FastQCFastQC Report
Thu 26 May 2016
SRR1660760_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660760_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35618407
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23250086.527546276845003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2221470.623685949795565No Hit
AAGCAGTGGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA818610.22982779662212294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3018400.087.281148
GTGGTAT3041950.086.7504656
AGTGGTA3050800.086.5302055
TGGTATC3051300.086.172267
GTATCAA3073750.085.613179
AAGCAGT3109300.085.259131
GCAGTGG3131500.084.752643
CAGTGGT3183950.082.737514
AGCAGTG3218400.082.5586242
ATCAACG3024900.044.5607910-11
CAACGCA3019000.044.49300812-13
AGAGTAC2870750.044.30075518-19
ACGCAGA3060050.043.84500514-15
TCAACGC3008800.043.8211212-13
GAGTACT2828300.043.7425720-21
AACGCAG3042250.043.32615314-15
TATCAAC3074050.042.72753510-11
CGCAGAG3086500.042.5821516-17
AGTACTT2966100.042.38817220-21
GTACTTT2932150.042.18029422-23
Produced by FastQC (version 0.11.5)