FastQCFastQC Report
Thu 26 May 2016
SRR1660761_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660761_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30558859
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14856814.8617031152897425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1215960.39790752658664386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1864500.086.426528
GTGGTAT1890600.085.2284246
TGGTATC1889900.085.041277
GTATCAA1894500.084.899259
AGTGGTA1901850.084.74655
AAGCAGT1994600.081.157991
GCAGTGG2001300.081.092973
CAGTGGT1997900.080.587624
AGCAGTG2077650.078.148772
CAACGCA1831750.044.74106612-13
ATCAACG1850450.044.4158510-11
TCAACGC1827450.044.18190812-13
ACGCAGA1861750.044.03679714-15
AACGCAG1847600.043.77256814-15
AGAGTAC1854000.043.74612818-19
GAGTACT1813200.043.62838720-21
CGCAGAG1878050.043.01292416-17
TATCAAC1894900.042.46049510-11
AGTACTT1912050.042.02095420-21
GTACTTT1907200.041.47272522-23
Produced by FastQC (version 0.11.5)