##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660764_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32371765 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.063258954215193 33.0 31.0 34.0 27.0 34.0 2 31.825778575866963 34.0 31.0 34.0 28.0 34.0 3 32.317732134778566 34.0 31.0 34.0 30.0 34.0 4 35.846915668639014 37.0 35.0 37.0 35.0 37.0 5 35.89548490791281 37.0 35.0 37.0 35.0 37.0 6 35.939583708209916 37.0 35.0 37.0 35.0 37.0 7 35.8840208434727 37.0 35.0 37.0 35.0 37.0 8 35.92507634353579 37.0 35.0 37.0 35.0 37.0 9 37.644209297824816 39.0 37.0 39.0 35.0 39.0 10-11 37.52706468121216 39.0 37.0 39.0 34.5 39.0 12-13 37.656044627161975 39.0 37.0 39.0 35.0 39.0 14-15 39.004055046118125 40.0 38.0 41.0 36.0 41.0 16-17 39.01818314509573 40.0 38.0 41.0 36.0 41.0 18-19 38.99184930138965 40.0 38.0 41.0 35.0 41.0 20-21 38.90039701264358 40.0 38.0 41.0 35.0 41.0 22-23 38.7448585055526 40.0 38.0 41.0 34.5 41.0 24-25 38.73125713102144 40.0 38.0 41.0 34.5 41.0 26-27 38.74471489274681 40.0 38.0 41.0 35.0 41.0 28-29 38.66904127717473 40.0 38.0 41.0 34.0 41.0 30-31 38.58186073573684 40.0 38.0 41.0 34.0 41.0 32-33 38.48548010897768 40.0 38.0 41.0 34.0 41.0 34-35 38.38239668118189 40.0 38.0 41.0 34.0 41.0 36-37 38.17413381692349 40.0 38.0 41.0 33.5 41.0 38-39 37.6194157933619 40.0 35.5 41.0 33.0 41.0 40-41 37.46928701601534 39.5 35.0 41.0 33.0 41.0 42-43 37.29797494823035 39.0 35.0 41.0 33.0 41.0 44-45 37.17769109901793 39.0 35.0 41.0 32.0 41.0 46-47 37.041418980398504 39.0 35.0 41.0 32.0 41.0 48-49 37.1450125750017 39.0 35.0 41.0 32.0 41.0 50-51 37.02211380812878 39.0 35.0 41.0 31.5 41.0 52-53 36.679344113612586 39.0 35.0 41.0 30.5 41.0 54-55 35.98194423442774 39.0 35.0 41.0 27.5 41.0 56-57 35.45925446141105 39.0 35.0 41.0 24.0 41.0 58-59 35.20779370540964 39.0 35.0 41.0 23.0 41.0 60-61 35.02538224282797 38.5 35.0 41.0 23.0 41.0 62-63 34.76056935727786 38.0 34.0 40.0 22.5 41.0 64-65 34.53888762321115 37.0 34.0 40.0 22.5 41.0 66-67 34.30890725297184 37.0 34.0 40.0 23.0 41.0 68-69 34.05852651222446 36.5 34.0 39.5 23.0 41.0 70-71 33.76925258786477 36.0 33.5 39.0 23.0 41.0 72-73 33.47660623385842 35.0 33.0 39.0 23.0 40.5 74-75 33.132576181743566 35.0 33.0 37.5 22.5 40.0 76-77 31.064247207404353 33.5 30.0 35.5 19.5 38.5 78-79 32.32156839455618 35.0 32.5 37.0 20.0 39.0 80-81 32.30635297457522 35.0 33.0 36.0 20.0 39.0 82-83 32.08468135734953 35.0 33.0 36.0 20.0 37.5 84-85 31.852292561125413 35.0 33.0 35.5 19.5 37.0 86-87 31.65475856815345 35.0 33.0 35.0 19.0 37.0 88-89 31.44586520073898 35.0 32.5 35.0 19.0 36.0 90-91 31.291812510068574 35.0 32.0 35.0 18.5 36.0 92-93 31.16564416552511 35.0 32.0 35.0 18.0 36.0 94-95 31.068409831839567 35.0 32.0 35.0 18.0 35.5 96-97 30.968437803746568 35.0 32.0 35.0 17.0 35.0 98-99 30.859309648392667 35.0 32.0 35.0 17.0 35.0 100 30.67803327992774 35.0 32.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7032710310388808 1101 2 1.184995218176713 1101 3 0.7398095995769154 1101 4 0.5932699643645165 1101 5 0.46302428724331435 1101 6 0.42779430823804887 1101 7 0.3827662515542585 1101 8 0.3952577014795722 1101 9 0.4766360658174591 1101 10-11 0.4586124607306914 1101 12-13 0.4231608813335228 1101 14-15 0.5784221801960072 1101 16-17 0.5561097574786302 1101 18-19 0.47693896589266416 1101 20-21 0.5355092361945424 1101 22-23 0.5152231710558297 1101 24-25 0.5472260054712379 1101 26-27 0.5304828135067297 1101 28-29 0.5441831491599558 1101 30-31 0.5267650513798046 1101 32-33 0.534040399939613 1101 34-35 0.53740369225725 1101 36-37 0.49564325223690275 1101 38-39 0.5873250182004668 1101 40-41 0.5762194986092908 1101 42-43 0.5572256793861641 1101 44-45 0.5239521971374259 1101 46-47 0.4973951393394458 1101 48-49 0.46071315994601036 1101 50-51 0.44808188682224426 1101 52-53 0.4539793065086499 1101 54-55 0.5061844962026996 1101 56-57 0.5320399988828655 1101 58-59 0.5030434550811265 1101 60-61 0.4916309709439517 1101 62-63 0.5405695622161772 1101 64-65 0.4831236442940394 1101 66-67 0.4460703062435343 1101 68-69 0.4843446051979825 1101 70-71 0.4835922513596458 1101 72-73 0.454860520264603 1101 74-75 0.4320465215745273 1101 76-77 0.34355768866246805 1101 78-79 0.4715927047751549 1101 80-81 0.44910462275294805 1101 82-83 0.4195576471467888 1101 84-85 0.44262666964224806 1101 86-87 0.4171220536540794 1101 88-89 0.45351920794885814 1101 90-91 0.483767485338781 1101 92-93 0.3927570831151037 1101 94-95 0.3618110984409135 1101 96-97 0.352551108989104 1101 98-99 0.37255703382900407 1101 100 0.3129858384480464 1102 1 1.655940837783902 1102 2 1.1457162547855368 1102 3 0.7084659847169021 1102 4 0.5619348073301964 1102 5 0.43575192430769505 1102 6 0.38961133920821567 1102 7 0.3765538016401777 1102 8 0.39394073593320655 1102 9 0.4798355366307021 1102 10-11 0.47293005534714894 1102 12-13 0.384201117257021 1102 14-15 0.5472300759434319 1102 16-17 0.5451447002552428 1102 18-19 0.5258598536189467 1102 20-21 0.5297275674823894 1102 22-23 0.5326806322238795 1102 24-25 0.5547769072139701 1102 26-27 0.552136979077325 1102 28-29 0.5214526331514264 1102 30-31 0.5321191432567858 1102 32-33 0.5123547719870629 1102 34-35 0.49217538056726795 1102 36-37 0.5482148591868921 1102 38-39 0.6169152461402874 1102 40-41 0.6003629184180994 1102 42-43 0.6199139531095454 1102 44-45 0.6141688566189671 1102 46-47 0.6290065745639737 1102 48-49 0.5782895509121815 1102 50-51 0.5572533964990924 1102 52-53 0.49991947951979654 1102 54-55 0.6055069237946569 1102 56-57 0.6676641008438793 1102 58-59 0.6210200787502416 1102 60-61 0.5959310806870022 1102 62-63 0.6388423870159627 1102 64-65 0.6125857982553597 1102 66-67 0.5847781734084876 1102 68-69 0.5803614834525419 1102 70-71 0.564711035080002 1102 72-73 0.4979453404409284 1102 74-75 0.5173729148810438 1102 76-77 0.4485552789908347 1102 78-79 0.5723197017736723 1102 80-81 0.5610192400065657 1102 82-83 0.5571244344494914 1102 84-85 0.5241698562329908 1102 86-87 0.49659216332229406 1102 88-89 0.5171909985045566 1102 90-91 0.5023849613187537 1102 92-93 0.48434930488366845 1102 94-95 0.43408432261546537 1102 96-97 0.4540075706525286 1102 98-99 0.49378664731639077 1102 100 0.3989951777701073 1103 1 1.582231173614737 1103 2 1.061888383019184 1103 3 0.6409962666931364 1103 4 0.5267491058577392 1103 5 0.40379173951447456 1103 6 0.3622475291968925 1103 7 0.3473820396664351 1103 8 0.35734126976002045 1103 9 0.4267007260133582 1103 10-11 0.39753319438769097 1103 12-13 0.3663030230980411 1103 14-15 0.4927202357166749 1103 16-17 0.44285525888194854 1103 18-19 0.38455491332798886 1103 20-21 0.4159909215428428 1103 22-23 0.4486219520350616 1103 24-25 0.5042284698926025 1103 26-27 0.4484768670718964 1103 28-29 0.44709400237340446 1103 30-31 0.4240033962298213 1103 32-33 0.4316701759949524 1103 34-35 0.445338989647901 1103 36-37 0.4555374584378242 1103 38-39 0.5096396350543486 1103 40-41 0.4806076381657931 1103 42-43 0.4567667446406958 1103 44-45 0.4786472038213887 1103 46-47 0.4851950774165701 1103 48-49 0.4692814154227847 1103 50-51 0.38325606904211895 1103 52-53 0.40298211582438626 1103 54-55 0.46660987105630625 1103 56-57 0.5367693287684574 1103 58-59 0.4876618588386066 1103 60-61 0.47414987200484404 1103 62-63 0.5002846789342996 1103 64-65 0.48149712989917504 1103 66-67 0.42679412178156184 1103 68-69 0.47172742067238005 1103 70-71 0.49995997553683225 1103 72-73 0.4531692989203506 1103 74-75 0.4083367071178614 1103 76-77 0.27575060797425976 1103 78-79 0.41477167553951944 1103 80-81 0.46675369246273135 1103 82-83 0.40596831704995395 1103 84-85 0.40437932002031474 1103 86-87 0.4063814043091476 1103 88-89 0.4220947564034887 1103 90-91 0.4374740411715905 1103 92-93 0.40815353539740684 1103 94-95 0.3950852969515495 1103 96-97 0.39519215923552764 1103 98-99 0.3757037893366224 1103 100 0.3277118031684907 1104 1 1.573706361997381 1104 2 1.0711639865345042 1104 3 0.6482347017175982 1104 4 0.5206450304468078 1104 5 0.3975230142843813 1104 6 0.3486615985777206 1104 7 0.295656811014851 1104 8 0.3095526942332185 1104 9 0.391157445110494 1104 10-11 0.38823512502045787 1104 12-13 0.35438402442433414 1104 14-15 0.49486191628748344 1104 16-17 0.4692967717354861 1104 18-19 0.40557585680233643 1104 20-21 0.382140140802548 1104 22-23 0.4124336665261765 1104 24-25 0.45234659402767363 1104 26-27 0.3988053261112938 1104 28-29 0.41694952332821344 1104 30-31 0.38768444920479794 1104 32-33 0.3979182317738008 1104 34-35 0.3514563882047028 1104 36-37 0.39192022526821546 1104 38-39 0.5157861654818774 1104 40-41 0.4955529892165558 1104 42-43 0.4927182853101755 1104 44-45 0.4592177704437077 1104 46-47 0.5113937382930445 1104 48-49 0.4966360353579091 1104 50-51 0.48138578134076226 1104 52-53 0.48714792185308653 1104 54-55 0.5661503321531782 1104 56-57 0.6294291472258138 1104 58-59 0.5638323438195982 1104 60-61 0.5517928082604158 1104 62-63 0.6160238618055232 1104 64-65 0.5821564572177422 1104 66-67 0.5462503003449655 1104 68-69 0.5461925980684228 1104 70-71 0.5545175692801791 1104 72-73 0.510663761160508 1104 74-75 0.5026164568504825 1104 76-77 0.4499007444670511 1104 78-79 0.5072155613543643 1104 80-81 0.46499368305496347 1104 82-83 0.46929737399894833 1104 84-85 0.5063582130585864 1104 86-87 0.5061496566524717 1104 88-89 0.49546624108647563 1104 90-91 0.4908424335956454 1104 92-93 0.5003627691952239 1104 94-95 0.5029077946785456 1104 96-97 0.46400975349486373 1104 98-99 0.5024753049300514 1104 100 0.4317972770015217 1105 1 1.5328138803621556 1105 2 1.0244392232473096 1105 3 0.5990445321174036 1105 4 0.4961912937670192 1105 5 0.3395088447954677 1105 6 0.2831178325132555 1105 7 0.22500548420636335 1105 8 0.2751045559042282 1105 9 0.32425276566142003 1105 10-11 0.3205992410283329 1105 12-13 0.2893965672520764 1105 14-15 0.39187409310671484 1105 16-17 0.3187600811206437 1105 18-19 0.276785432718647 1105 20-21 0.30281427083197343 1105 22-23 0.3012863754311681 1105 24-25 0.33958345594047756 1105 26-27 0.30679038911598155 1105 28-29 0.32814188137461997 1105 30-31 0.3544811119429383 1105 32-33 0.33494617763859935 1105 34-35 0.3183580078330053 1105 36-37 0.3603840028400711 1105 38-39 0.41612487772989226 1105 40-41 0.430561688987396 1105 42-43 0.38899033649442316 1105 44-45 0.3929262413795982 1105 46-47 0.43937271513058107 1105 48-49 0.42273905606238316 1105 50-51 0.418824186807214 1105 52-53 0.38154222943595784 1105 54-55 0.4764396124521326 1105 56-57 0.47362480370377824 1105 58-59 0.49600095954172474 1105 60-61 0.39713625502454164 1105 62-63 0.42120729895501796 1105 64-65 0.3653102978566878 1105 66-67 0.41352848592551794 1105 68-69 0.4470623509063998 1105 70-71 0.4244609838828737 1105 72-73 0.3536665750256489 1105 74-75 0.3124660394408778 1105 76-77 0.2810819578109509 1105 78-79 0.41737663955006354 1105 80-81 0.40006331112115134 1105 82-83 0.37548560884342663 1105 84-85 0.413048547755853 1105 86-87 0.40139336918388224 1105 88-89 0.41328687292988064 1105 90-91 0.4330769740745879 1105 92-93 0.4000934822169917 1105 94-95 0.3651822714248212 1105 96-97 0.3644986374568404 1105 98-99 0.3772345410713882 1105 100 0.3547540122081756 1106 1 1.403234885302581 1106 2 0.8851872097490379 1106 3 0.49329542079750155 1106 4 0.44101878458790367 1106 5 0.2684027813429424 1106 6 0.2484471094675058 1106 7 0.18430848208787154 1106 8 0.2015762771547358 1106 9 0.246638165616055 1106 10-11 0.22386259021310906 1106 12-13 0.1921729721743688 1106 14-15 0.31684868070552596 1106 16-17 0.26479230486405925 1106 18-19 0.18647230765422051 1106 20-21 0.20466045783638265 1106 22-23 0.19580899165047327 1106 24-25 0.24885671581518665 1106 26-27 0.217563921790763 1106 28-29 0.25183767039496274 1106 30-31 0.2613231720615019 1106 32-33 0.24355956356478714 1106 34-35 0.24040142543638154 1106 36-37 0.261959968302385 1106 38-39 0.3399212925038384 1106 40-41 0.33249435974251185 1106 42-43 0.2961618606587848 1106 44-45 0.27191760308232205 1106 46-47 0.30801987528706576 1106 48-49 0.2758216283992141 1106 50-51 0.2519977104970721 1106 52-53 0.20739023532184575 1106 54-55 0.23297898606094947 1106 56-57 0.23750520272702147 1106 58-59 0.21192470876244585 1106 60-61 0.23621867509778127 1106 62-63 0.25848087517420737 1106 64-65 0.2538396350583838 1106 66-67 0.2347417559513829 1106 68-69 0.23267333515955357 1106 70-71 0.25512970634429877 1106 72-73 0.24017318605985594 1106 74-75 0.1923266654123168 1106 76-77 0.15390300075204166 1106 78-79 0.28790677161192235 1106 80-81 0.25274709533092476 1106 82-83 0.25781055847145495 1106 84-85 0.2674716532077248 1106 86-87 0.2481519694578047 1106 88-89 0.2933063362170927 1106 90-91 0.3021222955683811 1106 92-93 0.24850442034673392 1106 94-95 0.24320919513617056 1106 96-97 0.25662616334391686 1106 98-99 0.29628550556373767 1106 100 0.22474236204905296 1107 1 1.5273032192062956 1107 2 1.0092063506059752 1107 3 0.5818841299534441 1107 4 0.4798445531651794 1107 5 0.3315616483671775 1107 6 0.2879950663811286 1107 7 0.24023483872972662 1107 8 0.2652863299076458 1107 9 0.3208691524303404 1107 10-11 0.2854730507680898 1107 12-13 0.2754401357330849 1107 14-15 0.37624542519113646 1107 16-17 0.32373478846324844 1107 18-19 0.28758835462796384 1107 20-21 0.28677306632216926 1107 22-23 0.35442293845562034 1107 24-25 0.383645944941982 1107 26-27 0.3290117685370433 1107 28-29 0.30306346255500927 1107 30-31 0.3272845250203247 1107 32-33 0.33451972862333434 1107 34-35 0.37125128904833815 1107 36-37 0.43929564056465864 1107 38-39 0.46737187429547333 1107 40-41 0.45093713681570335 1107 42-43 0.4111262687624375 1107 44-45 0.40449946657280833 1107 46-47 0.47452804615104327 1107 48-49 0.442423543820162 1107 50-51 0.417569101971381 1107 52-53 0.395045746529604 1107 54-55 0.43398653838406887 1107 56-57 0.4044227339468591 1107 58-59 0.3998433914743771 1107 60-61 0.34214027362155264 1107 62-63 0.39640382650676287 1107 64-65 0.3584438018490559 1107 66-67 0.34189792645285166 1107 68-69 0.31568321276940026 1107 70-71 0.34594906267860637 1107 72-73 0.35596743718442525 1107 74-75 0.35649249099054714 1107 76-77 0.30107120941536536 1107 78-79 0.3877116272211012 1107 80-81 0.38016117770080626 1107 82-83 0.37423609477784225 1107 84-85 0.37246878798211824 1107 86-87 0.36895969704128717 1107 88-89 0.4090806758013841 1107 90-91 0.4174955426696414 1107 92-93 0.39907119685923576 1107 94-95 0.36874297293030267 1107 96-97 0.3395994836033225 1107 98-99 0.3724260480477106 1107 100 0.2994930155897144 1108 1 1.3576143191238046 1108 2 0.8637957310900042 1108 3 0.4325153625670879 1108 4 0.37114924563055496 1108 5 0.2012777884448198 1108 6 0.17485102683988885 1108 7 0.09495218163469588 1108 8 0.11281683086988181 1108 9 0.16263156374159848 1108 10-11 0.10430051077479163 1108 12-13 0.10754775671137651 1108 14-15 0.1480948094278105 1108 16-17 0.11210942514072997 1108 18-19 0.07769715618552198 1108 20-21 0.10749519446936517 1108 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271.0 12 409.0 13 985.0 14 3573.0 15 9176.0 16 17041.0 17 25850.0 18 37429.0 19 55006.0 20 83493.0 21 131097.0 22 211284.0 23 353249.0 24 586326.0 25 766819.0 26 740960.0 27 590744.0 28 494711.0 29 498734.0 30 569438.0 31 688503.0 32 850289.0 33 1077230.0 34 1459413.0 35 2182742.0 36 3708515.0 37 6662577.0 38 8206210.0 39 2350144.0 40 6490.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.749583698548022 35.68901446584151 28.298343230311247 19.263058605299218 2 21.252193076281138 35.674245750888154 25.20389604953576 17.869665123294943 3 28.63934048699538 25.44238165574228 25.30535483622842 20.612923021033914 4 19.980841328855565 26.546640258879922 25.03620979578963 28.436308616474882 5 20.49166767355902 34.51578693418378 25.24941555189446 19.743129840362737 6 29.619608322252432 25.54722301981372 25.087180139853356 19.745988518080495 7 20.0800512421859 25.48225591035892 34.34062368857552 20.09706915887966 8 29.385493809188347 25.812086551351154 24.97005337830668 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25.614398844177945 34.331107679794414 19.994127598541507 34-35 20.08035860880616 25.647123349622735 34.292543208564624 19.97997483300648 36-37 20.13997846580191 25.63610912163733 34.22430009608682 19.99961231647394 38-39 20.135445194292 25.643918396170246 34.17524654587107 20.045389863666685 40-41 20.100127932992287 25.722098807916353 34.20662097335533 19.971152285736025 42-43 20.121081782468316 25.785323098912894 34.10592119580316 19.98767392281563 44-45 20.21955106095296 25.798991414711516 34.01542378821473 19.9660337361208 46-47 20.225305349099827 25.9446700728959 33.92066874093022 19.909355837074056 48-49 20.34563645132082 25.903378704525004 33.81612198742411 19.934862856730057 50-51 20.323020694114145 25.992604975354293 33.67125950654838 20.013114823983184 52-53 20.43705746299253 26.17202105914547 33.35723516111677 20.033686316745225 54-55 20.935205816319947 26.55470131425278 32.22034817683034 20.28974469259693 56-57 21.57383231609807 27.241874643040397 30.444352020854897 20.739941020006636 58-59 21.639119607845803 28.012949004063913 29.422120319948043 20.92581106814224 60-61 21.657398323960063 28.477775454415177 28.843697341097368 21.021128880527392 62-63 21.61489781628662 28.698725943105096 28.58946528570405 21.096910954904228 64-65 21.47913471556994 28.75387873069647 28.63008710345077 21.13689945028282 66-67 21.428043630017342 28.52814489761383 28.889026938317464 21.15478453405136 68-69 21.327983208977354 28.23868149300325 29.26180566264536 21.171529635374036 70-71 21.179954232429857 28.06944133751265 29.64229939556763 21.108305034489867 72-73 21.10344652250415 27.921252236890837 29.824252488687307 21.151048751917706 74-75 21.163976706231015 27.979330536446685 29.675458444090108 21.181234313232196 76-77 21.22436778557645 28.072382284497934 29.490659675997556 21.212590253928063 78-79 21.374598777617003 28.25517677911909 28.976986664301947 21.393237778961957 80-81 21.468770878462685 28.473932912555643 28.501788625953377 21.555507583028298 82-83 21.395356776107747 28.738459751394224 28.13146522694947 21.734718245548564 84-85 21.402522324992855 28.865033454164713 27.842865685333795 21.88957853550864 86-87 21.42020489187527 28.91036460440825 27.61792362230762 22.051506881408855 88-89 21.473317177507926 28.970602970727064 27.40424766429622 22.151832187468784 90-91 21.497268531040138 28.938005609833446 27.221720642404552 22.34300521672187 92-93 21.516584035005053 28.978399631485452 27.10912467384495 22.395891659664546 94-95 21.570200574836214 28.976590437849232 27.00680486460704 22.446404122707513 96-97 21.61241088385147 28.940827109072526 26.952907748615807 22.493854258460193 98-99 21.585108772135563 28.954832671281743 26.86496512449047 22.595093432092224 100 21.563038908659095 28.974936357306053 26.84428722196736 22.617737512067496 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 43706.0 1 28604.5 2 10763.5 3 7342.5 4 6527.5 5 6480.0 6 6626.0 7 6697.5 8 6811.5 9 7061.5 10 7753.0 11 11756.0 12 157183.0 13 196822.0 14 93366.5 15 100006.5 16 116500.0 17 132750.0 18 146375.5 19 159174.5 20 174972.5 21 196972.0 22 225388.0 23 254871.0 24 284428.5 25 318223.0 26 353326.0 27 394409.0 28 450765.5 29 507663.0 30 550282.5 31 595666.5 32 647213.0 33 687625.5 34 722139.5 35 770238.0 36 819941.0 37 856181.5 38 897818.5 39 929513.5 40 946772.0 41 982748.0 42 1026459.0 43 1029045.0 44 1007321.5 45 1008600.0 46 1014957.5 47 1012059.5 48 1006804.0 49 1000330.0 50 995216.5 51 992425.5 52 983396.5 53 951432.0 54 902797.0 55 849528.5 56 792961.5 57 728748.0 58 650334.0 59 555892.5 60 456009.5 61 371438.0 62 297341.5 63 227286.0 64 172536.5 65 130147.5 66 95986.5 67 72929.0 68 55029.5 69 41206.5 70 31965.0 71 26524.5 72 22692.5 73 17570.0 74 13327.5 75 11094.0 76 8316.0 77 4912.0 78 2939.0 79 2009.0 80 1356.5 81 988.5 82 705.5 83 482.0 84 344.5 85 244.0 86 149.0 87 91.5 88 63.5 89 48.5 90 37.0 91 24.5 92 15.0 93 10.5 94 8.5 95 5.0 96 3.0 97 2.5 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10694813829273751 2 0.0 3 0.0 4 0.0 5 0.009866622966032282 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.0657435576960356E-4 20-21 0.010801079273867212 22-23 0.0 24-25 9.28278084312054E-4 26-27 0.004988915494722021 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.007648640721319953 42-43 0.03931512538781868 44-45 0.015271023992667686 46-47 0.004823648015485099 48-49 0.0016264173423969934 50-51 0.0 52-53 0.0014240805220228183 54-55 7.568323815522571E-5 56-57 8.634067373218606E-4 58-59 2.7802005852940056E-5 60-61 3.1508939966665394E-4 62-63 2.9192106145587056E-4 64-65 8.649512932025794E-5 66-67 3.305349584738429E-4 68-69 5.591292288202388E-4 70-71 4.741786553806998E-4 72-73 0.0011800406928692334 74-75 0.0016464965688463389 76-77 0.0024882795238381347 78-79 0.004536360621671386 80-81 0.004281508901352768 82-83 0.007552878256715382 84-85 0.0023415467151698404 86-87 0.0027384975765145955 88-89 0.0055928368440831076 90-91 0.005622183405816767 92-93 0.005585114064679513 94-95 0.002486734967957416 96-97 0.005529510052973633 98-99 0.007909670665161446 100 0.29978902911225264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.2371765E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.227389370485366 #Duplication Level Percentage of deduplicated Percentage of total 1 51.054363146742574 15.942944770454886 2 21.38049948278392 13.353143645687087 3 11.362210344003605 10.64436499564671 4 5.815725126717252 7.2643965201485985 5 3.0065704224700474 4.694367262612842 6 1.7320024623792947 3.2451549170014604 7 1.1174956617622425 2.442753050477443 8 0.7561078461265835 1.8889019293659086 9 0.5702015660928779 1.6025315691638553 >10 2.821864967565622 15.820709111541712 >50 0.22840067731967742 4.940966788546136 >100 0.15258582453479566 8.698626428681942 >500 9.99393423725641E-4 0.19722114460016532 >1k 8.360050743263226E-4 0.6421554753973243 >5k 7.832743062595068E-5 0.1407650655403611 >10k+ 5.874557270260107E-5 8.480997325133576 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2602532 8.039512210718199 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54627 0.16874890819206181 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 50499 0.15599705484084664 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 38281 0.1182542873395998 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.014407617255345824 6.178223522875568E-6 0.0 0.0 3.398022937581562E-5 2 0.014410706367107262 1.544555880718892E-5 0.0 6.178223522875568E-6 3.706934113725341E-5 3 0.014426151925914451 1.544555880718892E-5 0.0 1.8534670568626704E-5 5.5604011705880106E-5 4 0.014438508372960202 4.015845289869119E-5 0.0 1.8534670568626704E-5 7.722779403594459E-5 5 0.014438508372960202 4.633667642156676E-5 0.0 1.8534670568626704E-5 7.722779403594459E-5 6 0.01444159748472164 4.633667642156676E-5 0.0 1.8534670568626704E-5 9.267335284313352E-5 7 0.014450864820005953 6.487134699019346E-5 0.0 2.1623782330064486E-5 9.57624646045713E-5 8 0.014457043043528829 6.796045875163124E-5 0.0 2.1623782330064486E-5 9.57624646045713E-5 9 0.014472488602336016 6.796045875163124E-5 0.0 2.1623782330064486E-5 1.1738624693463579E-4 10-11 0.014501835164069676 6.796045875163124E-5 0.0 2.316833821078338E-5 1.2201991457679246E-4 12-13 0.014532726281684054 7.568323815522571E-5 0.0 2.471289409150227E-5 1.3283180574182472E-4 14-15 0.014565161955179151 8.340601755882016E-5 0.0 2.6257449972221162E-5 1.3283180574182472E-4 16-17 0.01460686496395856 8.649512932025794E-5 0.0 3.089111761437784E-5 1.3283180574182472E-4 18-19 0.01465011252861869 1.14297135173198E-4 0.0 3.706934113725341E-5 1.3592091750326248E-4 20-21 0.01470571654032457 1.328318057418247E-4 0.0 3.706934113725341E-5 1.5908925571404587E-4 22-23 0.01495747914888175 1.4518825278757584E-4 0.0 3.706934113725341E-5 1.729902586405159E-4 24-25 0.01600314348012844 1.5445558807188918E-4 0.0 3.706934113725341E-5 1.7916848216339146E-4 26-27 0.019455225873535164 1.6372292335620255E-4 0.0 3.706934113725341E-5 1.7916848216339146E-4 28-29 0.028602085799152438 1.6526747923692144E-4 0.0 3.706934113725341E-5 1.8380214980554812E-4 30-31 0.04774840049654382 1.6681203511764034E-4 0.0 3.706934113725341E-5 2.0388137625489372E-4 32-33 0.08223678875711596 1.7607937040195368E-4 0.0 4.015845289869119E-5 2.2241604682352042E-4 34-35 0.13694650260805985 1.9152492920914262E-4 0.0 4.633667642156676E-5 2.28594270346396E-4 36-37 0.21556130782489 2.0233682037417485E-4 0.0 4.633667642156676E-5 2.3322793798855268E-4 38-39 0.32486952750336595 2.069704880163315E-4 0.0 4.633667642156676E-5 2.4095071739214713E-4 40-41 0.4638950023268734 2.069704880163315E-4 0.0 4.633667642156676E-5 2.4095071739214713E-4 42-43 0.6306514334328079 2.1469326741992598E-4 6.178223522875568E-6 4.633667642156676E-5 2.702972791258061E-4 44-45 0.8293013989197067 2.1932693506208266E-4 1.2356447045751136E-5 4.633667642156676E-5 2.888319496944328E-4 46-47 1.062141653382199 2.5639627619933607E-4 1.2356447045751136E-5 4.633667642156676E-5 2.9964384085946505E-4 48-49 1.329637108140381 2.6257449972221165E-4 1.2356447045751136E-5 4.633667642156676E-5 2.9964384085946505E-4 50-51 1.6327453878402984 2.6566361148364944E-4 1.2356447045751136E-5 4.942578818300454E-5 3.058220643823406E-4 52-53 1.9628555934469438 2.6566361148364944E-4 1.2356447045751136E-5 4.942578818300454E-5 3.1817851142809173E-4 54-55 2.3172400392749672 3.011883967401839E-4 1.2356447045751136E-5 4.942578818300454E-5 3.212676231895295E-4 56-57 2.6997817388084955 3.104557320244973E-4 1.2356447045751136E-5 5.8693123467317894E-5 3.3671318199671846E-4 58-59 3.106540529995816 3.2281217907024844E-4 1.2356447045751136E-5 5.8693123467317894E-5 3.660597437303774E-4 60-61 3.5382145520950123 3.5215874080390735E-4 1.2356447045751136E-5 5.8693123467317894E-5 3.7223796725325295E-4 62-63 3.988829462959465 3.7223796725325295E-4 1.2356447045751136E-5 6.178223522875568E-5 3.737825231339718E-4 64-65 4.4578771037044165 3.9231719370259855E-4 1.2356447045751136E-5 6.178223522875568E-5 3.9231719370259855E-4 66-67 4.945362107997509 3.9540630546403634E-4 1.2356447045751136E-5 6.178223522875568E-5 3.938617495833174E-4 68-69 5.455314222131539 3.9540630546403634E-4 1.2356447045751136E-5 6.178223522875568E-5 4.077627525097875E-4 70-71 5.984982592082947 4.0621819662906857E-4 1.2356447045751136E-5 6.796045875163124E-5 4.232083113169764E-4 72-73 6.521836235991457 4.108518642712252E-4 1.2356447045751136E-5 6.796045875163124E-5 4.2938653483985196E-4 74-75 7.070822366343015 4.2938653483985196E-4 1.2356447045751136E-5 6.796045875163124E-5 4.3247564660128975E-4 76-77 7.632809023542585 4.386538701241653E-4 1.2356447045751136E-5 6.796045875163124E-5 4.3556475836272753E-4 78-79 8.20592266130685 4.386538701241653E-4 1.2356447045751136E-5 6.796045875163124E-5 4.4637664952775977E-4 80-81 8.797465939839856 4.4483209364704085E-4 1.2356447045751136E-5 6.796045875163124E-5 4.6954498773854315E-4 82-83 9.3923948848634 4.6645587597710537E-4 1.2356447045751136E-5 7.104957051306903E-5 4.7263409949998094E-4 84-85 9.990317179183773 4.834459906650132E-4 1.2356447045751136E-5 7.413868227450682E-5 4.896242141878887E-4 86-87 10.598052037014355 4.8807965830716983E-4 1.2356447045751136E-5 7.413868227450682E-5 4.988915494722021E-4 88 11.061213375297887 4.942578818300454E-4 1.544555880718892E-5 7.413868227450682E-5 5.066143288757966E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 321310 0.0 87.23084 8 GTGGTAT 324720 0.0 86.64041 6 TGGTATC 324015 0.0 86.509865 7 AGTGGTA 325835 0.0 86.43866 5 GTATCAA 324340 0.0 86.27249 9 AAGCAGT 321590 0.0 85.78969 1 GCAGTGG 333480 0.0 84.56277 3 CAGTGGT 334325 0.0 84.06507 4 AGCAGTG 339790 0.0 82.8942 2 CAACGCA 316985 0.0 44.979183 12-13 ATCAACG 318740 0.0 44.79492 10-11 ACGCAGA 320110 0.0 44.657894 14-15 AGAGTAC 321220 0.0 44.313377 18-19 GAGTACT 313250 0.0 44.211563 20-21 TCAACGC 316790 0.0 44.128704 12-13 AACGCAG 318310 0.0 44.021507 14-15 AGTACTT 321900 0.0 43.857998 20-21 CGCAGAG 322540 0.0 43.515587 16-17 TATCAAC 324080 0.0 43.12953 10-11 GTACTTT 323005 0.0 42.82762 22-23 >>END_MODULE