##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660761_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30558859 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.981606544930226 33.0 31.0 34.0 27.0 34.0 2 31.76438442940556 34.0 31.0 34.0 28.0 34.0 3 32.268091848586366 34.0 31.0 34.0 30.0 34.0 4 35.805267958466644 37.0 35.0 37.0 35.0 37.0 5 35.86051792706004 37.0 35.0 37.0 35.0 37.0 6 35.89894851767862 37.0 35.0 37.0 35.0 37.0 7 35.888998866089864 37.0 35.0 37.0 35.0 37.0 8 35.897575462487 37.0 35.0 37.0 35.0 37.0 9 37.603890348131124 39.0 37.0 39.0 35.0 39.0 10-11 37.53798958920554 39.0 37.0 39.0 35.0 39.0 12-13 37.603444667224 39.0 37.0 39.0 35.0 39.0 14-15 38.924087954331014 40.0 38.0 41.0 35.0 41.0 16-17 38.935560454007785 40.0 38.0 41.0 35.0 41.0 18-19 38.913292476005076 40.0 38.0 41.0 35.0 41.0 20-21 38.84442221812012 40.0 38.0 41.0 35.0 41.0 22-23 38.73649088141674 40.0 38.0 41.0 34.5 41.0 24-25 38.68082717028146 40.0 38.0 41.0 34.0 41.0 26-27 38.63869300552092 40.0 38.0 41.0 34.0 41.0 28-29 38.53524886187668 40.0 38.0 41.0 34.0 41.0 30-31 38.43541013753164 40.0 38.0 41.0 34.0 41.0 32-33 38.329460255698685 40.0 38.0 41.0 34.0 41.0 34-35 38.21771581851273 40.0 38.0 41.0 33.0 41.0 36-37 38.030597510201545 40.0 38.0 41.0 33.0 41.0 38-39 37.60903334119903 40.0 36.0 41.0 33.0 41.0 40-41 37.45549675136758 40.0 36.0 41.0 33.0 41.0 42-43 37.291319286495614 39.5 35.0 41.0 32.0 41.0 44-45 37.168493136474765 39.0 35.0 41.0 32.0 41.0 46-47 37.03074206730035 39.0 35.0 41.0 31.0 41.0 48-49 37.148595093161035 39.0 35.0 41.0 32.0 41.0 50-51 37.050490252270215 39.0 35.0 41.0 31.0 41.0 52-53 36.784778548178124 39.0 35.0 41.0 31.0 41.0 54-55 36.287578227315365 39.0 35.0 41.0 29.0 41.0 56-57 35.99031624511897 39.0 35.0 41.0 28.0 41.0 58-59 35.78723942212633 39.0 35.0 41.0 27.0 41.0 60-61 35.58349870000055 38.5 35.0 41.0 27.0 41.0 62-63 35.29026514046221 38.0 35.0 40.0 26.0 41.0 64-65 35.03853378491651 37.0 34.0 40.0 26.0 41.0 66-67 34.7713967167426 37.0 34.0 40.0 26.0 41.0 68-69 34.485038332092174 36.5 34.0 39.5 26.0 41.0 70-71 34.16160611559482 36.0 34.0 39.0 26.0 41.0 72-73 33.84967908978539 35.0 33.5 39.0 26.0 40.5 74-75 33.496028451193155 35.0 33.0 37.5 25.5 40.0 76-77 31.410315663290962 33.5 30.0 35.5 21.5 38.5 78-79 32.6803895394131 35.0 32.5 36.0 24.5 39.0 80-81 32.674070766189274 35.0 33.0 36.0 25.0 38.5 82-83 32.46666217151629 35.0 33.0 36.0 24.0 37.0 84-85 32.247020593929896 35.0 33.0 35.5 24.0 37.0 86-87 32.05586257327212 35.0 33.0 35.0 24.0 36.5 88-89 31.85165337815787 35.0 33.0 35.0 23.5 36.0 90-91 31.705302233306554 35.0 33.0 35.0 23.0 36.0 92-93 31.587762553569164 35.0 33.0 35.0 22.5 36.0 94-95 31.496128749440548 35.0 32.5 35.0 20.0 35.0 96-97 31.396869644249477 35.0 32.0 35.0 20.0 35.0 98-99 31.284392800791416 35.0 32.0 35.0 20.0 35.0 100 31.099964923428587 35.0 32.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7564556260785302 1101 2 1.2108924211612084 1101 3 0.7762091472924482 1101 4 0.6241202050179382 1101 5 0.46276949650719246 1101 6 0.4056595998928856 1101 7 0.39016460511513174 1101 8 0.4134313866328867 1101 9 0.512350642360623 1101 10-11 0.49485371876362194 1101 12-13 0.4535631714163486 1101 14-15 0.6108987104470813 1101 16-17 0.5950687335712885 1101 18-19 0.5437516238212581 1101 20-21 0.5886105488868978 1101 22-23 0.5470021916926342 1101 24-25 0.5933870957403542 1101 26-27 0.5812794430847603 1101 28-29 0.6060227181742093 1101 30-31 0.5848453180235964 1101 32-33 0.5666258935102775 1101 34-35 0.5979051632249011 1101 36-37 0.5906407836370278 1101 38-39 0.650718654672005 1101 40-41 0.6183338701040739 1101 42-43 0.5653725977686577 1101 44-45 0.533969658790518 1101 46-47 0.5153907954537118 1101 48-49 0.5021970105479951 1101 50-51 0.4912250545878507 1101 52-53 0.5235838650542419 1101 54-55 0.5552172044382147 1101 56-57 0.6005544889693937 1101 58-59 0.5546179858541294 1101 60-61 0.5107665461567663 1101 62-63 0.5361595545781057 1101 64-65 0.5106776066388861 1101 66-67 0.46923824121604696 1101 68-69 0.47640901441596384 1101 70-71 0.45612538906889455 1101 72-73 0.44572196717087564 1101 74-75 0.4371363310307501 1101 76-77 0.35778420221210894 1101 78-79 0.47226000002055457 1101 80-81 0.44890677478297647 1101 82-83 0.41062656615662974 1101 84-85 0.4087217036124713 1101 86-87 0.38509282575775217 1101 88-89 0.42656823971434576 1101 90-91 0.44624334478987393 1101 92-93 0.38293335505997206 1101 94-95 0.36154355825879847 1101 96-97 0.3476434345057271 1101 98-99 0.3542438609020131 1101 100 0.32180877466248603 1102 1 1.652586912925603 1102 2 1.1487589550211688 1102 3 0.6999474767039899 1102 4 0.5695491550155722 1102 5 0.4129676046015902 1102 6 0.38931745714052113 1102 7 0.35696286734660987 1102 8 0.37994985895476674 1102 9 0.4780604324386246 1102 10-11 0.45823915718916197 1102 12-13 0.379570390294262 1102 14-15 0.5489463236527996 1102 16-17 0.5356041066458275 1102 18-19 0.5128608564307555 1102 20-21 0.49279884800228757 1102 22-23 0.49397784704017766 1102 24-25 0.5576425193561576 1102 26-27 0.5541242147108605 1102 28-29 0.5503782681294567 1102 30-31 0.5282758740073916 1102 32-33 0.5226670405862137 1102 34-35 0.512105167525732 1102 36-37 0.544660440487732 1102 38-39 0.6236198364502243 1102 40-41 0.5526232973710634 1102 42-43 0.5657268592583691 1102 44-45 0.5772478398673329 1102 46-47 0.5793576224046078 1102 48-49 0.573583021594402 1102 50-51 0.5536476613414578 1102 52-53 0.4961233422317761 1102 54-55 0.5539071606592572 1102 56-57 0.6411206050898173 1102 58-59 0.5859563811568549 1102 60-61 0.5675476616583026 1102 62-63 0.6067753013844737 1102 64-65 0.562123911617185 1102 66-67 0.5251473226311347 1102 68-69 0.5117528035219152 1102 70-71 0.5039965663847354 1102 72-73 0.44228408125584195 1102 74-75 0.4644995275118404 1102 76-77 0.3782066020182917 1102 78-79 0.5343004406491545 1102 80-81 0.5342023702426602 1102 82-83 0.5077716505394889 1102 84-85 0.47790687502300955 1102 86-87 0.46555884297623606 1102 88-89 0.48697429087920696 1102 90-91 0.5239896920596685 1102 92-93 0.47066941909336535 1102 94-95 0.4168038179761062 1102 96-97 0.4331713715001442 1102 98-99 0.45030792172988043 1102 100 0.38184108850591514 1103 1 1.6515098691917665 1103 2 1.1081741973074593 1103 3 0.6651074813844744 1103 4 0.551032732420687 1103 5 0.4139558477618479 1103 6 0.3861329528003381 1103 7 0.3478842590349487 1103 8 0.3794528080131414 1103 9 0.4416057185000284 1103 10-11 0.4080182303956974 1103 12-13 0.356976614109243 1103 14-15 0.5447988979169054 1103 16-17 0.5269295761482553 1103 18-19 0.46125708589433145 1103 20-21 0.45377785880237553 1103 22-23 0.47632196692991613 1103 24-25 0.527136494240878 1103 26-27 0.4980023126121367 1103 28-29 0.49632655684485627 1103 30-31 0.489631230466685 1103 32-33 0.5049206581446839 1103 34-35 0.4913474768070074 1103 36-37 0.4543276666451277 1103 38-39 0.5323523397263443 1103 40-41 0.5206316878158432 1103 42-43 0.4493193963754365 1103 44-45 0.5217432503665194 1103 46-47 0.5458727730980115 1103 48-49 0.5232772569844499 1103 50-51 0.46691172512645096 1103 52-53 0.47863438311542694 1103 54-55 0.5048752597501434 1103 56-57 0.5755868599982463 1103 58-59 0.5588453125969082 1103 60-61 0.5286326809198272 1103 62-63 0.5491025797189195 1103 64-65 0.5214032113945635 1103 66-67 0.4804800742274793 1103 68-69 0.515716914231902 1103 70-71 0.49238014048701473 1103 72-73 0.42700760717564634 1103 74-75 0.4384470632968913 1103 76-77 0.311694876274597 1103 78-79 0.4118767194201851 1103 80-81 0.4446736333849586 1103 82-83 0.47242803822171453 1103 84-85 0.4570948975848097 1103 86-87 0.43886659256960314 1103 88-89 0.476991667319016 1103 90-91 0.49486607030882723 1103 92-93 0.45438871572313744 1103 94-95 0.38915660806140906 1103 96-97 0.38635332222951746 1103 98-99 0.4215685705029095 1103 100 0.2967031079473301 1104 1 1.5963566018130244 1104 2 1.0900662783737154 1104 3 0.6250975615292163 1104 4 0.519226636459905 1104 5 0.3786847844495824 1104 6 0.3319502615489256 1104 7 0.29295650382811544 1104 8 0.29965894023430195 1104 9 0.3899668548767323 1104 10-11 0.3646044233761643 1104 12-13 0.3277119858394002 1104 14-15 0.5000842825262097 1104 16-17 0.4671329385857561 1104 18-19 0.4205110032041688 1104 20-21 0.4058411362158054 1104 22-23 0.3971703639989599 1104 24-25 0.4424045050172154 1104 26-27 0.4144104328776237 1104 28-29 0.4280007321957555 1104 30-31 0.42538039675100947 1104 32-33 0.42032157128923586 1104 34-35 0.37758827143800033 1104 36-37 0.4300545567611067 1104 38-39 0.48130605021177786 1104 40-41 0.45912365316643644 1104 42-43 0.4301755072109259 1104 44-45 0.4167240460048802 1104 46-47 0.4751159514954253 1104 48-49 0.46324252805128197 1104 50-51 0.43204151242651534 1104 52-53 0.42590156144778035 1104 54-55 0.47888098410665947 1104 56-57 0.5266031702405414 1104 58-59 0.46168607766828273 1104 60-61 0.44814154708392806 1104 62-63 0.5356199169227267 1104 64-65 0.4766100113189893 1104 66-67 0.4753626489141354 1104 68-69 0.4570434588061332 1104 70-71 0.48356003061773123 1104 72-73 0.4382931941477253 1104 74-75 0.3865499106645345 1104 76-77 0.36595606050348906 1104 78-79 0.41047986038357465 1104 80-81 0.3947124466479295 1104 82-83 0.3928155786906018 1104 84-85 0.41868781770679675 1104 86-87 0.4095680955331318 1104 88-89 0.42112316840300323 1104 90-91 0.4576891773765759 1104 92-93 0.4250901459321277 1104 94-95 0.42029853761235714 1104 96-97 0.3962703510695036 1104 98-99 0.4329504820398853 1104 100 0.3968336572258586 1105 1 1.582250725398854 1105 2 1.0536919464852978 1105 3 0.6103957230302797 1105 4 0.5001825312705321 1105 5 0.35571974554741814 1105 6 0.2895282672608772 1105 7 0.22678453311655744 1105 8 0.2890297742953081 1105 9 0.3533213627536753 1105 10-11 0.31677224751949495 1105 12-13 0.30065061974977425 1105 14-15 0.39688910492306917 1105 16-17 0.35383574706370524 1105 18-19 0.34668906038761094 1105 20-21 0.3423436739432262 1105 22-23 0.304100197586159 1105 24-25 0.363605330297716 1105 26-27 0.3478009042134218 1105 28-29 0.3379341514344887 1105 30-31 0.3682462093317298 1105 32-33 0.3905026949386041 1105 34-35 0.38736146972790664 1105 36-37 0.42400146735192834 1105 38-39 0.4264125230092901 1105 40-41 0.4320605052511368 1105 42-43 0.3987669366165463 1105 44-45 0.4295342073289632 1105 46-47 0.47794810819194566 1105 48-49 0.44398477024390814 1105 50-51 0.4209838488381408 1105 52-53 0.390690529013753 1105 54-55 0.4331438173824651 1105 56-57 0.42206374700280236 1105 58-59 0.4440004115694691 1105 60-61 0.431032932616894 1105 62-63 0.4386059825907722 1105 64-65 0.4137357854636363 1105 66-67 0.43141308308033643 1105 68-69 0.4861149433690599 1105 70-71 0.42960220479047706 1105 72-73 0.3903395144266639 1105 74-75 0.3210302155424145 1105 76-77 0.310333211161975 1105 78-79 0.43484070575749456 1105 80-81 0.41093914403714393 1105 82-83 0.4043339215004593 1105 84-85 0.42649991216734406 1105 86-87 0.4202850873026307 1105 88-89 0.4355218842138875 1105 90-91 0.452443277077176 1105 92-93 0.40899917983514555 1105 94-95 0.4120993147723553 1105 96-97 0.3855051022292102 1105 98-99 0.38176243372337737 1105 100 0.3710880598334221 1106 1 1.4278636094065895 1106 2 0.8794217576230032 1106 3 0.4770431430490234 1106 4 0.42085533839829736 1106 5 0.24693247224518444 1106 6 0.22262070954887037 1106 7 0.17652744034845824 1106 8 0.2026590507310786 1106 9 0.24519851326667208 1106 10-11 0.24208613754108654 1106 12-13 0.20844751591721433 1106 14-15 0.3158151126510589 1106 16-17 0.24554747290150658 1106 18-19 0.19616007470646935 1106 20-21 0.21622159028297716 1106 22-23 0.20650900521503246 1106 24-25 0.25552979645357254 1106 26-27 0.23381629194533815 1106 28-29 0.2753866270445968 1106 30-31 0.2902278697229619 1106 32-33 0.2552791815334743 1106 34-35 0.2613342140383068 1106 36-37 0.27590875031144435 1106 38-39 0.3417376200112088 1106 40-41 0.32456846754983104 1106 42-43 0.29738359988024854 1106 44-45 0.2778175806091099 1106 46-47 0.2976959968254391 1106 48-49 0.3007934166902757 1106 50-51 0.25295897997543904 1106 52-53 0.20124790281507643 1106 54-55 0.29904949531918845 1106 56-57 0.2749812437998074 1106 58-59 0.2532960637205903 1106 60-61 0.23155231263881149 1106 62-63 0.25324438345102607 1106 64-65 0.2486366654641472 1106 66-67 0.23736346006495523 1106 68-69 0.21642527900934994 1106 70-71 0.2510128656500754 1106 72-73 0.26803114245068116 1106 74-75 0.22586567217912545 1106 76-77 0.15203645661031828 1106 78-79 0.2880992605378836 1106 80-81 0.28977267990057953 1106 82-83 0.2838662488373487 1106 84-85 0.2817889577193853 1106 86-87 0.24849990486658413 1106 88-89 0.2935652331806189 1106 90-91 0.31402123813189675 1106 92-93 0.2715449293149881 1106 94-95 0.2666535760374167 1106 96-97 0.31155025624676824 1106 98-99 0.3030616812748832 1106 100 0.2584127896409356 1107 1 1.5638127334317709 1107 2 1.022976143830757 1107 3 0.5757434653184603 1107 4 0.488551727478999 1107 5 0.33424190070979876 1107 6 0.3217234858058191 1107 7 0.2698438068019158 1107 8 0.2858062165966544 1107 9 0.35291638639629497 1107 10-11 0.32714980961431905 1107 12-13 0.3026682157339664 1107 14-15 0.41836588616033765 1107 16-17 0.3881192851228761 1107 18-19 0.35008641318994904 1107 20-21 0.357604665843688 1107 22-23 0.37648662103110553 1107 24-25 0.3954736923875686 1107 26-27 0.3697862059682606 1107 28-29 0.3459354838546389 1107 30-31 0.38754498288501793 1107 32-33 0.4013046683719921 1107 34-35 0.4048366334080882 1107 36-37 0.4678635426533333 1107 38-39 0.4643433734236737 1107 40-41 0.4763563268784665 1107 42-43 0.44286384926277833 1107 44-45 0.4344955645224289 1107 46-47 0.5142948013739286 1107 48-49 0.465607901766937 1107 50-51 0.4284383211753493 1107 52-53 0.3829715367435469 1107 54-55 0.4163561722656155 1107 56-57 0.394270626626259 1107 58-59 0.41044979405758397 1107 60-61 0.33310791059773237 1107 62-63 0.38191350430241755 1107 64-65 0.3743267651708706 1107 66-67 0.3606663918102555 1107 68-69 0.3581301389728324 1107 70-71 0.38471397179932154 1107 72-73 0.3667707081925684 1107 74-75 0.36351045341010035 1107 76-77 0.26550961925389416 1107 78-79 0.38865281094792437 1107 80-81 0.33100397173015494 1107 82-83 0.314879278533688 1107 84-85 0.33114642042049525 1107 86-87 0.3152263574128398 1107 88-89 0.36718132915203583 1107 90-91 0.41229824029013784 1107 92-93 0.3965174239207023 1107 94-95 0.3760886946055457 1107 96-97 0.3763313089799567 1107 98-99 0.3781489629423902 1107 100 0.3489245705018966 1108 1 1.389291655507396 1108 2 0.8787465745031575 1108 3 0.4325478996010119 1108 4 0.3754116322293015 1108 5 0.20081699662787145 1108 6 0.14384356792815822 1108 7 0.0955612511225894 1108 8 0.09979130552746796 1108 9 0.13381265738243542 1108 10-11 0.10727855313005819 1108 12-13 0.10308890428880346 1108 14-15 0.14718038009316814 1108 16-17 0.09955408005243527 1108 18-19 0.08835778763801017 1108 20-21 0.09199655924583539 1108 22-23 0.06729853443112432 1108 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sequence quality scores pass #Quality Count 6 58.0 7 828.0 8 1101.0 9 425.0 10 217.0 11 193.0 12 317.0 13 1315.0 14 5628.0 15 14663.0 16 25288.0 17 34619.0 18 44575.0 19 58013.0 20 79531.0 21 113247.0 22 170803.0 23 268465.0 24 421183.0 25 540470.0 26 543686.0 27 478168.0 28 451802.0 29 488472.0 30 570769.0 31 691467.0 32 849963.0 33 1073326.0 34 1446873.0 35 2137951.0 36 3616214.0 37 6592574.0 38 7821260.0 39 2011395.0 40 4000.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.651611306922977 33.87829064642638 27.550319671476775 19.919778375173863 2 21.938011494473663 32.94719544339008 26.02720212819464 19.087590933941613 3 26.346481064623518 26.16473016875401 25.936842079084172 21.5519466875383 4 20.873560756964128 26.85417999408944 25.72564963894758 26.546609609998857 5 21.23081430297902 32.22735325874091 25.699971619411066 20.84186081886901 6 27.30219737589025 26.09666152784042 25.688030433335225 20.91311066293411 7 20.93826539793256 26.244487727764966 31.853954363937476 20.963292510364997 8 26.97495675476627 26.33638252004108 25.744766844861584 20.943893880331068 9 26.865499133982716 25.96969670889872 26.086729874305842 21.07807428281272 10-11 20.626280582007332 29.54032740554875 29.050953767612853 20.782438244831063 12-13 20.646469490238495 26.404727676514362 28.96701411528487 23.981788717962278 14-15 20.686652600478308 32.6265028416146 25.766312151903314 20.920532406003773 16-17 23.9056242250406 26.28093215129531 25.781620315077863 24.03182330858623 18-19 20.807021051042067 29.40795354379167 25.730962299015015 24.05406310615125 20-21 23.95083194674588 29.400799328265652 25.706127874097035 20.942240850891434 22-23 23.936000031938363 26.353223790194523 28.823129489226023 20.88764668864109 24-25 20.949080938919153 29.37772144085567 25.555681852040006 24.117515768185168 26-27 20.971071183698296 26.27607805953579 31.78943075546574 20.96342000130018 28-29 20.968184708728817 26.35850867337684 31.77635984380176 20.89694677409258 30-31 20.9959622510775 26.320352798512538 31.73730406622839 20.946380884181572 32-33 21.00669236374303 26.34781619300642 31.69578255523218 20.949708888018364 34-35 21.04612773664095 26.370658996135948 31.652968456708415 20.930244810514687 36-37 21.09269430159988 26.368833874616648 31.568771319923016 20.96970050386045 38-39 21.124910128352635 26.38090316133858 31.52788688870877 20.966299821600014 40-41 21.133408731244344 26.48793673513431 31.506972015933933 20.87168251768741 42-43 21.170146798394914 26.51714437536894 31.418962980500037 20.89374584573611 44-45 21.235585138698255 26.56132158644376 31.31395001094367 20.889143263914317 46-47 21.268763470442288 26.671131704258936 31.21282336281505 20.84728146248373 48-49 21.33307406987325 26.686245486587275 31.086114825133627 20.894565618405856 50-51 21.33882553237386 26.770888709733708 30.967972838383346 20.922312919509086 52-53 21.432834573468632 26.939746063168535 30.702721228136532 20.9246981352263 54-55 21.793232901188354 27.18110076260581 29.921765849664162 21.103900486541676 56-57 22.21751632715611 27.657162151221726 28.745134184493676 21.38018733712849 58-59 22.314644618019177 28.1682580277642 28.04392907311862 21.473168281098005 60-61 22.34856727161728 28.44389003741331 27.613310291179697 21.59423239978971 62-63 22.306824937614078 28.634046306335385 27.40209093770194 21.65703781834859 64-65 22.24403278330709 28.745883556790186 27.332314097604147 21.677769562298575 66-67 22.203802350250587 28.565213851441207 27.444694788902595 21.78628900940561 68-69 22.11344710949243 28.392516557305612 27.64655425967505 21.847482073526912 70-71 22.011556366147413 28.268936210428304 27.8375686354356 21.88193878798868 72-73 21.952894668173112 28.13460964404856 27.909220591826283 22.003275095952045 74-75 21.93969414391656 28.116153095786327 27.845902981897687 22.098249778399424 76-77 21.96493372733022 28.16845230559829 27.706709081594198 22.159904885477292 78-79 22.028279330321034 28.246796549224328 27.3662821154004 22.358642005054246 80-81 22.054336871362874 28.356088125134725 27.063422501896667 22.52615250160574 82-83 22.02457427285465 28.478075209304404 26.830228325396405 22.667122192444545 84-85 22.022647423802898 28.462924608926134 26.67119050317651 22.84323746409446 86-87 22.027547059405737 28.419649277749652 26.537955357252862 23.01484830559174 88-89 22.065671236384627 28.35903232100847 26.436321137737462 23.138975304869444 90-91 22.03024272307425 28.231846346760726 26.37808659565289 23.35982433451214 92-93 22.030517147507815 28.115887064320493 26.411109388657284 23.442486399514408 94-95 22.038332872380515 28.06000200176186 26.407925065843056 23.493740060014566 96-97 22.039067973350395 27.926066211929694 26.450115508146833 23.584750306573074 98-99 21.99973789545563 27.821359962596514 26.47413475024037 23.704767391707485 100 21.976119961252973 27.80917657778874 26.457084203294073 23.75761925766421 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 54538.0 1 34439.5 2 11323.5 3 7913.0 4 7389.0 5 7364.0 6 7529.5 7 7814.5 8 8150.0 9 8397.5 10 9136.0 11 12081.0 12 112554.5 13 137517.5 14 62106.5 15 64044.0 16 73742.5 17 83562.5 18 92875.5 19 102875.5 20 114609.0 21 132068.0 22 155258.0 23 179389.5 24 203164.0 25 231234.0 26 261205.0 27 295703.5 28 343908.0 29 393791.5 30 434191.0 31 477806.0 32 527147.5 33 567827.5 34 604256.0 35 654072.0 36 708057.5 37 750860.5 38 801194.0 39 845262.5 40 872983.0 41 915929.5 42 971261.5 43 993232.0 44 992897.5 45 1012446.0 46 1035817.0 47 1048812.5 48 1055767.0 49 1059046.0 50 1061119.0 51 1067327.0 52 1061570.0 53 1025135.0 54 970291.5 55 909905.5 56 842454.0 57 764565.0 58 676822.5 59 579626.0 60 477367.5 61 385916.0 62 305329.0 63 231806.5 64 173189.5 65 127254.5 66 91913.0 67 67783.5 68 50853.5 69 38802.5 70 29694.5 71 23120.5 72 18872.5 73 15132.5 74 12200.0 75 10668.5 76 8368.5 77 5057.5 78 2999.5 79 2044.0 80 1391.5 81 965.0 82 634.0 83 431.5 84 293.0 85 212.0 86 138.0 87 83.5 88 58.5 89 50.0 90 39.0 91 29.0 92 22.0 93 13.0 94 10.0 95 7.0 96 5.0 97 3.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10692807607770957 2 0.0 3 0.0 4 0.0 5 0.009041567945975994 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.07217890563257E-5 20-21 0.0108888227796725 22-23 0.0 24-25 8.29546679082488E-4 26-27 0.004877472683126029 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.2723734875048836E-6 38-39 0.0 40-41 0.007557546569392528 42-43 0.03904268807942077 44-45 0.015286892746879064 46-47 0.004730215876188309 48-49 0.0016263696232899272 50-51 1.6361867437524418E-6 52-53 0.0014153015333458622 54-55 5.726653603133546E-5 56-57 8.540894802387747E-4 58-59 2.617898790003907E-5 60-61 2.732431862066578E-4 62-63 2.66698439231648E-4 64-65 4.5813228825068374E-5 66-67 2.454280115628663E-4 68-69 4.679494087131984E-4 70-71 3.8614007152557625E-4 72-73 0.0011944163229392825 74-75 0.001636186743752442 76-77 0.0024526439288849104 78-79 0.004520783972987997 80-81 0.004142824835181183 82-83 0.007289211943417129 84-85 0.002374106965184793 86-87 0.0028158773859979524 88-89 0.005633390958739657 90-91 0.005530311193883254 92-93 0.005491042712033195 94-95 0.0025475427600225517 96-97 0.005585941543170836 98-99 0.007688441508892724 100 0.2993829056248468 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0558859E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.81834643346474 #Duplication Level Percentage of deduplicated Percentage of total 1 56.44680050746813 19.089374546222306 2 20.297510040002216 13.728564525390475 3 9.381104753835578 9.517603514815034 4 4.544666029481938 6.147723608376754 5 2.4253864965171537 4.10112803871322 6 1.4937512367782144 3.030971808646924 7 0.9681109829777144 2.29179388258677 8 0.7256326050865167 1.9631755857786661 9 0.5372391287718911 1.6351685076978526 >10 2.810429524481752 17.166421522773472 >50 0.19889950145387664 4.633511070389577 >100 0.16895578327293187 10.164706585745328 >500 8.500594781568081E-4 0.18295421047164484 >1k 6.058193563898713E-4 0.4429136894727531 >5k 9.588506238182122E-6 0.02096064639998103 >10k+ 4.794253118798872E-5 5.883028256519298 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1645372 5.384271709882885 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 91593 0.2997265048410348 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 31147 0.1019246170153146 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.008036949285311994 0.0 0.0 0.0 3.599610836255372E-5 2 0.008059855899724529 2.2906614412534187E-5 0.0 0.0 3.599610836255372E-5 3 0.008072945393674548 2.2906614412534187E-5 0.0 3.2723734875048836E-6 3.599610836255372E-5 4 0.008092579634599577 2.617898790003907E-5 0.0 3.2723734875048836E-6 3.599610836255372E-5 5 0.008092579634599577 2.617898790003907E-5 0.0 3.2723734875048836E-6 3.92684818500586E-5 6 0.008092579634599577 2.617898790003907E-5 0.0 6.544746975009767E-6 4.5813228825068374E-5 7 0.008092579634599577 3.272373487504884E-5 0.0 6.544746975009767E-6 4.5813228825068374E-5 8 0.008102396755062092 3.599610836255372E-5 0.0 6.544746975009767E-6 5.235797580007814E-5 9 0.008112213875524606 4.254085533756349E-5 0.0 9.81712046251465E-6 6.871984323760255E-5 10-11 0.008136756676680894 4.417704208131593E-5 0.0 1.3089493950019534E-5 6.871984323760255E-5 12-13 0.008159663291093428 5.235797580007814E-5 0.0 1.96342409250293E-5 6.871984323760255E-5 14-15 0.008205476519918497 5.7266536031335465E-5 0.0 2.1270427668781744E-5 6.871984323760255E-5 16-17 0.008252925935487317 6.217509626259279E-5 3.2723734875048836E-6 3.272373487504884E-5 9.162645765013675E-5 18-19 0.0083085562847749 7.85369637001172E-5 3.2723734875048836E-6 3.599610836255372E-5 9.489883113764162E-5 20-21 0.008354369513599968 9.162645765013675E-5 3.2723734875048836E-6 3.599610836255372E-5 9.81712046251465E-5 22-23 0.008585071844469062 9.81712046251465E-5 3.2723734875048836E-6 4.5813228825068374E-5 1.0307976485640383E-4 24-25 0.009774579607177088 1.0307976485640383E-4 3.2723734875048836E-6 4.5813228825068374E-5 1.0798832508766116E-4 26-27 0.01453424684475294 1.1126069857516605E-4 3.2723734875048836E-6 4.5813228825068374E-5 1.1780544555017582E-4 28-29 0.02812277775161697 1.2271400578143314E-4 3.2723734875048836E-6 4.5813228825068374E-5 1.1780544555017582E-4 30-31 0.05817461967411807 1.3416731298770022E-4 3.2723734875048836E-6 4.5813228825068374E-5 1.3580349973145266E-4 32-33 0.11461488139985855 1.374396864752051E-4 3.2723734875048836E-6 4.5813228825068374E-5 1.5052918042522464E-4 34-35 0.20716414837347166 1.5380155391272953E-4 3.2723734875048836E-6 4.5813228825068374E-5 1.603463008877393E-4 36-37 0.34258805278037374 1.6361867437524418E-4 3.2723734875048836E-6 4.5813228825068374E-5 1.8488910204402594E-4 38-39 0.5256446256713969 1.7834435506901617E-4 3.2723734875048836E-6 4.5813228825068374E-5 1.96342409250293E-4 40-41 0.7580698611816625 1.799805418127686E-4 3.2723734875048836E-6 4.5813228825068374E-5 1.96342409250293E-4 42-43 1.0410156347787725 1.8161672855652105E-4 3.2723734875048836E-6 4.5813228825068374E-5 2.0288715622530278E-4 44-45 1.3731991760556244 1.8979766227528324E-4 3.2723734875048836E-6 4.5813228825068374E-5 2.0452334296905522E-4 46-47 1.7583019051856614 2.11068089944065E-4 3.2723734875048836E-6 4.5813228825068374E-5 2.0943190320031255E-4 48-49 2.1966723299453035 2.2906614412534186E-4 3.2723734875048836E-6 4.5813228825068374E-5 2.1434046343156988E-4 50-51 2.682146934870834 2.5197275853787606E-4 3.2723734875048836E-6 4.5813228825068374E-5 2.2088521040657965E-4 52-53 3.2083887687037005 2.6015369225663824E-4 3.2723734875048836E-6 4.5813228825068374E-5 2.2579377063783698E-4 54-55 3.7738598159047756 2.683346259754005E-4 3.2723734875048836E-6 4.908560231257325E-5 2.2742995738158942E-4 56-57 4.381724461636477 2.8142411992541997E-4 3.2723734875048836E-6 5.235797580007814E-5 2.3561089110035163E-4 58-59 5.024531511467755 2.830603066691724E-4 3.2723734875048836E-6 5.235797580007814E-5 2.3561089110035163E-4 60-61 5.702038809760534 2.8960505364418216E-4 3.2723734875048836E-6 5.235797580007814E-5 2.454280115628663E-4 62-63 6.4028748586457365 3.125116680567164E-4 3.2723734875048836E-6 5.399416254383058E-5 2.536089452816285E-4 64-65 7.126282103661004 3.2723734875048837E-4 3.2723734875048836E-6 5.563034928758302E-5 2.617898790003907E-4 66-67 7.874014536995638 3.3050972223799325E-4 3.2723734875048836E-6 5.563034928758302E-5 2.732431862066578E-4 68-69 8.644797569176259 3.435992161880128E-4 3.2723734875048836E-6 5.563034928758302E-5 3.010583608504493E-4 70-71 9.439794528977668 3.730505775755567E-4 3.2723734875048836E-6 5.7266536031335465E-5 3.010583608504493E-4 72-73 10.250714858169278 4.0086575221934826E-4 3.2723734875048836E-6 5.890272277508791E-5 3.0433073433795417E-4 74-75 11.070581856475727 4.2540855337563487E-4 3.2723734875048836E-6 6.544746975009768E-5 3.125116680567164E-4 76-77 11.906889259183401 4.4667898104441665E-4 3.2723734875048836E-6 6.544746975009768E-5 3.141478548004688E-4 78-79 12.754975570259347 4.4831516778816907E-4 3.2723734875048836E-6 7.526459021261232E-5 3.141478548004688E-4 80-81 13.61680421379607 4.614046617381886E-4 3.2723734875048836E-6 7.526459021261232E-5 3.1578404154422124E-4 82-83 14.483443246359428 4.92492209869485E-4 3.2723734875048836E-6 7.690077695636476E-5 3.37054469213003E-4 84-85 15.356787372198681 5.03945517075752E-4 3.2723734875048836E-6 8.017315044386965E-5 3.386906559567555E-4 86-87 16.238464597123865 5.186711977695241E-4 3.2723734875048836E-6 8.344552393137453E-5 3.419630294442603E-4 88 16.905084708823715 5.203073845132765E-4 3.2723734875048836E-6 8.508171067512698E-5 3.435992161880128E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 206205 0.0 86.64169 8 GTGGTAT 208755 0.0 85.84446 6 TGGTATC 209080 0.0 85.49525 7 AGTGGTA 209885 0.0 85.46372 5 GTATCAA 208915 0.0 85.38283 9 AAGCAGT 214720 0.0 82.02858 1 GCAGTGG 219325 0.0 81.94598 3 CAGTGGT 218920 0.0 81.86358 4 AGCAGTG 226030 0.0 79.42363 2 CAACGCA 201960 0.0 44.8934 12-13 ATCAACG 203380 0.0 44.618076 10-11 ACGCAGA 205230 0.0 44.269714 14-15 TCAACGC 201500 0.0 44.23676 12-13 AACGCAG 203150 0.0 43.914356 14-15 AGAGTAC 206425 0.0 43.808125 18-19 GAGTACT 201805 0.0 43.69564 20-21 CGCAGAG 206980 0.0 43.219837 16-17 TATCAAC 208475 0.0 42.763485 10-11 AGTACTT 211090 0.0 42.49064 20-21 GTACTTT 209855 0.0 41.949703 22-23 >>END_MODULE