##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660760_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35618407 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775304409318473 33.0 31.0 34.0 30.0 34.0 2 31.92971423455294 34.0 31.0 34.0 30.0 34.0 3 31.97095936940695 34.0 31.0 34.0 30.0 34.0 4 35.39470035816032 37.0 35.0 37.0 33.0 37.0 5 35.390660312236875 37.0 35.0 37.0 33.0 37.0 6 35.39783859508372 37.0 35.0 37.0 33.0 37.0 7 35.358978968374416 37.0 35.0 37.0 33.0 37.0 8 35.37808029988539 37.0 35.0 37.0 33.0 37.0 9 37.00813632681552 39.0 37.0 39.0 33.0 39.0 10-11 36.92688954337571 39.0 37.0 39.0 33.0 39.0 12-13 36.98268551145479 39.0 37.0 39.0 33.0 39.0 14-15 38.29579262205634 40.0 38.0 41.0 33.0 41.0 16-17 38.2492151038647 40.0 38.0 41.0 33.0 41.0 18-19 38.14293280999344 40.0 38.0 41.0 33.0 41.0 20-21 38.05818946366692 40.0 38.0 41.0 33.0 41.0 22-23 37.873546239729365 40.0 37.5 41.0 32.5 41.0 24-25 37.81154193672951 40.0 37.5 41.0 32.0 41.0 26-27 37.78569939975137 40.0 37.5 41.0 32.0 41.0 28-29 37.67475910699768 40.0 37.0 41.0 32.0 41.0 30-31 37.51701102185733 40.0 37.0 41.0 31.5 41.0 32-33 37.344212291695136 40.0 37.0 41.0 31.0 41.0 34-35 37.159739120281266 40.0 37.0 41.0 30.5 41.0 36-37 36.89072404332962 39.5 36.0 41.0 30.0 41.0 38-39 36.336643803300916 39.0 35.0 41.0 30.0 41.0 40-41 36.279638446492 39.0 35.0 41.0 30.0 41.0 42-43 36.25936004100352 39.0 35.0 41.0 29.0 41.0 44-45 36.172027457600784 39.0 35.0 41.0 29.0 41.0 46-47 36.24950844938124 39.0 35.0 41.0 29.0 41.0 48-49 36.154287893897106 39.0 35.0 41.0 28.0 41.0 50-51 35.02014487902281 38.0 34.0 40.0 25.5 40.5 52-53 35.0101942796038 38.5 34.5 39.5 24.5 40.5 54-55 35.224147376944735 39.0 35.0 41.0 22.5 41.0 56-57 34.88270208153891 39.0 34.5 41.0 20.0 41.0 58-59 34.5681659626159 39.0 34.0 41.0 18.0 41.0 60-61 34.31149019382029 38.5 34.0 41.0 17.5 41.0 62-63 34.02794971992992 38.0 33.5 40.5 16.0 41.0 64-65 33.72738107013039 37.0 33.0 40.0 15.0 41.0 66-67 33.421399193961705 37.0 33.0 40.0 13.5 41.0 68-69 33.10469832353816 36.0 32.5 40.0 13.0 41.0 70-71 32.77060982822729 36.0 32.0 39.0 13.0 41.0 72-73 32.421027504121675 35.0 32.0 39.0 12.0 41.0 74-75 32.05908053383746 35.0 31.5 38.5 11.5 40.0 76-77 31.67483200469914 35.0 31.0 37.0 10.0 39.5 78-79 31.312339852818234 35.0 31.0 37.0 10.0 39.0 80-81 30.971633332731585 35.0 30.5 36.0 10.0 39.0 82-83 30.647621663708883 35.0 30.0 36.0 10.0 37.5 84-85 30.36473248228086 35.0 30.0 35.5 10.0 37.0 86-87 30.10073175086129 35.0 30.0 35.0 10.0 37.0 88-89 29.902104591595013 35.0 29.5 35.0 9.0 36.0 90-91 29.72595513606209 34.5 29.0 35.0 8.0 36.0 92-93 29.563105629625717 34.0 29.0 35.0 8.0 36.0 94-95 29.40621134460056 34.0 29.0 35.0 8.0 35.5 96-97 29.292112390090885 34.0 29.0 35.0 8.0 35.0 98-99 29.186804620431225 34.0 29.0 35.0 8.0 35.0 100 29.06358686956438 34.0 28.0 35.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6881357227915466 1101 2 0.649195838435265 1101 3 0.647703300012477 1101 4 0.5673308375506707 1101 5 0.5785655126209406 1101 6 0.6128137247193877 1101 7 0.5380279897498355 1101 8 0.5928649772706223 1101 9 0.7407219389342572 1101 10-11 0.7028815713659853 1101 12-13 0.6758918628969681 1101 14-15 0.8437582040257396 1101 16-17 0.8609376550234629 1101 18-19 0.8366088681496748 1101 20-21 0.7776452293288472 1101 22-23 0.732822317134179 1101 24-25 0.7220671296733983 1101 26-27 0.7325771897264843 1101 28-29 0.6894158002801021 1101 30-31 0.7571322946857819 1101 32-33 0.7495899366455774 1101 34-35 0.7353783990229132 1101 36-37 0.7480378362030038 1101 38-39 0.790394930738934 1101 40-41 0.7661376327161165 1101 42-43 0.6938884472598232 1101 44-45 0.6412481154259453 1101 46-47 0.7526877075471532 1101 48-49 0.7728321600036381 1101 50-51 0.7813834259441919 1101 52-53 0.8084687109498958 1101 54-55 0.8444714747656406 1101 56-57 0.7755979739409398 1101 58-59 0.8646137508353391 1101 60-61 0.8190307250628521 1101 62-63 0.786274885311947 1101 64-65 0.7481692207719632 1101 66-67 0.7008507396168753 1101 68-69 0.6939503350797906 1101 70-71 0.7005784371338706 1101 72-73 0.6730223021995059 1101 74-75 0.670662222111666 1101 76-77 0.6154743385935966 1101 78-79 0.5624571755011374 1101 80-81 0.5581058297897243 1101 82-83 0.5611316822324035 1101 84-85 0.4974544212208052 1101 86-87 0.44568701708534064 1101 88-89 0.474555635831468 1101 90-91 0.4482519349312888 1101 92-93 0.42419777434841777 1101 94-95 0.4059965849936127 1101 96-97 0.3813573147244682 1101 98-99 0.3908538802157686 1101 100 0.3650483433916669 1102 1 0.6215063702137549 1102 2 0.6110094108727218 1102 3 0.601690366033548 1102 4 0.5409702796239841 1102 5 0.5330750614622346 1102 6 0.5633664678994634 1102 7 0.5568938723233359 1102 8 0.5697997920414437 1102 9 0.7021683159141361 1102 10-11 0.6562914533082136 1102 12-13 0.6579653434957891 1102 14-15 0.7780297368785583 1102 16-17 0.8240231607611079 1102 18-19 0.78602090838816 1102 20-21 0.7613835398839655 1102 22-23 0.7336424440400222 1102 24-25 0.7893801933770348 1102 26-27 0.7581169684554325 1102 28-29 0.7535238516674454 1102 30-31 0.7556819610468537 1102 32-33 0.7151013518675455 1102 34-35 0.6787323679995083 1102 36-37 0.7008178644875755 1102 38-39 0.76964129894818 1102 40-41 0.7284971330801469 1102 42-43 0.7097608695443611 1102 44-45 0.70355889467168 1102 46-47 0.77549485148635 1102 48-49 0.7880715270143028 1102 50-51 0.7156467243598001 1102 52-53 0.7973561688858979 1102 54-55 0.8961355785235767 1102 56-57 0.8541379432348677 1102 58-59 0.8445051325046009 1102 60-61 0.8379207499170889 1102 62-63 0.7820371168832025 1102 64-65 0.7251939067050799 1102 66-67 0.7398684294261315 1102 68-69 0.7357961785519223 1102 70-71 0.7205920213221546 1102 72-73 0.700965384608601 1102 74-75 0.666510660939565 1102 76-77 0.6781340132177043 1102 78-79 0.6331326274146534 1102 80-81 0.6133525630310999 1102 82-83 0.6223088694159173 1102 84-85 0.5521366003104546 1102 86-87 0.5248607612687408 1102 88-89 0.5589260953337707 1102 90-91 0.49050766606666585 1102 92-93 0.45803671828376125 1102 94-95 0.4937278139447052 1102 96-97 0.4315398092421354 1102 98-99 0.4167940464850908 1102 100 0.4002436146320534 1103 1 0.5804497681559688 1103 2 0.5737392800369285 1103 3 0.5691949209270568 1103 4 0.51967354636772 1103 5 0.5020794630540735 1103 6 0.4976460473273221 1103 7 0.5208191117441103 1103 8 0.5299667097507452 1103 9 0.6402125636847629 1103 10-11 0.6200118953140858 1103 12-13 0.6043499677986688 1103 14-15 0.7376317035948077 1103 16-17 0.7106897925567992 1103 18-19 0.7199203373798966 1103 20-21 0.6727130573669982 1103 22-23 0.7137399322154394 1103 24-25 0.728961111085944 1103 26-27 0.7453619078504161 1103 28-29 0.7194367892778928 1103 30-31 0.7318253303857816 1103 32-33 0.7220754307259938 1103 34-35 0.7013070742674685 1103 36-37 0.7248618272589553 1103 38-39 0.7681230101035723 1103 40-41 0.7246926087447534 1103 42-43 0.7310995047654458 1103 44-45 0.7249006449954649 1103 46-47 0.7987144720421924 1103 48-49 0.7889868759470957 1103 50-51 0.7586268483803025 1103 52-53 0.8170602994984435 1103 54-55 0.8868019754910605 1103 56-57 0.8823982088669808 1103 58-59 0.9179792595065877 1103 60-61 0.8738013049985156 1103 62-63 0.8293801594663108 1103 64-65 0.7927236837076848 1103 66-67 0.7509157080979705 1103 68-69 0.7610982055063715 1103 70-71 0.7911648859255038 1103 72-73 0.7770855925768316 1103 74-75 0.7514390475397903 1103 76-77 0.7196764889464085 1103 78-79 0.7205210762117531 1103 80-81 0.6944501669963294 1103 82-83 0.664862712787599 1103 84-85 0.6887746779446289 1103 86-87 0.654357992723348 1103 88-89 0.6415382639346952 1103 90-91 0.5922363524119021 1103 92-93 0.5585728016172631 1103 94-95 0.620609061947647 1103 96-97 0.5792595892787418 1103 98-99 0.5548312652550713 1103 100 0.5361679376163053 1104 1 0.516782030421691 1104 2 0.501013475858759 1104 3 0.47950277309358214 1104 4 0.4260062115831431 1104 5 0.42602284068819785 1104 6 0.4475801760004927 1104 7 0.4172739789812212 1104 8 0.45345350163451315 1104 9 0.5546193310389143 1104 10-11 0.5256166852406778 1104 12-13 0.5117183792592002 1104 14-15 0.6487715135131253 1104 16-17 0.6245716299739641 1104 18-19 0.6559551713894365 1104 20-21 0.5853047866529693 1104 22-23 0.5412088849269736 1104 24-25 0.5954643688540742 1104 26-27 0.6033809077855921 1104 28-29 0.6004386363695531 1104 30-31 0.5998569328129761 1104 32-33 0.6247657633961978 1104 34-35 0.6198595607069493 1104 36-37 0.6189086304960014 1104 38-39 0.6566418892346562 1104 40-41 0.6160827828597775 1104 42-43 0.618462270437 1104 44-45 0.6146147022531068 1104 46-47 0.6443445118670823 1104 48-49 0.6635322413211284 1104 50-51 0.5992755692988041 1104 52-53 0.6355705290546894 1104 54-55 0.6825121300062094 1104 56-57 0.7043290700476987 1104 58-59 0.7349267052490447 1104 60-61 0.7544323487700311 1104 62-63 0.7245654518864981 1104 64-65 0.6831617171133217 1104 66-67 0.6794435669388363 1104 68-69 0.66906096200006 1104 70-71 0.6779919863690012 1104 72-73 0.6862319132451589 1104 74-75 0.646542440686865 1104 76-77 0.643734845059786 1104 78-79 0.6136644744478339 1104 80-81 0.5678095790728825 1104 82-83 0.5644862593096001 1104 84-85 0.5754158055319003 1104 86-87 0.5820252456499446 1104 88-89 0.5945381260502032 1104 90-91 0.5418819597594542 1104 92-93 0.5395650561653689 1104 94-95 0.539718336107299 1104 96-97 0.538324647092594 1104 98-99 0.5022119681643282 1104 100 0.4838635092452961 1105 1 0.438031819102104 1105 2 0.41162658109679384 1105 3 0.411303596701746 1105 4 0.3836775169536324 1105 5 0.390231214388983 1105 6 0.4248477521741947 1105 7 0.4094092960015132 1105 8 0.41331254675139206 1105 9 0.48244621843461744 1105 10-11 0.47992844283889013 1105 12-13 0.4799886598666774 1105 14-15 0.568615522203757 1105 16-17 0.5390504925725068 1105 18-19 0.5646726709500953 1105 20-21 0.5449337628159086 1105 22-23 0.5486694971120016 1105 24-25 0.541144757654358 1105 26-27 0.5115060086934093 1105 28-29 0.5277622263848585 1105 30-31 0.556010817925241 1105 32-33 0.5306616819806749 1105 34-35 0.539851161805565 1105 36-37 0.5374689604060805 1105 38-39 0.5948202950164401 1105 40-41 0.5671059581148441 1105 42-43 0.5763264927741218 1105 44-45 0.5603422487815166 1105 46-47 0.5834687172365634 1105 48-49 0.5725954801083262 1105 50-51 0.5594474258612294 1105 52-53 0.5935471086826851 1105 54-55 0.6768746906267467 1105 56-57 0.6879646242527357 1105 58-59 0.6772754359343836 1105 60-61 0.6733309438033857 1105 62-63 0.6347851192075851 1105 64-65 0.6014632180071047 1105 66-67 0.5944654142535128 1105 68-69 0.5937089587023152 1105 70-71 0.6405171571341768 1105 72-73 0.6213357664356138 1105 74-75 0.5922877569194824 1105 76-77 0.624351961203363 1105 78-79 0.5946647184762526 1105 80-81 0.5903116853169905 1105 82-83 0.6236927209973082 1105 84-85 0.5836335975247344 1105 86-87 0.5572473275347853 1105 88-89 0.5605207670089492 1105 90-91 0.47429811807948496 1105 92-93 0.5100700350973675 1105 94-95 0.5686486754181956 1105 96-97 0.5030213373940846 1105 98-99 0.5191224048919487 1105 100 0.5365505317061938 1106 1 0.3388568495364872 1106 2 0.32595719402813117 1106 3 0.286463499726505 1106 4 0.29551879950083304 1106 5 0.29158387956035625 1106 6 0.3164089042932261 1106 7 0.29073966969700393 1106 8 0.31975742914856653 1106 9 0.3811061763378518 1106 10-11 0.38128135600553037 1106 12-13 0.36232220672740567 1106 14-15 0.39331090805612945 1106 16-17 0.41250058009512003 1106 18-19 0.404566128583177 1106 20-21 0.4035886324559925 1106 22-23 0.37066809242526233 1106 24-25 0.3834595707447406 1106 26-27 0.4058230192110557 1106 28-29 0.3932800456190009 1106 30-31 0.3817519363916162 1106 32-33 0.36364563380994497 1106 34-35 0.3124642650603775 1106 36-37 0.3391489710292248 1106 38-39 0.38663643910204826 1106 40-41 0.35976093755107996 1106 42-43 0.3589559888854055 1106 44-45 0.3214705976401504 1106 46-47 0.3988686163345534 1106 48-49 0.3708194436402934 1106 50-51 0.3609921429530587 1106 52-53 0.3942555972578887 1106 54-55 0.4199135077992082 1106 56-57 0.4158881400844976 1106 58-59 0.4680885429637911 1106 60-61 0.49111294474452194 1106 62-63 0.4624178025656178 1106 64-65 0.38794565630047373 1106 66-67 0.437780839813783 1106 68-69 0.4671909914761301 1106 70-71 0.4805534934934528 1106 72-73 0.460859705348696 1106 74-75 0.4628907952989394 1106 76-77 0.44839360499361547 1106 78-79 0.39462164296662294 1106 80-81 0.3712535401636785 1106 82-83 0.39553224280115273 1106 84-85 0.38281379967520834 1106 86-87 0.42587651042457964 1106 88-89 0.3628315034262002 1106 90-91 0.36786459917163583 1106 92-93 0.3636739862216878 1106 94-95 0.37231757927211007 1106 96-97 0.3373335565046922 1106 98-99 0.30133560922178404 1106 100 0.40049162008140016 1107 1 0.44997046819478115 1107 2 0.43231855960660326 1107 3 0.37104391629686617 1107 4 0.39066036367067625 1107 5 0.4267083595452945 1107 6 0.40867364458996036 1107 7 0.39474807505214926 1107 8 0.43913344901798723 1107 9 0.523010311165848 1107 10-11 0.49048580754956106 1107 12-13 0.4722527439661022 1107 14-15 0.5727064873634689 1107 16-17 0.5823799644161056 1107 18-19 0.5576288103812388 1107 20-21 0.5429629953021475 1107 22-23 0.5105780623630523 1107 24-25 0.5727192090330533 1107 26-27 0.5619624590277681 1107 28-29 0.5347013208926228 1107 30-31 0.5106132557015641 1107 32-33 0.5096426294169873 1107 34-35 0.4921352055800199 1107 36-37 0.5297492815290994 1107 38-39 0.5972517537574547 1107 40-41 0.5728592510722095 1107 42-43 0.5782728508251651 1107 44-45 0.5457812872857701 1107 46-47 0.5729010793728264 1107 48-49 0.5656178606637496 1107 50-51 0.5169216683014923 1107 52-53 0.5603416551468996 1107 54-55 0.6229960497983811 1107 56-57 0.6234114187301785 1107 58-59 0.642623303796249 1107 60-61 0.6548237641832557 1107 62-63 0.6149441945828258 1107 64-65 0.615043948868383 1107 66-67 0.5637278645171548 1107 68-69 0.6380307580571127 1107 70-71 0.6384552058883983 1107 72-73 0.6331919006117985 1107 74-75 0.6261597557168415 1107 76-77 0.6396234677453485 1107 78-79 0.664690742228558 1107 80-81 0.6452513104793169 1107 82-83 0.5858249620339002 1107 84-85 0.612348664680102 1107 86-87 0.5979384141848918 1107 88-89 0.6566776316781286 1107 90-91 0.6392487490219345 1107 92-93 0.6406074662142345 1107 94-95 0.6316774918552142 1107 96-97 0.614020509702982 1107 98-99 0.6126633757338844 1107 100 0.6517530647250176 1108 1 0.20268273276019144 1108 2 0.17365771682831266 1108 3 0.17539981716710784 1108 4 0.20502786704145137 1108 5 0.19401748004257513 1108 6 0.21710361768268882 1108 7 0.205233628458366 1108 8 0.2002165335616226 1108 9 0.24779380811057905 1108 10-11 0.22912293287245689 1108 12-13 0.19497885249656832 1108 14-15 0.26125594198506263 1108 16-17 0.2762371524960088 1108 18-19 0.2760837194098116 1108 20-21 0.22466593373800947 1108 22-23 0.19220314783657955 1108 24-25 0.20764419434214432 1108 26-27 0.22326197693747218 1108 28-29 0.19889521749195893 1108 30-31 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98-99 -1.5146628326033174 2316 100 -1.6723280924149009 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 35.0 4 85.0 5 187.0 6 1437.0 7 17981.0 8 24449.0 9 32552.0 10 36905.0 11 41112.0 12 53828.0 13 66934.0 14 100794.0 15 171404.0 16 224870.0 17 265490.0 18 257425.0 19 247094.0 20 257652.0 21 302773.0 22 403282.0 23 555579.0 24 676586.0 25 737360.0 26 698647.0 27 605820.0 28 558659.0 29 574551.0 30 638921.0 31 741199.0 32 897441.0 33 1134467.0 34 1524541.0 35 2223560.0 36 3599127.0 37 6476427.0 38 8547144.0 39 2914592.0 40 7497.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.646523849330734 37.99269229616517 26.567837232740644 18.792946621763452 2 19.97722797471058 35.99533361334349 26.182923569975607 17.844514841970323 3 26.521913793146044 27.524781230437423 25.824068144649477 20.129236831767056 4 18.889766994494945 28.2897606999854 25.528585088868393 27.29188721665126 5 19.62462929039376 35.72768296725807 25.418087102495086 19.22960063985309 6 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19.968523816976617 27.72069478504568 32.57725827311709 19.733523124860618 54-55 20.486454936362858 27.889690876268997 31.807252732658487 19.816601454709655 56-57 21.10667480214906 28.269549727504263 30.730256059454607 19.89351941089207 58-59 21.552297914763376 28.688570103235005 29.967469996328607 19.791661985673016 60-61 21.920210870602375 28.948227614659043 29.40151923659525 19.730042278143333 62-63 22.259895235063052 29.048251736835663 29.047110953461967 19.64474207463932 64-65 22.681152064879342 29.043809612999095 28.748950014130976 19.526088307990587 66-67 23.08953446028241 28.85259566416693 28.59657991293107 19.461289962619592 68-69 23.477293092841713 28.596733643639062 28.541807001823074 19.384166261696155 70-71 23.85950160320863 28.401196930242424 28.5148730855955 19.224428380953444 72-73 24.280976927590622 28.28417084568563 28.332754894946195 19.102097331777554 74-75 24.618003745898754 28.33559716984903 28.077090887355094 18.96930819689712 76-77 24.981138698323626 28.524429987144522 27.665515866379636 18.828915448152213 78-79 25.289889686698437 28.736736747645743 27.217486083827445 18.755887481828374 80-81 25.58533242174665 28.85892581868554 26.868115939322006 18.6876258202458 82-83 25.827076154472113 28.970857030058838 26.581254719356355 18.620812096112694 84-85 25.95906662933036 29.02108363892395 26.409034892624263 18.61081483912143 86-87 26.08107685045047 28.97018075828791 26.315897917120708 18.63284447414091 88-89 26.208984756407556 28.87271252602519 26.292094568544126 18.626208149023128 90-91 26.359275263314103 28.696376447193995 26.24394316148392 18.700405128007986 92-93 26.5116798013396 28.57286657105444 26.230298166162637 18.685155461443326 94-95 26.721947158737347 28.5176523199892 26.135235727217832 18.625164794055625 96-97 26.843015127741733 28.407612816466575 26.03660936645334 18.71276268933835 98-99 26.93622132539799 28.323646149437625 25.889830467411613 18.85030205775277 100 26.935990419075555 28.354036338018318 25.747431067582905 18.96254217532322 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 75222.0 1 47435.5 2 16717.5 3 13238.5 4 12792.0 5 13259.5 6 14055.0 7 14758.5 8 15511.5 9 16437.5 10 18264.5 11 26901.5 12 127121.5 13 154077.0 14 87951.5 15 93714.5 16 105588.0 17 117436.5 18 128792.0 19 140298.0 20 155940.0 21 179660.5 22 211963.5 23 247710.0 24 283757.5 25 324911.5 26 370719.0 27 424577.0 28 498368.0 29 574045.5 30 635836.5 31 699507.0 32 768531.5 33 828223.5 34 878127.0 35 944731.0 36 1023768.0 37 1075498.0 38 1112582.5 39 1142768.5 40 1153579.0 41 1166684.0 42 1199000.0 43 1208732.0 44 1201910.0 45 1209429.0 46 1211137.0 47 1210344.5 48 1189095.0 49 1150285.0 50 1117125.5 51 1080390.5 52 1031725.5 53 969557.0 54 893191.0 55 811469.5 56 730435.0 57 651124.0 58 571539.0 59 486146.0 60 400819.0 61 327903.5 62 260908.0 63 196465.5 64 144650.5 65 105760.5 66 77076.0 67 57848.0 68 44207.5 69 34293.0 70 27354.5 71 22316.0 72 18841.0 73 15413.5 74 12648.0 75 11198.5 76 8943.0 77 5929.5 78 4062.0 79 3130.5 80 2405.5 81 1897.0 82 1519.5 83 1184.0 84 958.0 85 779.0 86 619.0 87 531.0 88 460.0 89 392.0 90 337.5 91 290.0 92 253.5 93 208.5 94 180.5 95 150.5 96 115.5 97 88.5 98 65.5 99 56.5 100 44.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04716662370666942 2 0.015197198459773902 3 0.005432584337643174 4 1.796823760254073E-4 5 6.485410759667046E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.018842813492473E-6 12-13 3.369044550476387E-5 14-15 1.4037685626984946E-6 16-17 0.0 18-19 0.0 20-21 0.038843679898430045 22-23 0.07585544182253856 24-25 0.0319806553953971 26-27 0.036408141442148156 28-29 0.041056019153242876 30-31 0.03451165011394249 32-33 0.03523178338660682 34-35 0.036200383694868776 36-37 0.03381397713828133 38-39 0.044193441890874006 40-41 0.06431084916290614 42-43 0.07261133267414233 44-45 0.07546519416210837 46-47 0.04882307061065364 48-49 0.031028900309887527 50-51 0.028513347045531823 52-53 0.028676184198804848 54-55 0.02960688275587395 56-57 0.02976691237202158 58-59 0.037708031131206965 60-61 0.057449228428435896 62-63 0.08093568025094441 64-65 0.1088243502860754 66-67 0.0901780924677513 68-69 0.05364361185496027 70-71 0.04726067620037022 72-73 0.05122491862143076 74-75 0.048677078680133004 76-77 0.0516544718016165 78-79 0.04451490489173196 80-81 0.04290899365600489 82-83 0.042211320680343736 84-85 0.04962041115426639 86-87 0.05323230766608962 88-89 0.0587491181174947 90-91 0.05588543024958977 92-93 0.05154076654803793 94-95 0.05348217847024995 96-97 0.05531690398169688 98-99 0.05010751884552277 100 0.051945051894095096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.5618407E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.309743760219156 #Duplication Level Percentage of deduplicated Percentage of total 1 72.40438631531886 29.18602259486422 2 14.570646495964667 11.746780533461418 3 4.928995685795337 5.960596592689072 4 2.2424443701008134 3.6156943182123946 5 1.28814804862765 2.5962458882703454 6 0.8726338010280233 2.110538694956742 7 0.6090687433107357 1.7185983482650116 8 0.45012083164425226 1.4515404307773248 9 0.3431870542671502 1.2450403997399928 >10 1.9569147150714685 14.459919864026677 >50 0.1774394394158233 5.001876813677394 >100 0.1487123338229217 10.787696619133353 >500 0.006379051484115792 1.5740547571153076 >1k 7.576928016132399E-4 0.7402369025128702 >5k 1.102808985943422E-4 0.2639592051141115 >10k+ 5.514044781630247E-5 7.54119803718384 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2325008 6.527546276845003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 222147 0.623685949795565 No Hit AAGCAGTGGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA 81861 0.22982779662212294 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002394829167963632 1.1230148501587957E-5 0.0 5.615074250793978E-6 1.1230148501587957E-5 2 0.002394829167963632 1.6845222752381934E-5 0.0 5.615074250793978E-6 1.1230148501587957E-5 3 0.0023976367050890287 1.6845222752381934E-5 0.0 1.1230148501587957E-5 1.1230148501587957E-5 4 0.0023976367050890287 2.2460297003175914E-5 0.0 1.9652759877778926E-5 1.1230148501587957E-5 5 0.002400444242214426 2.8075371253969893E-5 0.0 1.9652759877778926E-5 1.1230148501587957E-5 6 0.0024032517793398226 3.649798263016086E-5 0.0 1.9652759877778926E-5 1.1230148501587957E-5 7 0.0024032517793398226 3.649798263016086E-5 0.0 1.9652759877778926E-5 1.6845222752381934E-5 8 0.0024032517793398226 3.649798263016086E-5 0.0 1.9652759877778926E-5 1.6845222752381934E-5 9 0.002408866853590617 3.930551975555785E-5 0.0 1.9652759877778926E-5 1.6845222752381934E-5 10-11 0.0024102706221533154 3.930551975555785E-5 0.0 2.105652844047742E-5 2.105652844047742E-5 12-13 0.0024200970020922044 3.930551975555785E-5 0.0 2.52678341285729E-5 2.6671602691271397E-5 14-15 0.0024706326703493506 4.211305688095484E-5 0.0 2.947913981666839E-5 2.947913981666839E-5 16-17 0.0025899529981787224 5.895827963333677E-5 0.0 3.228667694206537E-5 3.228667694206537E-5 18-19 0.0032553392968978087 7.72072709484172E-5 0.0 3.5094214067462364E-5 3.7901751192859356E-5 20-21 0.003436425441485915 9.264872513810065E-5 0.0 3.649798263016086E-5 5.474697394524129E-5 22-23 0.0038940539929256243 1.0668641076508559E-4 0.0 3.930551975555785E-5 6.878465957222623E-5 24-25 0.0051911361448590335 1.1089771645318108E-4 2.807537125396989E-6 4.351682544365333E-5 7.580350238571871E-5 26-27 0.007518584421813137 1.1510902214127656E-4 2.807537125396989E-6 4.9131899694447314E-5 8.14185766365127E-5 28-29 0.014246847142827021 1.1932032782937205E-4 2.807537125396989E-6 5.053566825714581E-5 8.282234519921118E-5 30-31 0.030593732055450993 1.33358013456357E-4 2.807537125396989E-6 5.053566825714581E-5 8.703365088730666E-5 32-33 0.06424487204045931 1.3756931914445247E-4 2.807537125396989E-6 6.176581675873377E-5 9.124495657540215E-5 34-35 0.12119576262913724 1.4739569908334192E-4 2.807537125396989E-6 6.316958532143226E-5 1.038788736396886E-4 36-37 0.20979180792672733 1.6424092183572387E-4 2.807537125396989E-6 6.457335388413075E-5 1.1510902214127656E-4 38-39 0.33279281692749485 1.796823760254073E-4 2.807537125396989E-6 6.597712244682924E-5 1.2633917064286453E-4 40-41 0.49467119627219713 1.8950875596429677E-4 2.807537125396989E-6 7.018842813492473E-5 1.3195424489365848E-4 42-43 0.6970665476420661 2.021426730285832E-4 2.807537125396989E-6 7.439973382302022E-5 1.3897308770715098E-4 44-45 0.94166339331234 2.1898789578096516E-4 2.807537125396989E-6 7.580350238571871E-5 1.4599193052064344E-4 46-47 1.2297181061466338 2.3302558140795012E-4 2.807537125396989E-6 7.580350238571871E-5 1.6424092183572387E-4 48-49 1.5601708408801103 2.470632670349351E-4 2.807537125396989E-6 8.282234519921118E-5 1.6845222752381934E-4 50-51 1.9336434669860447 2.58293415536523E-4 2.807537125396989E-6 9.686003082619613E-5 1.6985599608651785E-4 52-53 2.345444589927899 2.66716026912714E-4 2.807537125396989E-6 1.038788736396886E-4 1.7406730177461333E-4 54-55 2.7969723631941203 2.723311011635079E-4 2.807537125396989E-6 1.052826422023871E-4 1.8389368171350278E-4 56-57 3.2839116022229744 2.7794617541430195E-4 2.807537125396989E-6 1.0668641076508559E-4 1.9933513590318625E-4 58-59 3.804527249070965 2.8636878679049295E-4 2.807537125396989E-6 1.0668641076508559E-4 2.0354644159128172E-4 60-61 4.358561010322556 2.9338762960398536E-4 2.807537125396989E-6 1.0668641076508559E-4 2.063539787166787E-4 62-63 4.938970179098689 3.0181024098017636E-4 2.807537125396989E-6 1.1089771645318108E-4 2.2179543290636215E-4 64-65 5.546816004432764 3.2707807510874926E-4 2.807537125396989E-6 1.1370525357857807E-4 2.2741050715715613E-4 66-67 6.17674451302665 3.397119921730357E-4 2.807537125396989E-6 1.2072409639207055E-4 2.372368870960456E-4 68-69 6.83336708460881 3.453270664238297E-4 2.807537125396989E-6 1.2633917064286453E-4 2.4144819278414106E-4 70-71 7.5098557888902775 3.453270664238297E-4 2.807537125396989E-6 1.27742939205563E-4 2.4565949847223654E-4 72-73 8.205284138619675 3.4813460354922665E-4 2.807537125396989E-6 1.291467077682615E-4 2.55485878411126E-4 74-75 8.920431225349297 3.6076852061351315E-4 2.807537125396989E-6 1.3055047633095997E-4 2.568896469738245E-4 76-77 9.649105025949083 3.8603635474208605E-4 2.807537125396989E-6 1.3195424489365848E-4 2.653122583500155E-4 78-79 10.394002179827975 3.958627346809755E-4 8.422611376190967E-6 1.3476178201905548E-4 2.6811979547541247E-4 80-81 11.156258335753197 4.127079574333574E-4 8.422611376190967E-6 1.3476178201905548E-4 2.7794617541430195E-4 82-83 11.929810617302453 4.435908658127243E-4 8.422611376190967E-6 1.3756931914445247E-4 2.8777255535319143E-4 84-85 12.713488281494453 4.716662370666942E-4 8.422611376190967E-6 1.3756931914445247E-4 2.9759893529208086E-4 86-87 13.512394027054608 5.123755253849505E-4 8.422611376190967E-6 1.3756931914445247E-4 2.9759893529208086E-4 88 14.119264233237606 5.474697394524129E-4 8.422611376190967E-6 1.3756931914445247E-4 2.9759893529208086E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 301840 0.0 87.28114 8 GTGGTAT 304195 0.0 86.750465 6 AGTGGTA 305080 0.0 86.530205 5 TGGTATC 305130 0.0 86.17226 7 GTATCAA 307375 0.0 85.61317 9 AAGCAGT 310930 0.0 85.25913 1 GCAGTGG 313150 0.0 84.75264 3 CAGTGGT 318395 0.0 82.73751 4 AGCAGTG 321840 0.0 82.558624 2 ATCAACG 302490 0.0 44.56079 10-11 CAACGCA 301900 0.0 44.493008 12-13 AGAGTAC 287075 0.0 44.300755 18-19 ACGCAGA 306005 0.0 43.845005 14-15 TCAACGC 300880 0.0 43.82112 12-13 GAGTACT 282830 0.0 43.74257 20-21 AACGCAG 304225 0.0 43.326153 14-15 TATCAAC 307405 0.0 42.727535 10-11 CGCAGAG 308650 0.0 42.58215 16-17 AGTACTT 296610 0.0 42.388172 20-21 GTACTTT 293215 0.0 42.180294 22-23 >>END_MODULE