FastQCFastQC Report
Thu 26 May 2016
SRR1660764_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660764_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32371765
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23887617.379149700363882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA753070.23263173941859516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2981000.086.952988
GTGGTAT2998200.086.416556
TGGTATC2995950.086.164757
AGTGGTA3011200.086.0579155
GTATCAA3019100.085.766989
AAGCAGT3057850.085.2878651
GCAGTGG3113550.083.985283
CAGTGGT3112450.083.173884
AGCAGTG3181000.082.238712
CAACGCA2960900.044.76629612-13
ATCAACG2982350.044.55220810-11
ACGCAGA2983650.044.4344114-15
GAGTACT2867650.044.33720820-21
AGAGTAC2946600.044.2640618-19
TCAACGC2960350.044.07409312-13
AACGCAG2974200.043.93969314-15
AGTACTT2954950.043.7546120-21
CGCAGAG3000300.043.48637416-17
TATCAAC3014200.042.9563210-11
GTACTTT2968450.042.8155422-23
Produced by FastQC (version 0.11.5)