##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660757_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37913724 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.015785286615476 33.0 31.0 34.0 27.0 34.0 2 31.802674540754687 34.0 31.0 34.0 28.0 34.0 3 32.2971763997649 34.0 31.0 34.0 30.0 34.0 4 35.83082476941595 37.0 35.0 37.0 35.0 37.0 5 35.89219492129024 37.0 35.0 37.0 35.0 37.0 6 35.920256158429595 37.0 35.0 37.0 35.0 37.0 7 35.944598399249834 37.0 35.0 37.0 35.0 37.0 8 35.93059006812415 37.0 35.0 37.0 35.0 37.0 9 37.64067892671266 39.0 37.0 39.0 35.0 39.0 10-11 37.614639345372666 39.0 37.0 39.0 35.0 39.0 12-13 37.63431029618721 39.0 37.0 39.0 35.0 39.0 14-15 38.95516020795003 40.0 38.0 41.0 35.0 41.0 16-17 38.97103026597968 40.0 38.0 41.0 35.0 41.0 18-19 38.96499700214096 40.0 38.5 41.0 35.0 41.0 20-21 38.922502297584906 40.0 38.0 41.0 35.0 41.0 22-23 38.85476968709273 40.0 38.0 41.0 34.5 41.0 24-25 38.77812129454759 40.0 38.0 41.0 34.5 41.0 26-27 38.707595513434654 40.0 38.0 41.0 34.0 41.0 28-29 38.59502919048522 40.0 38.0 41.0 34.0 41.0 30-31 38.499038699020964 40.0 38.0 41.0 34.0 41.0 32-33 38.398744409807904 40.0 38.0 41.0 34.0 41.0 34-35 38.29554468192046 40.0 38.0 41.0 34.0 41.0 36-37 38.13642002035991 40.0 38.0 41.0 33.0 41.0 38-39 37.83203442373532 40.0 37.0 41.0 33.0 41.0 40-41 37.6935230498592 40.0 37.0 41.0 33.0 41.0 42-43 37.54747548671294 40.0 36.0 41.0 33.0 41.0 44-45 37.43962330105056 40.0 36.0 41.0 32.0 41.0 46-47 37.31545851312311 40.0 36.0 41.0 32.0 41.0 48-49 37.45816292010777 40.0 36.0 41.0 32.0 41.0 50-51 37.39383081967891 40.0 36.0 41.0 32.0 41.0 52-53 37.20278466974123 39.5 36.0 41.0 31.0 41.0 54-55 36.85936202152023 39.0 35.0 41.0 31.0 41.0 56-57 36.714662004713645 39.0 35.5 41.0 30.5 41.0 58-59 36.5634988269683 39.0 35.0 41.0 30.0 41.0 60-61 36.371438848898094 39.0 35.0 41.0 30.0 41.0 62-63 36.08700896804545 39.0 35.0 41.0 29.0 41.0 64-65 35.842936069798895 38.0 35.0 40.0 29.0 41.0 66-67 35.57820992472277 37.5 35.0 40.0 29.0 41.0 68-69 35.28205356456148 37.0 34.5 40.0 29.0 41.0 70-71 34.948844790345575 36.5 34.0 39.0 28.5 41.0 72-73 34.638179225021524 36.0 34.0 39.0 28.0 41.0 74-75 34.293944430254335 35.5 34.0 38.5 28.0 40.0 76-77 32.20461754165853 34.0 31.0 36.0 25.0 39.0 78-79 33.493872931079 35.0 33.5 37.0 27.0 39.0 80-81 33.488754230526126 35.0 34.0 36.5 27.0 39.0 82-83 33.278865101724115 35.0 34.0 36.0 27.0 38.0 84-85 33.05128977833989 35.0 34.0 36.0 27.0 37.0 86-87 32.84983462716561 35.0 33.0 35.5 27.0 37.0 88-89 32.637067345323295 35.0 33.0 35.0 26.5 36.5 90-91 32.485378605910626 35.0 33.0 35.0 26.0 36.0 92-93 32.36779395503328 35.0 33.0 35.0 26.0 36.0 94-95 32.27949565175924 35.0 33.0 35.0 26.0 36.0 96-97 32.187167699485286 35.0 33.0 35.0 25.0 35.0 98-99 32.08377396533245 35.0 33.0 35.0 25.0 35.0 100 31.90311859104107 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7860398551970995 1101 2 1.2186124492521948 1101 3 0.8008234664405478 1101 4 0.6362839699559544 1101 5 0.48428859207253083 1101 6 0.4353242895794196 1101 7 0.3864706807815921 1101 8 0.416029336406865 1101 9 0.5052082439398689 1101 10-11 0.5026413910435465 1101 12-13 0.45746055880368175 1101 14-15 0.6235755374094012 1101 16-17 0.6115580020808125 1101 18-19 0.5724390491253857 1101 20-21 0.6033645522579008 1101 22-23 0.5790970657020509 1101 24-25 0.6239081101697934 1101 26-27 0.6093236284695678 1101 28-29 0.6408980616031883 1101 30-31 0.6227083511472813 1101 32-33 0.5972827159171104 1101 34-35 0.593214571718299 1101 36-37 0.5821775033567249 1101 38-39 0.6787037376164449 1101 40-41 0.6378374719088455 1101 42-43 0.6036230101558928 1101 44-45 0.5947194749451441 1101 46-47 0.5337697814244962 1101 48-49 0.5269574329466167 1101 50-51 0.5353901105375982 1101 52-53 0.5095188540371751 1101 54-55 0.5518964650258127 1101 56-57 0.6158529871642671 1101 58-59 0.5910628648053375 1101 60-61 0.5555428243720328 1101 62-63 0.6007169516226369 1101 64-65 0.5763990956866962 1101 66-67 0.49793126356613726 1101 68-69 0.5095110497426703 1101 70-71 0.5434624516372182 1101 72-73 0.5069167330980591 1101 74-75 0.4827406660923046 1101 76-77 0.3665552028957748 1101 78-79 0.49592233756983717 1101 80-81 0.45270954173098943 1101 82-83 0.42494786741269763 1101 84-85 0.455369785227532 1101 86-87 0.433022196339202 1101 88-89 0.476494340300313 1101 90-91 0.500132644185733 1101 92-93 0.43888848640855116 1101 94-95 0.3754773176284587 1101 96-97 0.38622385214979005 1101 98-99 0.39771434596158173 1101 100 0.3360188145262235 1102 1 1.6722534409101364 1102 2 1.145816775511907 1102 3 0.7267926080002383 1102 4 0.5732345952288611 1102 5 0.4355011237291535 1102 6 0.41095576281320234 1102 7 0.3591224020286461 1102 8 0.3922036024098361 1102 9 0.49386691043188335 1102 10-11 0.4505910779704507 1102 12-13 0.41209178009439995 1102 14-15 0.583440439164697 1102 16-17 0.5541882767293203 1102 18-19 0.5333634461212782 1102 20-21 0.5302594867614374 1102 22-23 0.5192290576678218 1102 24-25 0.5691192484983247 1102 26-27 0.5740825748497898 1102 28-29 0.5684975140527584 1102 30-31 0.5756154771624793 1102 32-33 0.549661170637215 1102 34-35 0.5288714737355917 1102 36-37 0.5778672535968781 1102 38-39 0.6335033031565729 1102 40-41 0.5974119623463636 1102 42-43 0.607129410033636 1102 44-45 0.5833291282505328 1102 46-47 0.6081799250800728 1102 48-49 0.5495779844268327 1102 50-51 0.5360740337757477 1102 52-53 0.446922616590669 1102 54-55 0.4728107091966294 1102 56-57 0.5654035259818428 1102 58-59 0.5282488524920055 1102 60-61 0.49128906356511237 1102 62-63 0.5216519188744115 1102 64-65 0.5257714413333616 1102 66-67 0.4836741026194957 1102 68-69 0.4515726478563735 1102 70-71 0.4541262792505236 1102 72-73 0.39673850062929006 1102 74-75 0.4098640273707943 1102 76-77 0.34856558059204445 1102 78-79 0.467203987644055 1102 80-81 0.4465271310245029 1102 82-83 0.424073357762893 1102 84-85 0.4033300026757942 1102 86-87 0.39628249456642806 1102 88-89 0.4416296293580402 1102 90-91 0.4275576682011035 1102 92-93 0.3836609704710767 1102 94-95 0.35135167576311943 1102 96-97 0.3508404515734256 1102 98-99 0.39131235705231404 1102 100 0.3396479482263324 1103 1 1.64367892529474 1103 2 1.10084787192066 1103 3 0.6660443536163285 1103 4 0.5419411879836034 1103 5 0.408963038425199 1103 6 0.36816936226374963 1103 7 0.323925128298157 1103 8 0.3620494226906814 1103 9 0.4339689072370305 1103 10-11 0.38598890784098927 1103 12-13 0.3740649653980981 1103 14-15 0.5400989526090356 1103 16-17 0.49886293377200275 1103 18-19 0.4118660158381502 1103 20-21 0.42184089614688247 1103 22-23 0.42999555488582786 1103 24-25 0.4970942939129941 1103 26-27 0.4592217608068765 1103 28-29 0.4825921554041628 1103 30-31 0.47366808152877127 1103 32-33 0.4546343525454333 1103 34-35 0.4708791996063937 1103 36-37 0.49721833930470183 1103 38-39 0.5159070032113604 1103 40-41 0.510376152174814 1103 42-43 0.4319602036264456 1103 44-45 0.47423454421087996 1103 46-47 0.4930877769441153 1103 48-49 0.4333055978349023 1103 50-51 0.3858060409474291 1103 52-53 0.3734621358202048 1103 54-55 0.39977782758346336 1103 56-57 0.41553266591974847 1103 58-59 0.3918014166783408 1103 60-61 0.38365563610126685 1103 62-63 0.40483531760521174 1103 64-65 0.3975879498953958 1103 66-67 0.3493517214552071 1103 68-69 0.3902072099282421 1103 70-71 0.3842579516063509 1103 72-73 0.31381710734670065 1103 74-75 0.29810943031509396 1103 76-77 0.17061661850576826 1103 78-79 0.3098045808258121 1103 80-81 0.3531109653040332 1103 82-83 0.3379619542166594 1103 84-85 0.34202836949670967 1103 86-87 0.3229434245704681 1103 88-89 0.3391675692733074 1103 90-91 0.3443426148636135 1103 92-93 0.3055845157738517 1103 94-95 0.2741168637626359 1103 96-97 0.2692633915015463 1103 98-99 0.3077302307040881 1103 100 0.2185266933356722 1104 1 1.5838794190235994 1104 2 1.0667049503260024 1104 3 0.6303044725282589 1104 4 0.5187395852194712 1104 5 0.37459963697482834 1104 6 0.3532385193441385 1104 7 0.28914114437257865 1104 8 0.306177410497817 1104 9 0.4008619229249888 1104 10-11 0.37791917906775296 1104 12-13 0.3554048795276188 1104 14-15 0.5199475319713542 1104 16-17 0.476794461190174 1104 18-19 0.41034185902470455 1104 20-21 0.3913837337226056 1104 22-23 0.3943592301869572 1104 24-25 0.4555888884087125 1104 26-27 0.43746989337684994 1104 28-29 0.44176356071687195 1104 30-31 0.451133844266117 1104 32-33 0.47879939875192434 1104 34-35 0.42696160214387646 1104 36-37 0.46612417507657966 1104 38-39 0.5335178021158953 1104 40-41 0.5246340086329511 1104 42-43 0.4806015314775678 1104 44-45 0.47158611847958554 1104 46-47 0.5019539936605284 1104 48-49 0.4901592236040244 1104 50-51 0.4715729320972457 1104 52-53 0.43463711326496934 1104 54-55 0.4806955174783454 1104 56-57 0.5403527343211749 1104 58-59 0.5154020417365004 1104 60-61 0.500701086951814 1104 62-63 0.5665920654138432 1104 64-65 0.5502641949335185 1104 66-67 0.535020973878396 1104 68-69 0.5282629580729079 1104 70-71 0.5146839902548948 1104 72-73 0.5034937293053119 1104 74-75 0.452363478667543 1104 76-77 0.4202342695360173 1104 78-79 0.5001304804834987 1104 80-81 0.46669593332541837 1104 82-83 0.4381967854941138 1104 84-85 0.4535838725587027 1104 86-87 0.4474863791032888 1104 88-89 0.44407396911095987 1104 90-91 0.47797320833551993 1104 92-93 0.47162044491609123 1104 94-95 0.4239409311937763 1104 96-97 0.4039573479178813 1104 98-99 0.4603684583085723 1104 100 0.3727109872058243 1105 1 1.5791473561882263 1105 2 1.0269209873478538 1105 3 0.6156559029543658 1105 4 0.49599182320037016 1105 5 0.3347189128856556 1105 6 0.27937773748436 1105 7 0.23866120414681546 1105 8 0.25581678376055805 1105 9 0.3347094743832386 1105 10-11 0.2988784192576972 1105 12-13 0.2880356119258849 1105 14-15 0.40186016832881677 1105 16-17 0.33547953629116023 1105 18-19 0.33771458213133343 1105 20-21 0.33144879965143303 1105 22-23 0.29461258284923275 1105 24-25 0.36067503891574404 1105 26-27 0.34169192451286534 1105 28-29 0.36558382473493367 1105 30-31 0.36191868965286034 1105 32-33 0.38074517175910927 1105 34-35 0.3589883189218952 1105 36-37 0.361584474280896 1105 38-39 0.42851660139486114 1105 40-41 0.4264071014919537 1105 42-43 0.35426569700747734 1105 44-45 0.390546954995159 1105 46-47 0.4166926888861511 1105 48-49 0.41920753598225957 1105 50-51 0.3864497246133993 1105 52-53 0.3024184896156754 1105 54-55 0.3787157337799343 1105 56-57 0.36332565968339026 1105 58-59 0.37266651373380455 1105 60-61 0.34542025360810413 1105 62-63 0.38806851978581847 1105 64-65 0.3395716215070621 1105 66-67 0.35233169347944937 1105 68-69 0.3708505864544449 1105 70-71 0.36961373176794865 1105 72-73 0.3065283674647148 1105 74-75 0.24956107358644886 1105 76-77 0.24495665322007198 1105 78-79 0.37511751002074334 1105 80-81 0.3338779602885893 1105 82-83 0.31619207986245357 1105 84-85 0.34232290551959466 1105 86-87 0.30358981250493144 1105 88-89 0.3552304593652522 1105 90-91 0.3513218851670601 1105 92-93 0.30075432585866935 1105 94-95 0.27464434967412643 1105 96-97 0.31972644055108645 1105 98-99 0.3140143738183454 1105 100 0.2716175352124033 1106 1 1.43862388171598 1106 2 0.8760100971535998 1106 3 0.48216817104567156 1106 4 0.4419417625455324 1106 5 0.25071307057322656 1106 6 0.23608336352585013 1106 7 0.18078173040193235 1106 8 0.2030409810354996 1106 9 0.25333242323422667 1106 10-11 0.21576951251390852 1106 12-13 0.19643453020066914 1106 14-15 0.32240160894823333 1106 16-17 0.2632483459035697 1106 18-19 0.19130328765695737 1106 20-21 0.21852376901468773 1106 22-23 0.1849926478533419 1106 24-25 0.22892205811582045 1106 26-27 0.2264616203937706 1106 28-29 0.26351223260553525 1106 30-31 0.25408164844424874 1106 32-33 0.23287404773329712 1106 34-35 0.2583495962283777 1106 36-37 0.26692535820377117 1106 38-39 0.31425215416247454 1106 40-41 0.29476292834691975 1106 42-43 0.23469084545744323 1106 44-45 0.25380064095531907 1106 46-47 0.2989541662422326 1106 48-49 0.2830626294616394 1106 50-51 0.25998382534707787 1106 52-53 0.2086688192772712 1106 54-55 0.24281672461565762 1106 56-57 0.24259626143097535 1106 58-59 0.20126950061155213 1106 60-61 0.23448627541068134 1106 62-63 0.25175811013262717 1106 64-65 0.23195586258551515 1106 66-67 0.22606918562627953 1106 68-69 0.23880791343858476 1106 70-71 0.25503684358513823 1106 72-73 0.19826762345423532 1106 74-75 0.17471904826476248 1106 76-77 0.10457015491952149 1106 78-79 0.234544268754469 1106 80-81 0.21091791937405446 1106 82-83 0.2155332418530378 1106 84-85 0.22582162673765538 1106 86-87 0.1925544306789675 1106 88-89 0.21940546519811477 1106 90-91 0.24666944478080666 1106 92-93 0.20891672965733932 1106 94-95 0.21156887827086024 1106 96-97 0.23138203217489917 1106 98-99 0.27943718103891513 1106 100 0.19715330186750535 1107 1 1.5757279153427213 1107 2 1.0372083945277204 1107 3 0.6121994156957555 1107 4 0.5118936621201513 1107 5 0.35256745116691235 1107 6 0.34245177212562083 1107 7 0.282891572604143 1107 8 0.2915965047325386 1107 9 0.34911916923472575 1107 10-11 0.31375196893750257 1107 12-13 0.32544399813082947 1107 14-15 0.43844970856189747 1107 16-17 0.4101853219014444 1107 18-19 0.3729809075037096 1107 20-21 0.3839464573261324 1107 22-23 0.40129468392334644 1107 24-25 0.4219298768412898 1107 26-27 0.40000192769533527 1107 28-29 0.36043782849438344 1107 30-31 0.3856106244863824 1107 32-33 0.404063965149966 1107 34-35 0.44743535941282886 1107 36-37 0.4806607745866671 1107 38-39 0.47310619511576135 1107 40-41 0.46849578024330896 1107 42-43 0.44616608585227624 1107 44-45 0.4034226681991626 1107 46-47 0.45683924894708383 1107 48-49 0.4446447114736287 1107 50-51 0.42196396683042536 1107 52-53 0.38519878376241223 1107 54-55 0.4180052816650601 1107 56-57 0.3552004047484374 1107 58-59 0.32329472756998 1107 60-61 0.28051414109496875 1107 62-63 0.36719440464300135 1107 64-65 0.33296294266686743 1107 66-67 0.3362947441747153 1107 68-69 0.301827950485567 1107 70-71 0.3264599755849886 1107 72-73 0.32521756270719493 1107 74-75 0.3187000990607274 1107 76-77 0.25869446609737423 1107 78-79 0.35742666457272776 1107 80-81 0.3260211552584167 1107 82-83 0.30279491454853513 1107 84-85 0.3057583112627711 1107 86-87 0.29179355699797327 1107 88-89 0.3371110723906696 1107 90-91 0.34984591716106905 1107 92-93 0.3544617889426789 1107 94-95 0.337157943966794 1107 96-97 0.30309864380422624 1107 98-99 0.3611533935996434 1107 100 0.27855753501212277 1108 1 1.407998665369309 1108 2 0.8662693745222505 1108 3 0.45423186986629815 1108 4 0.37543677033023926 1108 5 0.22671347478937776 1108 6 0.17747760135979718 1108 7 0.12852695659375257 1108 8 0.14384569891030452 1108 9 0.16314139622119228 1108 10-11 0.13232142399306213 1108 12-13 0.1297051626128436 1108 14-15 0.1813049247753753 1108 16-17 0.13468440742118304 1108 18-19 0.11240854196079653 1108 20-21 0.12102054751636615 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-0.8976064808884381 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 66.0 7 1010.0 8 1320.0 9 480.0 10 241.0 11 178.0 12 272.0 13 945.0 14 3664.0 15 9835.0 16 18904.0 17 29175.0 18 39979.0 19 55164.0 20 75354.0 21 104928.0 22 154503.0 23 239409.0 24 376556.0 25 488476.0 26 501911.0 27 475187.0 28 486332.0 29 553790.0 30 665397.0 31 814853.0 32 1006748.0 33 1283283.0 34 1727610.0 35 2557790.0 36 4314652.0 37 8165620.0 38 1.0595797E7 39 3156725.0 40 7570.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.182925091111027 33.208205566484764 26.793396629400213 22.815472713003995 2 21.476756015842707 29.656052779199428 30.365202320932656 18.501988884025213 3 22.1873852328513 27.717063615275567 27.672559941619028 22.422991210254104 4 19.449614076422563 28.264983940907516 28.03466628601295 24.250735696656967 5 21.3034994360088 31.069853400272635 27.143001695112602 20.483645468605967 6 24.405806193029207 27.06902914627959 27.905623304110144 20.61954135658106 7 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30.676448454390815 21.083327240552787 30-31 21.14440406856367 27.091875754542077 30.60769630543283 21.15602387146143 32-33 21.17977648410375 27.11809449264335 30.5616931747459 21.140435848506993 34-35 21.225779614790678 27.158897659327796 30.514354116203407 21.10096860967812 36-37 21.23924307725614 27.150246438466453 30.41190968209823 21.19860080217918 38-39 21.271718125077875 27.118429463694994 30.438409321120762 21.17144309010637 40-41 21.27321367714353 27.17653842542133 30.427454258465385 21.12279363896975 42-43 21.29656421371621 27.215543030682483 30.342939555179722 21.144953200421586 44-45 21.34482363607118 27.201517255997317 30.33366191473375 21.11999719319775 46-47 21.387357999485467 27.27697731005328 30.24957304707473 21.086091643386528 48-49 21.41945114375011 27.273412091794153 30.15793762019087 21.149199144264866 50-51 21.41851193515045 27.331897019664964 30.10880835657294 21.140782688611647 52-53 21.483493160311273 27.4692519342551 29.91207015987305 21.13518474556058 54-55 21.730158859315868 27.588785938572315 29.39403163291605 21.287023569195764 56-57 21.951051639359935 27.936151903975965 28.69350236756505 21.419294089099047 58-59 22.02399448326285 28.27732787462268 28.26471502462895 21.43396261748552 60-61 22.080112377529034 28.441178389423253 27.951614339640052 21.527094893407657 62-63 22.096269112967555 28.554167908689394 27.818189738097978 21.531373240245074 64-65 22.05935627960523 28.647204992960724 27.76717870589091 21.526260021543138 66-67 22.06857966730077 28.555030767510463 27.77769388823885 21.598695676949916 68-69 22.024775741709355 28.442036415647532 27.880502154151433 21.652685688491687 70-71 21.985414400931635 28.413613007757306 27.934194838622517 21.666777752688542 72-73 21.953590357517353 28.386341085813765 27.906239198630693 21.75382935803819 74-75 21.94392217944093 28.399532167197805 27.846978028119157 21.809567625242106 76-77 21.938335752743107 28.462359758549272 27.71762634008732 21.881678148620303 78-79 21.978108818642717 28.50824157056051 27.468854905574926 22.044794705221847 80-81 21.96938995016018 28.58240337766067 27.29044046861328 22.157766203565867 82-83 21.951286453326883 28.701714749266205 27.075306610592868 22.271692186814036 84-85 21.92445545313491 28.68770663412222 26.938602955610687 22.449234957132173 86-87 21.914136194150167 28.680058919547175 26.862846672943363 22.54295821335929 88-89 21.934606764325505 28.67470885979222 26.728285946016072 22.662398429866197 90-91 21.900292565768677 28.5858067388342 26.64660713166335 22.867293563733774 92-93 21.875195479090493 28.51641949185374 26.665188405673625 22.943196623382143 94-95 21.848152334703904 28.50687348895876 26.64338702130603 23.0015871550313 96-97 21.84339329905364 28.412720519228124 26.606737874310966 23.137148307407273 98-99 21.79974901360283 28.319691163626786 26.66408727988589 23.216472542884492 100 21.794886718562744 28.30636957483574 26.617709105066222 23.281034601535293 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 66552.0 1 42082.0 2 13516.5 3 8654.5 4 7663.0 5 7431.0 6 7578.5 7 7727.0 8 7803.0 9 8134.0 10 8984.0 11 11748.0 12 99932.5 13 120597.5 14 52855.0 15 54200.5 16 62377.0 17 71260.0 18 80902.0 19 91879.5 20 106652.0 21 129662.5 22 161436.0 23 199063.5 24 240023.5 25 284450.0 26 332121.5 27 390892.0 28 464902.0 29 539553.0 30 605217.5 31 670077.0 32 739790.5 33 800533.5 34 851858.0 35 913244.0 36 983647.5 37 1046642.0 38 1112748.0 39 1167130.5 40 1195024.5 41 1231561.5 42 1273056.5 43 1283755.0 44 1281305.0 45 1298290.5 46 1315767.5 47 1319226.0 48 1314369.5 49 1304289.5 50 1288562.0 51 1271075.5 52 1244741.0 53 1187314.5 54 1108902.5 55 1027720.0 56 940805.5 57 841941.5 58 739007.5 59 631270.0 60 517550.5 61 418458.5 62 331966.0 63 251563.0 64 188274.0 65 139683.5 66 101610.0 67 76205.0 68 57702.0 69 43409.5 70 34017.0 71 28682.0 72 24212.0 73 18409.5 74 13282.0 75 10274.5 76 7688.0 77 4800.0 78 3046.5 79 2113.5 80 1379.5 81 1001.0 82 746.0 83 472.0 84 304.5 85 221.5 86 146.0 87 80.5 88 51.0 89 38.0 90 27.0 91 18.5 92 18.0 93 16.0 94 10.5 95 7.0 96 4.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1073542657007262 2 0.0 3 0.0 4 0.0 5 0.009384464580688513 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.0664048722831875E-5 20-21 0.011109433618285558 22-23 0.0 24-25 8.980916778314891E-4 26-27 0.00507731712136745 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.007606744196375962 42-43 0.039806166231520806 44-45 0.015341410408537025 46-47 0.004635524592625087 48-49 0.0015588022954432016 50-51 0.0 52-53 0.0012805389415189074 54-55 5.4070130383393625E-5 56-57 7.886326333968142E-4 58-59 2.3738106021977688E-5 60-61 2.492501132307657E-4 62-63 2.6243794990964223E-4 64-65 7.121431806593306E-5 66-67 2.914511906031705E-4 68-69 4.932250917899809E-4 70-71 4.299234757313737E-4 72-73 0.0011011843626861871 74-75 0.0015996845891477185 76-77 0.0023632603328546673 78-79 0.0043625363733723445 80-81 0.00413438679882778 82-83 0.0071992400429986775 84-85 0.0022379758844053407 86-87 0.0026613054417972764 88-89 0.00540833182200725 90-91 0.005479546140073183 92-93 0.005434707495365003 94-95 0.002422605597909612 96-97 0.005443938981040217 98-99 0.007660814326759354 100 0.30224411614116303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.7913724E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.00302783091903 #Duplication Level Percentage of deduplicated Percentage of total 1 58.65710000848555 22.29147404103478 2 20.227623487104434 15.374218766675627 3 8.64788814917248 9.8593780203503 4 4.102719147326924 6.236629997532379 5 2.1616123534408938 4.107390721373634 6 1.3755390163747816 3.136478851308349 7 0.933109879470239 2.48227005031691 8 0.6746436866708577 2.051080224040514 9 0.49033748920201553 1.6770878323818443 >10 2.4309755021303814 16.227288484960606 >50 0.17466348027370232 4.618695723688962 >100 0.12260745806512001 7.75886782331374 >500 7.675297058816915E-4 0.18126691208604778 >1k 3.7830933395515515E-4 0.2656050373380721 >5k 6.900652351367289E-6 0.013198825243534364 >10k+ 2.7602590389340502E-5 3.7190686883545885 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1274498 3.361574294310947 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 74705 0.19703946781909368 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39130 0.10320800984888742 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0054993278950914975 0.0 0.0 0.0 7.912702007325897E-6 2 0.005509878164434599 5.2751346715505975E-6 0.0 1.0550269343101195E-5 7.912702007325897E-6 3 0.0055283411357850264 1.0550269343101195E-5 0.0 1.0550269343101195E-5 1.3187836678876493E-5 4 0.0055283411357850264 2.3738106021977688E-5 0.0 1.3187836678876493E-5 2.3738106021977688E-5 5 0.0055283411357850264 2.3738106021977688E-5 0.0 1.5825404014651793E-5 3.1650808029303586E-5 6 0.0055283411357850264 3.692594270085418E-5 0.0 1.5825404014651793E-5 3.4288375365078885E-5 7 0.005533616270456576 3.692594270085418E-5 0.0 1.5825404014651793E-5 3.692594270085418E-5 8 0.005565267078485881 3.692594270085418E-5 0.0 1.846297135042709E-5 4.7476212043955376E-5 9 0.005573179780493206 4.483864470818008E-5 0.0 2.3738106021977688E-5 5.0113779379730675E-5 10-11 0.005573179780493206 4.879499571184302E-5 0.0 2.5056889689865337E-5 5.802648138705657E-5 12-13 0.00558241126616842 5.802648138705657E-5 0.0 2.6375673357752986E-5 5.802648138705657E-5 14-15 0.005594280319179409 6.462039972649481E-5 0.0 2.6375673357752986E-5 5.802648138705657E-5 16-17 0.005616699641533498 6.989553439804542E-5 0.0 3.0332024361415934E-5 7.385188540170837E-5 18-19 0.0056496692332306895 7.385188540170837E-5 0.0 3.1650808029303586E-5 7.912702007325896E-5 20-21 0.005691870310603094 8.044580374114662E-5 0.0 3.1650808029303586E-5 9.89087750915737E-5 22-23 0.005751215575658039 8.57209384126972E-5 0.0 3.1650808029303586E-5 1.0550269343101196E-4 24-25 0.005995190554217255 8.83585057484725E-5 0.0 3.1650808029303586E-5 1.1605296277411315E-4 26-27 0.007011972762158631 9.099607308424781E-5 0.0 3.692594270085418E-5 1.252844484493267E-4 28-29 0.010544994208429644 9.36336404200231E-5 0.0 4.220107737240478E-5 1.4242863613186612E-4 30-31 0.019425683427985077 9.89087750915737E-5 0.0 4.220107737240478E-5 1.5693525647863027E-4 32-33 0.03814713637731815 1.0550269343101195E-4 0.0 4.220107737240478E-5 1.6221039115018086E-4 34-35 0.07156907087259484 1.2132809744566374E-4 0.0 4.220107737240478E-5 1.661667421538438E-4 36-37 0.12376125331291646 1.2660323211721435E-4 0.0 4.3519861040292425E-5 1.740794441611697E-4 38-39 0.19989463446007044 1.2924079945298962E-4 0.0 4.7476212043955376E-5 1.8199214616849562E-4 40-41 0.30198167819125343 1.3187836678876493E-4 0.0 5.143256304761833E-5 1.9122363184370916E-4 42-43 0.43228014214588895 1.3451593412454023E-4 0.0 5.538891405128127E-5 1.9517998284737212E-4 44-45 0.595440057536949 1.3979106879609082E-4 0.0 5.802648138705657E-5 1.9517998284737212E-4 46-47 0.7913783409933564 1.3979106879609082E-4 0.0 6.462039972649481E-5 1.9517998284737212E-4 48-49 1.0232271037263447 1.4242863613186612E-4 0.0 6.725796706227012E-5 1.9649876651525977E-4 50-51 1.291098442347684 1.4770377080341673E-4 0.0 7.385188540170837E-5 2.136429541977992E-4 52-53 1.5907366419611009 1.5297890547496732E-4 0.0 7.912702007325896E-5 2.241932235409004E-4 54-55 1.9258975984527398 1.5429768914285497E-4 0.0 7.912702007325896E-5 2.2946835821245097E-4 56-57 2.300899009551264 1.6484795848595616E-4 0.0 8.57209384126972E-5 2.3210592554822628E-4 58-59 2.711171289847444 1.7276066049328208E-4 0.0 9.495242408791075E-5 2.3606227655188924E-4 60-61 3.15180065139473 1.7539822782905735E-4 0.0 9.758999142368606E-5 2.55844031570204E-4 62-63 3.622601145695949 1.793545788327203E-4 0.0 1.120966117704502E-4 2.769445702564064E-4 64-65 4.1243350297111405 1.8199214616849562E-4 0.0 1.1605296277411314E-4 2.8881362326739523E-4 66-67 4.6565011656465085 1.846297135042709E-4 0.0 1.1737174644200079E-4 2.993638926104964E-4 68-69 5.217750437809801 1.872672808400462E-4 0.0 1.1869053010988844E-4 3.0991416195359756E-4 70-71 5.805181522131669 1.899048481758215E-4 0.0 1.1869053010988844E-4 3.1518929662514817E-4 72-73 6.422428986400808 1.899048481758215E-4 0.0 1.2000931377777608E-4 3.27058349636137E-4 74-75 7.0599725313187385 1.951799828473721E-4 0.0 1.2660323211721435E-4 3.3760861897923824E-4 76-77 7.724563010481376 2.0177390118681035E-4 0.0 1.2924079945298962E-4 3.415649699829012E-4 78-79 8.411272129321826 2.057302521904733E-4 0.0 1.3319715045665258E-4 3.547528066617777E-4 80-81 9.121358007459252 2.0836781952624859E-4 0.0 1.3715350146031554E-4 3.6398429233699123E-4 82-83 9.849960927077488 2.0836781952624859E-4 0.0 1.477037708034167E-4 3.6925942700854184E-4 84-85 10.593777335088475 2.110053868620239E-4 0.0 1.5825404014651793E-4 3.8112848001953064E-4 86-87 11.354793319696054 2.1891808886934978E-4 0.0 1.635291748180685E-4 4.009102350378454E-4 88 11.93809924870477 2.1891808886934978E-4 0.0 1.6880430948961912E-4 4.06185369709396E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 163300 0.0 83.57094 8 GTATCAA 167280 0.0 81.48425 9 AGTGGTA 168450 0.0 81.39716 5 TGGTATC 167715 0.0 81.38779 7 GTGGTAT 168835 0.0 81.10117 6 GCAGTGG 178470 0.0 76.895676 3 AAGCAGT 176700 0.0 75.94489 1 CAGTGGT 181660 0.0 75.36168 4 AGCAGTG 189460 0.0 72.27643 2 CAACGCA 157240 0.0 44.640797 12-13 ATCAACG 159365 0.0 44.070442 10-11 TCAACGC 156975 0.0 43.407936 12-13 ACGCAGA 162570 0.0 43.333893 14-15 AACGCAG 159510 0.0 42.82592 14-15 AGAGTAC 165975 0.0 42.25276 18-19 GAGTACT 162545 0.0 41.64673 20-21 CGCAGAG 165460 0.0 41.395256 16-17 TATCAAC 167945 0.0 40.61438 10-11 AGTACTT 174555 0.0 39.93914 20-21 GTACTTT 174935 0.0 38.666126 22-23 >>END_MODULE