FastQCFastQC Report
Thu 26 May 2016
SRR1660763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30590052
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19032966.221944310522911No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730530.23881293173349297No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT366490.11980692285191276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2346150.087.6360868
GTGGTAT2377400.086.80046
TGGTATC2379400.086.458847
GTATCAA2380500.086.253079
AGTGGTA2394700.086.22165
GCAGTGG2463700.083.989953
AAGCAGT2424400.083.417371
CAGTGGT2505150.082.245694
AGCAGTG2538200.081.321042
CAACGCA2301750.045.32785812-13
ATCAACG2317400.045.07747710-11
ACGCAGA2332550.044.85407614-15
TCAACGC2300200.044.64029312-13
AACGCAG2315800.044.40014314-15
AGAGTAC2363700.044.06925218-19
GAGTACT2316150.043.98880420-21
CGCAGAG2344700.043.9831816-17
TATCAAC2370150.043.2960310-11
AGTACTT2444250.042.4189420-21
GTACTTT2413300.042.22670722-23
Produced by FastQC (version 0.11.5)