##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660763_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30590052 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60131816709563 33.0 31.0 34.0 28.0 34.0 2 31.75823633774797 34.0 31.0 34.0 28.0 34.0 3 31.801733648573073 34.0 31.0 34.0 28.0 34.0 4 35.21180487041997 37.0 35.0 37.0 32.0 37.0 5 35.20137513986573 37.0 35.0 37.0 32.0 37.0 6 35.21616759592301 37.0 35.0 37.0 32.0 37.0 7 35.18402691829357 37.0 35.0 37.0 32.0 37.0 8 35.19702392137156 37.0 35.0 37.0 32.0 37.0 9 36.80397859408673 39.0 37.0 39.0 32.0 39.0 10-11 36.73336137186037 39.0 37.0 39.0 32.0 39.0 12-13 36.778527983541835 39.0 37.0 39.0 32.0 39.0 14-15 38.05250051552707 40.0 38.0 41.0 32.0 41.0 16-17 38.01786291504179 40.0 38.0 41.0 32.0 41.0 18-19 37.9759492399686 40.0 38.0 41.0 32.0 41.0 20-21 37.93223703902171 40.0 38.0 41.0 32.0 41.0 22-23 37.79037271659428 40.0 37.5 41.0 32.0 41.0 24-25 37.73017249202453 40.0 38.0 41.0 32.0 41.0 26-27 37.718254548897136 40.0 38.0 41.0 32.0 41.0 28-29 37.643401717656445 40.0 37.5 41.0 32.0 41.0 30-31 37.53377431329636 40.0 37.0 41.0 31.0 41.0 32-33 37.41195892703942 40.0 37.0 41.0 31.0 41.0 34-35 37.2810808723045 40.0 37.0 41.0 30.0 41.0 36-37 37.07751026379425 39.5 37.0 41.0 30.0 41.0 38-39 36.61478682677624 39.0 35.0 41.0 30.0 41.0 40-41 36.600044060075476 39.0 35.0 41.0 30.0 41.0 42-43 36.61243231950047 39.0 35.0 41.0 30.0 41.0 44-45 36.55119528727836 39.0 35.0 41.0 30.0 41.0 46-47 36.664754051415144 39.5 35.0 41.0 30.0 41.0 48-49 36.59995303375097 39.5 35.0 41.0 29.5 41.0 50-51 35.486246476468885 38.0 34.0 40.0 27.5 40.5 52-53 35.50978234688846 38.5 34.5 39.5 27.0 40.5 54-55 35.73326066918749 39.0 35.0 41.0 25.5 41.0 56-57 35.36292788910591 39.0 35.0 41.0 23.0 41.0 58-59 35.0626819627505 39.0 34.0 41.0 22.0 41.0 60-61 34.85079123108388 38.5 34.0 41.0 22.0 41.0 62-63 34.61390356577361 38.0 34.0 40.5 22.0 41.0 64-65 34.35499756587534 37.5 34.0 40.0 21.5 41.0 66-67 34.09090942375646 37.0 33.0 40.0 21.0 41.0 68-69 33.817607060622194 36.5 33.0 39.5 21.0 41.0 70-71 33.51651363652471 36.0 33.0 39.0 20.5 41.0 72-73 33.18873356279355 35.0 32.5 39.0 20.0 41.0 74-75 32.83554140411399 35.0 32.0 38.5 20.0 40.0 76-77 32.450199316431366 35.0 32.0 37.0 19.5 39.5 78-79 32.09022093195526 35.0 32.0 37.0 19.0 39.0 80-81 31.755950333134443 35.0 31.0 36.0 18.0 39.0 82-83 31.441530893768995 35.0 31.0 36.0 18.0 37.5 84-85 31.175539126249276 35.0 31.0 35.5 17.0 37.0 86-87 30.92161379784513 35.0 31.0 35.0 17.0 37.0 88-89 30.727192944948246 35.0 31.0 35.0 15.5 36.0 90-91 30.551774184627078 35.0 30.0 35.0 15.0 36.0 92-93 30.387628026915415 34.0 30.0 35.0 15.0 36.0 94-95 30.226211645537575 34.0 30.0 35.0 14.0 35.5 96-97 30.110842407198263 34.0 30.0 35.0 13.0 35.0 98-99 30.00753627355717 34.0 29.5 35.0 11.5 35.0 100 29.887237360694908 34.0 29.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7275899126969989 1101 2 0.710005852532877 1101 3 0.7079140821718575 1101 4 0.6272784188075207 1101 5 0.6341715995501858 1101 6 0.656934528312739 1101 7 0.5906110057315956 1101 8 0.6608754280790166 1101 9 0.8090298176831183 1101 10-11 0.7602780430136278 1101 12-13 0.743306855631964 1101 14-15 0.9018364007752595 1101 16-17 0.900955904367045 1101 18-19 0.8749392428082601 1101 20-21 0.8011314100735731 1101 22-23 0.7785488422521354 1101 24-25 0.7522624465008647 1101 26-27 0.757930039288425 1101 28-29 0.7153825340067215 1101 30-31 0.7729935887939874 1101 32-33 0.7872372449215277 1101 34-35 0.7581460099346629 1101 36-37 0.7671383462665986 1101 38-39 0.8005177739392622 1101 40-41 0.7887669598310012 1101 42-43 0.747134771981969 1101 44-45 0.7010490502450395 1101 46-47 0.7769666818391485 1101 48-49 0.7747772689116985 1101 50-51 0.7876739967206774 1101 52-53 0.8238913913931256 1101 54-55 0.8756814028665616 1101 56-57 0.7967491405227065 1101 58-59 0.8075828569314183 1101 60-61 0.7381016000007321 1101 62-63 0.72720685729184 1101 64-65 0.6754117280171243 1101 66-67 0.6268016674267685 1101 68-69 0.6164437373875202 1101 70-71 0.6499298579206823 1101 72-73 0.6075678726129823 1101 74-75 0.5494551221338995 1101 76-77 0.5063235722202819 1101 78-79 0.47718552819439 1101 80-81 0.4614368496919603 1101 82-83 0.4442936866711449 1101 84-85 0.3416450759635481 1101 86-87 0.298868014709047 1101 88-89 0.36385211176072474 1101 90-91 0.3258396238616186 1101 92-93 0.30083209762691254 1101 94-95 0.26440378056881286 1101 96-97 0.24967472004566815 1101 98-99 0.2379957643268824 1101 100 0.20031288044256712 1102 1 0.6305376324335654 1102 2 0.6299470802726255 1102 3 0.594654636401426 1102 4 0.5543606726583192 1102 5 0.5341861459596089 1102 6 0.5669482338446556 1102 7 0.5725298801291032 1102 8 0.5907956981789084 1102 9 0.723409352808531 1102 10-11 0.6652440615869679 1102 12-13 0.6555470035170359 1102 14-15 0.8184308728875536 1102 16-17 0.8142653389751828 1102 18-19 0.8143845070334379 1102 20-21 0.7527187758845173 1102 22-23 0.7238851741990757 1102 24-25 0.7721805819127994 1102 26-27 0.7460068728064684 1102 28-29 0.7318578400254268 1102 30-31 0.7511638306607509 1102 32-33 0.7493342490692143 1102 34-35 0.7189239644935768 1102 36-37 0.6806808879419108 1102 38-39 0.7920667226486913 1102 40-41 0.7079809455291652 1102 42-43 0.707524659674803 1102 44-45 0.6381187117710709 1102 46-47 0.7104638818086002 1102 48-49 0.73298170112934 1102 50-51 0.6789975549451768 1102 52-53 0.7471842373785407 1102 54-55 0.7885223431336215 1102 56-57 0.7391142630779868 1102 58-59 0.7415199000737545 1102 60-61 0.7345561138151027 1102 62-63 0.7121571676080478 1102 64-65 0.6563211788533749 1102 66-67 0.6277096631615748 1102 68-69 0.5962303364296346 1102 70-71 0.5749188021199458 1102 72-73 0.5720898302137982 1102 74-75 0.5143848863267664 1102 76-77 0.5168326183796239 1102 78-79 0.46781721787412067 1102 80-81 0.4698117770469743 1102 82-83 0.4697984178072687 1102 84-85 0.3769278451707123 1102 86-87 0.33636592453153114 1102 88-89 0.3729116242383945 1102 90-91 0.30167125648739557 1102 92-93 0.28506128338896275 1102 94-95 0.2882377432852543 1102 96-97 0.23357064449121623 1102 98-99 0.22875086722808646 1102 100 0.24292060764053858 1103 1 0.5818219300150034 1103 2 0.6001337891304139 1103 3 0.575178955183457 1103 4 0.5095448710240689 1103 5 0.5184196138420347 1103 6 0.5532500228356838 1103 7 0.5460514409818629 1103 8 0.5498996625061565 1103 9 0.6504335564767416 1103 10-11 0.635078797515817 1103 12-13 0.6092682462684778 1103 14-15 0.7504227659852063 1103 16-17 0.7376587761012772 1103 18-19 0.7138135185534082 1103 20-21 0.66557263047509 1103 22-23 0.6871409251862346 1103 24-25 0.6626182866448929 1103 26-27 0.6980309088001775 1103 28-29 0.6618563941474491 1103 30-31 0.6889298636048693 1103 32-33 0.6911849927501876 1103 34-35 0.6558900424520075 1103 36-37 0.650002225630459 1103 38-39 0.6810945174490044 1103 40-41 0.6111833122197439 1103 42-43 0.6250805855155193 1103 44-45 0.6071462259121532 1103 46-47 0.671185061612384 1103 48-49 0.6670055928889695 1103 50-51 0.6099075815416839 1103 52-53 0.669793712877599 1103 54-55 0.7879552407567658 1103 56-57 0.7719329173146718 1103 58-59 0.8215546718883502 1103 60-61 0.7536670597957311 1103 62-63 0.7222840742831167 1103 64-65 0.7145377961080968 1103 66-67 0.663744475571761 1103 68-69 0.6843785407187042 1103 70-71 0.6477493834895611 1103 72-73 0.6238176088289222 1103 74-75 0.6006196885037056 1103 76-77 0.6079788203290377 1103 78-79 0.5749484056295486 1103 80-81 0.5342951387094779 1103 82-83 0.469991831876591 1103 84-85 0.47796198871948903 1103 86-87 0.4519532537591324 1103 88-89 0.4684168483296851 1103 90-91 0.43962733028436674 1103 92-93 0.4046374230609935 1103 94-95 0.41528019592530185 1103 96-97 0.37047451078771587 1103 98-99 0.3105894854236624 1103 100 0.3314360537817116 1104 1 0.5510878447915104 1104 2 0.531527996166222 1104 3 0.5231178159318226 1104 4 0.4748997558307124 1104 5 0.4853692240973544 1104 6 0.4844082236258629 1104 7 0.46296467310246925 1104 8 0.5025375670158283 1104 9 0.5995038146920066 1104 10-11 0.5759779143357591 1104 12-13 0.5722509844973729 1104 14-15 0.6700432783290893 1104 16-17 0.6753935846697132 1104 18-19 0.6954985143710957 1104 20-21 0.6193382787581001 1104 22-23 0.6042432080002627 1104 24-25 0.6705436637902977 1104 26-27 0.675567775851448 1104 28-29 0.6567345255540289 1104 30-31 0.6393055817062034 1104 32-33 0.6396376115985802 1104 34-35 0.6610494986086124 1104 36-37 0.6241712917380013 1104 38-39 0.6469551771114368 1104 40-41 0.6370521487365934 1104 42-43 0.6388946000118381 1104 44-45 0.5999120701683012 1104 46-47 0.6604363994727507 1104 48-49 0.6996984173930443 1104 50-51 0.6463659463440834 1104 52-53 0.6743441659012959 1104 54-55 0.7391126167980318 1104 56-57 0.723206470902376 1104 58-59 0.7458669892084373 1104 60-61 0.7700567770824307 1104 62-63 0.70694858860751 1104 64-65 0.6734406900036447 1104 66-67 0.6733582720304909 1104 68-69 0.6826907299308331 1104 70-71 0.7177966335223473 1104 72-73 0.6564544515315518 1104 74-75 0.6399590656424508 1104 76-77 0.6418101565851302 1104 78-79 0.5751573407498896 1104 80-81 0.5509030790698155 1104 82-83 0.5665245694156482 1104 84-85 0.5114354802452148 1104 86-87 0.5245903754433634 1104 88-89 0.5304677790903405 1104 90-91 0.44881880626299875 1104 92-93 0.44250220199614176 1104 94-95 0.43321583720556944 1104 96-97 0.49189285379804204 1104 98-99 0.4397279355863084 1104 100 0.3992591432601351 1105 1 0.4133035745667186 1105 2 0.40779473324023385 1105 3 0.3996117451728445 1105 4 0.3817941073389477 1105 5 0.3986868220082087 1105 6 0.4152291917349302 1105 7 0.3739304116197246 1105 8 0.4081855668276262 1105 9 0.48006510031051874 1105 10-11 0.48493486628320426 1105 12-13 0.48495724565829335 1105 14-15 0.5849415504801243 1105 16-17 0.5380677188283229 1105 18-19 0.542821842513689 1105 20-21 0.5052413634126438 1105 22-23 0.508530850854811 1105 24-25 0.49278667642597185 1105 26-27 0.4586084416737748 1105 28-29 0.45704751279394884 1105 30-31 0.5197833281356168 1105 32-33 0.4949607427434657 1105 34-35 0.5046744989125571 1105 36-37 0.4811242313210826 1105 38-39 0.5007998766054413 1105 40-41 0.45078698026962627 1105 42-43 0.4596604802521256 1105 44-45 0.4412451308610059 1105 46-47 0.46164238206625186 1105 48-49 0.47177614638680154 1105 50-51 0.4302248538848161 1105 52-53 0.45136337861435294 1105 54-55 0.5578192805423896 1105 56-57 0.5872563437923901 1105 58-59 0.6216957078645109 1105 60-61 0.6406538618108186 1105 62-63 0.6018435477863662 1105 64-65 0.5742467655170742 1105 66-67 0.5750254541116178 1105 68-69 0.6007249933277734 1105 70-71 0.5935509689192529 1105 72-73 0.5407891196278811 1105 74-75 0.5403162901217371 1105 76-77 0.5409636308387817 1105 78-79 0.4932596804317839 1105 80-81 0.5083243564030973 1105 82-83 0.4976974582196476 1105 84-85 0.4586337967280265 1105 86-87 0.44830764823791824 1105 88-89 0.455221001793074 1105 90-91 0.39953106447689635 1105 92-93 0.4242839426210381 1105 94-95 0.4449680723377334 1105 96-97 0.41404638500258883 1105 98-99 0.4116004320649935 1105 100 0.4251410559055131 1106 1 0.3624530713842944 1106 2 0.3310971002604042 1106 3 0.30560386440977183 1106 4 0.3212000806641768 1106 5 0.30927086610228116 1106 6 0.339300315010135 1106 7 0.29778769657147564 1106 8 0.350900110658074 1106 9 0.43158392291201153 1106 10-11 0.3989633496483762 1106 12-13 0.40186894564416775 1106 14-15 0.42039398078352264 1106 16-17 0.4344117777590739 1106 18-19 0.4163550945193535 1106 20-21 0.4145269729489627 1106 22-23 0.38084288417768875 1106 24-25 0.4328343280194673 1106 26-27 0.4291710748903128 1106 28-29 0.42952993015981633 1106 30-31 0.43712400951314834 1106 32-33 0.3921062775848583 1106 34-35 0.37542623666487884 1106 36-37 0.36956157154651237 1106 38-39 0.38117412864673383 1106 40-41 0.37865798280837026 1106 42-43 0.349084544472106 1106 44-45 0.30724708930415545 1106 46-47 0.3436561717577362 1106 48-49 0.38353370378563767 1106 50-51 0.3647088762101376 1106 52-53 0.3757000461164566 1106 54-55 0.40447019730344635 1106 56-57 0.3732857659708273 1106 58-59 0.4370094599354388 1106 60-61 0.41172487218436515 1106 62-63 0.39154587118644457 1106 64-65 0.3657595721584954 1106 66-67 0.3676911346235556 1106 68-69 0.32332491363447247 1106 70-71 0.37702266159357833 1106 72-73 0.3535561645296852 1106 74-75 0.34501181597103425 1106 76-77 0.3463633758664315 1106 78-79 0.2999141038267723 1106 80-81 0.2639916142984511 1106 82-83 0.292902351127978 1106 84-85 0.2880377646676955 1106 86-87 0.27303390804040006 1106 88-89 0.22643274819291292 1106 90-91 0.2772358541143305 1106 92-93 0.24725672216356998 1106 94-95 0.27396322644914406 1106 96-97 0.20437942714282187 1106 98-99 0.21581490579313112 1106 100 0.25723042882068015 1107 1 0.4802682080666969 1107 2 0.4861492352253869 1107 3 0.3968679141549707 1107 4 0.45874268734578294 1107 5 0.4591939765005293 1107 6 0.49345945377229583 1107 7 0.48542697826875525 1107 8 0.49259661903050755 1107 9 0.6128541001417176 1107 10-11 0.5668836473468772 1107 12-13 0.5027226957213742 1107 14-15 0.6239707894699293 1107 16-17 0.6606824362546888 1107 18-19 0.6833906349277044 1107 20-21 0.6287497175929744 1107 22-23 0.605318153530753 1107 24-25 0.6435133212444129 1107 26-27 0.6380289147755605 1107 28-29 0.6556722039708163 1107 30-31 0.6681571121625254 1107 32-33 0.636947997290541 1107 34-35 0.6360097591324987 1107 36-37 0.598386755067942 1107 38-39 0.6631195487961605 1107 40-41 0.6673236064394885 1107 42-43 0.6415342087108158 1107 44-45 0.6052417676599404 1107 46-47 0.6455467542867623 1107 48-49 0.633927196910733 1107 50-51 0.596164546574343 1107 52-53 0.6356254257515275 1107 54-55 0.7280155864106703 1107 56-57 0.7480458040081217 1107 58-59 0.7941464702749528 1107 60-61 0.7864156645255491 1107 62-63 0.7485316330932008 1107 64-65 0.6918568124050495 1107 66-67 0.6556550537289851 1107 68-69 0.7186778994994398 1107 70-71 0.7205775686150204 1107 72-73 0.7026476961251049 1107 74-75 0.715016528628631 1107 76-77 0.7084080210488608 1107 78-79 0.7343185745434013 1107 80-81 0.7249987962430957 1107 82-83 0.6015569938117409 1107 84-85 0.6174312474270032 1107 86-87 0.643714155569338 1107 88-89 0.6783587135078157 1107 90-91 0.668613103919764 1107 92-93 0.6848221153153133 1107 94-95 0.7013882882068891 1107 96-97 0.6821005030495044 1107 98-99 0.6715936667131999 1107 100 0.6461486429761472 1108 1 0.2685647738294712 1108 2 0.2133340907036434 1108 3 0.24853544357808133 1108 4 0.26105211789957394 1108 5 0.2593756085962795 1108 6 0.262273306550739 1108 7 0.25695785929876536 1108 8 0.2711366955774892 1108 9 0.3198096896110201 1108 10-11 0.28429140737005554 1108 12-13 0.26119708592608504 1108 14-15 0.3233046710815515 1108 16-17 0.3309582961183324 1108 18-19 0.3213749690302876 1108 20-21 0.2651061284336791 1108 22-23 0.2559309710504536 1108 24-25 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2316 84-85 -1.570322730701644 2316 86-87 -1.465737024744378 2316 88-89 -1.6515432980314806 2316 90-91 -1.5466064133204753 2316 92-93 -1.4617653245065299 2316 94-95 -1.4565847401371208 2316 96-97 -1.3311805557459948 2316 98-99 -1.369855088603117 2316 100 -1.4754736989411157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 36.0 4 95.0 5 204.0 6 1689.0 7 19774.0 8 29469.0 9 38257.0 10 42489.0 11 45910.0 12 52058.0 13 60517.0 14 72863.0 15 88098.0 16 103533.0 17 120361.0 18 133226.0 19 148290.0 20 170261.0 21 208202.0 22 269332.0 23 374154.0 24 525979.0 25 624585.0 26 599194.0 27 508975.0 28 463265.0 29 483818.0 30 548154.0 31 657184.0 32 803907.0 33 1025298.0 34 1385927.0 35 2021826.0 36 3291182.0 37 5851267.0 38 7394104.0 39 2420072.0 40 6497.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.745201654701635 35.344690533281025 27.65442592786706 19.25568188415028 2 21.150456600484198 33.90647545923 26.28583271721876 18.657235223067044 3 26.528332539624188 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23.653537086629107 26.69041371914082 29.368611322176392 20.287437872053676 24-25 20.38429961694104 29.923687908655506 25.93441244114047 23.75760003326298 26-27 20.4043974125569 26.58359860128259 32.622700117028515 20.389303869131997 28-29 20.377563361936925 26.679050571915415 32.62372734312579 20.319658723021863 30-31 20.39851658437032 26.652534040287307 32.5855376279624 20.36341174737997 32-33 20.415260228871386 26.6642767307115 32.550419200761816 20.3700438396553 34-35 20.447457372155302 26.703711288709247 32.52008157469075 20.3287497644447 36-37 20.47235503367208 26.68383670879861 32.46147936561099 20.38232889191832 38-39 20.50689175726483 26.70416210740622 32.400158001308306 20.38878813402064 40-41 20.495073171940422 26.797209707715403 32.3996126020618 20.308104518282374 42-43 20.55274018292345 26.828280094858403 32.32111809940871 20.297861622809435 44-45 20.595123667705174 26.855578270841722 32.24624816924321 20.303049892209895 46-47 20.626355138728904 26.955676476210282 32.17438523150233 20.243583153558482 48-49 20.713021770168247 26.95972937191452 32.05605407813642 20.271194779780814 50-51 20.778871595898206 27.01547749588461 31.947424726048983 20.258226182168197 52-53 20.90726268002424 27.198958834827856 31.65472192066925 20.23905656447866 54-55 21.365500257126353 27.43399117011775 30.805247663287744 20.39526090946816 56-57 21.877915800748575 27.95671474475415 29.61091777169663 20.554451682800643 58-59 22.07121538141181 28.50331638453137 28.883219394315173 20.542248839741642 60-61 22.20988720565821 28.790285185833724 28.46966088822357 20.53016672028449 62-63 22.280092237595973 28.95585899553346 28.292694138211296 20.47135462865927 64-65 22.41000289212063 28.974588200066027 28.27382450841108 20.34158439940227 66-67 22.530157724944402 28.770693316225422 28.437141389675357 20.262007569154814 68-69 22.654505323642997 28.546941407195003 28.63933679935709 20.15921646980491 70-71 22.718334146523297 28.416120225196252 28.82721600097514 20.038329627305313 72-73 22.8825041452061 28.31737298226857 28.84387560445454 19.95624726807079 74-75 23.091609376145215 28.34996528113126 28.700844820900123 19.857580521823404 76-77 23.401020896495588 28.453878985419838 28.399835929685125 19.745264188399453 78-79 23.676475958023595 28.536290869919256 28.052509736657417 19.734723435399733 80-81 23.9447738857423 28.626128160184066 27.73423699794555 19.694860956128085 82-83 24.089883582979336 28.764868699910217 27.47025957508243 19.674988142028017 84-85 24.256332782240086 28.780131914058497 27.29612778268252 19.667407521018895 86-87 24.436641658754336 28.75562238112893 27.12638025420418 19.68135570591255 88-89 24.611776193412325 28.764693272891655 26.979643308596383 19.643887225099636 90-91 24.780119542696468 28.693905578045886 26.83803547874784 19.6879394005098 92-93 24.90166278005768 28.687627350236255 26.77236025583164 19.63834961387443 94-95 25.023906721902637 28.72352937886482 26.67729976254035 19.575264136692187 96-97 25.092948269050886 28.70500451731695 26.625952811749325 19.576094401882834 98-99 25.154137113032533 28.73518630655767 26.534590701596816 19.576085878812982 100 25.18479786324757 28.802562831273505 26.445326897407696 19.56731240807123 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 57453.0 1 35594.0 2 11821.0 3 9413.5 4 8868.0 5 9043.0 6 9435.5 7 9704.0 8 9925.0 9 10144.5 10 10901.0 11 13306.5 12 97128.0 13 123861.5 14 69312.5 15 75829.5 16 87700.5 17 99377.0 18 110643.0 19 121228.0 20 134517.5 21 153306.0 22 177020.5 23 203224.0 24 228760.0 25 259462.0 26 297144.5 27 342164.0 28 398473.5 29 454314.0 30 500361.0 31 544742.5 32 594255.5 33 640440.5 34 681103.5 35 734188.0 36 796752.0 37 845618.5 38 885687.0 39 914790.5 40 931585.5 41 954026.5 42 986799.0 43 1007472.0 44 1020749.5 45 1042136.5 46 1057469.5 47 1067204.5 48 1058557.5 49 1034593.0 50 1013949.5 51 990317.5 52 956970.5 53 909081.0 54 847401.0 55 781437.5 56 714247.5 57 645177.5 58 570158.0 59 488555.0 60 404900.0 61 328167.0 62 258730.5 63 195350.5 64 144671.5 65 106092.0 66 77166.0 67 57093.0 68 42908.0 69 32202.0 70 24716.5 71 19842.5 72 16247.0 73 12998.5 74 10747.0 75 9266.0 76 7406.5 77 5237.0 78 3703.0 79 2880.5 80 2295.0 81 1875.0 82 1550.0 83 1255.0 84 1026.0 85 895.0 86 772.5 87 674.5 88 559.0 89 450.0 90 396.5 91 350.0 92 295.5 93 233.0 94 201.5 95 201.5 96 161.0 97 125.0 98 108.0 99 68.0 100 41.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04624706097263254 2 0.01491007599464035 3 0.005233727618377373 4 1.896041235889367E-4 5 6.309240664252549E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.2690366136023566E-6 12-13 3.2690366136023566E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.03784236783906088 22-23 0.07482824808535794 24-25 0.031093441750278818 26-27 0.035147047151145736 28-29 0.039852825356426334 30-31 0.03341118870932289 32-33 0.03410258995309979 34-35 0.03502609279644245 36-37 0.03283910730194248 38-39 0.04279168927205485 40-41 0.06348305651785097 42-43 0.07180438921777577 44-45 0.07445230887479368 46-47 0.04752688880685786 48-49 0.030044080997312463 50-51 0.027754120849484008 52-53 0.027933917863232138 54-55 0.0287135830955763 56-57 0.028865593298108807 58-59 0.03659523037097158 60-61 0.056219257162426525 62-63 0.07984458476893076 64-65 0.10751697970307471 66-67 0.08889981618860929 68-69 0.05235852492176215 70-71 0.04565373082726371 72-73 0.049936168791082806 74-75 0.04726700039607648 76-77 0.05019932623847779 78-79 0.04317907011076673 80-81 0.041773384366917715 82-83 0.04093324195722191 84-85 0.0482722291547592 86-87 0.052070849699765144 88-89 0.05809241514202068 90-91 0.05489202829730397 92-93 0.05038566132545313 94-95 0.05235362136684174 96-97 0.054246393566117504 98-99 0.04877566079325397 100 0.05043796591127077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0590052E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.19359260920958 #Duplication Level Percentage of deduplicated Percentage of total 1 58.919003165419426 20.73571394344501 2 19.04777721419179 13.407194227745014 3 9.145502678906988 9.65589286463662 4 4.343069022209506 6.113928073652781 5 2.237725553091695 3.9376800743363645 6 1.315001461503718 2.776777543000622 7 0.9021148321607195 2.2224063322852885 8 0.6593637312414896 1.856430283088107 9 0.4601430119459438 1.4574677133960183 >10 2.6126241908422223 16.674388820296013 >50 0.2090871792213237 5.20042702545174 >100 0.14605051209814426 8.9153849530549 >500 0.0018608242296436405 0.3958157895263452 >1k 6.027267394602299E-4 0.46510770804218027 >5k 3.6948098981987475E-5 0.08348809490693367 >10k+ 3.6948098950170214E-5 6.101896553136091 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1743556 5.699748401866071 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97261 0.31794977007557884 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00821181997336912 9.80710984080707E-6 0.0 3.2690366136023566E-6 7.845687872645656E-5 2 0.008228165156437131 9.80710984080707E-6 0.0 6.538073227204713E-6 7.845687872645656E-5 3 0.008228165156437131 1.3076146454409426E-5 0.0 6.538073227204713E-6 7.845687872645656E-5 4 0.008231434193050734 1.6345183068011783E-5 0.0 6.538073227204713E-6 7.845687872645656E-5 5 0.008231434193050734 2.2883256295216496E-5 0.0 9.80710984080707E-6 7.845687872645656E-5 6 0.008241241302891542 2.2883256295216496E-5 0.0 9.80710984080707E-6 8.172591534005892E-5 7 0.008247779376118746 2.2883256295216496E-5 0.0 1.3076146454409426E-5 8.499495195366128E-5 8 0.008247779376118746 3.2690366136023566E-5 0.0 1.3076146454409426E-5 8.499495195366128E-5 9 0.008247779376118746 3.5959402749625926E-5 0.0 1.3076146454409426E-5 8.499495195366128E-5 10-11 0.00825268293103915 5.557362243124006E-5 0.0 2.2883256295216496E-5 8.499495195366128E-5 12-13 0.008264124559186757 5.884265904484242E-5 0.0 2.2883256295216496E-5 8.499495195366128E-5 14-15 0.008273931669027564 6.0477177351643595E-5 0.0 2.6152292908818853E-5 8.826398856726363E-5 16-17 0.008301718480243185 6.538073227204713E-5 0.0 3.2690366136023566E-5 8.826398856726363E-5 18-19 0.008309891071777192 6.538073227204713E-5 0.0 4.249747597683064E-5 8.826398856726363E-5 20-21 0.00834911951114042 7.028428719245067E-5 0.0 4.249747597683064E-5 1.0134013502167306E-4 22-23 0.008569779482558578 9.480206179446834E-5 3.2690366136023566E-6 5.067006751083653E-5 1.1768531808968484E-4 24-25 0.009338003086755132 1.0460917163527541E-4 6.538073227204713E-6 5.5573622431240065E-5 1.2422339131688956E-4 26-27 0.012185333977202785 1.1768531808968485E-4 9.80710984080707E-6 6.0477177351643595E-5 1.2749242793049192E-4 28-29 0.02063906265997848 1.438376109985037E-4 9.80710984080707E-6 6.538073227204713E-5 1.2749242793049192E-4 30-31 0.04007021629123089 1.65086348986919E-4 9.80710984080707E-6 6.701525057884831E-5 1.2749242793049192E-4 32-33 0.07752193425496628 1.6998990390732253E-4 9.80710984080707E-6 6.86497688856495E-5 1.3403050115769663E-4 34-35 0.1389879951822246 1.732589405209249E-4 9.80710984080707E-6 6.86497688856495E-5 1.3729953777129897E-4 36-37 0.2306664924923959 1.7979701374812963E-4 9.80710984080707E-6 6.86497688856495E-5 1.4056857438490134E-4 38-39 0.36027562163019533 1.83066050361732E-4 9.80710984080707E-6 6.86497688856495E-5 1.4547212930530486E-4 40-41 0.5296869060569103 1.8960412358893667E-4 9.80710984080707E-6 6.86497688856495E-5 1.503756842257084E-4 42-43 0.7398401937989514 1.9450767850934022E-4 9.80710984080707E-6 6.86497688856495E-5 1.6672086729372022E-4 44-45 0.991438327728243 2.0921834327055082E-4 9.80710984080707E-6 6.86497688856495E-5 1.7816249544132845E-4 46-47 1.2884809741415282 2.124873798841532E-4 9.80710984080707E-6 6.86497688856495E-5 1.8633508697533433E-4 48-49 1.6308733309770118 2.271980446453638E-4 9.80710984080707E-6 7.028428719245067E-5 1.896041235889367E-4 50-51 2.0198053275620453 2.2883256295216497E-4 9.80710984080707E-6 8.009139703325774E-5 1.896041235889367E-4 52-53 2.444884696501987 2.353706361793697E-4 9.80710984080707E-6 8.989850687406481E-5 1.9287316020253904E-4 54-55 2.9050179450495865 2.419087094065744E-4 9.80710984080707E-6 9.153302518086598E-5 2.0104575173654493E-4 56-57 3.403067114760053 2.5335033755418264E-4 9.80710984080707E-6 9.153302518086598E-5 2.1085286157735203E-4 58-59 3.9380662053140676 2.6806100231539326E-4 9.80710984080707E-6 9.316754348766717E-5 2.1575641649775555E-4 60-61 4.5062966875636565 2.713300389289956E-4 9.80710984080707E-6 9.480206179446835E-5 2.2229448972496026E-4 62-63 5.095522557464106 2.795026304630015E-4 9.80710984080707E-6 9.480206179446835E-5 2.4027419109977322E-4 64-65 5.709995197131407 2.876752219970074E-4 9.80710984080707E-6 1.0134013502167306E-4 2.5498485586098385E-4 66-67 6.347620788614547 3.072894416786215E-4 9.80710984080707E-6 1.0134013502167306E-4 2.5498485586098385E-4 68-69 7.008845555411282 3.2036558813303096E-4 9.80710984080707E-6 1.0134013502167306E-4 2.6642648400859205E-4 70-71 7.693457010141729 3.2363462474663333E-4 9.80710984080707E-6 1.0134013502167306E-4 2.778681121562003E-4 72-73 8.391679098812908 3.2853817966703685E-4 9.80710984080707E-6 1.0460917163527541E-4 2.8440618538340504E-4 74-75 9.10377824790883 3.3671077120104274E-4 9.80710984080707E-6 1.0460917163527541E-4 2.9094425861060977E-4 76-77 9.832044417577322 3.432488444282475E-4 9.80710984080707E-6 1.1441628147608248E-4 2.9094425861060977E-4 78-79 10.577659364554203 3.4488336273504863E-4 1.3076146454409426E-5 1.1768531808968484E-4 2.9094425861060977E-4 80-81 11.335469452618124 3.465178810418498E-4 1.3076146454409426E-5 1.1768531808968484E-4 2.991168501446156E-4 82-83 12.101087634633638 3.612285458030604E-4 1.3076146454409426E-5 1.1768531808968484E-4 3.0728944167862155E-4 84-85 12.868044487142422 3.7267017395066867E-4 1.3076146454409426E-5 1.1768531808968484E-4 3.1055847829222386E-4 86-87 13.644622114405035 3.857463204050781E-4 1.3076146454409426E-5 1.2585790962369074E-4 3.1382751490582623E-4 88 14.233346187185298 3.857463204050781E-4 1.3076146454409426E-5 1.3403050115769663E-4 3.1382751490582623E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 217360 0.0 87.58117 8 GTGGTAT 219885 0.0 86.537 6 TGGTATC 219250 0.0 86.494125 7 AGTGGTA 221310 0.0 86.003426 5 GTATCAA 220970 0.0 85.963295 9 GCAGTGG 230785 0.0 83.2884 3 AAGCAGT 230995 0.0 83.11529 1 CAGTGGT 233360 0.0 81.52221 4 AGCAGTG 236585 0.0 81.29 2 CAACGCA 214750 0.0 45.213024 12-13 ATCAACG 216050 0.0 45.031193 10-11 ACGCAGA 216825 0.0 44.74171 14-15 TCAACGC 214585 0.0 44.62284 12-13 AACGCAG 216005 0.0 44.37749 14-15 AGAGTAC 216840 0.0 44.077587 18-19 GAGTACT 212205 0.0 44.045624 20-21 CGCAGAG 217550 0.0 43.976612 16-17 TATCAAC 220365 0.0 43.098583 10-11 AGTACTT 224715 0.0 42.249657 20-21 GTACTTT 222055 0.0 42.104515 22-23 >>END_MODULE