FastQCFastQC Report
Thu 26 May 2016
SRR1660762_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660762_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30781422
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14228504.622431023492027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1128970.36676993025208515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1822900.085.1716168
AGTGGTA1837650.084.53445
GTGGTAT1849100.084.023246
TGGTATC1843900.083.997817
GTATCAA1848650.083.835349
AAGCAGT1949600.079.966231
GCAGTGG1958550.079.83013
CAGTGGT1960650.079.178484
AGCAGTG2026500.077.172452
CAACGCA1787200.044.19339812-13
ATCAACG1800200.043.97602510-11
ACGCAGA1811600.043.6088514-15
TCAACGC1788750.043.46448512-13
GAGTACT1752000.043.3054120-21
AACGCAG1798450.043.29543314-15
AGAGTAC1818550.042.91510818-19
CGCAGAG1824100.042.65453716-17
AGTACTT1842500.041.88913320-21
TATCAAC1852000.041.8126810-11
GTACTTT1835450.041.3445122-23
Produced by FastQC (version 0.11.5)