FastQCFastQC Report
Thu 26 May 2016
SRR1660758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30861772
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14721054.769995060555822No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA802800.26012764270308264No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350810.11367137311493326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1820200.087.36068
GTGGTAT1856100.085.820286
TGGTATC1861200.085.400817
GTATCAA1858350.085.390179
AGTGGTA1868800.085.23435
GCAGTGG1946750.081.9203953
AAGCAGT1919850.081.207071
CAGTGGT1973800.080.5358054
AGCAGTG2045600.077.830782
CAACGCA1776400.045.41869412-13
ATCAACG1801250.044.87554610-11
TCAACGC1771550.044.76979412-13
ACGCAGA1811800.044.6276814-15
AACGCAG1790150.044.36393714-15
AGAGTAC1837050.043.8296618-19
CGCAGAG1832400.043.4487716-17
GAGTACT1810350.043.3808320-21
TATCAAC1850300.042.8744910-11
AGTACTT1917700.041.6828320-21
GTACTTT1918300.040.90048622-23
Produced by FastQC (version 0.11.5)