006E-4 0.0 7.696453074799607E-5 1.7317019418299116E-4 46-47 0.9012442944491257 1.4356845158760805E-4 0.0 7.696453074799607E-5 1.8649097835091356E-4 48-49 1.1597740735801636 1.4800871297691553E-4 0.0 7.992470500753438E-5 1.953715011295285E-4 50-51 1.4591971800195935 1.6132949714483792E-4 0.0 7.992470500753438E-5 2.1609272094629667E-4 52-53 1.796565280204915 1.71690107053222E-4 0.0 8.436496639684186E-5 2.2349315659514243E-4 54-55 2.17122861588616 1.8353080409137524E-4 0.0 9.62056634349951E-5 2.3089359224398823E-4 56-57 2.5869170066303466 1.850108912211444E-4 0.0 1.0212601195407172E-4 2.3977411502260315E-4 58-59 3.0429925348845437 1.924113268699902E-4 0.0 1.0656627334337918E-4 2.486546378012181E-4 60-61 3.5351969899764057 2.0425202390814343E-4 0.0 1.095264476029175E-4 2.6197542196914047E-4 62-63 4.0613798053063785 2.057321110379126E-4 0.0 1.1544679612199411E-4 2.723360318775246E-4 64-65 4.620725452865959 2.1017237242722004E-4 0.0 1.1840697038153242E-4 2.752962061370629E-4 66-67 5.2150781212188395 2.2053298233560415E-4 0.0 1.2580740603037818E-4 2.797364675263703E-4 68-69 5.841477636105482 2.3385376650352652E-4 0.0 1.3024766741968565E-4 2.841767289156778E-4 70-71 6.504753521830324 2.3681394076306483E-4 0.0 1.3024766741968565E-4 2.900970774347544E-4 72-73 7.198578405913599 2.412542021523723E-4 1.4800871297691553E-6 1.3024766741968565E-4 2.945373388240619E-4 74-75 7.919977313224475 2.5013472493098726E-4 2.9601742595383106E-6 1.317277545494548E-4 2.9601742595383105E-4 76-77 8.670482133942262 2.5309489919052557E-4 2.9601742595383106E-6 1.3320784167922397E-4 2.974975130836002E-4 78-79 9.450300080265125 2.708559447477554E-4 2.9601742595383106E-6 1.4356845158760805E-4 3.0341786160267684E-4 80-81 10.25461050841141 2.752962061370629E-4 2.9601742595383106E-6 1.450485387173772E-4 3.182187329003684E-4 82-83 11.079809406220125 2.8269664178590863E-4 2.9601742595383106E-6 1.4800871297691553E-4 3.1969882003013753E-4 84-85 11.924617978411153 2.900970774347544E-4 2.9601742595383106E-6 1.5688923575553045E-4 3.1969882003013753E-4 86-87 12.795054259254133 3.1229838438129174E-4 2.9601742595383106E-6 1.6280958427460708E-4 3.285793428087525E-4 88 13.460628720291506 3.167386457705992E-4 2.9601742595383106E-6 1.657697585341454E-4 3.285793428087525E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 220020 0.0 85.973015 8 GTGGTAT 223325 0.0 85.016335 6 AGTGGTA 224770 0.0 84.49941 5 TGGTATC 223950 0.0 84.46222 7 GTATCAA 224325 0.0 84.17229 9 GCAGTGG 232170 0.0 81.85271 3 AAGCAGT 229145 0.0 81.172455 1 CAGTGGT 233920 0.0 81.01332 4 AGCAGTG 241930 0.0 78.43014 2 CAACGCA 215155 0.0 44.58863 12-13 ATCAACG 217300 0.0 44.247974 10-11 ACGCAGA 218920 0.0 43.945873 14-15 TCAACGC 214425 0.0 43.937214 12-13 AACGCAG 215875 0.0 43.703526 14-15 AGAGTAC 220260 0.0 43.538322 18-19 GAGTACT 215310 0.0 43.350582 20-21 CGCAGAG 220000 0.0 42.962063 16-17 AGTACTT 225380 0.0 42.233273 20-21 TATCAAC 223240 0.0 42.23175 10-11 GTACTTT 225970 0.0 41.322067 22-23 >>END_MODULE