FastQCFastQC Report
Thu 26 May 2016
SRR1660760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35618407
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25475657.152383316861981No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790230.5026137187999452No Hit
AAGCAGTGGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA499580.1402589397105828No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT474380.13318394615458237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3260500.087.662898
GTGGTAT3290200.087.20156
AGTGGTA3300100.086.9861765
TGGTATC3297650.086.662487
GTATCAA3317800.085.993119
AAGCAGT3288500.085.647631
GCAGTGG3366300.085.334193
CAGTGGT3426050.083.5441744
AGCAGTG3453800.083.057992
ATCAACG3259450.044.6911610-11
CAACGCA3258700.044.65031412-13
AGAGTAC3191400.044.1864218-19
ACGCAGA3313850.043.97873314-15
TCAACGC3246150.043.9709812-13
GAGTACT3149000.043.6745620-21
AACGCAG3282500.043.5245714-15
TATCAAC3320600.042.9284510-11
CGCAGAG3338800.042.84343716-17
AGTACTT3295600.042.43131620-21
GTACTTT3257000.042.17766622-23
Produced by FastQC (version 0.11.5)