FastQCFastQC Report
Thu 26 May 2016
SRR1660761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30558859
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16453725.384271709882885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA915930.2997265048410348No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT311470.1019246170153146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2062050.086.641698
GTGGTAT2087550.085.844466
TGGTATC2090800.085.495257
AGTGGTA2098850.085.463725
GTATCAA2089150.085.382839
AAGCAGT2147200.082.028581
GCAGTGG2193250.081.945983
CAGTGGT2189200.081.863584
AGCAGTG2260300.079.423632
CAACGCA2019600.044.893412-13
ATCAACG2033800.044.61807610-11
ACGCAGA2052300.044.26971414-15
TCAACGC2015000.044.2367612-13
AACGCAG2031500.043.91435614-15
AGAGTAC2064250.043.80812518-19
GAGTACT2018050.043.6956420-21
CGCAGAG2069800.043.21983716-17
TATCAAC2084750.042.76348510-11
AGTACTT2110900.042.4906420-21
GTACTTT2098550.041.94970322-23
Produced by FastQC (version 0.11.5)