##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660762_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30781422 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.683204011822454 33.0 31.0 34.0 30.0 34.0 2 31.835602169386455 34.0 31.0 34.0 30.0 34.0 3 31.87629190750187 34.0 31.0 34.0 30.0 34.0 4 35.29569027057944 37.0 35.0 37.0 33.0 37.0 5 35.289560891631325 37.0 35.0 37.0 33.0 37.0 6 35.298229139641435 37.0 35.0 37.0 32.0 37.0 7 35.285549640949014 37.0 35.0 37.0 32.0 37.0 8 35.284593252384504 37.0 35.0 37.0 32.0 37.0 9 36.89656725410541 39.0 37.0 39.0 33.0 39.0 10-11 36.84767022784068 39.0 37.0 39.0 33.0 39.0 12-13 36.86469086775783 39.0 37.0 39.0 33.0 39.0 14-15 38.14600702982468 40.0 38.0 41.0 33.0 41.0 16-17 38.11466185675243 40.0 38.0 41.0 33.0 41.0 18-19 38.06703437872363 40.0 38.0 41.0 33.0 41.0 20-21 38.027586623515965 40.0 38.0 41.0 33.0 41.0 22-23 37.90443193300166 40.0 37.5 41.0 32.5 41.0 24-25 37.82402741172906 40.0 38.0 41.0 32.0 41.0 26-27 37.78507500400728 40.0 38.0 41.0 32.0 41.0 28-29 37.68975213360838 40.0 38.0 41.0 32.0 41.0 30-31 37.56537311694048 40.0 37.0 41.0 31.5 41.0 32-33 37.43136449316734 40.0 37.0 41.0 31.0 41.0 34-35 37.291540429808606 40.0 37.0 41.0 30.5 41.0 36-37 37.09178204957523 39.5 37.0 41.0 30.0 41.0 38-39 36.67302400779275 39.0 35.0 41.0 30.0 41.0 40-41 36.65196021483348 39.0 35.0 41.0 30.0 41.0 42-43 36.66928798805981 39.0 35.0 41.0 30.0 41.0 44-45 36.6059028721935 39.0 35.0 41.0 30.0 41.0 46-47 36.72737721473686 40.0 35.0 41.0 30.0 41.0 48-49 36.664215918939675 40.0 35.0 41.0 30.0 41.0 50-51 35.546077289736644 38.0 34.0 40.0 27.5 40.5 52-53 35.58791294632197 38.5 34.5 39.5 28.0 40.5 54-55 35.88924033464081 39.0 35.0 41.0 26.5 41.0 56-57 35.58612855832327 39.0 35.0 41.0 25.0 41.0 58-59 35.28687264675426 39.0 35.0 41.0 24.0 41.0 60-61 35.050494970635214 38.5 34.0 41.0 23.5 41.0 62-63 34.78853358366615 38.0 34.0 40.5 23.0 41.0 64-65 34.50378604991023 37.0 34.0 40.0 23.0 41.0 66-67 34.20960742164543 37.0 33.5 40.0 22.0 41.0 68-69 33.900147904148156 36.0 33.0 39.5 22.0 41.0 70-71 33.57005871918458 36.0 33.0 39.0 22.0 41.0 72-73 33.22744071082876 35.0 33.0 39.0 21.5 41.0 74-75 32.87291459439398 35.0 32.5 37.5 20.5 40.0 76-77 32.49430369071318 35.0 32.0 37.0 20.0 39.0 78-79 32.142337202615266 35.0 32.0 36.5 20.0 39.0 80-81 31.813173121111816 35.0 32.0 36.0 19.0 39.0 82-83 31.505131260667554 35.0 31.0 36.0 18.5 37.0 84-85 31.239993136119573 35.0 31.0 35.0 18.0 37.0 86-87 30.987645486293648 35.0 31.0 35.0 17.5 36.5 88-89 30.795044410229 35.0 31.0 35.0 17.0 36.0 90-91 30.624133543927893 35.0 30.5 35.0 17.0 36.0 92-93 30.463183019939756 34.0 30.0 35.0 15.5 36.0 94-95 30.301919449985125 34.0 30.0 35.0 15.0 35.0 96-97 30.184448301316294 34.0 30.0 35.0 15.0 35.0 98-99 30.07794004773399 34.0 30.0 35.0 14.0 35.0 100 29.95141335575725 34.0 29.0 35.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6611648552156915 1101 2 0.62177495005621 1101 3 0.6344974853522096 1101 4 0.5353911474402224 1101 5 0.5673929406418026 1101 6 0.5886076533998192 1101 7 0.5207189430212438 1101 8 0.5618672333740733 1101 9 0.7205468471390191 1101 10-11 0.6827092009472295 1101 12-13 0.6446969356473389 1101 14-15 0.8250666555006987 1101 16-17 0.8186101184579897 1101 18-19 0.765367719220464 1101 20-21 0.7342473985477298 1101 22-23 0.6927634980678832 1101 24-25 0.6597778489180612 1101 26-27 0.6708845590944676 1101 28-29 0.6565870014270985 1101 30-31 0.690337640136022 1101 32-33 0.7083379804363545 1101 34-35 0.6530064543497858 1101 36-37 0.6726493789241133 1101 38-39 0.681938264104005 1101 40-41 0.6492752648300382 1101 42-43 0.5853724491035948 1101 44-45 0.5678295340559742 1101 46-47 0.6214666741086461 1101 48-49 0.6533612418397965 1101 50-51 0.6736843188620796 1101 52-53 0.7001306703900525 1101 54-55 0.7347328148741852 1101 56-57 0.7225190219409114 1101 58-59 0.7500998119612916 1101 60-61 0.6922896273060601 1101 62-63 0.6556470324710517 1101 64-65 0.5941556645277117 1101 66-67 0.5592935220537711 1101 68-69 0.562033304174868 1101 70-71 0.5556206196998872 1101 72-73 0.5213203332857859 1101 74-75 0.5147639373496915 1101 76-77 0.47028527568600254 1101 78-79 0.44489118964202135 1101 80-81 0.4205947755057089 1101 82-83 0.37599200046703274 1101 84-85 0.3185748297793296 1101 86-87 0.30181725724884956 1101 88-89 0.3135431778440285 1101 90-91 0.2658708225469404 1101 92-93 0.2524492264771929 1101 94-95 0.21399511997974585 1101 96-97 0.15556668054919953 1101 98-99 0.14420809205606488 1101 100 0.1048004691467952 1102 1 0.640769959345473 1102 2 0.624591076655161 1102 3 0.6153487945802532 1102 4 0.5515441748327987 1102 5 0.5388864567149056 1102 6 0.5795551533172087 1102 7 0.5626786761867493 1102 8 0.6014386008522408 1102 9 0.7212063203296211 1102 10-11 0.6584376221514034 1102 12-13 0.6487877796058612 1102 14-15 0.795727751833617 1102 16-17 0.8139457630301337 1102 18-19 0.7977169540093811 1102 20-21 0.7506902643217046 1102 22-23 0.7206423398707784 1102 24-25 0.7445113723645207 1102 26-27 0.7507159139475306 1102 28-29 0.7771012770115888 1102 30-31 0.752815609835352 1102 32-33 0.7321526160796097 1102 34-35 0.7271486025519991 1102 36-37 0.7097734913539426 1102 38-39 0.7819899210901369 1102 40-41 0.7628721448255433 1102 42-43 0.7212163729941281 1102 44-45 0.6778191902807862 1102 46-47 0.7435820552303483 1102 48-49 0.765001686479934 1102 50-51 0.725388665525152 1102 52-53 0.7663913082122917 1102 54-55 0.8483167624115495 1102 56-57 0.8131463364337463 1102 58-59 0.8471551985146633 1102 60-61 0.8225810211359104 1102 62-63 0.762102316514131 1102 64-65 0.7256538294156769 1102 66-67 0.7213356026927258 1102 68-69 0.7062238894039794 1102 70-71 0.6782544392228758 1102 72-73 0.671646267209816 1102 74-75 0.6196737804305101 1102 76-77 0.6200170575851303 1102 78-79 0.5148007380354329 1102 80-81 0.5011158185213951 1102 82-83 0.5168624833727655 1102 84-85 0.49562956950991577 1102 86-87 0.4302279293469482 1102 88-89 0.46362497636353694 1102 90-91 0.4105967044941643 1102 92-93 0.36459400663193264 1102 94-95 0.3705558275796541 1102 96-97 0.34952431777552206 1102 98-99 0.31272161797996034 1102 100 0.29845933098132704 1103 1 0.5372435869881365 1103 2 0.5257681168274324 1103 3 0.519851489535565 1103 4 0.44849221639123016 1103 5 0.4606616266148862 1103 6 0.47973886998464366 1103 7 0.46902973188083763 1103 8 0.47888148555687593 1103 9 0.595178366627465 1103 10-11 0.5568775251140607 1103 12-13 0.5364072770814019 1103 14-15 0.6853583595165347 1103 16-17 0.6433446653886747 1103 18-19 0.6405486394784319 1103 20-21 0.5939480068557259 1103 22-23 0.6078428493351424 1103 24-25 0.604652203494787 1103 26-27 0.6142498322685128 1103 28-29 0.6031016459089926 1103 30-31 0.5925717690227756 1103 32-33 0.5754659520313368 1103 34-35 0.5520431859155011 1103 36-37 0.5957449842554325 1103 38-39 0.6314387434481787 1103 40-41 0.5593451891037589 1103 42-43 0.5638901543116575 1103 44-45 0.5835220853539695 1103 46-47 0.6156123208146056 1103 48-49 0.589463884970236 1103 50-51 0.5451058998556775 1103 52-53 0.6528441500728377 1103 54-55 0.74064064818063 1103 56-57 0.6899110296023565 1103 58-59 0.720505038256114 1103 60-61 0.6582804626748029 1103 62-63 0.6543973984017057 1103 64-65 0.6507909227837487 1103 66-67 0.569937198116186 1103 68-69 0.5771753097523558 1103 70-71 0.5642525059874259 1103 72-73 0.5347666102874058 1103 74-75 0.5357121074665585 1103 76-77 0.5111515373631974 1103 78-79 0.49874669701208063 1103 80-81 0.44824871039231695 1103 82-83 0.40880580026543356 1103 84-85 0.40362367557743184 1103 86-87 0.4002874503610947 1103 88-89 0.37837206827499514 1103 90-91 0.359746781017094 1103 92-93 0.3128203906674436 1103 94-95 0.39281175302344806 1103 96-97 0.3376788094502068 1103 98-99 0.31815566621658675 1103 100 0.3129980797023535 1104 1 0.5282841782899474 1104 2 0.5177127654647613 1104 3 0.48954698715772693 1104 4 0.4592934308985619 1104 5 0.480670124268606 1104 6 0.4538990528963822 1104 7 0.4477242119189455 1104 8 0.47742401352895314 1104 9 0.5847336812751323 1104 10-11 0.5532295648384036 1104 12-13 0.5573084463691558 1104 14-15 0.6935236750104892 1104 16-17 0.68964665835869 1104 18-19 0.7182046031610199 1104 20-21 0.6058536540684969 1104 22-23 0.5751978961822957 1104 24-25 0.6075426774348074 1104 26-27 0.6366128167053375 1104 28-29 0.6153169975717674 1104 30-31 0.6517672402165786 1104 32-33 0.6238628413295046 1104 34-35 0.618977115950031 1104 36-37 0.6118224637751482 1104 38-39 0.659980538299834 1104 40-41 0.5982595211983011 1104 42-43 0.5927436256144532 1104 44-45 0.5931898042238188 1104 46-47 0.6404005776347361 1104 48-49 0.6480137155200936 1104 50-51 0.6256481398487423 1104 52-53 0.6189553360662501 1104 54-55 0.741361124596601 1104 56-57 0.7384293965299733 1104 58-59 0.7218555896799259 1104 60-61 0.7202698662613543 1104 62-63 0.7054682308030067 1104 64-65 0.6683387070845583 1104 66-67 0.6524143211924738 1104 68-69 0.6576140580246559 1104 70-71 0.7105900763745936 1104 72-73 0.6837531110175163 1104 74-75 0.6149947636815511 1104 76-77 0.6107822037669095 1104 78-79 0.5885965641416746 1104 80-81 0.5858422448836116 1104 82-83 0.5820253323425568 1104 84-85 0.535120268175735 1104 86-87 0.5357586397626264 1104 88-89 0.5636346919154391 1104 90-91 0.5426802511093918 1104 92-93 0.4747005406722806 1104 94-95 0.4766252755153779 1104 96-97 0.5266218399613152 1104 98-99 0.43189133127237866 1104 100 0.41188000499580824 1105 1 0.40119403535459597 1105 2 0.39889589107065504 1105 3 0.3858301815035894 1105 4 0.36031159840004534 1105 5 0.36072517444834773 1105 6 0.3832050469297741 1105 7 0.3426298264325709 1105 8 0.3783532923953743 1105 9 0.45954347622650005 1105 10-11 0.4332230449723369 1105 12-13 0.44943561987989966 1105 14-15 0.5760280741679153 1105 16-17 0.5211899059517648 1105 18-19 0.5206756850256014 1105 20-21 0.5067847251129578 1105 22-23 0.5050867154838343 1105 24-25 0.5144720646685883 1105 26-27 0.5227589889159034 1105 28-29 0.5095091548636859 1105 30-31 0.5436997392983187 1105 32-33 0.496776475548792 1105 34-35 0.5354194005329731 1105 36-37 0.52264012190156 1105 38-39 0.5396629742961139 1105 40-41 0.4726965970789294 1105 42-43 0.452566998102931 1105 44-45 0.4958290088784878 1105 46-47 0.541232643575043 1105 48-49 0.5369941045150028 1105 50-51 0.48928270563005327 1105 52-53 0.5460536074626532 1105 54-55 0.6153546091321616 1105 56-57 0.5941828175938184 1105 58-59 0.6304547460309138 1105 60-61 0.589127650635433 1105 62-63 0.5151773443635648 1105 64-65 0.44818617321189436 1105 66-67 0.4684669689320984 1105 68-69 0.516749786475394 1105 70-71 0.5583223845869512 1105 72-73 0.5071265065020754 1105 74-75 0.4816856726481191 1105 76-77 0.4130750463723203 1105 78-79 0.4346528087874617 1105 80-81 0.44090786791335645 1105 82-83 0.46325102460178513 1105 84-85 0.4169911281558214 1105 86-87 0.4308956028760953 1105 88-89 0.3991187055752512 1105 90-91 0.29426262032400174 1105 92-93 0.32105232978341647 1105 94-95 0.372148006768505 1105 96-97 0.35273871078864616 1105 98-99 0.34155373543518763 1105 100 0.33126959546439494 1106 1 0.35975883902072425 1106 2 0.3375973939510253 1106 3 0.28426725645668327 1106 4 0.32077852356390935 1106 5 0.3146853332581827 1106 6 0.33014644862491593 1106 7 0.31683255434332835 1106 8 0.3484823820092302 1106 9 0.42697430096192335 1106 10-11 0.38716379705741133 1106 12-13 0.37615954959157705 1106 14-15 0.42309880954153556 1106 16-17 0.44605232598618727 1106 18-19 0.412088873558595 1106 20-21 0.4223860552334884 1106 22-23 0.33784032442201806 1106 24-25 0.37137868356732184 1106 26-27 0.4059192390545121 1106 28-29 0.4197528604305063 1106 30-31 0.42662883648823424 1106 32-33 0.37166973786749224 1106 34-35 0.3520301956399834 1106 36-37 0.36806099039050366 1106 38-39 0.4115561551542797 1106 40-41 0.38389335361404164 1106 42-43 0.37277540538482157 1106 44-45 0.3151636608948536 1106 46-47 0.35373414604057984 1106 48-49 0.33832491681474863 1106 50-51 0.35593411283557685 1106 52-53 0.4157697212617606 1106 54-55 0.45360835823930046 1106 56-57 0.48964398455072455 1106 58-59 0.5191289093909646 1106 60-61 0.5349727717370669 1106 62-63 0.496984770230938 1106 64-65 0.4461669779875663 1106 66-67 0.43758237789769794 1106 68-69 0.45326423894675116 1106 70-71 0.48174106419040896 1106 72-73 0.40811969217202915 1106 74-75 0.39866276044015336 1106 76-77 0.4265694525739434 1106 78-79 0.3644405403464219 1106 80-81 0.3329215255008684 1106 82-83 0.3896885367692171 1106 84-85 0.40550322793090743 1106 86-87 0.3920771836404917 1106 88-89 0.3447685377158969 1106 90-91 0.3191565051771157 1106 92-93 0.3375063791228534 1106 94-95 0.3637824310740676 1106 96-97 0.30767864311351545 1106 98-99 0.29131631905074684 1106 100 0.38155831435625487 1107 1 0.4330072454941778 1107 2 0.39591019794880467 1107 3 0.33873154992375376 1107 4 0.38782472616873775 1107 5 0.4056577740842684 1107 6 0.4281735557199653 1107 7 0.4042458344462716 1107 8 0.4065365414954485 1107 9 0.5436467703097918 1107 10-11 0.48798640782531066 1107 12-13 0.4552256766680429 1107 14-15 0.5414358174931451 1107 16-17 0.5976294912898013 1107 18-19 0.5990508991663077 1107 20-21 0.5426165928720437 1107 22-23 0.51222495203519 1107 24-25 0.580895722118612 1107 26-27 0.5590297783883145 1107 28-29 0.5469435804801037 1107 30-31 0.5471780748169479 1107 32-33 0.5148337540406089 1107 34-35 0.5019311019311843 1107 36-37 0.5085168755148075 1107 38-39 0.5848809210226804 1107 40-41 0.5895605790539946 1107 42-43 0.5614619976048587 1107 44-45 0.5500389498533806 1107 46-47 0.5546680300968916 1107 48-49 0.5528468346685713 1107 50-51 0.5077926631620002 1107 52-53 0.5470838487513277 1107 54-55 0.6320995363666171 1107 56-57 0.6436995421882301 1107 58-59 0.6451024450270566 1107 60-61 0.6216036442767603 1107 62-63 0.6335425261929828 1107 64-65 0.6041725088485919 1107 66-67 0.5707812680079556 1107 68-69 0.6091511280461006 1107 70-71 0.6097383932105132 1107 72-73 0.5756310694194369 1107 74-75 0.5850232495800967 1107 76-77 0.6029689076246925 1107 78-79 0.6012133489901785 1107 80-81 0.5893796408280032 1107 82-83 0.5399997294792662 1107 84-85 0.5126798050422927 1107 86-87 0.5564736134003958 1107 88-89 0.5773335247086742 1107 90-91 0.5937927743534352 1107 92-93 0.5869291173043258 1107 94-95 0.5793386824585802 1107 96-97 0.6013527834402126 1107 98-99 0.59693840612805 1107 100 0.5745922440779339 1108 1 0.19406726353100012 1108 2 0.1647484947353881 1108 3 0.17515519445966632 1108 4 0.1844131631589434 1108 5 0.18335959059962192 1108 6 0.21168909349003684 1108 7 0.20583735708074613 1108 8 0.2111116916601432 1108 9 0.25831188421497586 1108 10-11 0.22797116136314344 1108 12-13 0.20678303601724224 1108 14-15 0.28351055257131463 1108 16-17 0.2709025773665772 1108 18-19 0.27956168950568383 1108 20-21 0.23256579976919767 1108 22-23 0.1900209039411962 1108 24-25 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-1.8134358212985902 2316 72-73 -1.9513653791684504 2316 74-75 -1.7346199180603747 2316 76-77 -1.6489425666996276 2316 78-79 -1.5391960518906487 2316 80-81 -1.5128377884831714 2316 82-83 -1.7682954736133887 2316 84-85 -1.4612753152078994 2316 86-87 -1.3865835069307835 2316 88-89 -1.495964072192951 2316 90-91 -1.4265170869485573 2316 92-93 -1.3471339506298285 2316 94-95 -1.3374836016400984 2316 96-97 -1.2580835876883079 2316 98-99 -1.2732159171385824 2316 100 -1.4212072762075145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 32.0 4 84.0 5 160.0 6 1966.0 7 23018.0 8 35457.0 9 44981.0 10 48464.0 11 47277.0 12 48930.0 13 53569.0 14 64074.0 15 82140.0 16 100856.0 17 120076.0 18 129760.0 19 143130.0 20 162870.0 21 196846.0 22 255884.0 23 360610.0 24 483096.0 25 541977.0 26 529955.0 27 480909.0 28 463640.0 29 493421.0 30 565299.0 31 677468.0 32 832873.0 33 1064125.0 34 1441026.0 35 2105024.0 36 3422855.0 37 6099372.0 38 7418114.0 39 2235488.0 40 6596.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.259063433675664 34.37218399154294 27.21297941718731 20.15577315759409 2 21.751287216164393 32.553268391515424 26.41067084053544 19.284773551784745 3 25.72572828190432 26.296375171967462 26.279987803691412 21.697908742436802 4 20.641681115885575 26.986130309235158 25.94659223028583 26.425596344593437 5 21.258001588175496 31.893732132172875 25.942765190725144 20.90550108892649 6 26.635205482059927 26.481703801728194 25.94504243501161 20.938048281200263 7 21.078763712695395 26.670172244484746 31.305185683273034 20.945878359546818 8 26.185333478095973 26.674628612024488 26.08348308275037 21.05655482712917 9 26.179463054046042 26.424838332680018 26.28422104735772 21.11147756591622 10-11 20.710658549519206 29.575367881814163 28.84107429608269 20.87289927258394 12-13 20.72409181722451 26.820957607730435 28.79760981740475 23.65734075764031 14-15 20.76143525792928 32.26814050371033 26.029835463741733 20.940588774618664 16-17 23.59027143060512 26.74491613805236 25.98461013269627 23.68020229864624 18-19 20.865588990658065 29.490370847714576 25.915857623471716 23.728182538155647 20-21 23.604675743344387 29.509506556254937 25.918791932534624 20.967025767866048 22-23 23.630166308102844 26.850040993236828 28.588274528134804 20.931518170525525 24-25 20.96935692323186 29.47928619094074 25.808758139227823 23.742598746599576 26-27 21.03110978458372 26.796723909938187 31.17318642548011 20.998979879997982 28-29 21.002951689394937 26.867187172331413 31.17586295773542 20.953998180538232 30-31 21.009657859587914 26.839728388613043 31.156056174219003 20.994557577580032 32-33 21.01198106442825 26.863624947102792 31.12731505670959 20.997078931759365 34-35 21.0387890869134 26.909523827630245 31.095656014980356 20.956031070476 36-37 21.090121068269433 26.871302279203015 31.020081114287862 21.018495538239698 38-39 21.11288948904969 26.888567411835208 30.99129891248716 21.007244186627943 40-41 21.093198900651238 26.985907931873587 30.982095849716075 20.9387973177591 42-43 21.15393380776888 27.027924894237724 30.903096315175638 20.915044982817754 44-45 21.199723131687524 27.078496360576437 30.825864754250926 20.895915753485117 46-47 21.24007878612334 27.151441618375234 30.75628103092558 20.852198564575843 48-49 21.33899627274856 27.1497112391604 30.618809206614912 20.892483281476135 50-51 21.393528212124913 27.1936204529223 30.555307635732852 20.857543699219935 52-53 21.529166174278828 27.336655773934655 30.34196475608486 20.79221329570166 54-55 21.910638969515777 27.52352456536793 29.667765345752773 20.898071119363525 56-57 22.343116448889212 27.93331754968571 28.739602944584565 20.983963056840516 58-59 22.583778714784355 28.338190747489296 28.143200322977215 20.934830214749137 60-61 22.790453359332773 28.533252601934688 27.767258359946645 20.9090356787859 62-63 22.945286562457408 28.65507525935267 27.57755122026587 20.822086957924054 64-65 23.12496042450107 28.708456377157276 27.4887407197386 20.67784247860305 66-67 23.311411647100403 28.551771811215037 27.523567847674713 20.61324869400985 68-69 23.4828316110169 28.38399724830692 27.622344231486768 20.510826909189415 70-71 23.6547544861181 28.28757972116885 27.6815439645733 20.376121828139745 72-73 23.850185253373493 28.197567454406773 27.669389064067868 20.282858228151866 74-75 24.069263720316293 28.198810703158035 27.575515942085605 20.156409634440067 76-77 24.38701322694983 28.266267026444282 27.32580975315605 20.02090999344984 78-79 24.677362597946644 28.325845388403025 27.01630358315984 19.980488430490492 80-81 24.95418022116997 28.402809222405395 26.71511511886297 19.927895437561663 82-83 25.139391381097493 28.49306407728065 26.505104346541035 19.86244019508082 84-85 25.32445727955588 28.498966401549747 26.332966590067702 19.84360972882667 86-87 25.513156984829166 28.4492886384574 26.20953483932983 19.828019537383607 88-89 25.7370302415323 28.41876523083252 26.089551444099413 19.75465308353576 90-91 25.93104153752921 28.35371504922659 25.972739334113687 19.74250407913051 92-93 26.058921989175715 28.33222354505489 25.938916312711058 19.669938153058343 94-95 26.21575524607531 28.337949370913023 25.866820022640972 19.579475360370694 96-97 26.32661742398565 28.331227519801967 25.76964964074671 19.57250541546568 98-99 26.421816902962657 28.345153414573065 25.684369946250985 19.548659736213295 100 26.47848915392375 28.441350526737725 25.55840621452612 19.521754104812402 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 63765.0 1 39036.5 2 11996.5 3 9374.0 4 9014.0 5 9188.5 6 9637.5 7 10034.0 8 10563.5 9 10915.5 10 11695.0 11 13969.0 12 84064.0 13 104905.0 14 57974.5 15 62805.5 16 70912.5 17 79516.0 18 88421.5 19 97489.0 20 108388.5 21 122965.0 22 142930.0 23 167411.5 24 192666.5 25 221474.5 26 255554.0 27 295670.5 28 342605.5 29 390490.0 30 436842.5 31 482537.0 32 530243.0 33 576664.5 34 622832.5 35 675140.5 36 731156.0 37 782052.5 38 831247.5 39 872365.0 40 900957.0 41 938376.0 42 982903.0 43 1006598.0 44 1020852.5 45 1047527.0 46 1075936.0 47 1101574.0 48 1114919.5 49 1114198.0 50 1107273.5 51 1092089.5 52 1064804.0 53 1018633.0 54 956944.5 55 888163.5 56 813429.0 57 731132.5 58 641455.5 59 547904.5 60 452859.0 61 364968.0 62 287004.0 63 218257.0 64 163630.5 65 121915.0 66 89797.0 67 66812.0 68 50511.0 69 38755.5 70 30228.0 71 24105.0 72 20056.0 73 16226.0 74 12900.5 75 11037.5 76 8988.5 77 6513.0 78 4756.5 79 3726.5 80 3021.5 81 2449.0 82 2027.0 83 1648.0 84 1368.5 85 1198.0 86 998.0 87 804.0 88 704.0 89 645.0 90 552.5 91 478.0 92 420.0 93 364.5 94 326.5 95 278.0 96 237.5 97 193.5 98 148.0 99 109.5 100 74.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04628441142192846 2 0.014749156163090842 3 0.005253168615796892 4 1.8842534305270236E-4 5 6.140067213269094E-4 6 0.0 7 3.248712811253489E-6 8 0.0 9 0.0 10-11 3.248712811253489E-6 12-13 2.598970249002791E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.03795633613028014 22-23 0.07569338414580067 24-25 0.03148490021026319 26-27 0.035808936962041585 28-29 0.040389622025909004 30-31 0.03402539362866342 32-33 0.034626405498745315 34-35 0.03570010508286459 36-37 0.033367529284384585 38-39 0.043440163355676034 40-41 0.0641799459427183 42-43 0.07258923905464797 44-45 0.07510049405774691 46-47 0.04819952762416239 48-49 0.030593128543574107 50-51 0.028278420665555998 52-53 0.028466846008608698 54-55 0.029287145993450205 56-57 0.029457703416041016 58-59 0.037196137332446824 60-61 0.05743399379015044 62-63 0.08071751850840418 64-65 0.1079579754307647 66-67 0.0895117840884674 68-69 0.053158687730540846 70-71 0.0465962878518088 72-73 0.05063281351979126 74-75 0.04820764940619053 76-77 0.05116722677724246 78-79 0.043932343346580936 80-81 0.042395702186858036 82-83 0.04171672120930606 84-85 0.04912703513177526 86-87 0.052952394467026244 88-89 0.05901611692923089 90-91 0.05589897698683316 92-93 0.05131017014093761 94-95 0.0532772657481516 96-97 0.05518101145554614 98-99 0.049630585617519554 100 0.05141412895089772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0781422E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.686495875736725 #Duplication Level Percentage of deduplicated Percentage of total 1 59.53927037042671 24.2244427837074 2 20.11437043534066 16.36766499521057 3 8.837587736627084 10.78711430993218 4 4.12950415875244 6.720602156956752 5 2.0930211140276467 4.257884746185786 6 1.2067505640923908 2.9459071109392814 7 0.8051064726677059 2.2929872825806488 8 0.5745994015193223 1.8702748944093357 9 0.4033400415563004 1.4769443643569902 >10 2.0683041586549757 14.636335227039782 >50 0.1501682087153117 4.340676796528696 >100 0.07706020823168892 4.473775521000242 >500 3.9098153891247215E-4 0.10571389855944383 >1k 4.7854309287116123E-4 0.3886840876064135 >5k 1.586825200889526E-5 0.037575291768985004 >10k+ 3.1736503973600776E-5 5.073416533217442 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1422850 4.622431023492027 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112897 0.36676993025208515 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.005412355543548313 1.2994851245013957E-5 0.0 3.2487128112534893E-6 4.548197935754885E-5 2 0.005428599107604581 2.5989702490027915E-5 0.0 3.2487128112534893E-6 4.548197935754885E-5 3 0.005487075938207143 4.548197935754885E-5 0.0 3.2487128112534893E-6 4.873069216880234E-5 4 0.0055065682150746646 5.197940498005583E-5 0.0 3.2487128112534893E-6 5.197940498005583E-5 5 0.005542304055998453 5.522811779130931E-5 0.0 3.2487128112534893E-6 6.17255434138163E-5 6 0.005574791184110988 5.522811779130931E-5 0.0 6.497425622506979E-6 6.17255434138163E-5 7 0.005581288609733495 5.8476830602562804E-5 0.0 9.746138433760468E-6 6.17255434138163E-5 8 0.005620273163468537 6.17255434138163E-5 0.0 9.746138433760468E-6 6.17255434138163E-5 9 0.005643014153147311 7.147168184757677E-5 0.0 9.746138433760468E-6 6.17255434138163E-5 10-11 0.005716110191400514 8.121782028133723E-5 0.0 1.2994851245013957E-5 6.659861263069654E-5 12-13 0.005792454942464971 9.42126715263512E-5 0.0 1.6243564056267446E-5 7.309603825320351E-5 14-15 0.005867175337123802 1.0233445355448492E-4 0.0 1.9492276867520937E-5 8.121782028133723E-5 16-17 0.005961388008650153 1.0395880996011166E-4 0.0 1.9492276867520937E-5 8.121782028133723E-5 18-19 0.0060685955314215174 1.1208059198824537E-4 0.0 2.2740989678774424E-5 8.121782028133723E-5 20-21 0.006166056915759123 1.2182673042200585E-4 0.0 2.436534608440117E-5 9.09639587150977E-5 22-23 0.006406461663791881 1.2507544323325933E-4 0.0 2.5989702490027915E-5 9.583702793197792E-5 24-25 0.007374578081545421 1.3319722526139305E-4 0.0 2.761405889565466E-5 9.746138433760468E-5 26-27 0.010683392079807098 1.4294336369515353E-4 0.0 2.9238415301281402E-5 9.746138433760468E-5 28-29 0.020041309332622777 1.5431385853454074E-4 0.0 2.9238415301281402E-5 9.746138433760468E-5 30-31 0.042397326543263655 1.7380613540206167E-4 0.0 3.086277170690815E-5 1.0071009714885816E-4 32-33 0.08541028416425986 1.997958378920896E-4 0.0 3.248712811253489E-5 1.0720752277136514E-4 34-35 0.15834713548971197 2.3065860959899773E-4 0.0 3.573584092378838E-5 1.1045623558261863E-4 36-37 0.27142345795460654 2.469021736552652E-4 0.0 4.0608910140668616E-5 1.1045623558261863E-4 38-39 0.42653974855352683 2.469021736552652E-4 0.0 4.548197935754885E-5 1.1045623558261863E-4 40-41 0.6322726091081822 2.647700941171594E-4 8.121782028133723E-6 4.548197935754885E-5 1.1045623558261863E-4 42-43 0.8836206462456477 2.777649453621733E-4 1.6243564056267446E-5 4.548197935754885E-5 1.1045623558261863E-4 44-45 1.1860628141221028 2.9075979660718725E-4 1.6243564056267446E-5 4.71063357631756E-5 1.2020237401637909E-4 46-47 1.5393180990793733 2.9238415301281403E-4 1.6243564056267446E-5 5.360376138568257E-5 1.2994851245013957E-4 48-49 1.9444423327811173 2.9238415301281403E-4 1.6243564056267446E-5 6.17255434138163E-5 1.3969465088390005E-4 50-51 2.404382097747141 2.9238415301281403E-4 1.6243564056267446E-5 6.17255434138163E-5 1.4131900728952678E-4 52-53 2.9058712752126916 2.98881578635321E-4 1.6243564056267446E-5 6.497425622506979E-5 1.4294336369515353E-4 54-55 3.4527368488694252 3.021302914465745E-4 1.6243564056267446E-5 7.147168184757677E-5 1.4294336369515353E-4 56-57 4.0399595574239555 3.0700336066345475E-4 1.9492276867520937E-5 7.147168184757677E-5 1.4294336369515353E-4 58-59 4.673513783736176 3.1674949909721523E-4 2.2740989678774424E-5 7.147168184757677E-5 1.5106514572328726E-4 60-61 5.3489699078879465 3.248712811253489E-4 2.2740989678774424E-5 7.472039465883026E-5 1.559382149401675E-4 62-63 6.051869533512779 3.248712811253489E-4 2.2740989678774424E-5 9.42126715263512E-5 1.5756257134579422E-4 64-65 6.783406562568812 3.281199939366024E-4 2.2740989678774424E-5 1.0071009714885816E-4 1.6893306618518143E-4 66-67 7.545697531452575 3.346174195591094E-4 2.2740989678774424E-5 1.0071009714885816E-4 1.7055742259080816E-4 68-69 8.337585898403265 3.346174195591094E-4 2.2740989678774424E-5 1.0071009714885816E-4 1.7543049180768843E-4 70-71 9.158449534917523 3.3624177596473613E-4 2.2740989678774424E-5 1.0233445355448492E-4 1.868009866470756E-4 72-73 9.99728667506004 3.573584092378838E-4 2.2740989678774424E-5 1.0395880996011166E-4 1.9817148148646284E-4 74-75 10.854911121390039 3.8497246813353847E-4 2.2740989678774424E-5 1.1857801761075236E-4 2.2091247116523728E-4 76-77 11.731188052325848 3.865968245391652E-4 2.2740989678774424E-5 1.202023740163791E-4 2.2903425319337098E-4 78-79 12.62449798453106 3.865968245391652E-4 2.2740989678774424E-5 1.202023740163791E-4 2.3390732241025122E-4 80-81 13.538297548436846 3.8822118094479193E-4 2.2740989678774424E-5 1.202023740163791E-4 2.3390732241025122E-4 82-83 14.46284710303507 4.2558137827420706E-4 2.2740989678774424E-5 1.202023740163791E-4 2.3553167881587794E-4 84-85 15.392901601491964 4.4669801154735475E-4 2.2740989678774424E-5 1.2669979963888609E-4 2.371560352215047E-4 86-87 16.33280619719258 4.64565932009249E-4 2.2740989678774424E-5 1.2669979963888609E-4 2.371560352215047E-4 88 17.046389214897218 4.938043473105304E-4 2.2740989678774424E-5 1.2669979963888609E-4 2.371560352215047E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 182290 0.0 85.171616 8 AGTGGTA 183765 0.0 84.5344 5 GTGGTAT 184910 0.0 84.02324 6 TGGTATC 184390 0.0 83.99781 7 GTATCAA 184865 0.0 83.83534 9 AAGCAGT 194960 0.0 79.96623 1 GCAGTGG 195855 0.0 79.8301 3 CAGTGGT 196065 0.0 79.17848 4 AGCAGTG 202650 0.0 77.17245 2 CAACGCA 178720 0.0 44.193398 12-13 ATCAACG 180020 0.0 43.976025 10-11 ACGCAGA 181160 0.0 43.60885 14-15 TCAACGC 178875 0.0 43.464485 12-13 GAGTACT 175200 0.0 43.30541 20-21 AACGCAG 179845 0.0 43.295433 14-15 AGAGTAC 181855 0.0 42.915108 18-19 CGCAGAG 182410 0.0 42.654537 16-17 AGTACTT 184250 0.0 41.889133 20-21 TATCAAC 185200 0.0 41.81268 10-11 GTACTTT 183545 0.0 41.34451 22-23 >>END_MODULE