FastQCFastQC Report
Thu 26 May 2016
SRR1660757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37913724
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12744983.361574294310947No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA747050.19703946781909368No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391300.10320800984888742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1633000.083.570948
GTATCAA1672800.081.484259
AGTGGTA1684500.081.397165
TGGTATC1677150.081.387797
GTGGTAT1688350.081.101176
GCAGTGG1784700.076.8956763
AAGCAGT1767000.075.944891
CAGTGGT1816600.075.361684
AGCAGTG1894600.072.276432
CAACGCA1572400.044.64079712-13
ATCAACG1593650.044.07044210-11
TCAACGC1569750.043.40793612-13
ACGCAGA1625700.043.33389314-15
AACGCAG1595100.042.8259214-15
AGAGTAC1659750.042.2527618-19
GAGTACT1625450.041.6467320-21
CGCAGAG1654600.041.39525616-17
TATCAAC1679450.040.6143810-11
AGTACTT1745550.039.9391420-21
GTACTTT1749350.038.66612622-23
Produced by FastQC (version 0.11.5)