FastQCFastQC Report
Thu 26 May 2016
SRR1660764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32371765
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26025328.039512210718199No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA546270.16874890819206181No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT504990.15599705484084664No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT382810.1182542873395998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3213100.087.230848
GTGGTAT3247200.086.640416
TGGTATC3240150.086.5098657
AGTGGTA3258350.086.438665
GTATCAA3243400.086.272499
AAGCAGT3215900.085.789691
GCAGTGG3334800.084.562773
CAGTGGT3343250.084.065074
AGCAGTG3397900.082.89422
CAACGCA3169850.044.97918312-13
ATCAACG3187400.044.7949210-11
ACGCAGA3201100.044.65789414-15
AGAGTAC3212200.044.31337718-19
GAGTACT3132500.044.21156320-21
TCAACGC3167900.044.12870412-13
AACGCAG3183100.044.02150714-15
AGTACTT3219000.043.85799820-21
CGCAGAG3225400.043.51558716-17
TATCAAC3240800.043.1295310-11
GTACTTT3230050.042.8276222-23
Produced by FastQC (version 0.11.5)