##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660758_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30861772 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.993723756367586 33.0 31.0 34.0 27.0 34.0 2 31.78155988580306 34.0 31.0 34.0 28.0 34.0 3 32.28991630811089 34.0 31.0 34.0 30.0 34.0 4 35.822796079240035 37.0 35.0 37.0 35.0 37.0 5 35.88108294624171 37.0 35.0 37.0 35.0 37.0 6 35.9187720329215 37.0 35.0 37.0 35.0 37.0 7 35.9205559875175 37.0 35.0 37.0 35.0 37.0 8 35.92289078540273 37.0 35.0 37.0 35.0 37.0 9 37.63587998122726 39.0 37.0 39.0 35.0 39.0 10-11 37.581801151923486 39.0 37.0 39.0 35.0 39.0 12-13 37.63386647079111 39.0 37.0 39.0 35.0 39.0 14-15 38.96016034011268 40.0 38.0 41.0 35.0 41.0 16-17 38.975674889957716 40.0 38.0 41.0 35.0 41.0 18-19 38.96216584711986 40.0 38.0 41.0 35.0 41.0 20-21 38.90823065830439 40.0 38.0 41.0 35.0 41.0 22-23 38.81478733625535 40.0 38.0 41.0 34.5 41.0 24-25 38.756006897465255 40.0 38.0 41.0 34.0 41.0 26-27 38.708077455824636 40.0 38.0 41.0 34.0 41.0 28-29 38.60608281015102 40.0 38.0 41.0 34.0 41.0 30-31 38.512911410271585 40.0 38.0 41.0 34.0 41.0 32-33 38.41605676433615 40.0 38.0 41.0 34.0 41.0 34-35 38.312367028050105 40.0 38.0 41.0 34.0 41.0 36-37 38.139973945760474 40.0 38.0 41.0 33.0 41.0 38-39 37.76245199076709 40.0 36.5 41.0 33.0 41.0 40-41 37.62262717772654 40.0 36.0 41.0 33.0 41.0 42-43 37.47465898912091 40.0 36.0 41.0 33.0 41.0 44-45 37.36412816801317 40.0 36.0 41.0 32.0 41.0 46-47 37.240806344496356 39.0 35.0 41.0 32.0 41.0 48-49 37.37493953684837 40.0 36.0 41.0 32.0 41.0 50-51 37.29813704475556 40.0 35.5 41.0 32.0 41.0 52-53 37.069388838074495 39.0 35.0 41.0 31.0 41.0 54-55 36.62962157519665 39.0 35.0 41.0 30.0 41.0 56-57 36.37506370340627 39.0 35.0 41.0 29.0 41.0 58-59 36.20109038133002 39.0 35.0 41.0 28.0 41.0 60-61 36.016820421069795 39.0 35.0 41.0 28.0 41.0 62-63 35.74005219142958 38.5 35.0 41.0 28.0 41.0 64-65 35.50493656035046 38.0 35.0 40.0 28.0 41.0 66-67 35.24980344615338 37.0 34.5 40.0 28.0 41.0 68-69 34.971082477052846 37.0 34.0 40.0 27.0 41.0 70-71 34.65257871453396 36.5 34.0 39.0 26.5 41.0 72-73 34.34427509541578 36.0 34.0 39.0 26.0 41.0 74-75 33.99347838808478 35.5 34.0 38.5 26.0 40.0 76-77 31.894844923356963 34.0 31.0 36.0 23.5 39.0 78-79 33.1700094213644 35.0 33.0 37.0 26.0 39.0 80-81 33.15478430726531 35.0 33.5 36.5 26.0 39.0 82-83 32.93182836358197 35.0 33.0 36.0 26.0 38.0 84-85 32.69303721445418 35.0 33.0 36.0 25.5 37.0 86-87 32.484010623239655 35.0 33.0 35.5 25.0 37.0 88-89 32.26198879636594 35.0 33.0 35.0 25.0 36.5 90-91 32.100392193941424 35.0 33.0 35.0 25.0 36.0 92-93 31.97448335111801 35.0 33.0 35.0 24.0 36.0 94-95 31.876918830195493 35.0 33.0 35.0 24.0 36.0 96-97 31.775573904181524 35.0 33.0 35.0 24.0 35.5 98-99 31.66294373829215 35.0 33.0 35.0 23.5 35.0 100 31.47916091791489 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.754446617581511 1101 2 1.2149949862750944 1101 3 0.7703842765916562 1101 4 0.6229318372148214 1101 5 0.478258018020135 1101 6 0.44031676113979046 1101 7 0.3950682657196438 1101 8 0.40576020921814404 1101 9 0.48597270557126393 1101 10-11 0.49078936106005244 1101 12-13 0.4583490849993339 1101 14-15 0.6232051688194815 1101 16-17 0.5842489685679908 1101 18-19 0.5344068959969022 1101 20-21 0.5735898258922489 1101 22-23 0.5451095520943738 1101 24-25 0.6029352215851915 1101 26-27 0.6085247817852846 1101 28-29 0.6073904469360372 1101 30-31 0.5986495997234229 1101 32-33 0.5993143186765906 1101 34-35 0.5825537225002577 1101 36-37 0.5715652840235705 1101 38-39 0.6448832128341806 1101 40-41 0.6429773473385794 1101 42-43 0.5946052406842668 1101 44-45 0.6168788873114934 1101 46-47 0.5863936890976049 1101 48-49 0.553413818866467 1101 50-51 0.5395872192592179 1101 52-53 0.5636276862688732 1101 54-55 0.6037450199351966 1101 56-57 0.646439437502984 1101 58-59 0.6070755392791298 1101 60-61 0.5736898010711897 1101 62-63 0.6391887315385318 1101 64-65 0.6210802319608035 1101 66-67 0.5712527255437507 1101 68-69 0.5561915789877503 1101 70-71 0.5634822442699559 1101 72-73 0.5133985101931202 1101 74-75 0.5251347627309428 1101 76-77 0.41460981417601417 1101 78-79 0.5429504282250832 1101 80-81 0.5214733940424807 1101 82-83 0.4748528908320395 1101 84-85 0.49075653896412774 1101 86-87 0.44727774004905285 1101 88-89 0.4955093216058728 1101 90-91 0.5154528058533288 1101 92-93 0.40588687472970264 1101 94-95 0.3549957248720723 1101 96-97 0.3866506572616224 1101 98-99 0.39826168127364525 1101 100 0.33673222182035545 1102 1 1.6693534138959798 1102 2 1.145514726119366 1102 3 0.7066323070868137 1102 4 0.55367162222079 1102 5 0.42520170040143057 1102 6 0.3911130372653844 1102 7 0.34140037114190136 1102 8 0.363943687180722 1102 9 0.44958011814384946 1102 10-11 0.4547717687693904 1102 12-13 0.37260565424404746 1102 14-15 0.5385774354965776 1102 16-17 0.5194944978780498 1102 18-19 0.5204175980718801 1102 20-21 0.5210344593691971 1102 22-23 0.5333822084398037 1102 24-25 0.5468351234023814 1102 26-27 0.5304109866728552 1102 28-29 0.5211355498132519 1102 30-31 0.532010921125476 1102 32-33 0.5378319259599706 1102 34-35 0.5212407142226567 1102 36-37 0.5718480455516755 1102 38-39 0.6209149047813085 1102 40-41 0.5668706110464399 1102 42-43 0.6013785832385778 1102 44-45 0.5605755393814675 1102 46-47 0.5808652593757984 1102 48-49 0.5300479155907212 1102 50-51 0.5107508471319235 1102 52-53 0.44906843001732 1102 54-55 0.5009077935912885 1102 56-57 0.5626869484889667 1102 58-59 0.5555632318257082 1102 60-61 0.5398107302998483 1102 62-63 0.5696750242265836 1102 64-65 0.5536755160597409 1102 66-67 0.5092157496278489 1102 68-69 0.4891706101089852 1102 70-71 0.5253663216573514 1102 72-73 0.4389095467932762 1102 74-75 0.42545841023207487 1102 76-77 0.35540308182308067 1102 78-79 0.4489414362702462 1102 80-81 0.46754492000656 1102 82-83 0.4788748439977084 1102 84-85 0.443578293326766 1102 86-87 0.39633696345959635 1102 88-89 0.4212913914641163 1102 90-91 0.41774361261155946 1102 92-93 0.37472924723093115 1102 94-95 0.3207590712419979 1102 96-97 0.3146865713859519 1102 98-99 0.36679779353411845 1102 100 0.2702296198892107 1103 1 1.6593830458643914 1103 2 1.0961834396936325 1103 3 0.6710371792210736 1103 4 0.539175584903326 1103 5 0.40812805664268126 1103 6 0.3602333891673979 1103 7 0.3166288187801882 1103 8 0.35572128082645094 1103 9 0.4460569395486189 1103 10-11 0.39855890186289855 1103 12-13 0.3825129139139918 1103 14-15 0.5284084664311379 1103 16-17 0.5041558635405821 1103 18-19 0.43029636507709057 1103 20-21 0.44326871797810696 1103 22-23 0.461522058970516 1103 24-25 0.514086363149751 1103 26-27 0.4963094893927362 1103 28-29 0.49554961091707384 1103 30-31 0.49843229707183667 1103 32-33 0.4919852175806412 1103 34-35 0.49503902903619235 1103 36-37 0.48918226742009807 1103 38-39 0.5383826047218392 1103 40-41 0.5198148857222193 1103 42-43 0.4756027423246607 1103 44-45 0.5055691177625761 1103 46-47 0.5034053917406709 1103 48-49 0.49283179790796083 1103 50-51 0.42726262541761884 1103 52-53 0.4634156221906949 1103 54-55 0.5162143998436761 1103 56-57 0.5415728870283303 1103 58-59 0.509660465508972 1103 60-61 0.500181863334312 1103 62-63 0.5232294712404055 1103 64-65 0.49160559286847416 1103 66-67 0.45619631590172816 1103 68-69 0.4824288367896017 1103 70-71 0.5202810999026894 1103 72-73 0.45260900177350294 1103 74-75 0.40951239087070945 1103 76-77 0.2892385650887199 1103 78-79 0.41275799042111316 1103 80-81 0.4592597196493813 1103 82-83 0.45818935355056567 1103 84-85 0.4329285980910669 1103 86-87 0.4085138573857776 1103 88-89 0.4253349520773355 1103 90-91 0.41078767107843106 1103 92-93 0.38644620931032136 1103 94-95 0.34190474569896523 1103 96-97 0.3191317168361145 1103 98-99 0.3543947898184001 1103 100 0.2895714572623298 1104 1 1.6022915108696871 1104 2 1.0777163358224442 1104 3 0.6245856011209483 1104 4 0.5212100985196955 1104 5 0.3836930728221688 1104 6 0.35084067962033316 1104 7 0.2971016230268546 1104 8 0.3099790379210887 1104 9 0.3922511993040487 1104 10-11 0.3812682760690933 1104 12-13 0.34643779237368477 1104 14-15 0.5096065913287049 1104 16-17 0.47966499055240774 1104 18-19 0.4023872280256384 1104 20-21 0.3960371907840923 1104 22-23 0.41584016660005574 1104 24-25 0.4947966541868922 1104 26-27 0.45993686346491813 1104 28-29 0.4559828369322574 1104 30-31 0.4293399328044032 1104 32-33 0.42805803785449115 1104 34-35 0.41000823721222446 1104 36-37 0.442063619475924 1104 38-39 0.4997376601873782 1104 40-41 0.5150166830397183 1104 42-43 0.46981574085237554 1104 44-45 0.44985714405752475 1104 46-47 0.5043949496287681 1104 48-49 0.5019134857906522 1104 50-51 0.4575514069221711 1104 52-53 0.41075486854172993 1104 54-55 0.48945357876637274 1104 56-57 0.5508189085963338 1104 58-59 0.5191639368211369 1104 60-61 0.47142040981713507 1104 62-63 0.5513762193791294 1104 64-65 0.5057961055726139 1104 66-67 0.49376194694197295 1104 68-69 0.4918794775231703 1104 70-71 0.4963562390220986 1104 72-73 0.434679512630602 1104 74-75 0.4247691259526718 1104 76-77 0.4315282619859211 1104 78-79 0.48175837505755936 1104 80-81 0.41862945781631566 1104 82-83 0.3867152095886084 1104 84-85 0.4475343420077209 1104 86-87 0.42981039587176184 1104 88-89 0.46601413357900867 1104 90-91 0.47985285429950153 1104 92-93 0.45392635531609216 1104 94-95 0.42525350441298215 1104 96-97 0.43249225719975115 1104 98-99 0.43038116504257573 1104 100 0.3629266512655285 1105 1 1.5948393714172902 1105 2 1.0525401136165726 1105 3 0.5968067888416257 1105 4 0.5079732513829143 1105 5 0.331944590407133 1105 6 0.26630670287815406 1105 7 0.23067176261164946 1105 8 0.2558124720411641 1105 9 0.32516223269142586 1105 10-11 0.2832916702856849 1105 12-13 0.2731398713931341 1105 14-15 0.3959183317949808 1105 16-17 0.31814233927161695 1105 18-19 0.3005924226660781 1105 20-21 0.32372069056088293 1105 22-23 0.30358578793634905 1105 24-25 0.3382868905914762 1105 26-27 0.3138947470867137 1105 28-29 0.3485552262088021 1105 30-31 0.35218998164663873 1105 32-33 0.39923252216909333 1105 34-35 0.38715105517891146 1105 36-37 0.40103031843631953 1105 38-39 0.465587489371444 1105 40-41 0.4355796828409666 1105 42-43 0.3966128220924787 1105 44-45 0.3784120667852875 1105 46-47 0.4199181528162512 1105 48-49 0.4094650701734608 1105 50-51 0.3781096866529339 1105 52-53 0.3639611803203877 1105 54-55 0.4134402057655677 1105 56-57 0.4245403651635016 1105 58-59 0.41668038799512175 1105 60-61 0.35531337547428876 1105 62-63 0.41691635350468204 1105 64-65 0.386205181859097 1105 66-67 0.38351906314368023 1105 68-69 0.423001060999745 1105 70-71 0.406797169076647 1105 72-73 0.3502885124609989 1105 74-75 0.29352884607775565 1105 76-77 0.2870067243372425 1105 78-79 0.41694131787014754 1105 80-81 0.35913385829234556 1105 82-83 0.3564841334560782 1105 84-85 0.3687309629179083 1105 86-87 0.36931633477801284 1105 88-89 0.39871787068884146 1105 90-91 0.38651274453066264 1105 92-93 0.3466471901919519 1105 94-95 0.32158735365646507 1105 96-97 0.31650483775544913 1105 98-99 0.34552291493179155 1105 100 0.3349843113831348 1106 1 1.4064152064909017 1106 2 0.8796588078316887 1106 3 0.4940832515892737 1106 4 0.42691229735994796 1106 5 0.2635941636607697 1106 6 0.24626618340317918 1106 7 0.17315927102563933 1106 8 0.19559189152205647 1106 9 0.2293955540323367 1106 10-11 0.20972195620810652 1106 12-13 0.19457066156952152 1106 14-15 0.3180358360324007 1106 16-17 0.23483691736759482 1106 18-19 0.19951754017261436 1106 20-21 0.2367812833677121 1106 22-23 0.2007892736981276 1106 24-25 0.24306350343886862 1106 26-27 0.22116552593728045 1106 28-29 0.2725600568755766 1106 30-31 0.24512615952638583 1106 32-33 0.224141706479017 1106 34-35 0.23371106858585478 1106 36-37 0.22597257504693147 1106 38-39 0.33267350574199384 1106 40-41 0.32876305184446863 1106 42-43 0.27508636844002865 1106 44-45 0.24907669851016578 1106 46-47 0.29523862670370704 1106 48-49 0.30119047745775873 1106 50-51 0.2591965807290393 1106 52-53 0.20582619301004001 1106 54-55 0.2821723384235142 1106 56-57 0.28341183728962704 1106 58-59 0.2746625035376482 1106 60-61 0.2626748154167089 1106 62-63 0.26995179662487345 1106 64-65 0.30134784277453264 1106 66-67 0.24537222400400793 1106 68-69 0.2599113468565264 1106 70-71 0.26369005786248323 1106 72-73 0.2722838471953466 1106 74-75 0.2263679135418286 1106 76-77 0.1649941951755558 1106 78-79 0.3109388959100414 1106 80-81 0.27853599624709346 1106 82-83 0.2701863452839248 1106 84-85 0.2734244367538139 1106 86-87 0.27125592789061415 1106 88-89 0.29525450845236634 1106 90-91 0.3143083314508104 1106 92-93 0.3041286846424569 1106 94-95 0.30073854860703264 1106 96-97 0.3258545199276419 1106 98-99 0.33690120756402564 1106 100 0.30300248661334805 1107 1 1.5766913037447061 1107 2 1.0347835440486008 1107 3 0.598975732817074 1107 4 0.4989112382127132 1107 5 0.33417399494447864 1107 6 0.33109151590911523 1107 7 0.27478726830879197 1107 8 0.2757026552790265 1107 9 0.33548814107966507 1107 10-11 0.3030475724028676 1107 12-13 0.3142023725667755 1107 14-15 0.4397489706788775 1107 16-17 0.3780627120821336 1107 18-19 0.3273271484794975 1107 20-21 0.34783831348040195 1107 22-23 0.37670854561118006 1107 24-25 0.4237279921096757 1107 26-27 0.3640714011415085 1107 28-29 0.37194307236499213 1107 30-31 0.3946353447270141 1107 32-33 0.40518493719821436 1107 34-35 0.4172616561272591 1107 36-37 0.47695008754175916 1107 38-39 0.50237526766238 1107 40-41 0.4469904985568647 1107 42-43 0.44681352666868435 1107 44-45 0.4164447088173304 1107 46-47 0.47095382388614127 1107 48-49 0.46038676258547895 1107 50-51 0.4481875826138051 1107 52-53 0.3946697821261793 1107 54-55 0.4112148203692314 1107 56-57 0.3870539564418962 1107 58-59 0.39869750828147943 1107 60-61 0.3782863432441701 1107 62-63 0.40714037651904533 1107 64-65 0.36258695932141904 1107 66-67 0.3788941035378244 1107 68-69 0.33809255309780895 1107 70-71 0.35213534031321814 1107 72-73 0.32648154708723354 1107 74-75 0.31336905194617515 1107 76-77 0.2396530200476299 1107 78-79 0.39597343740696545 1107 80-81 0.33952588962520025 1107 82-83 0.30151192110091785 1107 84-85 0.30006638600393387 1107 86-87 0.29902640299709304 1107 88-89 0.3264515691046057 1107 90-91 0.3483422450431206 1107 92-93 0.3627458181479568 1107 94-95 0.32462462850950047 1107 96-97 0.2689178728136774 1107 98-99 0.34104539083439533 1107 100 0.2578384561609539 1108 1 1.4038578443151444 1108 2 0.8950466967921713 1108 3 0.4576951198062176 1108 4 0.3884122144626261 1108 5 0.22911266231992045 1108 6 0.19300528252143323 1108 7 0.14348250375030602 1108 8 0.15219485474410988 1108 9 0.18045834093263835 1108 10-11 0.14941400871107646 1108 12-13 0.1350289327424079 1108 14-15 0.2016183247442953 1108 16-17 0.1603216559591587 1108 18-19 0.11359824670056184 1108 20-21 0.15544364212406236 1108 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52-53 -1.2925470848766594 2316 54-55 -1.2061186122051666 2316 56-57 -1.3813105686228297 2316 58-59 -1.2638138344962684 2316 60-61 -1.16036872779204 2316 62-63 -2.2519023701546743 2316 64-65 -1.5823877854004849 2316 66-67 -1.3007776914863811 2316 68-69 -1.220756910628431 2316 70-71 -1.1287536971659762 2316 72-73 -1.0734973346717567 2316 74-75 -1.152244292086344 2316 76-77 -0.9905508244317893 2316 78-79 -1.2327482224031776 2316 80-81 -1.102373408261606 2316 82-83 -1.0214583190794784 2316 84-85 -0.9499731573195831 2316 86-87 -1.1438739565842155 2316 88-89 -1.128651781364411 2316 90-91 -1.067755043356346 2316 92-93 -0.98800790689981 2316 94-95 -1.0167807211504858 2316 96-97 -1.0086781547585808 2316 98-99 -1.0075136800723214 2316 100 -0.933917696640524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 61.0 7 910.0 8 1071.0 9 410.0 10 218.0 11 211.0 12 289.0 13 1102.0 14 4438.0 15 11615.0 16 20137.0 17 28586.0 18 37636.0 19 49819.0 20 68569.0 21 99107.0 22 150114.0 23 236372.0 24 377502.0 25 493836.0 26 498662.0 27 440937.0 28 419288.0 29 460986.0 30 543951.0 31 661877.0 32 814854.0 33 1031197.0 34 1387546.0 35 2039889.0 36 3425546.0 37 6461222.0 38 8502180.0 39 2586617.0 40 5017.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.805693782404614 34.328996980772914 27.84924371409014 20.016065522732333 2 21.09844502771908 32.282407504015 28.172698573497335 18.446448894768583 3 24.363218677138825 27.183011396753237 27.383391984102534 21.070377942005404 4 19.9151493958286 27.62758729472825 27.16479144489824 25.292471864544915 5 20.560374998379714 32.42008438435713 26.841575174666545 20.1779654425966 6 25.787550371378547 26.877539630582458 27.09731638222199 20.237593615817005 7 20.35320590146282 27.255693548640046 32.2456403345861 20.145460215311033 8 25.21738544371334 26.907586511882727 27.367586021956225 20.507442022447705 9 25.341315463026554 26.649963586018327 27.53781603985669 20.47090491109843 10-11 20.01812792862315 29.89477402658538 29.889813196727655 20.197284848063813 12-13 20.069087413386374 27.17342024301132 29.716359125457863 23.04113321814444 14-15 20.06916031911583 32.66953044692314 26.905021850333156 20.356287383627876 16-17 22.92344554940008 27.070475732890515 26.897020041493402 23.109058676215998 18-19 20.21197564506462 29.816927444140973 26.85926492898832 23.111831981806088 20-21 22.993263881247568 29.79763482805958 26.823728107160367 20.385373183532487 22-23 22.994301817795815 27.108480679592862 29.559503258594482 20.337714244016837 24-25 20.34912160083563 29.778480826034215 26.645032795249428 23.227364777880723 26-27 20.373709587834952 27.027220926443345 32.154519569375445 20.44454991634626 28-29 20.37059958838397 27.109598567444536 32.11489119937767 20.404910644793826 30-31 20.43025753673509 27.090516707854622 32.0654060304768 20.41381972493349 32-33 20.43670726360107 27.112425689620157 32.01251859420127 20.43834845257751 34-35 20.48602555938784 27.144564155292183 31.961113574424697 20.40829671089528 36-37 20.49574017486326 27.138395150129348 31.89391120986039 20.471953465147003 38-39 20.541046703345486 27.119453801939823 31.88946992415082 20.450029570563867 40-41 20.54115010620357 27.186010860397573 31.85923366705426 20.413605366344594 42-43 20.553355865698013 27.2476818220142 31.78342044589239 20.415541866395397 44-45 20.600070538314675 27.237126085114788 31.731432059038557 20.431371317531983 46-47 20.648984284227765 27.311403733895766 31.661888020975105 20.377723960901363 48-49 20.699647054273758 27.304848743945502 31.568461048439676 20.427043153341064 50-51 20.675377940061253 27.38726408840037 31.486171630067126 20.451186341471256 52-53 20.778123789797935 27.52838678483766 31.24507443758852 20.44841498777588 54-55 21.10811722198671 27.717111532841315 30.574557770584192 20.600213474587786 56-57 21.440176388070604 28.168801594932326 29.545789215186335 20.845232801810734 58-59 21.524677190305322 28.629390502336566 28.94186459041431 20.904067716943807 60-61 21.551040917051797 28.88321767048269 28.57187526499286 20.993866147472655 62-63 21.55305312111806 29.036939044247134 28.39254446314833 21.01746337148647 64-65 21.493000931347087 29.125606264246397 28.367988800634723 21.01340400377179 66-67 21.49556597386902 28.998739569630054 28.42596130781081 21.07973314869012 68-69 21.433308705111795 28.872318537844453 28.579077231670386 21.115295525373366 70-71 21.365149628528993 28.787096066351392 28.717741332375596 21.130012972744016 72-73 21.329946402414425 28.74223525678371 28.736166181078502 21.19165215972336 74-75 21.315882257390165 28.773043177870083 28.626709178231895 21.28436538650785 76-77 21.34228433663262 28.85084551369857 28.459744747188374 21.34712540248043 78-79 21.37994771082478 28.944747698070532 28.13304482032535 21.54225977077934 80-81 21.407582893084452 29.037304622090748 27.882293108138477 21.672819376686327 82-83 21.376784149730653 29.183057195272273 27.637868997019954 21.802289657977127 84-85 21.368683110390243 29.22194717186068 27.449928331910016 21.95944138583906 86-87 21.370140937698668 29.22654683609332 27.284913918031346 22.11839830817667 88-89 21.412997751052597 29.230533675599563 27.114052279824758 22.24241629352308 90-91 21.364797155231408 29.18415673459907 26.991921860568148 22.459124249601373 92-93 21.353051508630625 29.149233445957467 26.95366148874324 22.544053556668665 94-95 21.32932099403499 29.128550285820605 26.921479061932153 22.620649658212255 96-97 21.329920025757616 29.068697135549115 26.837807526141 22.763575312552266 98-99 21.30587049324007 28.991768079426627 26.838584131632047 22.863777295701247 100 21.29909224662274 28.98727225201181 26.775651801101354 22.937983700264102 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 61970.0 1 39234.0 2 12811.0 3 8529.0 4 7698.0 5 7563.0 6 7783.5 7 7863.5 8 7972.0 9 8361.5 10 8987.0 11 11572.0 12 104886.0 13 128039.5 14 58262.5 15 60648.5 16 69993.5 17 80096.5 18 89902.0 19 100550.0 20 115182.5 21 138408.5 22 171071.5 23 205681.0 24 238114.5 25 275330.5 26 316595.5 27 364221.5 28 429301.0 29 492197.0 30 538235.5 31 592412.0 32 655147.5 33 701022.5 34 739560.5 35 797199.0 36 860856.0 37 899242.5 38 931641.5 39 960617.0 40 975309.5 41 1002718.0 42 1045583.5 43 1054873.0 44 1037399.0 45 1037421.5 46 1038052.0 47 1029921.5 48 1014528.5 49 996585.5 50 980391.5 51 962996.0 52 939169.5 53 895529.5 54 838589.0 55 774939.5 56 702669.5 57 628651.0 58 551778.0 59 466298.5 60 381402.0 61 308670.0 62 241989.0 63 180542.5 64 131627.0 65 94775.0 66 68060.0 67 50445.5 68 37319.5 69 27438.5 70 20766.0 71 16763.0 72 14157.5 73 11015.5 74 8600.5 75 7661.0 76 5795.5 77 2992.0 78 1428.5 79 962.5 80 647.5 81 447.5 82 314.0 83 214.0 84 170.5 85 134.0 86 77.5 87 49.0 88 38.5 89 27.5 90 19.5 91 14.5 92 7.0 93 3.5 94 5.0 95 5.0 96 4.0 97 2.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1069964485513016 2 0.0 3 0.0 4 0.0 5 0.009630036797627823 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.156483820825324E-5 20-21 0.011093011768734472 22-23 0.0 24-25 9.396738463364968E-4 26-27 0.004943008457194227 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.620127321269822E-6 38-39 0.0 40-41 0.007791192287986574 42-43 0.039599151986476996 44-45 0.01549813795526712 46-47 0.005067758260932004 48-49 0.001657390249659028 50-51 0.0 52-53 0.0014532542071790304 54-55 6.156483820825324E-5 56-57 8.894498993771323E-4 58-59 4.212331035301537E-5 60-61 2.948631724711076E-4 62-63 2.948631724711076E-4 64-65 7.452585677841182E-5 66-67 2.948631724711076E-4 68-69 5.119602335212638E-4 70-71 4.6659666852570875E-4 72-73 0.0012977219843371276 74-75 0.001694653178048234 76-77 0.0025905835867104455 78-79 0.004746973051320579 80-81 0.004494233189202486 82-83 0.007645380829072291 84-85 0.002452872764402511 86-87 0.0028076806477606016 88-89 0.005766033136399297 90-91 0.005694747534263425 92-93 0.005668825497123107 94-95 0.0026116452418869533 96-97 0.0057465916085440594 98-99 0.007933763492258319 100 0.2983334851932676 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0861772E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.35935873673054 #Duplication Level Percentage of deduplicated Percentage of total 1 72.8817074311783 30.14340682991544 2 13.799087244031213 11.414427991306589 3 4.711751511555507 5.846250631342697 4 2.2950168531922044 3.796817013520754 5 1.4266844689216724 2.9503377377126676 6 0.9169933955753169 2.275575528408732 7 0.66896211629304 1.9367490904332412 8 0.48064673335271163 1.5903392536297978 9 0.37957965153568285 1.4129253879324732 >10 2.092325711173091 16.16823809895383 >50 0.1989771302813533 5.717706665242725 >100 0.14737522236205106 11.0530842313541 >500 3.772577787149259E-4 0.09833207103618193 >1k 4.686057331328736E-4 0.37835446667632366 >5k 1.555567866271385E-5 0.04038451656905077 >10k+ 3.111135731900982E-5 5.177070485965404 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1472105 4.769995060555822 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80280 0.26012764270308264 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35081 0.11367137311493326 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.02487543489077685 9.720763927618932E-6 0.0 0.0 9.720763927618932E-6 2 0.02532259003144732 1.620127321269822E-5 0.0 0.0 9.720763927618932E-6 3 0.02585399179282382 1.9441527855237865E-5 0.0 0.0 2.268178249777751E-5 4 0.02586695281139398 2.9162291782856797E-5 0.0 3.2402546425396444E-6 2.268178249777751E-5 5 0.02587019306603652 2.9162291782856797E-5 0.0 9.720763927618932E-6 2.5922037140317155E-5 6 0.025879913829964137 3.888305571047573E-5 0.0 9.720763927618932E-6 2.9162291782856797E-5 7 0.025899355357819377 3.888305571047573E-5 0.0 1.9441527855237865E-5 3.564280106793609E-5 8 0.026006283761023184 4.536356499555502E-5 0.0 1.9441527855237865E-5 4.212331035301537E-5 9 0.02603544605280604 4.536356499555502E-5 0.0 1.9441527855237865E-5 4.536356499555502E-5 10-11 0.02605812783530382 5.670445624444377E-5 0.0 2.1061655176507686E-5 4.860381963809466E-5 12-13 0.026074329108516515 6.642522017206271E-5 0.0 2.7542164461586976E-5 6.156483820825325E-5 14-15 0.026087290127086674 6.966547481460235E-5 0.0 3.888305571047573E-5 7.452585677841182E-5 16-17 0.026103491400299376 7.614598409968164E-5 0.0 4.860381963809467E-5 7.776611142095146E-5 18-19 0.02614723483797366 9.720763927618932E-5 0.0 5.346420160190413E-5 8.748687534857038E-5 20-21 0.026179637384399053 1.1340891248888755E-4 0.0 6.156483820825325E-5 1.0044789391872898E-4 22-23 0.026254163241177467 1.247498037377763E-4 0.0 7.614598409968164E-5 1.1178878516761773E-4 24-25 0.026571708196146352 1.3285044034412542E-4 0.0 8.10063660634911E-5 1.2961018570158578E-4 26-27 0.02778356343245618 1.4905171355682363E-4 0.0 8.424662070603074E-5 1.45811458914284E-4 28-29 0.0318144402077755 1.6363285944825202E-4 0.0 8.74868753485704E-5 1.620127321269822E-4 30-31 0.04227560232121474 1.8145425998222008E-4 0.0 9.234725731237986E-5 1.7821400533968044E-4 32-33 0.06453453158814082 2.0089578783745793E-4 0.0 9.720763927618932E-5 2.0251591515872778E-4 34-35 0.10318104870971116 2.1061655176507688E-4 0.0 1.0530827588253844E-4 2.1061655176507688E-4 36-37 0.16333313589381712 2.203373156926958E-4 0.0 1.0854853052507808E-4 2.1547693372888635E-4 38-39 0.2505737518895545 2.3329833426285438E-4 0.0 1.101686578463479E-4 2.2357757033523545E-4 40-41 0.36577128494112393 2.430190981904733E-4 0.0 1.1340891248888755E-4 2.3167820694158456E-4 42-43 0.5122615772030199 2.511197347968224E-4 0.0 1.1826929445269701E-4 2.511197347968224E-4 44-45 0.6908239099167734 2.6732100800952067E-4 0.0 1.247498037377763E-4 2.7380151729459995E-4 46-47 0.9043550707328147 2.835222812222189E-4 0.0 1.2636993105904611E-4 2.867625358647585E-4 48-49 1.1544201026434906 2.948631724711076E-4 0.0 1.2799005838031593E-4 3.0620406371999637E-4 50-51 1.4409153175002394 3.1268457300507566E-4 0.0 1.312303130228556E-4 3.1268457300507566E-4 52-53 1.7633109336690063 3.159248276476153E-4 0.0 1.3609069498666507E-4 3.2078520961142476E-4 54-55 2.1189078190325557 3.2078520961142476E-4 0.0 1.5067184087809345E-4 3.3860661014539285E-4 56-57 2.5089048678086274 3.240254642539644E-4 0.0 1.5229196819936328E-4 3.4670724675174195E-4 58-59 2.929907913259161 3.321261008603135E-4 0.0 1.5877247748444256E-4 3.5318775603682123E-4 60-61 3.3812753849649333 3.6128839264317034E-4 0.0 1.620127321269822E-4 3.580481380006307E-4 62-63 3.862864711721673 3.7100915657078926E-4 0.0 1.6849324141206148E-4 3.758695385345987E-4 64-65 4.370212442759282 3.904506844260271E-4 0.0 1.7659387801841062E-4 3.985513210323762E-4 66-67 4.90663659883172 3.953110663898366E-4 0.0 1.8793476926729936E-4 4.1151233960253484E-4 68-69 5.467668868786925 3.985513210323762E-4 0.0 1.8955489658856918E-4 4.163727215663443E-4 70-71 6.0535457912138035 4.066519576387253E-4 0.0 1.9279515123110883E-4 4.1961297620888395E-4 72-73 6.661103257453914 4.163727215663443E-4 0.0 2.0413604247999757E-4 4.2771361281523305E-4 74-75 7.2927730786164835 4.244733581726934E-4 0.0 2.0737629712253724E-4 4.455350133492011E-4 76-77 7.946479871602966 4.325739947790425E-4 0.0 2.1385680640761653E-4 4.633564138831691E-4 78-79 8.616797830014427 4.3419412210031233E-4 0.0 2.1385680640761653E-4 4.698369231682484E-4 80-81 9.305653285235858 4.3419412210031233E-4 0.0 2.1547693372888635E-4 4.7307717781078804E-4 82-83 10.010118667197723 4.4391488602793126E-4 0.0 2.2357757033523545E-4 4.8117781441713714E-4 84-85 10.736945046447754 4.5849603191935965E-4 0.0 2.268178249777751E-4 4.8279794173840696E-4 86-87 11.477821169827838 4.682167958469786E-4 0.0 2.284379522990449E-4 4.989992149511052E-4 88 12.041068153831219 4.698369231682484E-4 0.0 2.3005807962031473E-4 5.119602335212637E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 182020 0.0 87.3606 8 GTGGTAT 185610 0.0 85.82028 6 TGGTATC 186120 0.0 85.40081 7 GTATCAA 185835 0.0 85.39017 9 AGTGGTA 186880 0.0 85.2343 5 GCAGTGG 194675 0.0 81.920395 3 AAGCAGT 191985 0.0 81.20707 1 CAGTGGT 197380 0.0 80.535805 4 AGCAGTG 204560 0.0 77.83078 2 CAACGCA 177640 0.0 45.418694 12-13 ATCAACG 180125 0.0 44.875546 10-11 TCAACGC 177155 0.0 44.769794 12-13 ACGCAGA 181180 0.0 44.62768 14-15 AACGCAG 179015 0.0 44.363937 14-15 AGAGTAC 183705 0.0 43.82966 18-19 CGCAGAG 183240 0.0 43.44877 16-17 GAGTACT 181035 0.0 43.38083 20-21 TATCAAC 185030 0.0 42.87449 10-11 AGTACTT 191770 0.0 41.68283 20-21 GTACTTT 191830 0.0 40.900486 22-23 >>END_MODULE