FastQCFastQC Report
Thu 26 May 2016
SRR1660758_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660758_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30861772
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13456654.36029726355311No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1046600.33912505088819916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1667850.087.106668
GTGGTAT1699650.085.427176
GTATCAA1706200.085.090969
TGGTATC1702850.085.0873267
AGTGGTA1714600.084.715875
GCAGTGG1806750.081.075383
AAGCAGT1816800.080.246231
CAGTGGT1828300.079.46294
AGCAGTG1907650.076.796432
CAACGCA1637550.045.34595512-13
ATCAACG1658250.044.8294710-11
TCAACGC1633650.044.72388512-13
ACGCAGA1670950.044.43401714-15
AACGCAG1654350.044.23652314-15
AGAGTAC1674750.043.69235618-19
CGCAGAG1684000.043.3466716-17
GAGTACT1650050.043.19790320-21
TATCAAC1705250.042.580210-11
AGTACTT1750950.041.43509720-21
GTACTTT1752450.040.68044322-23
Produced by FastQC (version 0.11.5)