FastQCFastQC Report
Thu 26 May 2016
SRR1660762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30781422
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15729315.110001090917763No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA874840.28421039157970024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1998350.085.398148
AGTGGTA2018450.084.923045
GTGGTAT2026150.084.544186
GTATCAA2019800.084.346989
TGGTATC2025450.084.290347
GCAGTGG2118350.080.995773
AAGCAGT2079150.080.842481
CAGTGGT2140400.079.961564
AGCAGTG2191100.078.203562
CAACGCA1956850.044.2927112-13
ATCAACG1968950.044.0956210-11
ACGCAGA1986500.043.74817714-15
TCAACGC1951250.043.65878312-13
GAGTACT1934100.043.51669320-21
AACGCAG1965700.043.38610514-15
AGAGTAC1997500.043.2645118-19
CGCAGAG2006600.042.64818616-17
AGTACTT2028000.042.26781520-21
TATCAAC2025800.042.0659810-11
GTACTTT2026100.041.52855722-23
Produced by FastQC (version 0.11.5)