FastQCFastQC Report
Thu 26 May 2016
SRR1660756_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660756_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29178438
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14146664.848326699325029No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA803330.2753163140535487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1761600.086.147828
GTATCAA1782250.085.020539
AGTGGTA1786850.084.951935
TGGTATC1780700.084.8835147
GTGGTAT1787900.084.867316
AAGCAGT1873850.081.381851
GCAGTGG1889550.080.958343
CAGTGGT1889600.080.307684
AGCAGTG1975200.077.5232
CAACGCA1720800.045.16245712-13
ATCAACG1733650.044.93337210-11
ACGCAGA1748700.044.4942814-15
TCAACGC1716250.044.40366712-13
AACGCAG1737700.043.95118714-15
AGAGTAC1754400.043.80632418-19
GAGTACT1726550.043.22078320-21
CGCAGAG1769900.043.11248416-17
TATCAAC1790950.042.3352310-11
AGTACTT1805400.042.19420-21
GTACTTT1823800.040.93971622-23
Produced by FastQC (version 0.11.5)