##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660759_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36299292 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.9767066531215 33.0 31.0 34.0 27.0 34.0 2 31.761410773521423 34.0 31.0 34.0 28.0 34.0 3 32.25129333101042 34.0 31.0 34.0 30.0 34.0 4 35.781906434979504 37.0 35.0 37.0 35.0 37.0 5 35.83983756487592 37.0 35.0 37.0 35.0 37.0 6 35.870498934249184 37.0 35.0 37.0 35.0 37.0 7 35.84994274819465 37.0 35.0 37.0 35.0 37.0 8 35.87183873999526 37.0 35.0 37.0 35.0 37.0 9 37.58306409943202 39.0 37.0 39.0 35.0 39.0 10-11 37.50451196128013 39.0 37.0 39.0 34.5 39.0 12-13 37.57897791780622 39.0 37.0 39.0 35.0 39.0 14-15 38.89375531070964 40.0 38.0 41.0 35.0 41.0 16-17 38.90047983580506 40.0 38.0 41.0 35.0 41.0 18-19 38.880093708163784 40.0 38.0 41.0 35.0 41.0 20-21 38.79291020331746 40.0 38.0 41.0 34.5 41.0 22-23 38.67062923706611 40.0 38.0 41.0 34.5 41.0 24-25 38.62524373753626 40.0 38.0 41.0 34.0 41.0 26-27 38.56261584936698 40.0 38.0 41.0 34.0 41.0 28-29 38.4431383813216 40.0 38.0 41.0 34.0 41.0 30-31 38.32081178057137 40.0 38.0 41.0 34.0 41.0 32-33 38.206363653043155 40.0 38.0 41.0 33.5 41.0 34-35 38.08594353575822 40.0 38.0 41.0 33.0 41.0 36-37 37.874296694271614 40.0 37.5 41.0 33.0 41.0 38-39 37.39007526648178 40.0 35.5 41.0 32.5 41.0 40-41 37.21733962469571 39.0 35.0 41.0 32.0 41.0 42-43 37.027370134381684 39.0 35.0 41.0 31.0 41.0 44-45 36.90597603115785 39.0 35.0 41.0 31.0 41.0 46-47 36.74198378304459 39.0 35.0 41.0 31.0 41.0 48-49 36.88711420321917 39.0 35.0 41.0 31.0 41.0 50-51 36.77972581393598 39.0 35.0 41.0 31.0 41.0 52-53 36.46561300699749 39.0 35.0 41.0 30.0 41.0 54-55 35.94106251438733 39.0 35.0 41.0 27.5 41.0 56-57 35.54985086210497 39.0 35.0 41.0 25.5 41.0 58-59 35.30041320089659 39.0 35.0 41.0 25.0 41.0 60-61 35.06183536582476 38.0 34.5 40.5 25.0 41.0 62-63 34.70470764278268 37.5 34.0 40.0 23.0 41.0 64-65 34.407714370847785 37.0 34.0 40.0 23.0 41.0 66-67 34.08866196343443 36.5 34.0 39.5 23.0 41.0 68-69 33.767592643404726 36.0 33.5 39.0 22.0 41.0 70-71 33.420498160129405 35.5 33.0 39.0 22.5 40.5 72-73 33.08748915819075 35.0 33.0 37.5 22.0 40.0 74-75 32.730249986693956 35.0 33.0 37.0 21.5 39.5 76-77 30.676266881458737 33.5 30.0 35.5 19.0 38.0 78-79 31.940619020888892 35.0 32.0 36.0 20.0 39.0 80-81 31.970958248992844 35.0 32.0 36.0 20.0 37.5 82-83 31.7875023981184 35.0 32.0 35.0 20.0 37.0 84-85 31.582768694221365 35.0 32.0 35.0 19.5 37.0 86-87 31.403259642088884 35.0 32.0 35.0 19.0 36.0 88-89 31.20217835102679 35.0 32.0 35.0 18.5 36.0 90-91 31.054041453480693 35.0 32.0 35.0 18.0 36.0 92-93 30.919268728436904 35.0 32.0 35.0 17.5 35.0 94-95 30.831198209044956 35.0 31.5 35.0 17.0 35.0 96-97 30.712307680821986 35.0 31.0 35.0 16.5 35.0 98-99 30.55712670649334 35.0 31.0 35.0 15.0 35.0 100 30.360920510515744 35.0 31.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7541497887656092 1101 2 1.2178267748996987 1101 3 0.7834002299078264 1101 4 0.6170242458973334 1101 5 0.48341051633433807 1101 6 0.4405899053418594 1101 7 0.41095840990575283 1101 8 0.41454354686512573 1101 9 0.5126744766276019 1101 10-11 0.5078339763004891 1101 12-13 0.4869617303743681 1101 14-15 0.6263405679672545 1101 16-17 0.5937416647190048 1101 18-19 0.5362223073742172 1101 20-21 0.5947844919130034 1101 22-23 0.560961204740785 1101 24-25 0.6132778498632803 1101 26-27 0.5947720919897179 1101 28-29 0.6368895244716839 1101 30-31 0.5780898819951759 1101 32-33 0.607441702203694 1101 34-35 0.599840552499181 1101 36-37 0.5933759913422847 1101 38-39 0.6867971281685215 1101 40-41 0.6933502556047344 1101 42-43 0.6379029229595048 1101 44-45 0.623822706921608 1101 46-47 0.6156298729721712 1101 48-49 0.5933233709625156 1101 50-51 0.595964165877696 1101 52-53 0.5900707069868716 1101 54-55 0.628807707888626 1101 56-57 0.6591437012774577 1101 58-59 0.648655542133163 1101 60-61 0.59502444477517 1101 62-63 0.6325349862114606 1101 64-65 0.6212308322151259 1101 66-67 0.5369050287984365 1101 68-69 0.5756669666974759 1101 70-71 0.5896075733218495 1101 72-73 0.5143218659072062 1101 74-75 0.4848115628149543 1101 76-77 0.4273621180164575 1101 78-79 0.5345772024263944 1101 80-81 0.5289431693175395 1101 82-83 0.46236164822446213 1101 84-85 0.43457491572864626 1101 86-87 0.3980568075498816 1101 88-89 0.4349285879947047 1101 90-91 0.42130050394835195 1101 92-93 0.3461988518809207 1101 94-95 0.2621845266176628 1101 96-97 0.22774484477703183 1101 98-99 0.20196076943353702 1101 100 0.12135631628468602 1102 1 1.66613020255177 1102 2 1.1417091729733073 1102 3 0.7146990612531283 1102 4 0.5791970976584722 1102 5 0.42762287739078175 1102 6 0.40339910815717417 1102 7 0.3774262624422775 1102 8 0.38967033326755995 1102 9 0.4720693489969179 1102 10-11 0.46941875659700827 1102 12-13 0.39552440106660924 1102 14-15 0.5738981638476659 1102 16-17 0.5447356715400105 1102 18-19 0.5229711508827108 1102 20-21 0.5318573956899115 1102 22-23 0.5221019022122206 1102 24-25 0.5602472207705773 1102 26-27 0.5727608959886794 1102 28-29 0.5592168380527269 1102 30-31 0.5749673138159324 1102 32-33 0.5506348536096581 1102 34-35 0.5293069958127532 1102 36-37 0.573044977596112 1102 38-39 0.6325228567329688 1102 40-41 0.5713364576113094 1102 42-43 0.6075403331402143 1102 44-45 0.5933688737965568 1102 46-47 0.6347975895109883 1102 48-49 0.5718130591975026 1102 50-51 0.5461678096931735 1102 52-53 0.4635376309005679 1102 54-55 0.5313404886675173 1102 56-57 0.6150630063740294 1102 58-59 0.6157290024973676 1102 60-61 0.5452139765402961 1102 62-63 0.6195614015490492 1102 64-65 0.5864050462302899 1102 66-67 0.5235296279399151 1102 68-69 0.49681140630486453 1102 70-71 0.530068016812173 1102 72-73 0.4670537457168038 1102 74-75 0.5060781455426167 1102 76-77 0.3917726804381694 1102 78-79 0.5221568441989923 1102 80-81 0.519297759505644 1102 82-83 0.4890466055268199 1102 84-85 0.43152293206391334 1102 86-87 0.428985777009661 1102 88-89 0.4339990986963542 1102 90-91 0.4144935096845437 1102 92-93 0.32893316117482385 1102 94-95 0.27573686351898274 1102 96-97 0.25114975984221743 1102 98-99 0.24304745882614398 1102 100 0.15463566371160198 1103 1 1.6230358589640872 1103 2 1.0876338356970479 1103 3 0.6574820444273897 1103 4 0.5379116278388665 1103 5 0.4051007881270152 1103 6 0.36981104192881276 1103 7 0.3311821572559879 1103 8 0.37851972066093964 1103 9 0.44032973387242436 1103 10-11 0.39492999579098864 1103 12-13 0.38037569669178595 1103 14-15 0.5414440646987018 1103 16-17 0.4947411126295975 1103 18-19 0.4215753118237089 1103 20-21 0.41878392295297573 1103 22-23 0.47033043156755383 1103 24-25 0.529182787618538 1103 26-27 0.47081058865482817 1103 28-29 0.500679281455092 1103 30-31 0.5028356049951768 1103 32-33 0.4735775946491074 1103 34-35 0.4835406994300584 1103 36-37 0.5116456362217008 1103 38-39 0.5473349989946499 1103 40-41 0.5108332672559399 1103 42-43 0.470120547654318 1103 44-45 0.48293189518754076 1103 46-47 0.4798981625656822 1103 48-49 0.4747614865408636 1103 50-51 0.41783425029775145 1103 52-53 0.41592230637686356 1103 54-55 0.488203642853982 1103 56-57 0.49897397375846 1103 58-59 0.49557684055839957 1103 60-61 0.47850957193928423 1103 62-63 0.4880106792651375 1103 64-65 0.4565638176266802 1103 66-67 0.4339732805921628 1103 68-69 0.4185367092850072 1103 70-71 0.4701624966370943 1103 72-73 0.4085575513878652 1103 74-75 0.37209366680387035 1103 76-77 0.23608234204722223 1103 78-79 0.3664590960865759 1103 80-81 0.3984846941751279 1103 82-83 0.38771746543115526 1103 84-85 0.3780274976946387 1103 86-87 0.3411251626830136 1103 88-89 0.3666049026720657 1103 90-91 0.3490014300997508 1103 92-93 0.2954813295312064 1103 94-95 0.26634057084879714 1103 96-97 0.22595430783642811 1103 98-99 0.24111332829595256 1103 100 0.14789056020518743 1104 1 1.5843449493268977 1104 2 1.0773026019132672 1104 3 0.6418517532127197 1104 4 0.5278598033372219 1104 5 0.3887753580955362 1104 6 0.36630768436348404 1104 7 0.3057566120001667 1104 8 0.3298328189883506 1104 9 0.4045679412308374 1104 10-11 0.3852500637113252 1104 12-13 0.3602088274104034 1104 14-15 0.5047658295335324 1104 16-17 0.49177105978090196 1104 18-19 0.4349095075739342 1104 20-21 0.4320654129014798 1104 22-23 0.4343272675521135 1104 24-25 0.4690011028697967 1104 26-27 0.4506765621956248 1104 28-29 0.4915484249779283 1104 30-31 0.45406216855217707 1104 32-33 0.4618460717729178 1104 34-35 0.43238378168290836 1104 36-37 0.5008378748993039 1104 38-39 0.5587681097941086 1104 40-41 0.5418482577701056 1104 42-43 0.5272364048397691 1104 44-45 0.5272114197311382 1104 46-47 0.5540165238870642 1104 48-49 0.5464585333373577 1104 50-51 0.5318067655890957 1104 52-53 0.4930507680394882 1104 54-55 0.5263422052025959 1104 56-57 0.5968306375259758 1104 58-59 0.5579851463357599 1104 60-61 0.5424626787071674 1104 62-63 0.6000561590360576 1104 64-65 0.5929957417355496 1104 66-67 0.5641417515080605 1104 68-69 0.5658560504932595 1104 70-71 0.5558908950431416 1104 72-73 0.5071813517829042 1104 74-75 0.47159719523939003 1104 76-77 0.44444528159807817 1104 78-79 0.5100399020186792 1104 80-81 0.49158936072912596 1104 82-83 0.44595908840993204 1104 84-85 0.465299522831053 1104 86-87 0.482302816588561 1104 88-89 0.4850429213851264 1104 90-91 0.49944682176121447 1104 92-93 0.4726913191575406 1104 94-95 0.43757226936181937 1104 96-97 0.42546243072024126 1104 98-99 0.42581337411812115 1104 100 0.32528252369521127 1105 1 1.5823703367850683 1105 2 1.0563401113584163 1105 3 0.6154523458124288 1105 4 0.5054040636517456 1105 5 0.35041817602832026 1105 6 0.284312454300796 1105 7 0.24390239810777103 1105 8 0.2741972055650237 1105 9 0.3585522920612476 1105 10-11 0.3201590000302943 1105 12-13 0.2920084952512312 1105 14-15 0.4168801219366216 1105 16-17 0.3522696059414514 1105 18-19 0.3380427584803698 1105 20-21 0.33475821850961296 1105 22-23 0.306912836900743 1105 24-25 0.37066604929263747 1105 26-27 0.3312142658946371 1105 28-29 0.3659264521958292 1105 30-31 0.3632738789635894 1105 32-33 0.3715723945394487 1105 34-35 0.3865930948041907 1105 36-37 0.42153854034182814 1105 38-39 0.45883035867645816 1105 40-41 0.4298765166413716 1105 42-43 0.3854365661495507 1105 44-45 0.4257508225242148 1105 46-47 0.4468770578733583 1105 48-49 0.4291646429689422 1105 50-51 0.40729663177463493 1105 52-53 0.3792398395085854 1105 54-55 0.4647265612156488 1105 56-57 0.45909358478223083 1105 58-59 0.44477690651707036 1105 60-61 0.40272833935669894 1105 62-63 0.4027086880104278 1105 64-65 0.38146651642197327 1105 66-67 0.4067305174552516 1105 68-69 0.4475545456403509 1105 70-71 0.4447710509238547 1105 72-73 0.4138234459874752 1105 74-75 0.340790991234158 1105 76-77 0.3089913230016812 1105 78-79 0.42859508964466286 1105 80-81 0.38562095935676766 1105 82-83 0.3687623605378967 1105 84-85 0.3928534085084152 1105 86-87 0.36255672927791593 1105 88-89 0.3688078050509773 1105 90-91 0.3687204850853938 1105 92-93 0.33231264258490967 1105 94-95 0.3225633150115712 1105 96-97 0.29548857766431524 1105 98-99 0.30767847918319546 1105 100 0.2944094343741952 1106 1 1.4019373133881388 1106 2 0.8676717929202482 1106 3 0.46637719363047125 1106 4 0.4290553307779774 1106 5 0.24976202232897293 1106 6 0.2041498129586401 1106 7 0.1463174045154645 1106 8 0.18790279659663867 1106 9 0.23637241721260693 1106 10-11 0.20844204320323456 1106 12-13 0.1947283741131116 1106 14-15 0.3088178295158457 1106 16-17 0.23743137344879983 1106 18-19 0.20103590310475283 1106 20-21 0.19695284415388414 1106 22-23 0.18876699528714624 1106 24-25 0.24682556711803727 1106 26-27 0.2016835091102962 1106 28-29 0.25918659544199585 1106 30-31 0.2473353097903086 1106 32-33 0.2263191921860681 1106 34-35 0.2447887136793767 1106 36-37 0.25224583781619714 1106 38-39 0.3309403167462506 1106 40-41 0.3405074923194533 1106 42-43 0.24965167959065582 1106 44-45 0.2395112219054667 1106 46-47 0.289997856336889 1106 48-49 0.2693837788731628 1106 50-51 0.2674057442150257 1106 52-53 0.21265559433961556 1106 54-55 0.2847280254695974 1106 56-57 0.2940554606671384 1106 58-59 0.20879385914553694 1106 60-61 0.2185936012497791 1106 62-63 0.2552690598464622 1106 64-65 0.27912203267433 1106 66-67 0.24891409279973686 1106 68-69 0.251684284061362 1106 70-71 0.26401700246527326 1106 72-73 0.24743956818008428 1106 74-75 0.20099018885247943 1106 76-77 0.13970535389761807 1106 78-79 0.26284611573445105 1106 80-81 0.25389373764489775 1106 82-83 0.253798345050793 1106 84-85 0.2818444610914952 1106 86-87 0.25722919276042333 1106 88-89 0.2637467432845675 1106 90-91 0.2725544806955469 1106 92-93 0.24898524748122242 1106 94-95 0.22717538056808095 1106 96-97 0.27353759837539826 1106 98-99 0.2781386683639333 1106 100 0.22937046270618922 1107 1 1.5665238030496766 1107 2 1.0418223994475042 1107 3 0.6110847383195122 1107 4 0.5152274042471348 1107 5 0.3534383641037948 1107 6 0.33065319031516793 1107 7 0.2634288421386941 1107 8 0.27817560422272436 1107 9 0.3540430021602248 1107 10-11 0.3310477647364536 1107 12-13 0.3143940348964662 1107 14-15 0.435252911926419 1107 16-17 0.4011949404727062 1107 18-19 0.3578047708664087 1107 20-21 0.37672940313650116 1107 22-23 0.3844594220508739 1107 24-25 0.4331672533920141 1107 26-27 0.4033525203082462 1107 28-29 0.36813951837415715 1107 30-31 0.41875889610961536 1107 32-33 0.4166197044837219 1107 34-35 0.4416783654035399 1107 36-37 0.47972688381481277 1107 38-39 0.49078467425102446 1107 40-41 0.4678889588286239 1107 42-43 0.45167824869334083 1107 44-45 0.42960117146126464 1107 46-47 0.47910405262255296 1107 48-49 0.4438252634901758 1107 50-51 0.42104594019253483 1107 52-53 0.3942611379939578 1107 54-55 0.42132232641984046 1107 56-57 0.3690041285803147 1107 58-59 0.38216679462670555 1107 60-61 0.39030608453576576 1107 62-63 0.4520581475140162 1107 64-65 0.3832765284280484 1107 66-67 0.4009220518554386 1107 68-69 0.3367852814481509 1107 70-71 0.3709725002125879 1107 72-73 0.3828039148697826 1107 74-75 0.3726917433833634 1107 76-77 0.2993293603264142 1107 78-79 0.40089841976973517 1107 80-81 0.3588533948079764 1107 82-83 0.3689151238384589 1107 84-85 0.3669824058094875 1107 86-87 0.31107412516842814 1107 88-89 0.35951736778002896 1107 90-91 0.3855099290343027 1107 92-93 0.40392419865025175 1107 94-95 0.35952457483165645 1107 96-97 0.3249388912257203 1107 98-99 0.3750427908872389 1107 100 0.2756897250794914 1108 1 1.389901086925974 1108 2 0.8714558019304306 1108 3 0.45818218868448213 1108 4 0.3939554824746736 1108 5 0.21759994028690244 1108 6 0.16598903272414844 1108 7 0.12570633096952122 1108 8 0.1316098503588421 1108 9 0.15933605617475166 1108 10-11 0.1252131794885969 1108 12-13 0.1327441718948421 1108 14-15 0.17016306945470916 1108 16-17 0.13267092333066444 1108 18-19 0.09379467220313842 1108 20-21 0.11946672590823226 1108 22-23 0.08892897730705585 1108 24-25 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2316 56-57 -1.3712906163744378 2316 58-59 -1.1697824421626777 2316 60-61 -1.1542453281883525 2316 62-63 -2.175043581642548 2316 64-65 -1.5684603847942853 2316 66-67 -1.2744081467690265 2316 68-69 -1.1698216386935556 2316 70-71 -1.071400458082529 2316 72-73 -1.0099826737587279 2316 74-75 -1.1477723638413586 2316 76-77 -0.9774939386272514 2316 78-79 -1.2509710134667564 2316 80-81 -1.1151130039166794 2316 82-83 -1.0547325909918648 2316 84-85 -0.9663301095879717 2316 86-87 -1.1239582444789313 2316 88-89 -1.0871339688148893 2316 90-91 -1.0326568029624958 2316 92-93 -0.9777965154116259 2316 94-95 -0.9570031653083397 2316 96-97 -0.8752579204490836 2316 98-99 -0.8910308016393351 2316 100 -0.8440483330360138 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 78.0 7 957.0 8 1255.0 9 544.0 10 282.0 11 255.0 12 583.0 13 3821.0 14 14205.0 15 31725.0 16 52186.0 17 71929.0 18 86618.0 19 104221.0 20 129400.0 21 174181.0 22 261321.0 23 435024.0 24 664694.0 25 716202.0 26 676777.0 27 592304.0 28 559390.0 29 601958.0 30 702585.0 31 843186.0 32 1029889.0 33 1305579.0 34 1752148.0 35 2619180.0 36 4598574.0 37 7855843.0 38 8417224.0 39 1990370.0 40 4804.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.293840914597176 34.70981721487129 26.941085900061317 20.055255970470213 2 22.745383022897524 33.95354377710728 24.498386910686854 18.802686289308344 3 27.658016029623937 25.599510315518 25.222654480423472 21.519819174434588 4 21.565919247130218 26.48594082771642 24.53503225352164 27.41310767163172 5 21.79953474668204 32.79111269953548 24.720975571748742 20.68837698203374 6 28.68450436994749 25.746309321955923 25.047449961282993 20.521736346813597 7 21.222559933124867 25.871003214057176 32.043702119589554 20.862734733228404 8 28.55344947223764 26.357985164008156 24.733912165559595 20.354653198194608 9 28.474839123584005 25.24622518808356 24.940326659814744 21.33860902851769 10-11 20.847669701106017 30.046424872418996 28.617530611891823 20.48837481458316 12-13 20.90016383790626 25.91458946361819 28.685865278033525 24.499381420442027 14-15 20.79738772866424 33.46895168093086 24.730609070832564 21.003051519572338 16-17 24.774757314825866 25.941278689402537 24.83461109930188 24.449352896469716 18-19 21.08175483467156 29.54064766058592 24.802699834652277 24.574897670090245 20-21 24.51078927297733 29.840223287787254 24.887663456654924 20.761323982580492 22-23 24.926612893717046 25.70523000834286 28.527804343952496 20.8403527539876 24-25 20.947724791940352 30.05031327889615 24.728525324936253 24.27343660422724 26-27 21.31322592695232 25.82081682770681 31.949666686144607 20.91629055919626 28-29 21.340277105129214 25.989354007235182 32.03048836324411 20.639880524391497 30-31 21.24891995138638 26.105244697334594 32.04267317390102 20.603162177378003 32-33 21.173469443977034 25.862587071946198 31.95779273050284 21.006150753573927 34-35 21.029344043404482 26.308784479873605 31.95849246866854 20.70337900805338 36-37 21.522228312331823 25.854128229277862 31.666065277526624 20.957578180863692 38-39 21.17406449690534 26.201354009879864 31.74237117352041 20.882210319694387 40-41 21.25912846655537 26.0185672374691 31.775563026223214 20.94674126975232 42-43 21.382193345763092 26.212875473910145 31.793281439395688 20.61164974093107 44-45 21.494118357111734 26.376202049498758 31.408440457285124 20.721239136104376 46-47 21.16389351523891 26.24497165962858 31.48699747004705 21.104137355085456 48-49 21.704354745015642 26.380070304527514 31.224529017023162 20.69104593343368 50-51 21.815614199858224 26.330800060783556 30.99782221647739 20.85576352288083 52-53 21.641034008899226 26.480928276966676 31.07919320205224 20.79884451208186 54-55 22.238252846527434 26.66945460132844 29.997241680256813 21.09505087188731 56-57 22.344980114579606 27.541523508279415 28.90682172787559 21.206674649265395 58-59 22.503914505748153 27.60209103306656 28.306385945782814 21.587608515402472 60-61 22.37270509479352 28.3849269396121 27.47432659265389 21.768041372940488 62-63 22.56720658125835 28.413156676354117 27.102321930087818 21.91731481229971 64-65 22.302698288645885 28.498574089664125 26.879841678345734 22.318885943344256 66-67 22.149172524798733 28.540485530742433 26.949048951774664 22.361292992684177 68-69 22.415268346758047 27.770794132978015 27.30861872404154 22.505318796222397 70-71 21.863764926062625 28.341919741470406 27.545654155784575 22.24866117668239 72-73 21.999654093467438 27.702361037987377 27.789120049429698 22.508864819115484 74-75 21.975588276775955 27.722146487116824 27.782223074227808 22.520042161879417 76-77 21.92275904476125 27.855327348122614 27.72144251145231 22.500471095663826 78-79 21.96050160761765 27.695724802273382 27.33432266263926 23.009450927469707 80-81 21.818336448356618 28.120399422013747 27.111145377086604 22.95011875254303 82-83 21.98559664248502 27.942787158353223 26.80616437241867 23.265451826743085 84-85 21.611704602713566 28.28694924868527 26.668679612886198 23.432666535714965 86-87 21.59213125575182 28.12201785235367 26.383394867444878 23.902456024449634 88-89 21.67240594032456 28.08066643899118 26.279068434777308 23.967859185906953 90-91 21.6330772292669 27.989949038527023 26.057438993130717 24.319534739075362 92-93 21.27236173835596 27.665190647606234 26.415658158333038 24.646789455704766 94-95 21.591664697797714 27.64253986739227 26.494494127774892 24.27130130703512 96-97 21.65770562093494 27.38397625233113 26.53252147839471 24.42579664833922 98-99 21.495969798477248 27.23000195501224 26.857465501858474 24.41656274465204 100 21.655942223041478 27.443201472934547 26.701290716040948 24.199565587983024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 37918.0 1 24439.5 2 9023.0 3 6822.5 4 6520.0 5 6485.5 6 6755.0 7 7115.0 8 7413.0 9 7899.0 10 8712.0 11 11917.5 12 116287.0 13 145279.5 14 71228.5 15 75239.0 16 86509.5 17 97907.5 18 108672.0 19 119821.5 20 133718.0 21 153520.5 22 178429.0 23 206008.5 24 237521.0 25 273444.0 26 309061.0 27 347506.5 28 392313.5 29 436929.5 30 478548.5 31 518617.5 32 559165.0 33 600298.0 34 636933.5 35 677068.5 36 727161.5 37 771705.0 38 819069.5 39 860096.0 40 885636.5 41 922531.5 42 963436.0 43 980825.0 44 991792.0 45 1025937.0 46 1112709.0 47 1658324.0 48 2362473.5 49 2467480.0 50 2015155.0 51 1466151.5 52 1163259.5 53 1032681.0 54 963561.5 55 910861.5 56 851026.0 57 774932.0 58 687154.0 59 588582.0 60 486468.5 61 396549.5 62 317241.0 63 243657.0 64 184764.0 65 139224.0 66 102340.0 67 76491.5 68 58955.0 69 45751.5 70 36239.5 71 29461.5 72 24188.5 73 18904.5 74 14468.5 75 11686.5 76 9068.5 77 6113.0 78 4009.5 79 2822.5 80 1957.5 81 1411.0 82 963.5 83 623.0 84 425.0 85 297.5 86 195.5 87 105.0 88 71.5 89 64.5 90 43.5 91 26.5 92 19.5 93 15.0 94 14.0 95 8.5 96 6.5 97 5.0 98 2.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1073216524443507 2 0.0 3 0.0 4 0.0 5 0.009272908132753664 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.815598937852562E-5 20-21 0.010716462458827021 22-23 0.0 24-25 8.980891417937298E-4 26-27 0.005016626770571723 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.007355515363770731 42-43 0.03874593476919605 44-45 0.014858416522283684 46-47 0.004900922034512409 48-49 0.0017465905395620388 50-51 0.0 52-53 0.0014146281420585282 54-55 5.372005602753905E-5 56-57 9.25637888474519E-4 58-59 2.6171309346749796E-5 60-61 2.961490268184845E-4 62-63 2.58958218799419E-4 64-65 6.887186670197314E-5 66-67 2.961490268184845E-4 68-69 5.372005602753905E-4 70-71 4.3940250955858866E-4 72-73 0.001292036219329016 74-75 0.0017231741048833681 76-77 0.002567543190649559 78-79 0.00451110726897924 80-81 0.004250771612845782 82-83 0.007398215921125955 84-85 0.0025399944439687696 86-87 0.002746610044074689 88-89 0.005553827330847114 90-91 0.005697080813587219 92-93 0.005500107274819575 94-95 0.002633660182683453 96-97 0.005518013960162088 98-99 0.007834863556016465 100 0.30090118562092066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.6299292E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.50486412975644 #Duplication Level Percentage of deduplicated Percentage of total 1 52.39935030688454 14.93636360985255 2 20.472539437471664 11.671339101124202 3 10.923425451290877 9.341122749617096 4 5.716299793513021 6.517693957561738 5 3.042649294649845 4.3365152369246545 6 1.7210905850738856 2.9435671969520474 7 1.1462315971064556 2.287122315572727 8 0.7855961633502946 1.7914649517726473 9 0.5674814485631335 1.455838342870454 >10 2.7970339261979493 14.048916504794295 >50 0.2789348893710567 5.557741574267425 >100 0.14249242009821675 6.630243972926027 >500 0.0035357632106333674 0.6854438264901415 >1k 0.002712585408159403 1.6913133812650971 >5k 1.349035293592194E-4 0.23235321061376704 >10k+ 4.914342808873912E-4 15.872960067395278 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2237755 6.164734562866956 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 146907 0.4047103728634707 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 133584 0.3680071776606552 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 126934 0.34968726111793036 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 122774 0.338226982498722 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 111657 0.3076010408136886 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 107063 0.294945146588534 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 99540 0.27422022446057626 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 87184 0.24018099306179302 No Hit AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA 86717 0.2388944665918002 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 85079 0.2343819818854869 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 83359 0.22964359745639118 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 82766 0.22800995677822034 No Hit TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG 81888 0.22559117681964705 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 80495 0.22175363640701312 No Hit TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT 78449 0.21611716283612362 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 77993 0.21486093998747965 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77372 0.21315016281860263 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 74994 0.20659907085791096 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 73550 0.20262103183720498 No Hit TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG 73168 0.2015686697139988 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 70764 0.1949459510119371 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 69477 0.19140042731411952 No Hit TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG 67162 0.1850228924575168 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 67155 0.18500360833484025 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 66011 0.18185203171455797 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 65188 0.179584769862729 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 65108 0.1793643798892827 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 64319 0.1771907837761684 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 62101 0.17108047176236935 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 60683 0.16717405948303343 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 59761 0.16463406503906466 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 59728 0.16454315417501808 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 58201 0.16033646055686157 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 56892 0.15673032961634623 No Hit CTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA 56550 0.15578816247986324 No Hit TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT 56478 0.15558981150376155 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 56327 0.15517382542888164 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 56225 0.1548928282127376 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 56219 0.15487629896472913 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 55131 0.15187899532585925 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 53667 0.14784585881179169 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 53143 0.14640230448571834 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 47741 0.13152047152875598 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 47264 0.13020639631208233 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 44647 0.1229968893057198 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 44466 0.12249825699079751 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 42049 0.11583972491805074 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 40017 0.11024181959251438 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.03224029824052767 1.1019498672315703E-5 0.0 2.7548746680789258E-6 4.407799468926281E-5 2 0.03224305311519574 1.6529248008473554E-5 0.0 2.7548746680789258E-6 4.407799468926281E-5 3 0.032248562864531906 1.6529248008473554E-5 0.0 5.5097493361578516E-6 4.407799468926281E-5 4 0.03227060186187653 2.2038997344631406E-5 0.0 1.1019498672315703E-5 4.407799468926281E-5 5 0.032284376235216926 3.030362134886818E-5 0.0 1.3774373340394628E-5 4.958774402542066E-5 6 0.032292640859221164 3.030362134886818E-5 0.0 1.3774373340394628E-5 5.2342618693499584E-5 7 0.032295395733889246 4.1323120021183886E-5 0.0 1.3774373340394628E-5 5.2342618693499584E-5 8 0.03229815060855733 4.1323120021183886E-5 0.0 1.3774373340394628E-5 5.2342618693499584E-5 9 0.03232018960590195 4.1323120021183886E-5 0.0 1.3774373340394628E-5 5.509749336157851E-5 10-11 0.03233396397924235 5.096518135946012E-5 0.0 1.3774373340394628E-5 6.336211736581529E-5 12-13 0.03236289016325718 5.785236802965744E-5 0.0 1.3774373340394628E-5 6.611699203389422E-5 14-15 0.03250063389666112 6.060724269773636E-5 0.0 1.515181067443409E-5 7.02493040360126E-5 16-17 0.03257914782470137 6.473955469985475E-5 0.0 1.6529248008473554E-5 7.4381616038131E-5 18-19 0.03265490687807354 7.4381616038131E-5 0.0 1.928412267655248E-5 7.4381616038131E-5 20-21 0.03280917985948596 8.815598937852562E-5 0.0 2.2038997344631406E-5 7.713649070620992E-5 22-23 0.033378061478444264 9.228830138064401E-5 0.0 2.3416434678670866E-5 7.989136537428885E-5 24-25 0.03565909770361361 1.033078000529597E-4 0.0 2.6171309346749792E-5 8.126880270832831E-5 26-27 0.042560058747151325 1.184596107273938E-4 1.3774373340394629E-6 3.856824535310496E-5 8.815598937852562E-5 28-29 0.059636149377238545 1.2396936006355165E-4 2.7548746680789258E-6 3.856824535310496E-5 8.815598937852562E-5 30-31 0.09504730836072506 1.3636629606990683E-4 2.7548746680789258E-6 3.856824535310496E-5 8.815598937852562E-5 32-33 0.15961881570582698 1.4600835740818306E-4 2.7548746680789258E-6 3.856824535310496E-5 9.091086404660455E-5 34-35 0.2715204472858589 1.5840529341453824E-4 2.7548746680789258E-6 4.1323120021183886E-5 9.091086404660455E-5 36-37 0.4461202714366991 1.6253760541665662E-4 2.7548746680789258E-6 5.096518135946012E-5 9.228830138064401E-5 38-39 0.6875120319151129 1.6253760541665662E-4 2.7548746680789258E-6 6.198468003177583E-5 9.504317604872294E-5 40-41 0.9846776074861185 1.6529248008473555E-4 2.7548746680789258E-6 6.749442936793368E-5 1.0468523738699917E-4 42-43 1.3272063267790457 1.6529248008473555E-4 2.7548746680789258E-6 7.162674137005207E-5 1.0468523738699917E-4 44-45 1.7201244586258047 1.8733147742936693E-4 2.7548746680789258E-6 7.575905337217046E-5 1.184596107273938E-4 46-47 2.1597762843418544 1.9835097610168263E-4 2.7548746680789258E-6 8.126880270832831E-5 1.294791093997095E-4 48-49 2.645873919524381 2.0799303743995886E-4 2.7548746680789258E-6 8.815598937852562E-5 1.3361142140182788E-4 50-51 3.177725890631696 2.2176741078035352E-4 2.7548746680789258E-6 8.815598937852562E-5 1.4187604540606469E-4 52-53 3.7389351836393945 2.479387201271033E-4 2.7548746680789258E-6 9.091086404660455E-5 1.5427298141241984E-4 54-55 4.33209826792214 2.699777174717347E-4 2.7548746680789258E-6 9.366573871468348E-5 1.5702785608049877E-4 56-57 4.957683472173507 2.7548746680789257E-4 2.7548746680789258E-6 9.366573871468348E-5 1.597827307485777E-4 58-59 5.613809216995197 2.9063927748232665E-4 2.7548746680789258E-6 9.366573871468348E-5 1.6253760541665662E-4 60-61 6.293604844965021 3.0165877615464235E-4 8.264624004236777E-6 9.504317604872294E-5 1.9008635209744588E-4 62-63 6.983943378289582 3.181880241631159E-4 8.264624004236777E-6 9.642061338276239E-5 1.9284122676552478E-4 64-65 7.688983300280347 3.305849601694711E-4 8.264624004236777E-6 1.0468523738699917E-4 1.9284122676552478E-4 66-67 8.411278379754624 3.402270215077473E-4 8.264624004236777E-6 1.074401120550781E-4 1.9284122676552478E-4 68-69 9.154283229546184 3.4711420817794465E-4 8.264624004236777E-6 1.074401120550781E-4 2.0386072543784048E-4 70-71 9.915966129587321 3.663983308544971E-4 8.264624004236777E-6 1.074401120550781E-4 2.066156001059194E-4 72-73 10.681643597897171 3.705306428566155E-4 8.264624004236777E-6 1.1294986139123595E-4 2.066156001059194E-4 74-75 11.44868472916772 3.774178295268128E-4 8.264624004236777E-6 1.2672423473163058E-4 2.0937047477399834E-4 76-77 12.225883083339475 3.870598908650891E-4 8.264624004236777E-6 1.3085654673374898E-4 2.1074791210803781E-4 78-79 13.01087222307256 4.022117015395231E-4 8.264624004236777E-6 1.3498885873586736E-4 2.1212534944207726E-4 80-81 13.809237656756501 4.0772145087568097E-4 8.264624004236777E-6 1.3498885873586736E-4 2.2314484811439297E-4 82-83 14.601531346671996 4.132312002118388E-4 8.264624004236777E-6 1.3498885873586736E-4 2.2452228544843242E-4 84-85 15.391177602031467 4.132312002118388E-4 8.264624004236777E-6 1.3774373340394628E-4 2.3278690945266922E-4 86-87 16.188682137381633 4.2838301088627296E-4 8.264624004236777E-6 1.3774373340394628E-4 2.3967409612286655E-4 88 16.787952778803508 4.3251532288839133E-4 8.264624004236777E-6 1.404986080720252E-4 2.451838454590244E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 286180 0.0 41.760593 20-21 AGTACTT 294755 0.0 41.33266 20-21 AGAGTAC 298035 0.0 41.272495 18-19 GTACTTT 294400 0.0 40.587616 22-23 TACTTTT 308355 0.0 39.503723 22-23 ACTTTTT 331770 0.0 36.138412 24-25 CTTTTTT 441655 0.0 27.82813 24-25 TAGCAGT 22190 0.0 23.449697 1 TCCGATC 4755 0.0 20.259747 6 GTGGTAT 1387965 0.0 19.003078 6 TGGTATC 1384110 0.0 18.967733 7 GGTATCA 1395165 0.0 18.809351 8 CAGTGGT 1401590 0.0 18.797413 4 AGTGGTA 1405485 0.0 18.795809 5 GTATCAA 1395540 0.0 18.754799 9 GCAGTGG 1410330 0.0 18.749567 3 AGCAGTG 1422370 0.0 18.570042 2 AAGCAGT 1401580 0.0 18.461563 1 CCGATCT 5620 0.0 17.55956 7 CGATCTA 2025 0.0 16.476463 8 >>END_MODULE