##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660758_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30861772 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.641150125793164 33.0 31.0 34.0 29.0 34.0 2 31.79378682468395 34.0 31.0 34.0 30.0 34.0 3 31.834482835269473 34.0 31.0 34.0 30.0 34.0 4 35.24352188850336 37.0 35.0 37.0 32.0 37.0 5 35.237483641574435 37.0 35.0 37.0 32.0 37.0 6 35.243707555094375 37.0 35.0 37.0 32.0 37.0 7 35.23640628930834 37.0 35.0 37.0 32.0 37.0 8 35.231054360715255 37.0 35.0 37.0 32.0 37.0 9 36.83983278730722 39.0 37.0 39.0 33.0 39.0 10-11 36.79496933941447 39.0 37.0 39.0 32.0 39.0 12-13 36.80735845304022 39.0 37.0 39.0 32.5 39.0 14-15 38.08906091004755 40.0 38.0 41.0 33.0 41.0 16-17 38.0572321803168 40.0 38.0 41.0 32.5 41.0 18-19 38.01322036855175 40.0 38.0 41.0 32.0 41.0 20-21 37.98018759259838 40.0 38.0 41.0 32.0 41.0 22-23 37.867037090417234 40.0 38.0 41.0 32.0 41.0 24-25 37.79050234056554 40.0 38.0 41.0 32.0 41.0 26-27 37.753262158763924 40.0 38.0 41.0 32.0 41.0 28-29 37.664651854728234 40.0 38.0 41.0 32.0 41.0 30-31 37.549268655085655 40.0 37.0 41.0 31.0 41.0 32-33 37.426566028029754 40.0 37.0 41.0 31.0 41.0 34-35 37.29917034575979 40.0 37.0 41.0 30.5 41.0 36-37 37.1111888844231 40.0 37.0 41.0 30.0 41.0 38-39 36.71788674350909 39.0 35.0 41.0 30.0 41.0 40-41 36.70606906499083 39.0 35.0 41.0 30.0 41.0 42-43 36.73567869012836 40.0 35.0 41.0 30.0 41.0 44-45 36.68330386213728 39.5 35.0 41.0 30.0 41.0 46-47 36.807129172621714 40.0 35.0 41.0 30.0 41.0 48-49 36.757338285695326 40.0 35.0 41.0 30.0 41.0 50-51 35.663329782230264 38.5 34.0 40.0 28.0 40.5 52-53 35.72821197694027 38.5 35.0 40.0 28.0 40.5 54-55 36.05909751390814 39.0 35.0 41.0 27.5 41.0 56-57 35.78674693403865 39.0 35.0 41.0 26.0 41.0 58-59 35.51938185856599 39.0 35.0 41.0 25.0 41.0 60-61 35.31068211183726 39.0 35.0 41.0 25.0 41.0 62-63 35.072368479036136 38.5 34.0 41.0 24.0 41.0 64-65 34.81328037482747 38.0 34.0 40.0 24.0 41.0 66-67 34.54416457357018 37.0 34.0 40.0 23.0 41.0 68-69 34.261959131834686 37.0 34.0 40.0 23.0 41.0 70-71 33.95663894477608 36.0 33.0 39.0 23.5 41.0 72-73 33.62708670778852 36.0 33.0 39.0 23.0 41.0 74-75 33.280166236080035 35.0 33.0 38.5 22.5 40.5 76-77 32.90502209335226 35.0 33.0 37.0 21.5 39.5 78-79 32.552694657325574 35.0 32.0 37.0 21.0 39.0 80-81 32.223252200165305 35.0 32.0 36.5 20.0 39.0 82-83 31.912459093405268 35.0 32.0 36.0 20.0 38.0 84-85 31.641848125246987 35.0 32.0 36.0 20.0 37.0 86-87 31.387002081409975 35.0 31.0 35.0 19.0 37.0 88-89 31.193788564700693 35.0 31.0 35.0 19.0 36.0 90-91 31.021869110432156 35.0 31.0 35.0 18.5 36.0 92-93 30.858005172224072 35.0 31.0 35.0 18.0 36.0 94-95 30.698611392113193 35.0 31.0 35.0 18.0 35.5 96-97 30.58863836464089 34.0 31.0 35.0 17.0 35.0 98-99 30.494048802512054 34.0 30.5 35.0 17.0 35.0 100 30.382217456599705 34.0 30.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6939815113508594 1101 2 0.6322122622575357 1101 3 0.6338673423353356 1101 4 0.5364011700741713 1101 5 0.5516616902316755 1101 6 0.5718540777486396 1101 7 0.5086435662814495 1101 8 0.5777804125746826 1101 9 0.7192469155969903 1101 10-11 0.6846938089614341 1101 12-13 0.6668605392234923 1101 14-15 0.8343967246531463 1101 16-17 0.8343907725234345 1101 18-19 0.8004154296801573 1101 20-21 0.7351148172715938 1101 22-23 0.7280996179032257 1101 24-25 0.6892825231220314 1101 26-27 0.6941182615720791 1101 28-29 0.6762428713751305 1101 30-31 0.7264305780417004 1101 32-33 0.7434722446822519 1101 34-35 0.6789460785767076 1101 36-37 0.7484653769616187 1101 38-39 0.7890014909042478 1101 40-41 0.7494441734228303 1101 42-43 0.6838574944245792 1101 44-45 0.6250994516040365 1101 46-47 0.7335121626206913 1101 48-49 0.7419877890530131 1101 50-51 0.7116910199299795 1101 52-53 0.7408302922919887 1101 54-55 0.8297562022215743 1101 56-57 0.7766143627774724 1101 58-59 0.7691076713421197 1101 60-61 0.6939525108053459 1101 62-63 0.690706721612969 1101 64-65 0.6322033821435795 1101 66-67 0.6148607990514634 1101 68-69 0.6437905180062486 1101 70-71 0.6238683287293654 1101 72-73 0.5597642209426894 1101 74-75 0.5088179064164535 1101 76-77 0.4869270897405116 1101 78-79 0.4492597542943102 1101 80-81 0.46581340316013353 1101 82-83 0.4482737861982109 1101 84-85 0.37259364385450766 1101 86-87 0.35924094774450666 1101 88-89 0.34863881269440355 1101 90-91 0.34183766409096705 1101 92-93 0.3027378643415055 1101 94-95 0.2967497604225571 1101 96-97 0.28637957372353284 1101 98-99 0.2862604237951416 1101 100 0.24500263220747698 1102 1 0.5855578022069814 1102 2 0.5912362514660394 1102 3 0.5684954376992408 1102 4 0.524912690565543 1102 5 0.49897312478530154 1102 6 0.5381853867521613 1102 7 0.5196735910887327 1102 8 0.5677252523220773 1102 9 0.6666648323778972 1102 10-11 0.6145560566130541 1102 12-13 0.6003019571044348 1102 14-15 0.7395583042672698 1102 16-17 0.7445641675761934 1102 18-19 0.7371713754673905 1102 20-21 0.7061166246103028 1102 22-23 0.6472377528857081 1102 24-25 0.7039459985765575 1102 26-27 0.676275229813136 1102 28-29 0.6571713312081684 1102 30-31 0.6671214163192971 1102 32-33 0.6784507628560803 1102 34-35 0.6268742308571689 1102 36-37 0.6319471829645309 1102 38-39 0.6640104524018895 1102 40-41 0.6182761636031131 1102 42-43 0.5688474131373269 1102 44-45 0.536565804796318 1102 46-47 0.6013593662499446 1102 48-49 0.6031794074485646 1102 50-51 0.5727803855091267 1102 52-53 0.6443767301548391 1102 54-55 0.6954453591350074 1102 56-57 0.6409503390863733 1102 58-59 0.6587028230834377 1102 60-61 0.6424272892457168 1102 62-63 0.6148338823928583 1102 64-65 0.5541325337429797 1102 66-67 0.5288248799093083 1102 68-69 0.5288226979474828 1102 70-71 0.5159752902088641 1102 72-73 0.4997618954457508 1102 74-75 0.45316404932275134 1102 76-77 0.4424480066647831 1102 78-79 0.4122033680843842 1102 80-81 0.4289765774844625 1102 82-83 0.39878950592203566 1102 84-85 0.36688992267127674 1102 86-87 0.3328407077066764 1102 88-89 0.32917366569549955 1102 90-91 0.2567741234647727 1102 92-93 0.19560400757574925 1102 94-95 0.18373083245434074 1102 96-97 0.1775497510716626 1102 98-99 0.16158799852978945 1102 100 0.1852791876451647 1103 1 0.6042656543986631 1103 2 0.60257769006191 1103 3 0.5847045949292493 1103 4 0.5392945108749672 1103 5 0.5181239158748809 1103 6 0.5403486805458542 1103 7 0.5367807069546231 1103 8 0.5644927453646744 1103 9 0.6758102351964581 1103 10-11 0.6333773132123426 1103 12-13 0.6153037658570071 1103 14-15 0.7744748857554598 1103 16-17 0.7652154057656944 1103 18-19 0.7479125592363616 1103 20-21 0.6997104931292526 1103 22-23 0.685591887057285 1103 24-25 0.7052816595022051 1103 26-27 0.7241456411576834 1103 28-29 0.6798210425390323 1103 30-31 0.6958967990820568 1103 32-33 0.6829532689732858 1103 34-35 0.6681616065240803 1103 36-37 0.6655055318936505 1103 38-39 0.7048128982799895 1103 40-41 0.6344775044552122 1103 42-43 0.6841164690018857 1103 44-45 0.6662691383170838 1103 46-47 0.7134027681775734 1103 48-49 0.6983725739499533 1103 50-51 0.6335429799475136 1103 52-53 0.7162778706514459 1103 54-55 0.7738891033672672 1103 56-57 0.7873511384753584 1103 58-59 0.8462599139258984 1103 60-61 0.7721587284657545 1103 62-63 0.7151526309306391 1103 64-65 0.6927147770256781 1103 66-67 0.649326925740425 1103 68-69 0.6222837755216659 1103 70-71 0.6474413967738073 1103 72-73 0.6532918743979295 1103 74-75 0.6366129061570689 1103 76-77 0.6391780861501033 1103 78-79 0.613209211123646 1103 80-81 0.5517345603193746 1103 82-83 0.5129388943834847 1103 84-85 0.4828404528439165 1103 86-87 0.49545131264831 1103 88-89 0.4778639255744892 1103 90-91 0.45604523899155325 1103 92-93 0.4228678810560105 1103 94-95 0.44126002539880105 1103 96-97 0.40829215947411157 1103 98-99 0.3966006222234739 1103 100 0.40165663077485547 1104 1 0.5314930838823564 1104 2 0.5158051538337709 1104 3 0.48633752014972487 1104 4 0.44548442308352065 1104 5 0.46141817150809317 1104 6 0.4539661787934648 1104 7 0.4356213489855918 1104 8 0.47858723257702707 1104 9 0.5794770555679563 1104 10-11 0.5512595056476997 1104 12-13 0.545370458280928 1104 14-15 0.6804491652142346 1104 16-17 0.6455572438026493 1104 18-19 0.659891239707413 1104 20-21 0.5941006794069139 1104 22-23 0.5957334564054051 1104 24-25 0.638584876313125 1104 26-27 0.636414892030551 1104 28-29 0.6271052165299338 1104 30-31 0.5999511280408001 1104 32-33 0.6024545825818066 1104 34-35 0.6095341404278756 1104 36-37 0.6047469305973507 1104 38-39 0.6487106548230983 1104 40-41 0.6108088370332894 1104 42-43 0.6113168683710484 1104 44-45 0.5740678861387067 1104 46-47 0.6340973976405166 1104 48-49 0.6363361305370034 1104 50-51 0.585295810294383 1104 52-53 0.5789681294305495 1104 54-55 0.7111859452439688 1104 56-57 0.7338431023810159 1104 58-59 0.7498466205715104 1104 60-61 0.7308792600142624 1104 62-63 0.7040077626833394 1104 64-65 0.6735700957917814 1104 66-67 0.6805649441394266 1104 68-69 0.6738212101705159 1104 70-71 0.6666669730277377 1104 72-73 0.6312999885462105 1104 74-75 0.6193420213583849 1104 76-77 0.6027130100391318 1104 78-79 0.5613777745934954 1104 80-81 0.577813165099883 1104 82-83 0.5430859509025616 1104 84-85 0.5253320015272465 1104 86-87 0.5471166131895835 1104 88-89 0.5823584108051989 1104 90-91 0.4812119447480825 1104 92-93 0.4997172134397019 1104 94-95 0.5269603730027868 1104 96-97 0.5165781498585282 1104 98-99 0.42213258300112244 1104 100 0.4316896760553881 1105 1 0.4250501531105151 1105 2 0.4031231952196599 1105 3 0.39715104359691367 1105 4 0.3565992820851349 1105 5 0.375963883901008 1105 6 0.38652395996627575 1105 7 0.35507684823929253 1105 8 0.40634683783198255 1105 9 0.4640016217982108 1105 10-11 0.4581610618413592 1105 12-13 0.4602870439361837 1105 14-15 0.5576519474171917 1105 16-17 0.5370278649276301 1105 18-19 0.5403907010226803 1105 20-21 0.5243214207661211 1105 22-23 0.5236645767584562 1105 24-25 0.5156486942091618 1105 26-27 0.5176972595435032 1105 28-29 0.5005010540763237 1105 30-31 0.5179757998615386 1105 32-33 0.5024357171144089 1105 34-35 0.5045851469415439 1105 36-37 0.5119191850963389 1105 38-39 0.5002037028259352 1105 40-41 0.45308392894647653 1105 42-43 0.48998181483059255 1105 44-45 0.48965628144173223 1105 46-47 0.503841357396773 1105 48-49 0.5187358132165798 1105 50-51 0.4705261690338105 1105 52-53 0.4888352478829958 1105 54-55 0.5827141245626706 1105 56-57 0.5805291721724046 1105 58-59 0.6120701212501771 1105 60-61 0.5679240447096277 1105 62-63 0.530937107129418 1105 64-65 0.47751185339772206 1105 66-67 0.4677137814922361 1105 68-69 0.4841010101050429 1105 70-71 0.5011168423937207 1105 72-73 0.5355143020336115 1105 74-75 0.537008288803932 1105 76-77 0.48088571117119017 1105 78-79 0.45605242235751575 1105 80-81 0.4681171428238571 1105 82-83 0.47041989797949313 1105 84-85 0.4163786640287839 1105 86-87 0.36933847126725894 1105 88-89 0.39011787416997734 1105 90-91 0.35985929896586555 1105 92-93 0.3736231683851443 1105 94-95 0.39538750716783966 1105 96-97 0.35493364504071323 1105 98-99 0.3667280171185183 1105 100 0.3488970966843574 1106 1 0.31859116550804245 1106 2 0.2927735404247578 1106 3 0.27040364538965633 1106 4 0.2884274621502101 1106 5 0.29477309558019016 1106 6 0.31399307655190967 1106 7 0.2799523860265296 1106 8 0.31922563527275116 1106 9 0.3888141026076042 1106 10-11 0.37038281028309683 1106 12-13 0.3590086132534296 1106 14-15 0.4025866211500144 1106 16-17 0.41795635957585375 1106 18-19 0.41560112473637645 1106 20-21 0.4011782537929989 1106 22-23 0.3646779295461542 1106 24-25 0.4052745733893488 1106 26-27 0.38062128095562997 1106 28-29 0.38888653992500366 1106 30-31 0.36723511251339147 1106 32-33 0.34809006759559935 1106 34-35 0.3058799401839991 1106 36-37 0.3706173015415928 1106 38-39 0.3772494251560019 1106 40-41 0.3663664638221462 1106 42-43 0.3071913294133495 1106 44-45 0.2775446350501838 1106 46-47 0.32775345256622046 1106 48-49 0.36051025055157027 1106 50-51 0.3320000774765717 1106 52-53 0.38363080784260006 1106 54-55 0.38407826669768497 1106 56-57 0.39850200101159317 1106 58-59 0.4312595768593397 1106 60-61 0.4493841233990352 1106 62-63 0.41491425775595303 1106 64-65 0.3156829863282766 1106 66-67 0.3834110154067716 1106 68-69 0.35375069222535416 1106 70-71 0.4052286703614172 1106 72-73 0.34983360944620046 1106 74-75 0.3305596478333115 1106 76-77 0.36837612263501995 1106 78-79 0.334551298070771 1106 80-81 0.27228045498743114 1106 82-83 0.29570780129152396 1106 84-85 0.23544198126344895 1106 86-87 0.2585066973538339 1106 88-89 0.2163349866573192 1106 90-91 0.22995133660601397 1106 92-93 0.246916270139522 1106 94-95 0.2858483719976661 1106 96-97 0.23978947363163527 1106 98-99 0.212463206358791 1106 100 0.2815335308507443 1107 1 0.46418462329507904 1107 2 0.45600585111067815 1107 3 0.4074717233112821 1107 4 0.4366185141400649 1107 5 0.43901495160976367 1107 6 0.4471791027398808 1107 7 0.4236629496733926 1107 8 0.42072718652011076 1107 9 0.5331338187042434 1107 10-11 0.5208997725704805 1107 12-13 0.5011992672565739 1107 14-15 0.5997498230782128 1107 16-17 0.6127371919707016 1107 18-19 0.604905055384215 1107 20-21 0.582223136069338 1107 22-23 0.5548672193256294 1107 24-25 0.5853284178102598 1107 26-27 0.5745235973285432 1107 28-29 0.5652182791461442 1107 30-31 0.5835769970330489 1107 32-33 0.5721755670190305 1107 34-35 0.5534188335725716 1107 36-37 0.5443799417050244 1107 38-39 0.5927717814576923 1107 40-41 0.5744004539832446 1107 42-43 0.5961865735633864 1107 44-45 0.5477571538542421 1107 46-47 0.5883113263590829 1107 48-49 0.5947533035028414 1107 50-51 0.5165898525401786 1107 52-53 0.5591619153478646 1107 54-55 0.612717188505826 1107 56-57 0.6187042852294979 1107 58-59 0.6332569041881513 1107 60-61 0.6319634994831915 1107 62-63 0.6154141064296894 1107 64-65 0.5550059007385997 1107 66-67 0.5258253909497697 1107 68-69 0.5862365258314028 1107 70-71 0.5705443680553586 1107 72-73 0.5340268080681128 1107 74-75 0.5474763150060653 1107 76-77 0.5599044815084895 1107 78-79 0.5471286404148827 1107 80-81 0.5561053149386908 1107 82-83 0.4895435595808344 1107 84-85 0.510906087111465 1107 86-87 0.46326559790710675 1107 88-89 0.5230363326012544 1107 90-91 0.5186449242285818 1107 92-93 0.5115923396921183 1107 94-95 0.5007999219436172 1107 96-97 0.5185070005254886 1107 98-99 0.5295662321992296 1107 100 0.47894640877627026 1108 1 0.22984874299857694 1108 2 0.18997946511013097 1108 3 0.23899378035959984 1108 4 0.2623135684395237 1108 5 0.2536338117961279 1108 6 0.2758137284774449 1108 7 0.2657087892500982 1108 8 0.2706369095899319 1108 9 0.28234031654533 1108 10-11 0.2808350173978269 1108 12-13 0.2754476163880142 1108 14-15 0.3548799773798308 1108 16-17 0.35091059224374277 1108 18-19 0.33581453264803685 1108 20-21 0.29482948858675684 1108 22-23 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80-81 -1.5645518103081457 2316 82-83 -1.82134515752767 2316 84-85 -1.5531374046237971 2316 86-87 -1.454299523413841 2316 88-89 -1.616536071950673 2316 90-91 -1.512741959103117 2316 92-93 -1.4540862283110876 2316 94-95 -1.4357631215847633 2316 96-97 -1.31468397207054 2316 98-99 -1.3792913869881787 2316 100 -1.551748884943141 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 44.0 4 105.0 5 181.0 6 1994.0 7 23976.0 8 39030.0 9 49727.0 10 52955.0 11 51894.0 12 52297.0 13 57023.0 14 66414.0 15 80530.0 16 95574.0 17 111757.0 18 123184.0 19 137187.0 20 154700.0 21 186248.0 22 234469.0 23 317940.0 24 436745.0 25 523407.0 26 521980.0 27 467269.0 28 439929.0 29 468573.0 30 537377.0 31 643505.0 32 794938.0 33 1018491.0 34 1375634.0 35 2022211.0 36 3304535.0 37 5991178.0 38 7835424.0 39 2636798.0 40 6549.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.215560243347948 34.747403833834994 27.56392642609543 20.473109496721626 2 20.981173001984253 32.081860030040545 28.111054606668368 18.825912361306834 3 24.197129857699625 27.048250773015152 27.257748484340716 21.496870884944503 4 19.673998227060085 27.591025831536232 27.15563227911951 25.579343662284177 5 20.676394744872866 32.10224332547437 26.90304655034993 20.31831537930283 6 25.617621697159837 26.944713349576947 27.078603911661325 20.35906104160189 7 20.53676632696269 27.337934451722347 31.86804698058167 20.257252240733294 8 25.042278842575854 27.0386580524281 27.31462730007856 20.604435804917486 9 25.113755619735638 26.802106502504135 27.512279593018835 20.571858284741396 10-11 20.187025235849234 29.813243705294813 29.687291763003493 20.312439295852464 12-13 20.214591807413683 27.275037428200783 29.572503910665354 22.937866853720184 14-15 20.242071647732995 32.420612465155926 26.937111712185548 20.400204174925534 16-17 22.901915677427727 27.20249504791883 26.90792673862019 22.987662536033252 18-19 20.34811708154671 29.76151045377433 26.85599517746421 23.034377287214745 20-21 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27.437054798276282 31.377123391983364 20.488323936208833 46-47 20.692439275006112 27.524532744516346 31.325782243007865 20.45724573746968 48-49 20.781721421493128 27.514199209599493 31.21680245029116 20.48727691861622 50-51 20.809725943466532 27.556672361454098 31.158743578454768 20.47485811662461 52-53 20.892936386789724 27.71248881854018 30.959432253046753 20.435142541623343 54-55 21.227530771163025 27.880558459132494 30.321703899976498 20.570206869727983 56-57 21.589429533989097 28.29859396815113 29.438588956268774 20.673387541590998 58-59 21.757644599317818 28.710345290891485 28.889868710320982 20.64214139946971 60-61 21.860844765455674 28.94546962060095 28.546361397388438 20.64732421655494 62-63 21.918352797098226 29.05579425237586 28.424670977667173 20.601181972858743 64-65 22.047359567370144 29.097449816176717 28.384511086381735 20.4706795300714 66-67 22.169668209033976 28.928808375556198 28.49433630569274 20.40718710971709 68-69 22.270332291727165 28.76137101681198 28.65333246729589 20.314964224164964 70-71 22.373984103546977 28.664206333441367 28.757875347709078 20.20393421530258 72-73 22.526094566772226 28.62634144266658 28.719616907951394 20.127947082609797 74-75 22.686251446270386 28.660910485813453 28.60521628097214 20.047621786944024 76-77 22.950324644168724 28.749952992406165 28.331760470050003 19.967961893375104 78-79 23.166212522135545 28.824020000458695 28.03656150498443 19.973205972421326 80-81 23.392264910399994 28.896954400450976 27.79727036118458 19.913510327964453 82-83 23.574437771323897 28.989059569543475 27.58519093458196 19.851311724550666 84-85 23.7107564251196 29.0132340320186 27.43446333510678 19.841546207755012 86-87 23.90167877862812 28.978339688829948 27.293484020797187 19.82649751174474 88-89 24.057640625699847 28.985516408963868 27.161682734175802 19.795160231160484 90-91 24.22902286005838 28.945566484396245 27.02522594008135 19.800184715464024 92-93 24.380574720532316 28.917177831471662 26.973488309833353 19.72875913816267 94-95 24.540018753807587 28.924605929567708 26.90107699608852 19.634298320536182 96-97 24.64252004743149 28.895446822727955 26.812626776380927 19.649406353459632 98-99 24.734655374956766 28.885097256963597 26.746850696558518 19.633396671521123 100 24.79381944290904 28.941890789029458 26.6434161267213 19.620873641340204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 71188.0 1 44006.5 2 14045.0 3 10692.5 4 10205.0 5 10412.5 6 10821.5 7 11093.0 8 11351.0 9 11822.5 10 12858.5 11 15174.5 12 94052.5 13 116606.0 14 61833.0 15 66286.0 16 74984.5 17 83531.5 18 92698.0 19 102586.5 20 115000.0 21 136488.5 22 167772.5 23 201178.0 24 231979.5 25 268734.0 26 310751.0 27 357848.0 28 422120.0 29 484107.5 30 529924.5 31 585056.0 32 647392.0 33 693576.0 34 734806.0 35 791920.5 36 853979.0 37 892762.0 38 925991.0 39 954130.5 40 967464.5 41 993056.0 42 1034004.5 43 1043800.5 44 1027465.5 45 1030629.5 46 1035486.5 47 1032190.0 48 1020600.5 49 1003532.0 50 985240.0 51 963019.0 52 933021.5 53 888641.0 54 834849.5 55 772366.5 56 701392.5 57 629641.0 58 556581.5 59 474572.5 60 390802.0 61 318540.5 62 252170.0 63 189895.0 64 140619.0 65 103184.0 66 75432.5 67 56745.5 68 42742.5 69 32368.5 70 25460.0 71 20990.0 72 17832.5 73 14388.5 74 11837.0 75 10562.0 76 8291.5 77 5270.5 78 3469.5 79 2758.0 80 2285.0 81 1913.5 82 1616.0 83 1349.5 84 1128.0 85 976.0 86 823.5 87 701.0 88 624.0 89 559.5 90 490.5 91 425.0 92 366.5 93 315.0 94 267.5 95 232.5 96 193.0 97 153.0 98 122.5 99 81.0 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04778727546817468 2 0.015579144321330611 3 0.005732010462652631 4 1.2312967641650647E-4 5 5.767653263720567E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.240254642539644E-6 12-13 5.1844074280634304E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.03844886158837542 22-23 0.07527597572815975 24-25 0.031469353088345024 26-27 0.03579023265417164 28-29 0.040339550172297296 30-31 0.03397569005434944 32-33 0.03473390964070371 34-35 0.035581236229727836 36-37 0.0333551812903031 38-39 0.04354254188644774 40-41 0.06388000015034781 42-43 0.07200655879383724 44-45 0.0747947979137426 46-47 0.048072417876718156 48-49 0.030583143443610433 50-51 0.028201556281343795 52-53 0.028519101236312676 54-55 0.02923357738499267 56-57 0.029462015337291717 58-59 0.03719650316903384 60-61 0.056725517899620284 62-63 0.08025948736838572 64-65 0.10818076162314982 66-67 0.08933058024017544 68-69 0.05281777080071747 70-71 0.04641502762705913 72-73 0.05023690797793465 74-75 0.04781481763263626 76-77 0.05084607585073209 78-79 0.04367701245411313 80-81 0.042129790862300454 82-83 0.041484980188435064 84-85 0.04876745249754291 86-87 0.052370615660047 88-89 0.05834240496624757 90-91 0.05519611770834156 92-93 0.05093356272608066 94-95 0.052824251310002554 96-97 0.054632313400539666 98-99 0.04921622776553465 100 0.0510340106199994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0861772E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.68672201329212 #Duplication Level Percentage of deduplicated Percentage of total 1 74.21260368423907 31.678927833517324 2 13.147858651476643 11.224779746512825 3 4.503521476953354 5.767217081027956 4 2.187274105322897 3.7347024680316294 5 1.3039573635131274 2.783083274673509 6 0.8725584105365685 2.2347994986560713 7 0.6507025571146949 1.9443451418225326 8 0.4500447156962294 1.5368746937980826 9 0.35003199353456077 1.3447546563391435 >10 1.9860096270506087 15.754003904304223 >50 0.19347985520262956 5.719921677723937 >100 0.14112864125068245 10.966994032619066 >500 3.599617243440681E-4 0.09406857564815262 >1k 4.237765735137851E-4 0.36898509152482933 >5k 1.5059937050055216E-5 0.03975763627609359 >10k+ 3.0119874081116244E-5 4.806784687524638 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1345665 4.36029726355311 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104660 0.33912505088819916 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025461920981076525 6.480509285079289E-6 0.0 0.0 0.0 2 0.025889634593891757 6.480509285079289E-6 0.0 0.0 3.2402546425396444E-6 3 0.02641131559134064 6.480509285079289E-6 0.0 0.0 1.2961018570158578E-5 4 0.026421036355268258 1.620127321269822E-5 0.0 0.0 1.9441527855237865E-5 5 0.026421036355268258 3.564280106793609E-5 0.0 0.0 1.9441527855237865E-5 6 0.026427516864553337 4.536356499555502E-5 0.0 0.0 2.5922037140317155E-5 7 0.026446958392408577 5.508432892317395E-5 0.0 0.0 2.5922037140317155E-5 8 0.026563607559540004 6.156483820825324E-5 0.0 0.0 3.564280106793609E-5 9 0.0265960101059654 7.128560213587218E-5 0.0 3.2402546425396444E-6 3.888305571047573E-5 10-11 0.026628412652390795 7.452585677841182E-5 0.0 6.480509285079289E-6 4.536356499555502E-5 12-13 0.026646234052924767 8.910700266984022E-5 0.0 6.480509285079289E-6 4.860381963809467E-5 14-15 0.026662435326137462 9.072712999111004E-5 0.0 6.480509285079289E-6 5.994471088698342E-5 16-17 0.02668835736327778 9.234725731237986E-5 0.0 6.480509285079289E-6 8.262649338476092E-5 18-19 0.0267353410555946 9.882776659745916E-5 0.0 6.480509285079289E-6 1.0368814856126862E-4 20-21 0.026754782583449842 1.1826929445269701E-4 0.0 1.1340891248888755E-5 1.247498037377763E-4 22-23 0.026840649331477145 1.2961018570158578E-4 0.0 1.2961018570158578E-5 1.4095107695047453E-4 24-25 0.02714847352251841 1.393309496292047E-4 0.0 1.2961018570158578E-5 1.6039260480571238E-4 26-27 0.02832630608508157 1.5553222284190292E-4 0.0 1.620127321269822E-5 1.701133687333313E-4 28-29 0.032305338786120255 1.620127321269822E-4 0.0 1.9441527855237865E-5 1.8469451462475972E-4 30-31 0.04264661147778553 1.7983413266095026E-4 0.0 2.9162291782856797E-5 1.9279515123110883E-4 32-33 0.06458799578974272 1.9279515123110883E-4 0.0 3.888305571047573E-5 2.1061655176507688E-4 34-35 0.10263668592976449 2.122366790863467E-4 0.0 4.698369231682484E-5 2.1709706105015617E-4 36-37 0.16205485543733522 2.3653858890539402E-4 0.0 4.860381963809466E-5 2.18717188371426E-4 38-39 0.24849836879100784 2.511197347968224E-4 0.0 5.022394695936449E-5 2.3653858890539402E-4 40-41 0.3626833222667837 2.673210080095206E-4 0.0 5.184407428063431E-5 2.511197347968224E-4 42-43 0.5083424892128683 2.7218138997333013E-4 0.0 5.9944710886983415E-5 2.6084049872444133E-4 44-45 0.6865467737886211 2.867625358647585E-4 0.0 6.480509285079288E-5 2.754216446158698E-4 46-47 0.8988855208962078 3.078241910412662E-4 0.0 6.480509285079288E-5 2.867625358647585E-4 48-49 1.1472996430665097 3.1106444568380584E-4 0.0 6.480509285079288E-5 2.9810342711364727E-4 50-51 1.4319932763420065 3.175449549688851E-4 0.0 6.804534749333253E-5 3.0620406371999637E-4 52-53 1.7521207142609958 3.240254642539644E-4 0.0 8.10063660634911E-5 3.1106444568380584E-4 54-55 2.104593994149137 3.305059735390437E-4 0.0 8.10063660634911E-5 3.1916508229015494E-4 56-57 2.4905504453859617 3.418468647879325E-4 0.0 8.10063660634911E-5 3.2726571889650404E-4 58-59 2.9075533964802798 3.564280106793609E-4 0.0 8.262649338476092E-5 3.3374622818158333E-4 60-61 3.3558345256390334 3.7910979317713837E-4 0.0 8.424662070603074E-5 3.4670724675174195E-4 62-63 3.833177498686725 3.9369093906856676E-4 0.0 8.74868753485704E-5 3.596682653219005E-4 64-65 4.336753573320417 4.1151233960253484E-4 0.0 9.072712999111004E-5 3.742494112133289E-4 66-67 4.869248920638777 4.2933374013650287E-4 0.0 9.55875119549195E-5 3.807299204984082E-4 68-69 5.425449970921955 4.406746313853916E-4 0.0 1.0692840320380826E-4 3.888305571047573E-4 70-71 6.005204432201754 4.536356499555502E-4 0.0 1.1178878516761773E-4 3.969311937111064E-4 72-73 6.606180941262867 4.6011615924062947E-4 0.0 1.2961018570158576E-4 4.147525942450745E-4 74-75 7.231227681936086 4.7469730513205786E-4 0.0 1.3447056766539525E-4 4.2609348549396323E-4 76-77 7.876974789393168 4.844180690596768E-4 0.0 1.393309496292047E-4 4.37434376742852E-4 78-79 8.539754295378762 5.038595969149146E-4 0.0 1.45811458914284E-4 4.4229475870666144E-4 80-81 9.22114258377646 5.233011247701524E-4 0.0 1.4905171355682363E-4 4.471551406704709E-4 82-83 9.918246107190473 5.330218886977715E-4 0.0 1.5553222284190292E-4 4.5849603191935965E-4 84-85 10.635863682746407 5.638043078018981E-4 0.0 1.5553222284190292E-4 4.714570504895182E-4 86-87 11.367761060512015 5.783854536933265E-4 0.0 1.5877247748444256E-4 4.8117781441713714E-4 88 11.923605682784514 5.962068542272945E-4 0.0 1.5877247748444256E-4 4.8279794173840696E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 166785 0.0 87.10666 8 GTGGTAT 169965 0.0 85.42717 6 GTATCAA 170620 0.0 85.09096 9 TGGTATC 170285 0.0 85.087326 7 AGTGGTA 171460 0.0 84.71587 5 GCAGTGG 180675 0.0 81.07538 3 AAGCAGT 181680 0.0 80.24623 1 CAGTGGT 182830 0.0 79.4629 4 AGCAGTG 190765 0.0 76.79643 2 CAACGCA 163755 0.0 45.345955 12-13 ATCAACG 165825 0.0 44.82947 10-11 TCAACGC 163365 0.0 44.723885 12-13 ACGCAGA 167095 0.0 44.434017 14-15 AACGCAG 165435 0.0 44.236523 14-15 AGAGTAC 167475 0.0 43.692356 18-19 CGCAGAG 168400 0.0 43.34667 16-17 GAGTACT 165005 0.0 43.197903 20-21 TATCAAC 170525 0.0 42.5802 10-11 AGTACTT 175095 0.0 41.435097 20-21 GTACTTT 175245 0.0 40.680443 22-23 >>END_MODULE