##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660760_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35618407 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.997622521411472 33.0 31.0 34.0 27.0 34.0 2 31.78349045761648 34.0 31.0 34.0 28.0 34.0 3 32.288884227753364 34.0 31.0 34.0 30.0 34.0 4 35.815607924295996 37.0 35.0 37.0 35.0 37.0 5 35.87117363221775 37.0 35.0 37.0 35.0 37.0 6 35.91053819447905 37.0 35.0 37.0 35.0 37.0 7 35.86840641132547 37.0 35.0 37.0 35.0 37.0 8 35.90388770053641 37.0 35.0 37.0 35.0 37.0 9 37.621104503634875 39.0 37.0 39.0 35.0 39.0 10-11 37.520269014276806 39.0 37.0 39.0 34.5 39.0 12-13 37.632252096507294 39.0 37.0 39.0 35.0 39.0 14-15 38.97270943363638 40.0 38.0 41.0 35.0 41.0 16-17 38.97338756615365 40.0 38.0 41.0 35.0 41.0 18-19 38.89922921033498 40.0 38.0 41.0 35.0 41.0 20-21 38.790638699816085 40.0 38.0 41.0 34.5 41.0 22-23 38.62619873763585 40.0 38.0 41.0 34.5 41.0 24-25 38.58395406341446 40.0 38.0 41.0 34.0 41.0 26-27 38.554919454988536 40.0 38.0 41.0 34.0 41.0 28-29 38.43223246901525 40.0 38.0 41.0 34.0 41.0 30-31 38.298384708782734 40.0 38.0 41.0 34.0 41.0 32-33 38.152178492991 40.0 38.0 41.0 33.5 41.0 34-35 37.99624243442443 40.0 38.0 41.0 33.0 41.0 36-37 37.745480096849924 40.0 37.5 41.0 33.0 41.0 38-39 37.193518550675215 40.0 35.0 41.0 32.5 41.0 40-41 37.015334571251316 39.5 35.0 41.0 31.5 41.0 42-43 36.826781683414424 39.0 35.0 41.0 31.0 41.0 44-45 36.69315803483295 39.0 35.0 41.0 31.0 41.0 46-47 36.543182756601105 39.0 35.0 41.0 30.0 41.0 48-49 36.62013285714883 39.0 35.0 41.0 31.0 41.0 50-51 36.485277808746474 39.0 35.0 41.0 30.0 41.0 52-53 36.175566498524205 39.0 35.0 41.0 28.5 41.0 54-55 35.639824024134484 39.0 35.0 41.0 26.0 41.0 56-57 35.302042957732496 39.0 35.0 41.0 23.0 41.0 58-59 35.08033277569095 39.0 35.0 41.0 22.0 41.0 60-61 34.85294801364923 39.0 35.0 41.0 21.5 41.0 62-63 34.53800280849168 38.0 34.0 40.5 20.0 41.0 64-65 34.26535908806927 37.5 34.0 40.0 19.5 41.0 66-67 33.97647748536312 37.0 34.0 40.0 19.0 41.0 68-69 33.664593169481165 36.5 33.5 40.0 19.0 41.0 70-71 33.32072708922665 36.0 33.0 39.0 17.5 41.0 72-73 32.98837769471274 35.5 33.0 39.0 17.5 41.0 74-75 32.62587991371989 35.0 33.0 38.5 16.0 40.0 76-77 30.583833156828153 33.5 30.0 35.5 10.5 38.5 78-79 31.79873973588993 35.0 32.0 37.0 12.5 39.0 80-81 31.776867140633215 35.0 32.0 36.5 12.5 39.0 82-83 31.55281795729944 35.0 32.0 36.0 11.5 38.0 84-85 31.318912577982502 35.0 32.0 36.0 10.5 37.0 86-87 31.11630598751932 35.0 32.0 35.0 10.0 37.0 88-89 30.897463788877474 35.0 32.0 35.0 10.0 36.0 90-91 30.735551241805958 35.0 32.0 35.0 10.0 36.0 92-93 30.6073754365264 35.0 32.0 35.0 10.0 36.0 94-95 30.50392893764171 35.0 31.5 35.0 10.0 36.0 96-97 30.394696497796772 35.0 31.0 35.0 10.0 35.0 98-99 30.27927736914231 35.0 31.0 35.0 10.0 35.0 100 30.096233332389065 35.0 31.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7467835679581256 1101 2 1.206972836809765 1101 3 0.7659383133297624 1101 4 0.592835091460131 1101 5 0.4745814066827876 1101 6 0.4113693912998855 1101 7 0.39861355705318857 1101 8 0.41824898127401866 1101 9 0.4954814619653831 1101 10-11 0.5065678929128907 1101 12-13 0.45181111823759323 1101 14-15 0.6101008043258886 1101 16-17 0.5951101152410203 1101 18-19 0.5520363349335327 1101 20-21 0.5805600739230172 1101 22-23 0.5729118838643714 1101 24-25 0.6213736167697661 1101 26-27 0.6113862641874306 1101 28-29 0.6123013076938335 1101 30-31 0.5831770616797627 1101 32-33 0.5767898385440802 1101 34-35 0.5881662741068112 1101 36-37 0.5901274085179935 1101 38-39 0.6870435413466325 1101 40-41 0.6790025183417114 1101 42-43 0.6585817424381872 1101 44-45 0.6415023494292029 1101 46-47 0.5831724973791239 1101 48-49 0.5724087976134484 1101 50-51 0.5720663871329208 1101 52-53 0.5693532885253916 1101 54-55 0.6099839127368512 1101 56-57 0.6464450717833117 1101 58-59 0.6445767647061942 1101 60-61 0.6040474825493547 1101 62-63 0.6655632940136513 1101 64-65 0.6287769860980532 1101 66-67 0.5862477631161838 1101 68-69 0.6181385719687995 1101 70-71 0.6130323160374687 1101 72-73 0.5869777617636132 1101 74-75 0.5681603362636238 1101 76-77 0.5167002035994201 1101 78-79 0.6295365842160372 1101 80-81 0.5961039480782375 1101 82-83 0.5744283395421057 1101 84-85 0.5804595643944275 1101 86-87 0.5583760350792417 1101 88-89 0.5975630733930011 1101 90-91 0.6168161398827507 1101 92-93 0.5737466156704478 1101 94-95 0.5221793822007186 1101 96-97 0.5031458170926086 1101 98-99 0.49003450486369715 1101 100 0.42192882413189636 1102 1 1.6543173640692217 1102 2 1.1347371340877395 1102 3 0.7085085595721097 1102 4 0.5562587746422665 1102 5 0.40950341070399077 1102 6 0.38063489601804434 1102 7 0.362362590269818 1102 8 0.37009220319098546 1102 9 0.46615152768325174 1102 10-11 0.4416570497257837 1102 12-13 0.37863599549212523 1102 14-15 0.5396065145191145 1102 16-17 0.5244488402037675 1102 18-19 0.5101209475698738 1102 20-21 0.514589669316166 1102 22-23 0.5301916042013417 1102 24-25 0.5614417595502701 1102 26-27 0.5494947709942011 1102 28-29 0.5716744601443438 1102 30-31 0.5753062413044532 1102 32-33 0.5455997413069866 1102 34-35 0.5434012478417642 1102 36-37 0.608265804596364 1102 38-39 0.6664727397944361 1102 40-41 0.6404018644771199 1102 42-43 0.623499619905246 1102 44-45 0.5995585275245645 1102 46-47 0.6209468305569672 1102 48-49 0.6174568736808794 1102 50-51 0.6202008436674333 1102 52-53 0.5547130561136697 1102 54-55 0.5975633354096814 1102 56-57 0.6656726292705386 1102 58-59 0.650897130620173 1102 60-61 0.597457126135744 1102 62-63 0.6372806607564883 1102 64-65 0.6327246117980323 1102 66-67 0.5909781181517246 1102 68-69 0.6025202981679953 1102 70-71 0.6009384621483917 1102 72-73 0.5656370956878902 1102 74-75 0.560003445495667 1102 76-77 0.481225384885402 1102 78-79 0.6158889680275657 1102 80-81 0.6246777563609953 1102 82-83 0.5834162651522483 1102 84-85 0.5931916051197987 1102 86-87 0.5748003396980756 1102 88-89 0.6016781496050534 1102 90-91 0.6044638706831051 1102 92-93 0.554730107526872 1102 94-95 0.50684110021 1102 96-97 0.4882421963926191 1102 98-99 0.5564163366025241 1102 100 0.47772719883830916 1103 1 1.587626721050576 1103 2 1.0609575310850623 1103 3 0.6309260778147134 1103 4 0.520862791249435 1103 5 0.37860761768738627 1103 6 0.36355174949148505 1103 7 0.3462676525712496 1103 8 0.3647909458401273 1103 9 0.44244442621117486 1103 10-11 0.4021833557259171 1103 12-13 0.37356122342932707 1103 14-15 0.5142798066940131 1103 16-17 0.46343082901504573 1103 18-19 0.4257570618772064 1103 20-21 0.4371718663128519 1103 22-23 0.47549414490636366 1103 24-25 0.5436776677599013 1103 26-27 0.482645584298389 1103 28-29 0.5108170087885924 1103 30-31 0.4729720877535044 1103 32-33 0.4799531574717193 1103 34-35 0.47265759922380823 1103 36-37 0.5001914126839964 1103 38-39 0.5775989890173463 1103 40-41 0.5713509135393551 1103 42-43 0.5064026340309056 1103 44-45 0.4997198026668954 1103 46-47 0.5482338520645555 1103 48-49 0.5191257846981117 1103 50-51 0.4725166700781642 1103 52-53 0.48766285442482626 1103 54-55 0.5640189156751703 1103 56-57 0.6057695082261461 1103 58-59 0.553309193826685 1103 60-61 0.5229474986737515 1103 62-63 0.5674298227211736 1103 64-65 0.5480694015306327 1103 66-67 0.5395352894753671 1103 68-69 0.5617344204000361 1103 70-71 0.5902132203513943 1103 72-73 0.5296087975611243 1103 74-75 0.5620375465050245 1103 76-77 0.44341582588523565 1103 78-79 0.5431615768608147 1103 80-81 0.594611347369252 1103 82-83 0.5881289853268221 1103 84-85 0.5978067873681887 1103 86-87 0.5678926250215817 1103 88-89 0.6125482394180359 1103 90-91 0.5872917577321708 1103 92-93 0.5486141443333103 1103 94-95 0.5008841804730721 1103 96-97 0.4931458635596684 1103 98-99 0.5387833048781481 1103 100 0.4823502753305462 1104 1 1.573261278373316 1104 2 1.0672493218318273 1104 3 0.627768910363514 1104 4 0.5046513171739306 1104 5 0.36510102848658477 1104 6 0.3373262499284735 1104 7 0.28040703217395446 1104 8 0.30751533224415795 1104 9 0.391314995182384 1104 10-11 0.3775979106295466 1104 12-13 0.3321171982811961 1104 14-15 0.48542307925539774 1104 16-17 0.4677761315421378 1104 18-19 0.4030873070270644 1104 20-21 0.38189655502949904 1104 22-23 0.4104464336570075 1104 24-25 0.4761624494825085 1104 26-27 0.4490339034577744 1104 28-29 0.46919748456380006 1104 30-31 0.45554116826149027 1104 32-33 0.4600504850528111 1104 34-35 0.4341849693551936 1104 36-37 0.4516918269386352 1104 38-39 0.5342785056751254 1104 40-41 0.5382407802225302 1104 42-43 0.5025008732028837 1104 44-45 0.47100451565167134 1104 46-47 0.5268902126813089 1104 48-49 0.49118714997142376 1104 50-51 0.44338587148899933 1104 52-53 0.4455638717397079 1104 54-55 0.45355758885016684 1104 56-57 0.5039289997375462 1104 58-59 0.5072039167543565 1104 60-61 0.5151880135110858 1104 62-63 0.5750129967451656 1104 64-65 0.5254209227939768 1104 66-67 0.5366367742083398 1104 68-69 0.5431933650764265 1104 70-71 0.5686580266046164 1104 72-73 0.5151036700062264 1104 74-75 0.4599591606783662 1104 76-77 0.44636867294028093 1104 78-79 0.5184670983427715 1104 80-81 0.4997114294344982 1104 82-83 0.45311179039992666 1104 84-85 0.47440201658903547 1104 86-87 0.4903205466506009 1104 88-89 0.5353714709690145 1104 90-91 0.5264114688022659 1104 92-93 0.4987968230326878 1104 94-95 0.5212511210419919 1104 96-97 0.46879483443699144 1104 98-99 0.4984139720961984 1104 100 0.42713556234326333 1105 1 1.556046951954798 1105 2 1.0251298410703278 1105 3 0.6020060809219956 1105 4 0.4885809478819425 1105 5 0.34017792013288783 1105 6 0.2799104377500967 1105 7 0.2310348295520228 1105 8 0.26897555565211917 1105 9 0.3294054628431127 1105 10-11 0.2994731993300306 1105 12-13 0.27272442332508007 1105 14-15 0.38948749990500886 1105 16-17 0.31185202519382926 1105 18-19 0.33304130516053476 1105 20-21 0.3398747340421977 1105 22-23 0.33251838194205163 1105 24-25 0.3789477264462775 1105 26-27 0.34583561959598796 1105 28-29 0.36914100331757993 1105 30-31 0.3660664207284654 1105 32-33 0.3798648554420012 1105 34-35 0.36244967749030366 1105 36-37 0.3733551866516791 1105 38-39 0.4385630435516319 1105 40-41 0.4506525830209469 1105 42-43 0.4276911578024709 1105 44-45 0.43318755539231546 1105 46-47 0.46435338343655985 1105 48-49 0.43285607948501337 1105 50-51 0.4436591485690826 1105 52-53 0.4321659683225221 1105 54-55 0.49025339321375583 1105 56-57 0.46683224170955384 1105 58-59 0.4591536004364656 1105 60-61 0.42810012561540844 1105 62-63 0.475541388508681 1105 64-65 0.4264824399060032 1105 66-67 0.4051128240074746 1105 68-69 0.4559727583495743 1105 70-71 0.4756202680418937 1105 72-73 0.42380989594570906 1105 74-75 0.3776327354681399 1105 76-77 0.35223432155087764 1105 78-79 0.462568988448691 1105 80-81 0.42531718090864246 1105 82-83 0.45315684777597554 1105 84-85 0.49960426849498774 1105 86-87 0.4781250778889756 1105 88-89 0.5184060255506182 1105 90-91 0.524684741904295 1105 92-93 0.5133253702140159 1105 94-95 0.46302016939922197 1105 96-97 0.49293352726319384 1105 98-99 0.5055197571355698 1105 100 0.45508295915016816 1106 1 1.4125410584141065 1106 2 0.8882945773683382 1106 3 0.4856197712934929 1106 4 0.45869249793084066 1106 5 0.27316077870673183 1106 6 0.24145601431278862 1106 7 0.19491702677876788 1106 8 0.204614109065119 1106 9 0.2670304860540327 1106 10-11 0.22829454385800574 1106 12-13 0.2006029071375437 1106 14-15 0.3300158810270233 1106 16-17 0.2937957087253409 1106 18-19 0.22937708138108803 1106 20-21 0.22476084662058327 1106 22-23 0.2434208086089953 1106 24-25 0.29492234270237105 1106 26-27 0.28388084022549265 1106 28-29 0.30845339672848837 1106 30-31 0.28881305767095 1106 32-33 0.2867769401659288 1106 34-35 0.28254679179219266 1106 36-37 0.32845408809976107 1106 38-39 0.40233624397342993 1106 40-41 0.4207406877123461 1106 42-43 0.3673713904863476 1106 44-45 0.35030658907798795 1106 46-47 0.3941413293249312 1106 48-49 0.39488144384606727 1106 50-51 0.3775992215939681 1106 52-53 0.3337150469014034 1106 54-55 0.37545321404167 1106 56-57 0.3809603936091932 1106 58-59 0.29237780316275774 1106 60-61 0.32078511617044825 1106 62-63 0.39131068544340764 1106 64-65 0.3719980555118454 1106 66-67 0.37259671306395603 1106 68-69 0.3722809616946918 1106 70-71 0.3820482348547003 1106 72-73 0.34988623388670703 1106 74-75 0.3334293652650189 1106 76-77 0.27533575667551347 1106 78-79 0.4029303810903748 1106 80-81 0.3586698938612045 1106 82-83 0.38466028191412605 1106 84-85 0.3765969903690163 1106 86-87 0.38231422299111983 1106 88-89 0.4183924559415111 1106 90-91 0.4156279278742474 1106 92-93 0.4079172194713543 1106 94-95 0.3794885188621322 1106 96-97 0.39529744101787756 1106 98-99 0.4627294974328713 1106 100 0.4111949893317508 1107 1 1.5395961874515578 1107 2 1.0078454559114753 1107 3 0.5878544289551044 1107 4 0.49797251930084485 1107 5 0.3362063990682742 1107 6 0.3184750577041484 1107 7 0.2597240446890581 1107 8 0.27156601539770975 1107 9 0.32680126879148474 1107 10-11 0.2901916114397167 1107 12-13 0.27249515870894925 1107 14-15 0.4016158003257928 1107 16-17 0.35612153956773795 1107 18-19 0.3287441035728307 1107 20-21 0.3454895575936803 1107 22-23 0.3740664115848773 1107 24-25 0.42721862290857615 1107 26-27 0.38344879506996676 1107 28-29 0.36593386636719316 1107 30-31 0.38481907379905067 1107 32-33 0.38646511361361746 1107 34-35 0.42116787179248405 1107 36-37 0.45021798151280024 1107 38-39 0.46283348165137994 1107 40-41 0.4626189470754767 1107 42-43 0.44340174910364283 1107 44-45 0.4326478716825761 1107 46-47 0.4943815941374936 1107 48-49 0.49356469045270046 1107 50-51 0.49036832598925173 1107 52-53 0.41634390313794256 1107 54-55 0.4460375696077179 1107 56-57 0.42963111625242334 1107 58-59 0.41907251503496923 1107 60-61 0.3655026455632324 1107 62-63 0.3979932559431276 1107 64-65 0.39839888119204403 1107 66-67 0.41827073041996954 1107 68-69 0.4233560231582061 1107 70-71 0.440979422645178 1107 72-73 0.41898036437270747 1107 74-75 0.3949484450419334 1107 76-77 0.3372321819357218 1107 78-79 0.4408450691396588 1107 80-81 0.4237163694673143 1107 82-83 0.4256046164895402 1107 84-85 0.43347408740330096 1107 86-87 0.4253785723555481 1107 88-89 0.44436161544773967 1107 90-91 0.48370702178233316 1107 92-93 0.4849188726905638 1107 94-95 0.45880098960236637 1107 96-97 0.42651449310048406 1107 98-99 0.4780730836612186 1107 100 0.41198359392415185 1108 1 1.37210614338899 1108 2 0.8561504509100963 1108 3 0.4417610240013943 1108 4 0.3719879641365651 1108 5 0.20327560952888746 1108 6 0.15363493405238415 1108 7 0.11892876677527653 1108 8 0.11883657284398197 1108 9 0.15969649949943943 1108 10-11 0.11631588204442522 1108 12-13 0.11970253534828856 1108 14-15 0.1448675856338042 1108 16-17 0.10640074813046851 1108 18-19 0.09595961485561588 1108 20-21 0.10826231326051072 1108 22-23 0.09641907914479475 1108 24-25 0.15251054480632575 1108 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2316 62-63 -2.3188714190840543 2316 64-65 -1.6785438528348493 2316 66-67 -1.3977059651532855 2316 68-69 -1.3332317825208193 2316 70-71 -1.2318057767354347 2316 72-73 -1.2027760960394929 2316 74-75 -1.3336371273272398 2316 76-77 -1.1494638951095268 2316 78-79 -1.4126626875629675 2316 80-81 -1.2923692640633782 2316 82-83 -1.2447297571499192 2316 84-85 -1.172782753371603 2316 86-87 -1.3024438873251256 2316 88-89 -1.261022073496509 2316 90-91 -1.2386274633402863 2316 92-93 -1.1766545289560888 2316 94-95 -1.1790266922272394 2316 96-97 -1.1452246549497183 2316 98-99 -1.2095092405292398 2316 100 -1.1033860285919062 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 74.0 7 1041.0 8 1354.0 9 675.0 10 580.0 11 893.0 12 1584.0 13 6360.0 14 29459.0 15 82985.0 16 135745.0 17 157832.0 18 168747.0 19 171969.0 20 183973.0 21 226584.0 22 312679.0 23 452746.0 24 620372.0 25 728930.0 26 707269.0 27 608946.0 28 555714.0 29 571821.0 30 647173.0 31 759670.0 32 914253.0 33 1143546.0 34 1521945.0 35 2213674.0 36 3677710.0 37 6898681.0 38 9258373.0 39 2849368.0 40 5682.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.269868693859056 37.698288821458334 26.724935575429342 18.306906909253268 2 20.174807368560867 36.245542929530785 26.054941199363572 17.524708502544765 3 26.994435770246543 27.37053625110185 25.91503320179367 19.719994776857934 4 19.23089654177965 28.02000381431994 25.46695027658031 27.28214936732011 5 19.60994133576047 35.970279196271306 25.325089366004878 19.094690101963348 6 27.851728461635016 27.422023674444507 25.597166655993348 19.129081207927126 7 19.336827725058 27.68234413178557 33.89530587372984 19.08552226942659 8 27.526312448504502 27.56078900440438 25.597514790596897 19.31538375649422 9 27.46823012045429 27.402427065309237 25.82659858988079 19.302744224355685 10-11 18.86542820401822 32.14312897261239 29.956794530423554 19.034648292945835 12-13 18.885114654341503 27.86932470056844 29.856947280095937 23.388613364994118 14-15 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33.68168084667123 19.45733731799596 40-41 19.54602287255789 27.33677960442887 33.694336650981356 19.42286087203189 42-43 19.586536915033836 27.28922813079909 33.620092154402634 19.504142799764452 44-45 19.62062490883768 27.24108661878161 33.53008319029914 19.60820528208158 46-47 19.65359023850637 27.236765742180957 33.443013557550486 19.666630461762182 48-49 19.691597451686523 27.176617981361385 33.37344245676495 19.75834211018714 50-51 19.705820925680364 27.209352737195687 33.27335077057208 19.811475566551866 52-53 19.844641263466283 27.341172325281782 32.99966905673449 19.81451735451745 54-55 20.304367503880755 27.57058759129431 32.1624811858066 19.962563719018338 56-57 20.833023565768688 28.084159111894337 30.91846073558548 20.1643565867515 58-59 21.112128250788125 28.6069172504403 30.08690367655752 20.194050822214056 60-61 21.24490844679699 29.0130305467082 29.48465460429252 20.25740640220228 62-63 21.264820960323043 29.3017107551381 29.07542964002836 20.358038644510497 64-65 21.240345785499184 29.487383789639853 28.76459829084881 20.50767213401215 66-67 21.166112347218814 29.433181406282987 28.61712057261126 20.78358567388694 68-69 21.06885287775593 29.305654254669456 28.579594538058455 21.04589832951616 70-71 20.984323909186458 29.15657715404643 28.61092015877813 21.248178777988983 72-73 20.911466421187555 29.04366858006434 28.608024669348048 21.436840329400056 74-75 20.871650361131795 29.041618458696238 28.425116550177904 21.661614629994062 76-77 20.825868547206213 29.062406203960055 28.24098575002797 21.870739498805765 78-79 20.856640574012378 29.106949634684966 27.806257009158575 22.230152782144085 80-81 20.83045092187892 29.158328274636304 27.43974142147604 22.57147938200874 82-83 20.79272882576802 29.210441821114557 27.07840535933523 22.91842399378219 84-85 20.74773369694451 29.11653329391829 26.82843689892227 23.30729611021493 86-87 20.701194536793675 28.96890807116894 26.69962016418257 23.630277227854815 88-89 20.75634888931332 28.844088325402637 26.606809355277456 23.792753430006584 90-91 20.69070113735702 28.669863513507632 26.673656434987926 23.965778914147425 92-93 20.666262770481687 28.539634241101147 26.793136074651873 24.000966913765293 94-95 20.656630143743033 28.44336994610057 26.94758900188469 23.9524109082717 96-97 20.6507876432136 28.291687700996004 27.087641978213394 23.969882677577004 98-99 20.64457693280094 28.14355536570754 27.21429888166077 23.997568819830743 100 20.652421602037823 28.088161281987105 27.266348176412002 23.993068939563074 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 63967.0 1 40880.5 2 14023.5 3 9579.0 4 8963.0 5 9109.0 6 9512.0 7 10368.5 8 11265.0 9 12199.0 10 13945.0 11 21419.5 12 138694.5 13 169448.5 14 87725.0 15 93122.0 16 106826.5 17 120717.0 18 135207.0 19 151013.0 20 171199.0 21 199240.5 22 234932.5 23 273268.0 24 311161.0 25 352952.0 26 398315.0 27 451043.0 28 524338.5 29 600584.5 30 664036.5 31 728594.0 32 797234.5 33 860278.0 34 914338.0 35 979943.0 36 1053120.5 37 1096057.5 38 1123501.0 39 1142977.0 40 1144277.5 41 1151806.5 42 1177931.0 43 1181479.0 44 1171321.0 45 1175263.0 46 1170315.5 47 1160608.0 48 1137566.5 49 1107142.0 50 1086736.5 51 1064070.0 52 1025853.5 53 968043.0 54 893863.0 55 812161.0 56 728673.0 57 646595.5 58 565211.5 59 478916.5 60 392856.0 61 319364.0 62 252102.5 63 187614.5 64 136919.0 65 99458.5 66 71493.5 67 53282.5 68 40471.5 69 30624.0 70 23846.5 71 19507.5 72 16436.5 73 13220.5 74 10639.5 75 9313.5 76 7304.5 77 4354.5 78 2566.0 79 1866.5 80 1337.0 81 950.5 82 643.5 83 411.5 84 272.5 85 194.0 86 134.0 87 79.5 88 44.5 89 36.0 90 29.5 91 24.5 92 22.0 93 13.5 94 7.0 95 5.5 96 5.0 97 3.0 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10788242158050472 2 0.0 3 0.0 4 0.0 5 0.009416479518581503 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.580350238571871E-5 20-21 0.011026602059996674 22-23 0.0 24-25 8.94200574438941E-4 26-27 0.004921612580820922 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.4037685626984946E-6 40-41 0.007619655758327428 42-43 0.03908793562833958 44-45 0.01521825498821438 46-47 0.004732103824856625 48-49 0.0016157376156659671 50-51 0.0 52-53 0.0014585155366437358 54-55 5.1939436819844306E-5 56-57 8.506837489952876E-4 58-59 1.6845222752381934E-5 60-61 2.8356124966509593E-4 62-63 2.8356124966509593E-4 64-65 7.018842813492473E-5 66-67 2.9900270385477935E-4 68-69 5.432584337643173E-4 70-71 4.2674564306034236E-4 72-73 0.0012381238723000723 74-75 0.001666273283923113 76-77 0.002522572107169195 78-79 0.004531364920390741 80-81 0.0042899167276065995 82-83 0.007430147002363132 84-85 0.00245238367903427 86-87 0.0029324725274771555 88-89 0.00572597196724716 90-91 0.005789141552568592 92-93 0.0055982290280415965 94-95 0.0026924281032557128 96-97 0.005690877753179698 98-99 0.007811972051417123 100 0.3019085047795652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.5618407E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.26434031771031 #Duplication Level Percentage of deduplicated Percentage of total 1 71.00088787612053 27.87803024427588 2 15.466566503378004 12.145690614702653 3 5.088340910867312 5.993710475304663 4 2.3357323301190767 3.668439564034956 5 1.3465612119505017 2.643591884232647 6 0.8996686580818083 2.119493781846112 7 0.6557216284398606 1.8022534020922112 8 0.4776987626426272 1.5005221428599385 9 0.35277519291750375 1.2466336707322896 >10 2.0389482996874686 14.74525116946504 >50 0.1801723475036464 4.978921708643535 >100 0.1496113254314482 10.627778567773275 >500 0.006340746977610848 1.5066752319001822 >1k 8.046894521043937E-4 0.7776755099524862 >5k 1.0594776977980714E-4 0.25434052180365396 >10k+ 6.3568660854763E-5 8.110991510380531 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2547565 7.152383316861981 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 179023 0.5026137187999452 No Hit AAGCAGTGGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA 49958 0.1402589397105828 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 47438 0.13318394615458237 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002316218128452516 2.807537125396989E-6 0.0 0.0 1.4037685626984946E-5 2 0.0023190256655779133 2.807537125396989E-6 0.0 0.0 1.6845222752381934E-5 3 0.002324640739828707 2.807537125396989E-6 0.0 5.615074250793978E-6 2.2460297003175914E-5 4 0.002330255814079501 8.422611376190967E-6 0.0 5.615074250793978E-6 3.0882908379366884E-5 5 0.002333063351204898 1.4037685626984946E-5 2.807537125396989E-6 5.615074250793978E-6 3.0882908379366884E-5 6 0.002335870888330295 2.2460297003175914E-5 2.807537125396989E-6 5.615074250793978E-6 3.369044550476387E-5 7 0.0023386784254556922 3.649798263016086E-5 2.807537125396989E-6 5.615074250793978E-6 3.369044550476387E-5 8 0.002347101036831883 4.492059400635183E-5 2.807537125396989E-6 5.615074250793978E-6 3.369044550476387E-5 9 0.002347101036831883 4.772813113174882E-5 2.807537125396989E-6 5.615074250793978E-6 3.930551975555785E-5 10-11 0.0023527161110826773 5.3343205382542795E-5 2.807537125396989E-6 5.615074250793978E-6 4.772813113174882E-5 12-13 0.0023681575652723602 5.6150742507939786E-5 2.807537125396989E-6 5.615074250793978E-6 5.053566825714581E-5 14-15 0.0024299233820310943 7.018842813492473E-5 2.807537125396989E-6 5.615074250793978E-6 5.474697394524129E-5 16-17 0.0025618776269247527 7.580350238571871E-5 2.807537125396989E-6 5.615074250793978E-6 6.036204819603527E-5 18-19 0.003246916685521618 8.984118801270365E-5 2.807537125396989E-6 5.615074250793978E-6 6.457335388413075E-5 20-21 0.0034378292100486134 9.966756795159312E-5 2.807537125396989E-6 7.018842813492473E-6 6.738089100952774E-5 22-23 0.003901072835739117 1.0809017932778408E-4 2.807537125396989E-6 8.422611376190967E-6 7.86110395111157E-5 24-25 0.005230441664614591 1.1932032782937204E-4 2.807537125396989E-6 1.1230148501587957E-5 8.282234519921118E-5 26-27 0.007594387924198856 1.3195424489365848E-4 2.807537125396989E-6 1.544145418968344E-5 8.703365088730666E-5 28-29 0.014489699104173862 1.4318439339524645E-4 2.807537125396989E-6 1.6845222752381934E-5 8.984118801270365E-5 30-31 0.031040130458389114 1.5441454189683441E-4 2.807537125396989E-6 1.6845222752381934E-5 9.545626226349764E-5 32-33 0.0651839932089046 1.6704845896112086E-4 2.807537125396989E-6 1.6845222752381934E-5 9.966756795159312E-5 34-35 0.12304592959477385 1.7687483890001032E-4 2.807537125396989E-6 2.6671602691271397E-5 1.0247510507699012E-4 36-37 0.21282254425359337 1.8389368171350278E-4 2.807537125396989E-6 2.947913981666839E-5 1.1230148501587957E-4 38-39 0.3375094792981618 1.9091252452699527E-4 2.807537125396989E-6 3.5094214067462364E-5 1.1651279070397506E-4 40-41 0.5013054064995102 2.063539787166787E-4 2.807537125396989E-6 4.2113056880954836E-5 1.2212786495476903E-4 42-43 0.705573385132019 2.105652844047742E-4 2.807537125396989E-6 4.772813113174882E-5 1.2493540208016605E-4 44-45 0.9519516131083572 2.1618035865556817E-4 2.807537125396989E-6 5.3343205382542795E-5 1.27742939205563E-4 46-47 1.2424699397701868 2.358331185333471E-4 2.807537125396989E-6 5.3343205382542795E-5 1.33358013456357E-4 48-49 1.5761934552547507 2.47063267034935E-4 2.807537125396989E-6 5.3343205382542795E-5 1.4458816195794496E-4 50-51 1.9532597288811933 2.596971840992215E-4 2.807537125396989E-6 5.3343205382542795E-5 1.530107733341359E-4 52-53 2.3693493647820913 2.821574811023974E-4 2.807537125396989E-6 5.3343205382542795E-5 1.572220790222314E-4 54-55 2.8263771032769656 2.9759893529208086E-4 2.807537125396989E-6 5.6150742507939786E-5 1.586258475849299E-4 56-57 3.319803156834049 3.256743065460508E-4 2.807537125396989E-6 5.755451107063828E-5 1.6564469039842238E-4 58-59 3.84724392643388 3.383082236103372E-4 2.807537125396989E-6 6.036204819603527E-5 1.7125976464921634E-4 60-61 4.4064786502102695 3.4673083498652817E-4 2.807537125396989E-6 6.176581675873377E-5 1.7687483890001032E-4 62-63 4.993369018440381 3.5234590923732215E-4 2.807537125396989E-6 6.176581675873377E-5 1.7687483890001032E-4 64-65 5.607288950345253 3.635760577389101E-4 2.807537125396989E-6 6.316958532143226E-5 1.8950875596429677E-4 66-67 6.243944317891589 3.705949005524026E-4 2.807537125396989E-6 6.597712244682924E-5 1.9372006165239227E-4 68-69 6.90731480495464 3.80421280491292E-4 2.807537125396989E-6 7.86110395111157E-5 1.9372006165239227E-4 70-71 7.591615481287526 3.88843891867483E-4 2.807537125396989E-6 7.86110395111157E-5 2.0354644159128172E-4 72-73 8.295752811179906 3.986702718063725E-4 2.807537125396989E-6 8.00148080738142E-5 2.105652844047742E-4 74-75 9.020307954816733 4.0568911461986496E-4 2.807537125396989E-6 8.14185766365127E-5 2.1337282153017118E-4 76-77 9.759539498776572 4.197268002468499E-4 2.807537125396989E-6 8.14185766365127E-5 2.1337282153017118E-4 78-79 10.51466170286616 4.3095694874843786E-4 2.807537125396989E-6 8.14185766365127E-5 2.1618035865556817E-4 80-81 11.287099392176636 4.492059400635183E-4 2.807537125396989E-6 8.14185766365127E-5 2.1898789578096516E-4 82-83 12.070650717197992 4.6885869994129725E-4 2.807537125396989E-6 8.14185766365127E-5 2.2741050715715613E-4 84-85 12.86569890674785 4.7587754275478966E-4 2.807537125396989E-6 8.562988232460817E-5 2.3302558140795012E-4 86-87 13.676504398414 4.8430015413098066E-4 2.807537125396989E-6 9.405249370079914E-5 2.4285196134683957E-4 88 14.292259055830318 4.913189969444731E-4 2.807537125396989E-6 9.545626226349764E-5 2.470632670349351E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 326050 0.0 87.66289 8 GTGGTAT 329020 0.0 87.2015 6 AGTGGTA 330010 0.0 86.986176 5 TGGTATC 329765 0.0 86.66248 7 GTATCAA 331780 0.0 85.99311 9 AAGCAGT 328850 0.0 85.64763 1 GCAGTGG 336630 0.0 85.33419 3 CAGTGGT 342605 0.0 83.544174 4 AGCAGTG 345380 0.0 83.05799 2 ATCAACG 325945 0.0 44.69116 10-11 CAACGCA 325870 0.0 44.650314 12-13 AGAGTAC 319140 0.0 44.18642 18-19 ACGCAGA 331385 0.0 43.978733 14-15 TCAACGC 324615 0.0 43.97098 12-13 GAGTACT 314900 0.0 43.67456 20-21 AACGCAG 328250 0.0 43.52457 14-15 TATCAAC 332060 0.0 42.92845 10-11 CGCAGAG 333880 0.0 42.843437 16-17 AGTACTT 329560 0.0 42.431316 20-21 GTACTTT 325700 0.0 42.177666 22-23 >>END_MODULE