##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660762_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30781422 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.948750060994584 33.0 31.0 34.0 26.0 34.0 2 31.742764710480238 34.0 31.0 34.0 28.0 34.0 3 32.257622016292814 34.0 31.0 34.0 30.0 34.0 4 35.796840152478985 37.0 35.0 37.0 35.0 37.0 5 35.85682747210314 37.0 35.0 37.0 35.0 37.0 6 35.896795118822 37.0 35.0 37.0 35.0 37.0 7 35.89271954362602 37.0 35.0 37.0 35.0 37.0 8 35.89851924319806 37.0 35.0 37.0 35.0 37.0 9 37.60563407369549 39.0 37.0 39.0 35.0 39.0 10-11 37.545920506856376 39.0 37.0 39.0 35.0 39.0 12-13 37.60676641254585 39.0 37.0 39.0 35.0 39.0 14-15 38.92594859652682 40.0 38.0 41.0 35.0 41.0 16-17 38.94013479949042 40.0 38.0 41.0 35.0 41.0 18-19 38.922647319542286 40.0 38.0 41.0 35.0 41.0 20-21 38.85802381059588 40.0 38.0 41.0 35.0 41.0 22-23 38.755402950519965 40.0 38.0 41.0 34.5 41.0 24-25 38.69635467133389 40.0 38.0 41.0 34.0 41.0 26-27 38.6520722791819 40.0 38.0 41.0 34.0 41.0 28-29 38.54547905551602 40.0 38.0 41.0 34.0 41.0 30-31 38.44541972427395 40.0 38.0 41.0 34.0 41.0 32-33 38.33751311099273 40.0 38.0 41.0 34.0 41.0 34-35 38.22646783504673 40.0 38.0 41.0 33.5 41.0 36-37 38.04066842655937 40.0 38.0 41.0 33.0 41.0 38-39 37.6339297775132 40.0 36.0 41.0 33.0 41.0 40-41 37.48333244643474 40.0 36.0 41.0 33.0 41.0 42-43 37.32361820386336 40.0 35.5 41.0 32.0 41.0 44-45 37.20441212884837 39.0 35.0 41.0 32.0 41.0 46-47 37.06844729265594 39.0 35.0 41.0 31.0 41.0 48-49 37.186429398875724 39.0 35.0 41.0 32.0 41.0 50-51 37.09060702263852 39.0 35.0 41.0 31.0 41.0 52-53 36.8338300940093 39.0 35.0 41.0 31.0 41.0 54-55 36.357501108948114 39.0 35.0 41.0 29.5 41.0 56-57 36.081179274303835 39.0 35.0 41.0 28.0 41.0 58-59 35.883588548963075 39.0 35.0 41.0 28.0 41.0 60-61 35.68030102702858 39.0 35.0 41.0 27.0 41.0 62-63 35.384846694217046 38.0 35.0 40.5 26.5 41.0 64-65 35.13362577921189 37.5 34.5 40.0 26.0 41.0 66-67 34.858287947191 37.0 34.0 40.0 26.0 41.0 68-69 34.55456690727283 36.5 34.0 39.5 26.0 41.0 70-71 34.2163061050266 36.0 34.0 39.0 26.0 41.0 72-73 33.89715647314799 35.5 34.0 39.0 26.0 40.5 74-75 33.54379440300062 35.0 33.0 37.5 25.0 40.0 76-77 31.458617197737 33.5 30.0 35.5 21.5 38.5 78-79 32.72048593141669 35.0 33.0 37.0 24.0 39.0 80-81 32.70628881277804 35.0 33.0 36.0 24.5 39.0 82-83 32.49261088392862 35.0 33.0 36.0 24.0 37.0 84-85 32.26032164790827 35.0 33.0 35.5 24.0 37.0 86-87 32.05434942219369 35.0 33.0 35.0 23.5 37.0 88-89 31.836726825031022 35.0 33.0 35.0 23.0 36.0 90-91 31.676983538967107 35.0 33.0 35.0 22.0 36.0 92-93 31.55311903719068 35.0 33.0 35.0 20.0 36.0 94-95 31.454029722863353 35.0 32.5 35.0 20.0 35.0 96-97 31.347073780412096 35.0 32.0 35.0 20.0 35.0 98-99 31.226514600267656 35.0 32.0 35.0 19.5 35.0 100 31.037241586824678 35.0 32.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7390874661863656 1101 2 1.197049652285223 1101 3 0.7361153636143882 1101 4 0.5843990905597067 1101 5 0.44225110196197903 1101 6 0.39950299265390754 1101 7 0.3883705446661452 1101 8 0.40953286531711086 1101 9 0.49449030661075 1101 10-11 0.48376273406552883 1101 12-13 0.44800120507903785 1101 14-15 0.5889117960995733 1101 16-17 0.5914582939933126 1101 18-19 0.5263054169248349 1101 20-21 0.5766943881989803 1101 22-23 0.54196279930499 1101 24-25 0.5897888337420056 1101 26-27 0.5480195160082317 1101 28-29 0.5654948955804997 1101 30-31 0.5439178533920739 1101 32-33 0.5415409529018902 1101 34-35 0.5179245490172164 1101 36-37 0.5268947737890883 1101 38-39 0.5977955994997686 1101 40-41 0.5943714000891589 1101 42-43 0.5722565634356798 1101 44-45 0.5333813282076463 1101 46-47 0.5145326574056384 1101 48-49 0.43168105717928995 1101 50-51 0.48097435975903835 1101 52-53 0.4918182119898944 1101 54-55 0.5492148744761423 1101 56-57 0.6284460570384098 1101 58-59 0.6547493384337031 1101 60-61 0.6255352771689147 1101 62-63 0.6163838010104996 1101 64-65 0.5717073368428842 1101 66-67 0.5567589461217821 1101 68-69 0.5692652750691352 1101 70-71 0.5598526212608874 1101 72-73 0.5150363006748719 1101 74-75 0.48556075170129986 1101 76-77 0.3737182526884233 1101 78-79 0.5176098862607077 1101 80-81 0.5103992503573593 1101 82-83 0.43759442506598845 1101 84-85 0.469969194320079 1101 86-87 0.3937049228890501 1101 88-89 0.42972599406491696 1101 90-91 0.4694811728825954 1101 92-93 0.3898969037098112 1101 94-95 0.3527885887882931 1101 96-97 0.3464380799942326 1101 98-99 0.3457639717602419 1101 100 0.28171464911381605 1102 1 1.6783386962120126 1102 2 1.1657852196834142 1102 3 0.7280365534795408 1102 4 0.5868897388208012 1102 5 0.4497305949359429 1102 6 0.4169412438715625 1102 7 0.3714781484537397 1102 8 0.3937204940777619 1102 9 0.46817352644775667 1102 10-11 0.48152458892172234 1102 12-13 0.41747801353854186 1102 14-15 0.5895865788805423 1102 16-17 0.5587887563668374 1102 18-19 0.5487342370350419 1102 20-21 0.5502656540652922 1102 22-23 0.5400608662394148 1102 24-25 0.5801488666248744 1102 26-27 0.5902361127111035 1102 28-29 0.5842863117425097 1102 30-31 0.5651011924262264 1102 32-33 0.5539268779088573 1102 34-35 0.5565319616111282 1102 36-37 0.5834204480417284 1102 38-39 0.6473756079831787 1102 40-41 0.5791411945314948 1102 42-43 0.5380236878765743 1102 44-45 0.5628792117544918 1102 46-47 0.607157689105307 1102 48-49 0.5856884848950514 1102 50-51 0.5265469800591802 1102 52-53 0.45564729257220904 1102 54-55 0.5243931209719861 1102 56-57 0.605564108587977 1102 58-59 0.569260510275619 1102 60-61 0.54467520297829 1102 62-63 0.6024332499332061 1102 64-65 0.5542156900974149 1102 66-67 0.5187979232372939 1102 68-69 0.5324487691773001 1102 70-71 0.5328186400911292 1102 72-73 0.4688386485803022 1102 74-75 0.48165714817877614 1102 76-77 0.42286838963922335 1102 78-79 0.526869309375023 1102 80-81 0.506095956960344 1102 82-83 0.4772757151069129 1102 84-85 0.46257454001861475 1102 86-87 0.4317131249265813 1102 88-89 0.478378707026053 1102 90-91 0.47383261227621887 1102 92-93 0.427751963204134 1102 94-95 0.39956648842389697 1102 96-97 0.3867158377466495 1102 98-99 0.41055590683149745 1102 100 0.32773400000370856 1103 1 1.6258712073892632 1103 2 1.0741676107005702 1103 3 0.6304706037514549 1103 4 0.5323990893153834 1103 5 0.37877732943051257 1103 6 0.33751574671082807 1103 7 0.31129173867783066 1103 8 0.35223715900773556 1103 9 0.41225906565043147 1103 10-11 0.38737661674052504 1103 12-13 0.37549784501514694 1103 14-15 0.5173655300877513 1103 16-17 0.4634220215198184 1103 18-19 0.3924012936973966 1103 20-21 0.4060187699915474 1103 22-23 0.4494035618309411 1103 24-25 0.4957632409795707 1103 26-27 0.4423492878267794 1103 28-29 0.45810149456411153 1103 30-31 0.450846766660419 1103 32-33 0.42556264171270897 1103 34-35 0.45759509704755175 1103 36-37 0.4417959044343789 1103 38-39 0.5326347827376523 1103 40-41 0.5107195025067313 1103 42-43 0.47328639255133 1103 44-45 0.49346062610145935 1103 46-47 0.46541025787088586 1103 48-49 0.44175335949459793 1103 50-51 0.39812856383729667 1103 52-53 0.3901146009665766 1103 54-55 0.4202541426541231 1103 56-57 0.4626177796502091 1103 58-59 0.43526319093787436 1103 60-61 0.44819031803496756 1103 62-63 0.4667289572142792 1103 64-65 0.4470482189950431 1103 66-67 0.4237976777886914 1103 68-69 0.4506162612942006 1103 70-71 0.4330227137908409 1103 72-73 0.39967258477242495 1103 74-75 0.3510474425404553 1103 76-77 0.2110741776012297 1103 78-79 0.35101159549077465 1103 80-81 0.38421458541549924 1103 82-83 0.37438599312501 1103 84-85 0.3612769695477027 1103 86-87 0.36246928896350283 1103 88-89 0.4121451593588432 1103 90-91 0.39435137302987755 1103 92-93 0.3798055865670129 1103 94-95 0.36515931179652483 1103 96-97 0.3348581595091922 1103 98-99 0.34316215489844737 1103 100 0.26804666207527106 1104 1 1.5839546421715376 1104 2 1.0759335092116444 1104 3 0.6250934147485978 1104 4 0.5202362542253027 1104 5 0.3890369887777183 1104 6 0.34021374704819607 1104 7 0.3158775241051188 1104 8 0.31384905630176974 1104 9 0.4100867225657012 1104 10-11 0.38391483460625153 1104 12-13 0.36540556196796814 1104 14-15 0.5202982890013388 1104 16-17 0.5032314887752349 1104 18-19 0.4434256041735267 1104 20-21 0.4159605557314947 1104 22-23 0.4224905180213483 1104 24-25 0.4699226315518672 1104 26-27 0.4433514222358852 1104 28-29 0.4588194327413291 1104 30-31 0.42027399938193355 1104 32-33 0.4638072287426027 1104 34-35 0.41066529991845613 1104 36-37 0.48427844774158046 1104 38-39 0.5150079139166053 1104 40-41 0.5130247010579936 1104 42-43 0.5002326084478668 1104 44-45 0.4995365562535028 1104 46-47 0.5357445037669208 1104 48-49 0.5139265859100064 1104 50-51 0.49406465759945206 1104 52-53 0.46031126685096524 1104 54-55 0.47727699354270925 1104 56-57 0.5147825280729208 1104 58-59 0.49265903709265757 1104 60-61 0.45365831149702984 1104 62-63 0.5024323209464683 1104 64-65 0.48782726789500686 1104 66-67 0.488200291717682 1104 68-69 0.4828923617594967 1104 70-71 0.508548127836562 1104 72-73 0.4730617179915342 1104 74-75 0.4293271857696297 1104 76-77 0.3903079926761528 1104 78-79 0.45860013977714686 1104 80-81 0.4335070758409074 1104 82-83 0.4123203075011901 1104 84-85 0.43634750407655787 1104 86-87 0.4466385561354045 1104 88-89 0.4288153382716047 1104 90-91 0.4402401324731784 1104 92-93 0.44187649101418813 1104 94-95 0.41836909105987985 1104 96-97 0.4487890094234359 1104 98-99 0.47544348991592855 1104 100 0.3759548474953469 1105 1 1.5710744720872931 1105 2 1.0423221269750016 1105 3 0.6030919109317665 1105 4 0.49960558471542527 1105 5 0.3393662217641449 1105 6 0.27263873349969714 1105 7 0.21303346307360016 1105 8 0.27703388664728834 1105 9 0.3294978531225965 1105 10-11 0.3129018652092199 1105 12-13 0.2773330063316166 1105 14-15 0.393744973108511 1105 16-17 0.31928345686954884 1105 18-19 0.318001633873628 1105 20-21 0.29527251166700097 1105 22-23 0.2933644814584042 1105 24-25 0.34586528430431684 1105 26-27 0.3392502538103841 1105 28-29 0.36633056969392186 1105 30-31 0.34249011288005704 1105 32-33 0.38520231796017157 1105 34-35 0.37659219114419074 1105 36-37 0.3454139643442673 1105 38-39 0.40031754459207036 1105 40-41 0.3788145979768913 1105 42-43 0.36614888241322774 1105 44-45 0.39168156060180337 1105 46-47 0.3965918155886925 1105 48-49 0.38805845556984764 1105 50-51 0.37798706608834465 1105 52-53 0.3122641009692231 1105 54-55 0.36307607131190167 1105 56-57 0.3438596544241008 1105 58-59 0.32507387554827005 1105 60-61 0.30685083383912826 1105 62-63 0.32288128688551865 1105 64-65 0.2988649189059345 1105 66-67 0.3350877480484229 1105 68-69 0.3844056523935251 1105 70-71 0.36286945799692205 1105 72-73 0.27896189363892887 1105 74-75 0.2179341663479235 1105 76-77 0.21838137201110897 1105 78-79 0.31302175854667524 1105 80-81 0.2822346539946494 1105 82-83 0.3009947572769178 1105 84-85 0.344156302034925 1105 86-87 0.3217406270704757 1105 88-89 0.35958593523379534 1105 90-91 0.35635759873287043 1105 92-93 0.3271762531910696 1105 94-95 0.30355568592471727 1105 96-97 0.283125198534254 1105 98-99 0.299601654453042 1105 100 0.2737073659101732 1106 1 1.4054093962782552 1106 2 0.8798005502076123 1106 3 0.47782960081706705 1106 4 0.43340656456290105 1106 5 0.25509360467614073 1106 6 0.2191147953110999 1106 7 0.18660548549749478 1106 8 0.18945790568306364 1106 9 0.2519189350971871 1106 10-11 0.21427181293029207 1106 12-13 0.20561299069034789 1106 14-15 0.33740697641690076 1106 16-17 0.26131035950917436 1106 18-19 0.19467370360513314 1106 20-21 0.209427297032299 1106 22-23 0.2046769904679877 1106 24-25 0.22728529357899419 1106 26-27 0.2564901328675475 1106 28-29 0.2810806175128491 1106 30-31 0.2843571428999567 1106 32-33 0.2510205294489012 1106 34-35 0.26141321016578445 1106 36-37 0.2923574418955539 1106 38-39 0.34440001915183416 1106 40-41 0.33016721844388286 1106 42-43 0.31331225502206195 1106 44-45 0.2902247505627713 1106 46-47 0.343782663123946 1106 48-49 0.37692459753148455 1106 50-51 0.3251652215955758 1106 52-53 0.2417438779069485 1106 54-55 0.2836736490708276 1106 56-57 0.28458648111452334 1106 58-59 0.21916441080257698 1106 60-61 0.24196455664011296 1106 62-63 0.2620059858217303 1106 64-65 0.2829570949930087 1106 66-67 0.256139787604333 1106 68-69 0.25034654014049806 1106 70-71 0.28886132616830196 1106 72-73 0.2506388195558884 1106 74-75 0.22445927063605353 1106 76-77 0.14271883065949353 1106 78-79 0.28814107842969605 1106 80-81 0.2584454882876841 1106 82-83 0.27496666255201063 1106 84-85 0.27565735060920105 1106 86-87 0.26452307684063925 1106 88-89 0.27522942808902684 1106 90-91 0.3113768718031906 1106 92-93 0.2612681287155283 1106 94-95 0.2571185270921319 1106 96-97 0.2724534573251951 1106 98-99 0.31619621584229307 1106 100 0.2675517923757269 1107 1 1.5747037455548032 1107 2 1.0512470376250747 1107 3 0.6042338055545571 1107 4 0.509514558070471 1107 5 0.33890621725654313 1107 6 0.32155258577855506 1107 7 0.27312127402436204 1107 8 0.2979360025429898 1107 9 0.36285794368147606 1107 10-11 0.3112376807677535 1107 12-13 0.30400600293840796 1107 14-15 0.4191420438798872 1107 16-17 0.37897737677510435 1107 18-19 0.3342069424734291 1107 20-21 0.3775686928673778 1107 22-23 0.38120721064172614 1107 24-25 0.4183683986581741 1107 26-27 0.3612843333873528 1107 28-29 0.33977743490069656 1107 30-31 0.3854723762656036 1107 32-33 0.3819988581873375 1107 34-35 0.42456496103235253 1107 36-37 0.4673477920379483 1107 38-39 0.46486581231739166 1107 40-41 0.4635763694067947 1107 42-43 0.4150589571667851 1107 44-45 0.3925336818891836 1107 46-47 0.4297521187980564 1107 48-49 0.43894872474121627 1107 50-51 0.4427640280323786 1107 52-53 0.43700646956459366 1107 54-55 0.4349726959444169 1107 56-57 0.42774638334137904 1107 58-59 0.40999855621493 1107 60-61 0.4081905166722848 1107 62-63 0.46621007066636366 1107 64-65 0.4017194929886898 1107 66-67 0.39477510952959705 1107 68-69 0.36734543101111683 1107 70-71 0.3995809563989212 1107 72-73 0.4054796096438835 1107 74-75 0.38687190727994647 1107 76-77 0.3097380791977038 1107 78-79 0.4197345328421349 1107 80-81 0.384358377302064 1107 82-83 0.3739215231234141 1107 84-85 0.40136315803670897 1107 86-87 0.36546956009682674 1107 88-89 0.38165876844098534 1107 90-91 0.4131158457911255 1107 92-93 0.4193873739641667 1107 94-95 0.4011354907529281 1107 96-97 0.37444193353408295 1107 98-99 0.42488529527018315 1107 100 0.3603785141962703 1108 1 1.3816397064996089 1108 2 0.8748235314140516 1108 3 0.44546801229413546 1108 4 0.37120082690267253 1108 5 0.19357655814640395 1108 6 0.16529919923451075 1108 7 0.12556084806442414 1108 8 0.12355568189418165 1108 9 0.16068208192515954 1108 10-11 0.10836458977929198 1108 12-13 0.10377177396926385 1108 14-15 0.1491686062424833 1108 16-17 0.11329026823924693 1108 18-19 0.08538630808306635 1108 20-21 0.09778767614140094 1108 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#Quality Count 6 44.0 7 792.0 8 1030.0 9 410.0 10 214.0 11 190.0 12 361.0 13 1641.0 14 7144.0 15 19006.0 16 31166.0 17 40934.0 18 48957.0 19 60992.0 20 81936.0 21 119240.0 22 181442.0 23 287437.0 24 436503.0 25 522642.0 26 513827.0 27 459804.0 28 447385.0 29 489280.0 30 573799.0 31 694207.0 32 848850.0 33 1071327.0 34 1435273.0 35 2098187.0 36 3509954.0 37 6543395.0 38 8067354.0 39 2181518.0 40 5181.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.94065997063931 33.98323479280504 27.546862321348236 19.529242915207405 2 21.88062656754454 32.876382384153665 26.32000561897368 18.92298542932812 3 26.1011593291564 26.397643357737017 26.30079598012074 21.20040133298585 4 20.990911336064983 26.934181273366775 25.84735364077722 26.227553749791028 5 21.163430718013622 32.27527502598815 25.814918260192073 20.746375995806158 6 26.949190326554763 26.37534094428776 25.878092961397297 20.797375767760176 7 20.91189289435686 26.472367001108655 31.807344702918535 20.808395401615947 8 26.57376907408631 26.53156504595532 25.984988607738785 20.909677272219586 9 26.569311840109272 26.248787336725382 26.200287303166174 20.981613519999172 10-11 20.565781528871536 29.6558813949531 29.04974468041145 20.72859239576391 12-13 20.598569162919116 26.665135873190003 28.97303932222495 23.763255641665936 14-15 20.62303034603145 32.55774538291311 25.973127557264895 20.846096713790548 16-17 23.695165869854875 26.5477842446655 25.95039144065534 23.806658444824283 18-19 20.738669829096132 29.545220785618636 25.87337651135923 23.842732873925996 20-21 23.71666157999277 29.544803958147625 25.890778078184045 20.847756383675563 22-23 23.727425588070624 26.622467279126997 28.847686114046322 20.802421018756053 24-25 20.84800167583126 29.522182770216908 25.739672036859453 23.89014351709238 26-27 20.902737070962804 26.560727916772287 31.659010829878632 20.877524182386278 28-29 20.88171235234032 26.630228454033084 31.64077507530354 20.84728411832306 30-31 20.897756120558693 26.61434224838606 31.611734831483744 20.876166799571507 32-33 20.90201680741065 26.631446721337305 31.579038161394884 20.88749830985716 34-35 20.97350635717869 26.648902055272174 31.54433216243226 20.833259425116875 36-37 21.008746769398762 26.624091635532626 31.464641237172213 20.902520357896396 38-39 21.037260721743134 26.645386948010398 31.421277418567602 20.89607491167887 40-41 21.040371301100027 26.725133147906742 31.416581898694496 20.817913652298735 42-43 21.062049676778187 26.7720698861243 31.328807325531283 20.83707311156623 44-45 21.11239640396205 26.81005359992318 31.253438769406184 20.824111226708585 46-47 21.17606788201894 26.905034608196132 31.134756305329773 20.784141204455153 48-49 21.233492419902124 26.928545218005883 31.012579702338478 20.825382659753515 50-51 21.22148385477448 27.021943625606383 30.912644321630108 20.843928197989033 52-53 21.31986218508733 27.19173721290482 30.642103370670874 20.846297231336976 54-55 21.665559423745073 27.4135528190447 29.908144400720545 21.01274335648968 56-57 22.041607978177822 27.89042188268124 28.815661422030693 21.252308717110242 58-59 22.157718105979026 28.371419413768283 28.146090244333173 21.32477223591952 60-61 22.229565235753647 28.62819557467248 27.719911456089264 21.422327733484618 62-63 22.240273381197504 28.759748823812632 27.50091155058983 21.499066244400037 64-65 22.184002180277204 28.907999559604274 27.392232265406193 21.51576599471233 66-67 22.184593912569547 28.784441862685956 27.371402591871757 21.65956163287274 68-69 22.118578523287727 28.678503935040727 27.451698934842067 21.751218606829475 70-71 22.072487674979683 28.60468509309942 27.52091465438353 21.801912577537365 72-73 22.019858161097343 28.53400781763519 27.553347559078357 21.89278646218911 74-75 22.043948502176473 28.56527401518717 27.41545430142787 21.975323181208484 76-77 22.071517230978692 28.643707709248396 27.261210007606724 22.023565052166187 78-79 22.16708689861222 28.75278157258187 26.907682386422977 22.172449142382938 80-81 22.19817603694125 28.91979722365596 26.565943426014694 22.3160833133881 82-83 22.189517500668472 29.081058549126336 26.270673518500047 22.45875043170514 84-85 22.216106373724507 29.12624521274446 26.042312769917654 22.615335643613378 86-87 22.254749257396288 29.12216250593841 25.864192675705688 22.75889556095962 88-89 22.354301909379018 29.151598425624325 25.677286644801818 22.81681302019484 90-91 22.353506465895066 29.122473509447083 25.557525796587953 22.966494228069894 92-93 22.378713267225837 29.090290678928117 25.504900280676722 23.02609577316932 94-95 22.390064719315177 29.090260069569773 25.46954397589911 23.050131235215936 96-97 22.42849738621381 29.01476977716369 25.431631369514935 23.125101467107566 98-99 22.410610540567486 28.949284330537477 25.441641931034198 23.19846319786084 100 22.38943661767611 28.946810301685478 25.435114668972354 23.228638411666054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 54811.0 1 33995.5 2 10261.0 3 6873.0 4 6319.5 5 6358.5 6 6605.5 7 6849.5 8 7174.0 9 7643.0 10 8334.5 11 10897.0 12 95421.0 13 118297.0 14 57813.0 15 61471.0 16 70836.5 17 80435.5 18 90284.0 19 100322.0 20 112056.0 21 128088.0 22 148778.5 23 173271.0 24 199431.0 25 228499.5 26 263886.0 27 305608.0 28 354275.0 29 402656.0 30 446986.0 31 493798.0 32 542290.0 33 586664.0 34 630682.5 35 681426.0 36 735199.0 37 784548.5 38 832029.5 39 871474.5 40 902153.5 41 941606.5 42 986989.5 43 1010729.0 44 1025461.0 45 1051667.5 46 1075175.5 47 1090406.0 48 1098530.5 49 1100500.5 50 1099074.5 51 1092953.0 52 1074454.0 53 1029707.0 54 964053.5 55 892741.5 56 815513.0 57 729658.5 58 637499.5 59 542158.5 60 444502.0 61 355481.5 62 277247.5 63 208445.0 64 154109.0 65 113648.0 66 82432.5 67 60271.5 68 44915.5 69 33652.5 70 25753.0 71 19764.5 72 15841.0 73 12602.0 74 9739.0 75 8098.5 76 6337.0 77 4089.5 78 2522.5 79 1669.0 80 1158.0 81 872.5 82 602.0 83 414.5 84 315.0 85 214.0 86 126.5 87 80.5 88 62.5 89 48.0 90 39.0 91 28.0 92 17.0 93 15.5 94 12.0 95 8.0 96 5.5 97 3.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10442987331774341 2 0.0 3 0.0 4 0.0 5 0.009638930910989102 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.796910747008375E-5 20-21 0.010680143366995846 22-23 0.0 24-25 9.09639587150977E-4 26-27 0.004968906244812212 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0074882830299392925 42-43 0.0389683101709856 44-45 0.014961946852227943 46-47 0.00464565932009249 48-49 0.0015821231390804494 50-51 0.0 52-53 0.0013904490832164934 54-55 3.4111484518161634E-5 56-57 8.381679053034002E-4 58-59 3.248712811253489E-5 60-61 2.7451623255091986E-4 62-63 2.6152138130590587E-4 64-65 4.873069216880234E-5 66-67 2.4852653006089193E-4 68-69 5.360376138568258E-4 70-71 4.353275167079676E-4 72-73 0.0012166429478144317 74-75 0.0016552191773336526 76-77 0.0024560268853076376 78-79 0.004536827440915498 80-81 0.0042330727930632965 82-83 0.007511024019618067 84-85 0.002397550054705075 86-87 0.002836126284224296 88-89 0.005617024450657283 90-91 0.005750221675918676 92-93 0.005540679699592825 94-95 0.0026379548027378333 96-97 0.005662506430014831 98-99 0.0079382297542979 100 0.29546718147069356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0781422E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.369994110745694 #Duplication Level Percentage of deduplicated Percentage of total 1 57.31963857244806 22.566738330273516 2 21.188136738320885 16.683536372107355 3 9.373959250733687 11.071581614872663 4 4.329171070822417 6.817577582507567 5 2.2315452823466675 4.392796231192532 6 1.2913161942724996 3.0503466578171308 7 0.8465658238437144 2.3330504049359932 8 0.6072062708799078 1.9124565846839854 9 0.4161531317494211 1.4745551711528777 >10 2.1575970665305033 14.817489921924052 >50 0.16000087092466272 4.4814167351129255 >100 0.07780383315643317 4.364553475187917 >500 3.558721851289305E-4 0.09628889031745436 >1k 5.091069685161786E-4 0.4257214235318333 >5k 8.182963489553996E-6 0.020038552557194087 >10k+ 3.2731853947822416E-5 5.491852051824883 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1572931 5.110001090917763 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 87484 0.28421039157970024 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.005464334948528369 1.9492276867520937E-5 0.0 6.497425622506979E-6 1.2994851245013957E-5 2 0.0055065682150746646 2.2740989678774424E-5 0.0 6.497425622506979E-6 1.9492276867520937E-5 3 0.005529309204753439 2.5989702490027915E-5 0.0 6.497425622506979E-6 1.9492276867520937E-5 4 0.005561796332865973 2.9238415301281402E-5 0.0 6.497425622506979E-6 1.9492276867520937E-5 5 0.005594283460978508 2.9238415301281402E-5 0.0 1.2994851245013957E-5 1.9492276867520937E-5 6 0.0056072783122235225 4.548197935754885E-5 0.0 1.2994851245013957E-5 2.5989702490027915E-5 7 0.005617024450657283 6.497425622506979E-5 0.0 1.2994851245013957E-5 2.5989702490027915E-5 8 0.00563326801471355 6.497425622506979E-5 0.0 1.2994851245013957E-5 2.5989702490027915E-5 9 0.005649511578769818 7.796910747008375E-5 0.0 1.9492276867520937E-5 2.5989702490027915E-5 10-11 0.005690120488910487 8.284217668696399E-5 0.0 1.9492276867520937E-5 2.5989702490027915E-5 12-13 0.005720983260617395 9.09639587150977E-5 0.0 2.5989702490027915E-5 2.9238415301281402E-5 14-15 0.005826566426983133 1.023344535544849E-4 0.0 3.086277170690815E-5 3.248712811253489E-5 16-17 0.005911032960075724 1.1208059198824537E-4 0.0 3.573584092378838E-5 3.248712811253489E-5 18-19 0.006000372562385194 1.1695366120512561E-4 0.0 3.573584092378838E-5 3.4111484518161634E-5 20-21 0.0060978339467227995 1.1695366120512561E-4 0.0 3.736019732941513E-5 4.223326654629536E-5 22-23 0.0063284925563217975 1.1695366120512561E-4 0.0 3.8984553735041874E-5 4.38576229519221E-5 24-25 0.007307979468914724 1.2182673042200585E-4 0.0 4.0608910140668616E-5 4.71063357631756E-5 26-27 0.010662275446533951 1.348215816670198E-4 0.0 5.035504857442908E-5 4.873069216880234E-5 28-29 0.020185877052723556 1.3969465088390005E-4 0.0 5.8476830602562804E-5 5.197940498005583E-5 30-31 0.042904125741819205 1.4619207650640702E-4 0.0 6.17255434138163E-5 5.197940498005583E-5 32-33 0.08639139543325841 1.7380613540206167E-4 0.0 6.659861263069654E-5 5.197940498005583E-5 34-35 0.16006245585405376 1.7705484821331518E-4 0.0 7.147168184757677E-5 5.197940498005583E-5 36-37 0.2741994830518226 1.8355227383582215E-4 0.0 7.472039465883026E-5 5.197940498005583E-5 38-39 0.43065749204179066 1.9004969945832912E-4 0.0 8.446653309259072E-5 5.197940498005583E-5 40-41 0.6377320709874936 1.9167405586395587E-4 0.0 9.09639587150977E-5 5.197940498005583E-5 42-43 0.890186294837191 1.9167405586395587E-4 0.0 9.09639587150977E-5 5.197940498005583E-5 44-45 1.1932603373554347 1.9492276867520936E-4 0.0 9.746138433760468E-5 5.360376138568257E-5 46-47 1.5471653648749562 2.0142019429771632E-4 0.0 1.0233445355448492E-4 5.522811779130931E-5 48-49 1.9537222809264625 2.1603940194835704E-4 0.0 1.0395880996011166E-4 5.522811779130931E-5 50-51 2.415045997550081 2.2416118397649076E-4 0.0 1.0395880996011166E-4 5.522811779130931E-5 52-53 2.9186322191352954 2.3390732241025122E-4 0.0 1.0883187917699188E-4 5.8476830602562804E-5 54-55 3.4687595004545275 2.5015088646651866E-4 0.0 1.1695366120512561E-4 6.17255434138163E-5 56-57 4.059970003984871 2.566483120890257E-4 0.0 1.234510868276326E-4 6.17255434138163E-5 58-59 4.697602989231621 2.712675197396664E-4 0.0 1.234510868276326E-4 7.472039465883026E-5 60-61 5.375705189968157 2.8426237098468034E-4 0.0 1.234510868276326E-4 7.796910747008375E-5 62-63 6.083058800857218 2.956328658240675E-4 0.0 1.2994851245013957E-4 7.796910747008375E-5 64-65 6.817362108872033 3.1837385550284196E-4 0.0 1.2994851245013957E-4 8.121782028133723E-5 66-67 7.583091840266508 3.248712811253489E-4 0.0 1.2994851245013957E-4 8.609088949821747E-5 68-69 8.37979317524707 3.5410969642663036E-4 0.0 1.315728688557663E-4 9.42126715263512E-5 70-71 9.206652311254498 3.573584092378838E-4 0.0 1.3319722526139305E-4 1.0720752277136514E-4 72-73 10.052542406910245 3.8659682453916525E-4 0.0 1.3319722526139305E-4 1.0720752277136514E-4 74-75 10.917802952703095 3.898455373504187E-4 0.0 1.4131900728952678E-4 1.0720752277136514E-4 76-77 11.802747449419329 3.979673193785524E-4 0.0 1.494407893176605E-4 1.0720752277136514E-4 78-79 12.70534707590832 4.0284038859543265E-4 0.0 1.559382149401675E-4 1.0720752277136514E-4 80-81 13.626870454522862 4.125865270291931E-4 3.2487128112534893E-6 1.559382149401675E-4 1.0720752277136514E-4 82-83 14.559613262831068 4.2558137827420706E-4 6.497425622506979E-6 1.559382149401675E-4 1.0883187917699188E-4 84-85 15.498323956573547 4.3857622951922105E-4 6.497425622506979E-6 1.6081128415704773E-4 1.1045623558261863E-4 86-87 16.4486033166369 4.45073655141728E-4 6.497425622506979E-6 1.6243564056267446E-4 1.1045623558261863E-4 88 17.17053552626646 4.45073655141728E-4 6.497425622506979E-6 1.6568435337392795E-4 1.1045623558261863E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 199835 0.0 85.39814 8 AGTGGTA 201845 0.0 84.92304 5 GTGGTAT 202615 0.0 84.54418 6 GTATCAA 201980 0.0 84.34698 9 TGGTATC 202545 0.0 84.29034 7 GCAGTGG 211835 0.0 80.99577 3 AAGCAGT 207915 0.0 80.84248 1 CAGTGGT 214040 0.0 79.96156 4 AGCAGTG 219110 0.0 78.20356 2 CAACGCA 195685 0.0 44.29271 12-13 ATCAACG 196895 0.0 44.09562 10-11 ACGCAGA 198650 0.0 43.748177 14-15 TCAACGC 195125 0.0 43.658783 12-13 GAGTACT 193410 0.0 43.516693 20-21 AACGCAG 196570 0.0 43.386105 14-15 AGAGTAC 199750 0.0 43.26451 18-19 CGCAGAG 200660 0.0 42.648186 16-17 AGTACTT 202800 0.0 42.267815 20-21 TATCAAC 202580 0.0 42.06598 10-11 GTACTTT 202610 0.0 41.528557 22-23 >>END_MODULE