##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660761_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30558859 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.676980478885028 33.0 31.0 34.0 30.0 34.0 2 31.83257571887746 34.0 31.0 34.0 30.0 34.0 3 31.874421129401462 34.0 31.0 34.0 30.0 34.0 4 35.29762122335785 37.0 35.0 37.0 33.0 37.0 5 35.29214078313591 37.0 35.0 37.0 33.0 37.0 6 35.30021487386031 37.0 35.0 37.0 32.0 37.0 7 35.283051602155695 37.0 35.0 37.0 32.0 37.0 8 35.283697077826105 37.0 35.0 37.0 32.0 37.0 9 36.891557731262154 39.0 37.0 39.0 33.0 39.0 10-11 36.834187493715 39.0 37.0 39.0 32.5 39.0 12-13 36.85790982902863 39.0 37.0 39.0 33.0 39.0 14-15 38.13704225671515 40.0 38.0 41.0 33.0 41.0 16-17 38.10301803153057 40.0 38.0 41.0 33.0 41.0 18-19 38.04916602089103 40.0 38.0 41.0 32.5 41.0 20-21 38.003350255976514 40.0 38.0 41.0 32.0 41.0 22-23 37.87287830347331 40.0 37.5 41.0 32.5 41.0 24-25 37.79649763755904 40.0 38.0 41.0 32.0 41.0 26-27 37.76183547952494 40.0 38.0 41.0 32.0 41.0 28-29 37.666908996831324 40.0 37.5 41.0 32.0 41.0 30-31 37.542902239903654 40.0 37.0 41.0 31.0 41.0 32-33 37.40963054281575 40.0 37.0 41.0 31.0 41.0 34-35 37.27038488249839 40.0 37.0 41.0 30.5 41.0 36-37 37.06523041321667 39.5 36.5 41.0 30.0 41.0 38-39 36.63325382992866 39.0 35.0 41.0 30.0 41.0 40-41 36.609675004554326 39.0 35.0 41.0 30.0 41.0 42-43 36.62200426069573 39.0 35.0 41.0 30.0 41.0 44-45 36.55407518651138 39.0 35.0 41.0 30.0 41.0 46-47 36.676876106532646 39.0 35.0 41.0 30.0 41.0 48-49 36.60982237589434 39.0 35.0 41.0 30.0 41.0 50-51 35.48291505255481 38.0 34.0 40.0 27.5 40.5 52-53 35.52104882253621 38.5 34.5 39.5 27.5 40.5 54-55 35.8030441679776 39.0 35.0 41.0 26.5 41.0 56-57 35.48109366583353 39.0 35.0 41.0 24.5 41.0 58-59 35.17546321019381 39.0 34.5 41.0 23.0 41.0 60-61 34.93632803829489 38.5 34.0 41.0 23.0 41.0 62-63 34.67131462598129 38.0 34.0 40.0 23.0 41.0 64-65 34.387520031425254 37.0 34.0 40.0 22.0 41.0 66-67 34.10189298952555 37.0 33.0 40.0 22.0 41.0 68-69 33.804456164413736 36.0 33.0 39.0 22.0 41.0 70-71 33.482964890803025 35.5 33.0 39.0 22.0 41.0 72-73 33.141220472269595 35.0 32.5 39.0 21.5 40.5 74-75 32.78411029678824 35.0 32.0 37.5 20.5 40.0 76-77 32.40183944695056 35.0 32.0 37.0 20.0 39.0 78-79 32.05015491579709 35.0 32.0 36.5 20.0 39.0 80-81 31.724282146790884 35.0 31.0 36.0 19.0 38.5 82-83 31.42347903107246 35.0 31.0 35.5 18.5 37.0 84-85 31.164699375719493 35.0 31.0 35.0 18.0 37.0 86-87 30.918030300149624 35.0 31.0 35.0 17.5 36.5 88-89 30.730587061512995 35.0 30.5 35.0 17.0 36.0 90-91 30.561347545731337 34.5 30.0 35.0 17.0 36.0 92-93 30.39812523759477 34.0 30.0 35.0 15.5 35.5 94-95 30.23449075765558 34.0 30.0 35.0 15.0 35.0 96-97 30.11671150745517 34.0 30.0 35.0 15.0 35.0 98-99 30.006318756861965 34.0 29.5 35.0 14.0 35.0 100 29.87694684543032 34.0 29.0 35.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6978318789004057 1101 2 0.6776926860711576 1101 3 0.6885229597731772 1101 4 0.6149359371042067 1101 5 0.6283315714262727 1101 6 0.6374004234638875 1101 7 0.587824958370895 1101 8 0.6269399050629758 1101 9 0.7919850176374297 1101 10-11 0.7377489958861858 1101 12-13 0.7051894831388879 1101 14-15 0.8776152988444963 1101 16-17 0.8624823244487416 1101 18-19 0.859467210591653 1101 20-21 0.7839271498889602 1101 22-23 0.7747157227084287 1101 24-25 0.6928720987644112 1101 26-27 0.6995765337499478 1101 28-29 0.6548961415222081 1101 30-31 0.7173389900817995 1101 32-33 0.750040876101842 1101 34-35 0.714147162540506 1101 36-37 0.729920475079922 1101 38-39 0.7729084117712404 1101 40-41 0.7352401448403967 1101 42-43 0.6878158303232098 1101 44-45 0.6725145851043024 1101 46-47 0.7622795522620649 1101 48-49 0.7618854828278216 1101 50-51 0.7719231340355606 1101 52-53 0.822340836943404 1101 54-55 0.8526831302817115 1101 56-57 0.8017250963768277 1101 58-59 0.865195146441927 1101 60-61 0.811801817956038 1101 62-63 0.7543160573621748 1101 64-65 0.6828937534878676 1101 66-67 0.6683959251514153 1101 68-69 0.6415252486788035 1101 70-71 0.6371948754063581 1101 72-73 0.6001219464868086 1101 74-75 0.572045142768836 1101 76-77 0.5296610537047002 1101 78-79 0.46401733003317247 1101 80-81 0.43959806650800815 1101 82-83 0.4074226046529681 1101 84-85 0.40165531419378553 1101 86-87 0.31470566963852065 1101 88-89 0.32825473875382016 1101 90-91 0.31107520045535964 1101 92-93 0.2718288838984755 1101 94-95 0.28045587839671526 1101 96-97 0.24318983722535492 1101 98-99 0.20551213455390638 1101 100 0.19615140744435067 1102 1 0.6002591253372422 1102 2 0.6107377810277548 1102 3 0.5994154591566065 1102 4 0.5439894777384566 1102 5 0.5147282417744847 1102 6 0.5773074960415627 1102 7 0.5604759367942762 1102 8 0.5916811249134781 1102 9 0.699718545758806 1102 10-11 0.656239843572564 1102 12-13 0.6422614101984578 1102 14-15 0.7755592675358258 1102 16-17 0.7731856833454955 1102 18-19 0.7480017096442566 1102 20-21 0.7210135742437558 1102 22-23 0.6839388559741693 1102 24-25 0.722902355823372 1102 26-27 0.7019883157192695 1102 28-29 0.7412463382131591 1102 30-31 0.7241761785970127 1102 32-33 0.6997327689653545 1102 34-35 0.6615045429550506 1102 36-37 0.6594986566060186 1102 38-39 0.7395366773538754 1102 40-41 0.6748374822082326 1102 42-43 0.6537200995417152 1102 44-45 0.6368930625302411 1102 46-47 0.7232408769286636 1102 48-49 0.7336529473098778 1102 50-51 0.6682368576992772 1102 52-53 0.7403786782793347 1102 54-55 0.8278488396478849 1102 56-57 0.803520192833922 1102 58-59 0.8013528288611695 1102 60-61 0.7774480030804227 1102 62-63 0.7382954891059228 1102 64-65 0.6809562371389717 1102 66-67 0.6606552872848255 1102 68-69 0.6868182320873686 1102 70-71 0.6144526367324588 1102 72-73 0.6452388784736414 1102 74-75 0.6085904650966683 1102 76-77 0.5426673732838694 1102 78-79 0.471688579118684 1102 80-81 0.49066893098134656 1102 82-83 0.5196669118237303 1102 84-85 0.4649454113819118 1102 86-87 0.4144879577850027 1102 88-89 0.38971553245312407 1102 90-91 0.3411142382039891 1102 92-93 0.29652784792319764 1102 94-95 0.32387298314041146 1102 96-97 0.2682084215094278 1102 98-99 0.257150810785884 1102 100 0.27897846026838735 1103 1 0.6041039766093057 1103 2 0.5755982347871473 1103 3 0.5767039479707812 1103 4 0.5169597616393773 1103 5 0.4890626767144539 1103 6 0.532511975379542 1103 7 0.5056615970955818 1103 8 0.5207205577241396 1103 9 0.6297475363700116 1103 10-11 0.6126901608708124 1103 12-13 0.6044043453076071 1103 14-15 0.7491447322908797 1103 16-17 0.7099803058431888 1103 18-19 0.7091893773700946 1103 20-21 0.6631597784164214 1103 22-23 0.6919817524787604 1103 24-25 0.6802961535596665 1103 26-27 0.7197525088086252 1103 28-29 0.6838399994321165 1103 30-31 0.6578526341267619 1103 32-33 0.6527159641407607 1103 34-35 0.6222061139924122 1103 36-37 0.6288416167349098 1103 38-39 0.6610083580438584 1103 40-41 0.6075793447618807 1103 42-43 0.6620745794334226 1103 44-45 0.6285083640987494 1103 46-47 0.6830372161556113 1103 48-49 0.6798506779770364 1103 50-51 0.6101270538635504 1103 52-53 0.6731481989419379 1103 54-55 0.7717437907959024 1103 56-57 0.7387422795181422 1103 58-59 0.7185631771197052 1103 60-61 0.6857980109867725 1103 62-63 0.6529951784019943 1103 64-65 0.621859836690966 1103 66-67 0.5766984044982877 1103 68-69 0.5754077649850942 1103 70-71 0.5806066660026232 1103 72-73 0.5693907060461001 1103 74-75 0.569062819767133 1103 76-77 0.5491832969130144 1103 78-79 0.5188952734696528 1103 80-81 0.4648126320113555 1103 82-83 0.39986537444155346 1103 84-85 0.40356668238510096 1103 86-87 0.36436923453064907 1103 88-89 0.37044032793611237 1103 90-91 0.3616980748900751 1103 92-93 0.31462808943963694 1103 94-95 0.28689214548397146 1103 96-97 0.2557575523740425 1103 98-99 0.2179548399764819 1103 100 0.21464452838617376 1104 1 0.5321526300130195 1104 2 0.5255524070083517 1104 3 0.5090402208124978 1104 4 0.4638570925753882 1104 5 0.5098701644561316 1104 6 0.5089269394139038 1104 7 0.44614582201366204 1104 8 0.502816631514527 1104 9 0.6095480418083881 1104 10-11 0.6081718875325066 1104 12-13 0.5854244204200327 1104 14-15 0.711450165131339 1104 16-17 0.7012838713546259 1104 18-19 0.7193973204334014 1104 20-21 0.6454322880791565 1104 22-23 0.5900132297217553 1104 24-25 0.6337346635699745 1104 26-27 0.6328229412042816 1104 28-29 0.6114369578052035 1104 30-31 0.6104626172743153 1104 32-33 0.6147691851160388 1104 34-35 0.6192808575457391 1104 36-37 0.6189338686840884 1104 38-39 0.6708873619377158 1104 40-41 0.6370162814595446 1104 42-43 0.6170863329965428 1104 44-45 0.6036063236232181 1104 46-47 0.6501008582465957 1104 48-49 0.6499575849892949 1104 50-51 0.5714371275681245 1104 52-53 0.620143547972873 1104 54-55 0.7249894719299235 1104 56-57 0.7748221367056018 1104 58-59 0.7871666691634829 1104 60-61 0.7942367771419754 1104 62-63 0.7485579072779984 1104 64-65 0.6999601377475457 1104 66-67 0.6434758291300113 1104 68-69 0.6590453840924653 1104 70-71 0.6536557502110298 1104 72-73 0.6455603626822324 1104 74-75 0.628828868019724 1104 76-77 0.5917608797617717 1104 78-79 0.5742519533703643 1104 80-81 0.5588383894465174 1104 82-83 0.5494840457674854 1104 84-85 0.5054078859205795 1104 86-87 0.5350601650979456 1104 88-89 0.5644979935559178 1104 90-91 0.537546116843604 1104 92-93 0.502625055523982 1104 94-95 0.4723134467982213 1104 96-97 0.4717973693513038 1104 98-99 0.4245079901683262 1104 100 0.3443495424519867 1105 1 0.43711469618449783 1105 2 0.42664955911485336 1105 3 0.4097596258771823 1105 4 0.3941634088249941 1105 5 0.4123882928082381 1105 6 0.4623357006721207 1105 7 0.40588113111411417 1105 8 0.4466537582035315 1105 9 0.5046413552543072 1105 10-11 0.5038288184260153 1105 12-13 0.4843389889555496 1105 14-15 0.5745768534323901 1105 16-17 0.5667568147004332 1105 18-19 0.5740885147915904 1105 20-21 0.5297000254012119 1105 22-23 0.5359491992710943 1105 24-25 0.5390050637175392 1105 26-27 0.5001666197528962 1105 28-29 0.502294688187753 1105 30-31 0.5291523677863736 1105 32-33 0.527161537366716 1105 34-35 0.5019716978352946 1105 36-37 0.5084152754742206 1105 38-39 0.5231971862062892 1105 40-41 0.46874577465383993 1105 42-43 0.46860532562073587 1105 44-45 0.4567262297080674 1105 46-47 0.5353169497745824 1105 48-49 0.5113117321391059 1105 50-51 0.46373574002566187 1105 52-53 0.4981174336989369 1105 54-55 0.5655588332768602 1105 56-57 0.5817409270502836 1105 58-59 0.5872784591054483 1105 60-61 0.5908476193633092 1105 62-63 0.5386549384037949 1105 64-65 0.4851921478240939 1105 66-67 0.47011663034645323 1105 68-69 0.5042227606422856 1105 70-71 0.5053225851065051 1105 72-73 0.502804959086113 1105 74-75 0.47015454047427596 1105 76-77 0.4546625654361236 1105 78-79 0.4335625876782885 1105 80-81 0.4653215640115427 1105 82-83 0.45827882744590553 1105 84-85 0.4288885749797764 1105 86-87 0.42307259166226885 1105 88-89 0.4030797954635936 1105 90-91 0.34662928012724237 1105 92-93 0.3846129409574992 1105 94-95 0.3887890453598395 1105 96-97 0.36853262580891055 1105 98-99 0.3169303775111487 1105 100 0.3349967174338033 1106 1 0.3354901045621155 1106 2 0.3080641432322615 1106 3 0.27740944190322026 1106 4 0.3194174053874761 1106 5 0.282567390460315 1106 6 0.3189526441775641 1106 7 0.26927983613531126 1106 8 0.2813662309220035 1106 9 0.3998088409818479 1106 10-11 0.3717314257261677 1106 12-13 0.3528549405219792 1106 14-15 0.40115195941827153 1106 16-17 0.4069855755063756 1106 18-19 0.3999995144001005 1106 20-21 0.40049627868315696 1106 22-23 0.3488540962104807 1106 24-25 0.3925585978969721 1106 26-27 0.3821295788354817 1106 28-29 0.360735630716043 1106 30-31 0.34878437894408165 1106 32-33 0.3419346099226246 1106 34-35 0.30561380166096086 1106 36-37 0.3551821958733967 1106 38-39 0.3529568773588565 1106 40-41 0.32951437936144856 1106 42-43 0.278931977461788 1106 44-45 0.18000634648175406 1106 46-47 0.26036364728366124 1106 48-49 0.3024710280330254 1106 50-51 0.287934916930368 1106 52-53 0.30531879639536186 1106 54-55 0.35624304069512647 1106 56-57 0.39817483451216873 1106 58-59 0.44529031422851517 1106 60-61 0.42836185140257044 1106 62-63 0.42838646469731145 1106 64-65 0.3532662712316892 1106 66-67 0.40004019642360333 1106 68-69 0.3469108117163344 1106 70-71 0.43974778183083885 1106 72-73 0.38832215920104574 1106 74-75 0.3968812588971318 1106 76-77 0.4112711073842732 1106 78-79 0.34737913768944395 1106 80-81 0.3028765077205584 1106 82-83 0.376167601151959 1106 84-85 0.33007521677471274 1106 86-87 0.3053213682121836 1106 88-89 0.2779478495414871 1106 90-91 0.3045090431327715 1106 92-93 0.3041096629448852 1106 94-95 0.30992783598052753 1106 96-97 0.2620781395674108 1106 98-99 0.24548338240776602 1106 100 0.26731003537646103 1107 1 0.42774576678874254 1107 2 0.4266688128069056 1107 3 0.35112417481553493 1107 4 0.35495035535341657 1107 5 0.39258642281854605 1107 6 0.43212317462059247 1107 7 0.38635431098150974 1107 8 0.40028713531746263 1107 9 0.5266699160769264 1107 10-11 0.4814999748803501 1107 12-13 0.4559857165786525 1107 14-15 0.5354239694322729 1107 16-17 0.5788866951364398 1107 18-19 0.5647862004538027 1107 20-21 0.5255864716245995 1107 22-23 0.505312704159131 1107 24-25 0.5523190445309893 1107 26-27 0.541827018193338 1107 28-29 0.5315001469129896 1107 30-31 0.5162372780770141 1107 32-33 0.5057801372270987 1107 34-35 0.49157698343085343 1107 36-37 0.49986042354201743 1107 38-39 0.565125161238015 1107 40-41 0.539297386465357 1107 42-43 0.5454299602545305 1107 44-45 0.5237526641606607 1107 46-47 0.537077971116851 1107 48-49 0.5468535863070088 1107 50-51 0.4749908296592267 1107 52-53 0.5305996408044322 1107 54-55 0.6025337786287892 1107 56-57 0.611956642219063 1107 58-59 0.6731496175246576 1107 60-61 0.6307019795064548 1107 62-63 0.6065315100200905 1107 64-65 0.5546068612243502 1107 66-67 0.5120602119593798 1107 68-69 0.586817199305834 1107 70-71 0.5650381860283247 1107 72-73 0.5446018222223401 1107 74-75 0.5464048382150182 1107 76-77 0.5538134202551461 1107 78-79 0.5570617661993964 1107 80-81 0.5477311203094892 1107 82-83 0.516023755400191 1107 84-85 0.5022354267353073 1107 86-87 0.496049131451592 1107 88-89 0.5385986446574869 1107 90-91 0.5381721580814833 1107 92-93 0.5250803695477195 1107 94-95 0.478376999264956 1107 96-97 0.5320359979799214 1107 98-99 0.5598350782416937 1107 100 0.5299519037022264 1108 1 0.1932465010570077 1108 2 0.15950346433314522 1108 3 0.2104019040866909 1108 4 0.2278725686927885 1108 5 0.19662180219140168 1108 6 0.21269534540778068 1108 7 0.20887266714380814 1108 8 0.23145040261076133 1108 9 0.2518829918874843 1108 10-11 0.22018893572166576 1108 12-13 0.19092478966056348 1108 14-15 0.2616894316255838 1108 16-17 0.2611555137107118 1108 18-19 0.2544369178505974 1108 20-21 0.24808721621037222 1108 22-23 0.19750612942487322 1108 24-25 0.2089657143114323 1108 26-27 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-1.6514964347411834 2316 78-79 -1.591354556945067 2316 80-81 -1.5699219476538318 2316 82-83 -1.7937312901907028 2316 84-85 -1.4912687050335478 2316 86-87 -1.421656201878477 2316 88-89 -1.575165447849301 2316 90-91 -1.4686537847733305 2316 92-93 -1.3918504119121415 2316 94-95 -1.3843691780294343 2316 96-97 -1.2603688471203789 2316 98-99 -1.2758276278110081 2316 100 -1.4370275537939037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 44.0 4 70.0 5 163.0 6 1624.0 7 18722.0 8 27213.0 9 34625.0 10 37341.0 11 39279.0 12 44648.0 13 52620.0 14 65569.0 15 83459.0 16 101750.0 17 120301.0 18 133385.0 19 146888.0 20 167015.0 21 201791.0 22 259266.0 23 355262.0 24 481969.0 25 563477.0 26 557088.0 27 499102.0 28 472287.0 29 500395.0 30 574269.0 31 687775.0 32 846540.0 33 1083476.0 34 1463542.0 35 2152162.0 36 3509384.0 37 6091644.0 38 7133047.0 39 2046628.0 40 5039.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.03464983408761 34.33554096966302 27.32443414552459 20.30537505072478 2 21.772534374202856 32.66437358036246 26.145058916778545 19.418033128656138 3 25.988465330081212 26.205673884196717 25.84597549754775 21.959885288174323 4 20.524133372274623 26.985576179712407 25.770797797362953 26.719492650650018 5 21.293996229813196 31.938670947302793 25.794803168642854 20.97252965424116 6 26.95856543596736 26.281279022884984 25.721748969750475 21.038406571397182 7 21.072753403522036 26.46587033894165 31.373380138309482 21.087996119226833 8 26.638376779708956 26.55450911959769 25.80341105013116 21.003703050562194 9 26.473059743493693 26.25868982870074 26.12007536014352 21.14817506766205 10-11 20.76352037643293 29.48995932668555 28.84246946801708 20.90405082886444 12-13 20.77681335255897 26.604503870916723 28.79217970481968 23.82650307170462 14-15 20.83323038991737 32.31917624934884 25.845912636986874 21.00168072374692 16-17 23.791475656862712 26.511199256490563 25.82368340388625 23.87364168276047 18-19 20.91452923684094 29.38721141388165 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21.3223244532758 26.82427888267691 30.887703322396533 20.965693341650756 46-47 21.360468259841227 26.90716005297577 30.833119367119448 20.899252320063553 48-49 21.475486006400658 26.92181862559839 30.673654926924304 20.929040441076644 50-51 21.543142270933615 26.94953280462461 30.592970650580924 20.91435427386085 52-53 21.69210638197967 27.09648950111849 30.362063880336354 20.84934023656548 54-55 22.102145349324687 27.298704239168316 29.645426943430525 20.953723468076472 56-57 22.591709811622753 27.709665763129145 28.620728675534057 21.077895749714052 58-59 22.802876020161523 28.17537289723729 28.004075686608765 21.017675395992423 60-61 22.98630978064308 28.405283182160225 27.616693699947803 20.991713337248896 62-63 23.099384503822545 28.550506616722277 27.447768695765575 20.902340183689603 64-65 23.248988171565323 28.635725023512173 27.389032034307903 20.726254770614602 66-67 23.405858374680445 28.456629866749388 27.501796548867336 20.63571520970283 68-69 23.54051089923185 28.262153158449838 27.673561602711906 20.52377433960641 70-71 23.66595004817789 28.13885192155742 27.82591456434141 20.369283465923278 72-73 23.86804068235042 28.032877710146437 27.82018178217936 20.278899825323784 74-75 24.082858841055256 28.05583702890042 27.697917304234416 20.163386825809905 76-77 24.39302464911267 28.14653496297655 27.41229973576123 20.04814065214955 78-79 24.680466043033398 28.202156048545675 27.0875076781044 20.029870230316533 80-81 24.953979562488165 28.28661564002639 26.77491062273596 19.98449417474948 82-83 25.12149981120363 28.39665852357506 26.54331293861109 19.93852872661022 84-85 25.297978625381578 28.383705213785554 26.392249881715113 19.926066279117755 86-87 25.48540195485321 28.341385311270344 26.271926984850147 19.9012857490263 88-89 25.64851924406602 28.281573433885637 26.18570834848104 19.884198973567297 90-91 25.813413039610435 28.212384128480693 26.05279970055467 19.9214031313542 92-93 25.938350225231034 28.218350841407595 25.9966741853631 19.846624747998273 94-95 26.063694181179248 28.26020659142967 25.913999760563577 19.762099466827504 96-97 26.124012512461924 28.269288967717966 25.81985952407684 19.786838995743274 98-99 26.185548000853405 28.295016147453527 25.74767659309316 19.77175925859991 100 26.208383651514488 28.421859773071755 25.636729824885364 19.733026750528392 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 65518.0 1 40669.5 2 13490.0 3 10808.0 4 10485.5 5 10511.5 6 10811.0 7 11199.5 8 11434.5 9 11603.5 10 12339.0 11 14837.0 12 96225.0 13 119351.5 14 62718.5 15 66689.5 16 75047.0 17 83662.5 18 91856.0 19 100828.5 20 111909.0 21 127342.0 22 148525.5 23 172051.5 24 196094.5 25 223780.5 26 253816.5 27 288688.5 28 337747.5 29 387341.0 30 427444.0 31 470653.0 32 519211.5 33 561069.0 34 599149.5 35 650489.5 36 705140.0 37 748415.5 38 798278.5 39 843572.0 40 870763.5 41 908637.5 42 958029.0 43 981127.5 44 984421.0 45 1006448.0 46 1038039.5 47 1060578.0 48 1075443.5 49 1077792.0 50 1072755.0 51 1066595.0 52 1050881.0 53 1013858.0 54 960085.0 55 901502.0 56 837571.5 57 765475.5 58 681278.0 59 586419.5 60 487866.5 61 397589.5 62 315518.5 63 241850.5 64 182493.5 65 135191.5 66 98782.0 67 73726.5 68 55969.5 69 42677.0 70 33429.0 71 26923.5 72 22306.5 73 18191.5 74 15041.0 75 13258.5 76 10619.0 77 7045.0 78 4786.5 79 3741.5 80 2871.5 81 2265.5 82 1831.0 83 1490.5 84 1265.5 85 1075.0 86 889.0 87 712.5 88 596.5 89 497.0 90 445.0 91 419.5 92 362.5 93 324.5 94 266.0 95 207.0 96 184.5 97 148.0 98 102.5 99 80.5 100 54.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04621900313751898 2 0.014414805212459013 3 0.00520634621862027 4 1.1126069857516605E-4 5 5.268521314882863E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.544746975009767E-6 12-13 4.0904668593811046E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0384323904240011 22-23 0.0756474579106504 24-25 0.031215170697309086 26-27 0.0356181492247469 28-29 0.04034345654070396 30-31 0.033792164818719175 32-33 0.034503906052251496 34-35 0.03550525233942799 36-37 0.0330656979044931 38-39 0.04351602263683994 40-41 0.06406489195162686 42-43 0.07232599882083293 44-45 0.07514842095380589 46-47 0.048125160693990574 48-49 0.030559059813064355 50-51 0.02807041977581689 52-53 0.028235674636935888 54-55 0.02909958123763718 56-57 0.029248474231318648 58-59 0.037200341805955515 60-61 0.05724362941692293 62-63 0.0806051037442203 64-65 0.10857735231541205 66-67 0.08967775923832759 68-69 0.05301899524455412 70-71 0.04662314126322583 72-73 0.050692337694938146 74-75 0.04816770154932813 76-77 0.051212645079451426 78-79 0.043838351425359176 80-81 0.04227742927181934 82-83 0.04162786313454962 84-85 0.049136324101629575 86-87 0.05287010225087266 88-89 0.058699835618862606 90-91 0.0558168745763708 92-93 0.051263366868507756 94-95 0.05335441352702337 96-97 0.055067501047732184 98-99 0.049702444714967924 100 0.05145480071752678 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0558859E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.24409241505618 #Duplication Level Percentage of deduplicated Percentage of total 1 59.13260518577036 20.84075001910321 2 18.81584223712862 13.262945653449574 3 8.978364043640626 9.493028762702632 4 4.212708675187183 5.938923754640239 5 2.2640325738328646 3.98968866314315 6 1.4028918857549264 2.966619076192744 7 0.9700574345443106 2.39321557096944 8 0.6663213909333707 1.8787114144147596 9 0.5295354281261707 1.679669600933259 >10 2.6798744840783697 16.960658952643453 >50 0.18683245331010406 4.544576295877816 >100 0.1595347160586779 10.070392798158414 >500 7.785134223656315E-4 0.17332839603699277 >1k 5.563238691874082E-4 0.40155176368671397 >5k 2.770900413037028E-5 0.054278244259936534 >10k+ 3.694533869826099E-5 5.351661033787809 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1485681 4.8617031152897425 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 121596 0.39790752658664386 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.008197295586199733 3.2723734875048836E-6 0.0 0.0 3.599610836255372E-5 2 0.008220202200612267 3.2723734875048836E-6 0.0 0.0 3.599610836255372E-5 3 0.008233291694562286 1.3089493950019534E-5 0.0 0.0 3.92684818500586E-5 4 0.008252925935487317 1.96342409250293E-5 0.0 0.0 6.544746975009768E-5 5 0.008262743055949831 3.599610836255372E-5 0.0 0.0 6.544746975009768E-5 6 0.008262743055949831 3.599610836255372E-5 0.0 0.0 6.544746975009768E-5 7 0.008262743055949831 4.254085533756349E-5 0.0 3.2723734875048836E-6 6.871984323760255E-5 8 0.008266015429437336 4.908560231257325E-5 0.0 3.2723734875048836E-6 6.871984323760255E-5 9 0.008272560176412347 5.235797580007814E-5 3.2723734875048836E-6 3.2723734875048836E-6 7.199221672510744E-5 10-11 0.00828237729687486 7.362840346885989E-5 3.2723734875048836E-6 3.2723734875048836E-6 7.690077695636476E-5 12-13 0.008303647724543641 9.489883113764162E-5 3.2723734875048836E-6 4.908560231257325E-6 8.508171067512698E-5 14-15 0.008328190525699929 1.0144357811265139E-4 3.2723734875048836E-6 6.544746975009767E-6 8.671789741887942E-5 16-17 0.008370731381037493 1.1289688531891849E-4 4.908560231257325E-6 1.3089493950019534E-5 8.835408416263186E-5 18-19 0.008424725543581323 1.210778190376807E-4 6.544746975009767E-6 1.3089493950019534E-5 9.81712046251465E-5 20-21 0.0084770835193814 1.2762256601269047E-4 6.544746975009767E-6 1.3089493950019534E-5 1.1126069857516605E-4 22-23 0.00870451347676299 1.4071205996270999E-4 6.544746975009767E-6 1.3089493950019534E-5 1.1289688531891849E-4 24-25 0.009892385052727264 1.4071205996270999E-4 6.544746975009767E-6 1.636186743752442E-5 1.1616925880642337E-4 26-27 0.014552244898934218 1.4562062019396731E-4 6.544746975009767E-6 1.636186743752442E-5 1.292587527564429E-4 28-29 0.027874077366566597 1.5052918042522464E-4 6.544746975009767E-6 1.636186743752442E-5 1.4234824670646243E-4 30-31 0.057318894007135544 1.5216536716897708E-4 6.544746975009767E-6 1.636186743752442E-5 1.488929936814722E-4 32-33 0.11269072578920569 1.570739274002344E-4 6.544746975009767E-6 1.636186743752442E-5 1.5052918042522464E-4 34-35 0.20348763676025994 1.7834435506901617E-4 6.544746975009767E-6 1.7998054181276862E-5 1.5380155391272953E-4 36-37 0.33698084080953417 1.881614755315308E-4 6.544746975009767E-6 1.96342409250293E-5 1.6198248763149174E-4 38-39 0.5176518534281663 1.8979766227528324E-4 6.544746975009767E-6 1.96342409250293E-5 1.685272346065015E-4 40-41 0.7473217504619528 2.077957164565601E-4 6.544746975009767E-6 1.96342409250293E-5 1.717996080940064E-4 42-43 1.0280226758466342 2.1597665017532232E-4 6.544746975009767E-6 2.1270427668781744E-5 1.7834435506901617E-4 44-45 1.358111898091483 2.2415758389408453E-4 6.544746975009767E-6 2.2906614412534187E-5 1.8652528878777835E-4 46-47 1.7405034657871226 2.3888326458785652E-4 6.544746975009767E-6 2.9451361387543954E-5 1.9143384901903568E-4 48-49 2.175537705776253 2.4870038505037117E-4 6.544746975009767E-6 2.9451361387543954E-5 1.9470622250654057E-4 50-51 2.6566698710838645 2.5197275853787606E-4 6.544746975009767E-6 2.9451361387543954E-5 2.0125096948155034E-4 52-53 3.17789679254713 2.6015369225663824E-4 6.544746975009767E-6 2.9451361387543954E-5 2.192490236628272E-4 54-55 3.737608462410197 2.650622524878956E-4 6.544746975009767E-6 3.599610836255372E-5 2.2088521040657965E-4 56-57 4.338578217203725 2.66698439231648E-4 6.544746975009767E-6 3.599610836255372E-5 2.2579377063783698E-4 58-59 4.974169683495054 2.8142411992541997E-4 6.544746975009767E-6 3.763229510630616E-5 2.307023308690943E-4 60-61 5.645186228975369 2.8796886690042974E-4 6.544746975009767E-6 4.581322882506837E-5 2.388832645878565E-4 62-63 6.33843037136956 2.945136138754395E-4 6.544746975009767E-6 4.908560231257325E-5 2.536089452816285E-4 64-65 7.054653120393009 3.010583608504493E-4 6.544746975009767E-6 4.908560231257325E-5 2.5851750551288583E-4 66-67 7.7945596725322766 3.0760310782545905E-4 6.544746975009767E-6 4.908560231257325E-5 2.683346259754005E-4 68-69 8.557202675662726 3.0760310782545905E-4 6.544746975009767E-6 4.908560231257325E-5 2.781517464379151E-4 70-71 9.3424152387365 3.0760310782545905E-4 6.544746975009767E-6 4.908560231257325E-5 2.830603066691724E-4 72-73 10.142705262653948 3.1087548131296394E-4 6.544746975009767E-6 5.0721789056325695E-5 2.863326801566773E-4 74-75 10.950441572442216 3.22328788519231E-4 6.544746975009767E-6 5.235797580007814E-5 2.945136138754395E-4 76-77 11.774205967572284 3.321459089817457E-4 6.544746975009767E-6 5.399416254383058E-5 2.977859873629444E-4 78-79 12.609955430600337 3.468715896755177E-4 6.544746975009767E-6 6.217509626259279E-5 3.0760310782545905E-4 80-81 13.458887650222804 3.6650583060054693E-4 6.544746975009767E-6 6.217509626259279E-5 3.125116680567164E-4 82-83 14.31309493590713 3.877762582693287E-4 6.544746975009767E-6 6.217509626259279E-5 3.2560116200673595E-4 84-85 15.17319576624245 4.0741049919435803E-4 6.544746975009767E-6 6.217509626259279E-5 3.3050972223799325E-4 86-87 16.04100958088782 4.1068287268186286E-4 6.544746975009767E-6 6.217509626259279E-5 3.3050972223799325E-4 88 16.696608338681756 4.188638064006251E-4 6.544746975009767E-6 6.217509626259279E-5 3.3050972223799325E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 186450 0.0 86.42652 8 GTGGTAT 189060 0.0 85.228424 6 TGGTATC 188990 0.0 85.04127 7 GTATCAA 189450 0.0 84.89925 9 AGTGGTA 190185 0.0 84.7465 5 AAGCAGT 199460 0.0 81.15799 1 GCAGTGG 200130 0.0 81.09297 3 CAGTGGT 199790 0.0 80.58762 4 AGCAGTG 207765 0.0 78.14877 2 CAACGCA 183175 0.0 44.741066 12-13 ATCAACG 185045 0.0 44.41585 10-11 TCAACGC 182745 0.0 44.181908 12-13 ACGCAGA 186175 0.0 44.036797 14-15 AACGCAG 184760 0.0 43.772568 14-15 AGAGTAC 185400 0.0 43.746128 18-19 GAGTACT 181320 0.0 43.628387 20-21 CGCAGAG 187805 0.0 43.012924 16-17 TATCAAC 189490 0.0 42.460495 10-11 AGTACTT 191205 0.0 42.020954 20-21 GTACTTT 190720 0.0 41.472725 22-23 >>END_MODULE