FastQCFastQC Report
Thu 26 May 2016
SRR1660763_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660763_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30590052
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17435565.699748401866071No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA972610.31794977007557884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2173600.087.581178
GTGGTAT2198850.086.5376
TGGTATC2192500.086.4941257
AGTGGTA2213100.086.0034265
GTATCAA2209700.085.9632959
GCAGTGG2307850.083.28843
AAGCAGT2309950.083.115291
CAGTGGT2333600.081.522214
AGCAGTG2365850.081.292
CAACGCA2147500.045.21302412-13
ATCAACG2160500.045.03119310-11
ACGCAGA2168250.044.7417114-15
TCAACGC2145850.044.6228412-13
AACGCAG2160050.044.3774914-15
AGAGTAC2168400.044.07758718-19
GAGTACT2122050.044.04562420-21
CGCAGAG2175500.043.97661216-17
TATCAAC2203650.043.09858310-11
AGTACTT2247150.042.24965720-21
GTACTTT2220550.042.10451522-23
Produced by FastQC (version 0.11.5)