FastQCFastQC Report
Thu 26 May 2016
SRR1660757_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660757_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37913724
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11714663.089820456571346No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960290.25328295368716613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1514100.083.588368
GTATCAA1553250.081.411949
TGGTATC1551250.081.298897
AGTGGTA1560550.081.073895
GTGGTAT1561600.081.030776
GCAGTGG1673950.076.1298143
AAGCAGT1696150.074.965331
CAGTGGT1693350.074.693634
AGCAGTG1780500.071.585042
CAACGCA1464850.044.54131312-13
ATCAACG1488850.043.9637110-11
TCAACGC1462150.043.46544612-13
ACGCAGA1509900.043.23596214-15
AACGCAG1484650.042.88253414-15
AGAGTAC1527100.042.16752618-19
GAGTACT1498700.041.47484220-21
CGCAGAG1536200.041.415316-17
TATCAAC1567800.040.3641510-11
AGTACTT1615000.039.5873420-21
GTACTTT1623450.038.33279422-23
Produced by FastQC (version 0.11.5)