##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660756_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29178438 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.693883819277783 33.0 31.0 34.0 30.0 34.0 2 31.84687590199311 34.0 31.0 34.0 30.0 34.0 3 31.88757461931307 34.0 31.0 34.0 30.0 34.0 4 35.305845021587515 37.0 35.0 37.0 33.0 37.0 5 35.30048160905666 37.0 35.0 37.0 33.0 37.0 6 35.30458145154994 37.0 35.0 37.0 32.0 37.0 7 35.29034206697425 37.0 35.0 37.0 32.0 37.0 8 35.28945391113808 37.0 35.0 37.0 32.0 37.0 9 36.9024671916982 39.0 37.0 39.0 33.0 39.0 10-11 36.84915465659951 39.0 37.0 39.0 33.0 39.0 12-13 36.870733176326986 39.0 37.0 39.0 33.0 39.0 14-15 38.15580266839507 40.0 38.0 41.0 33.0 41.0 16-17 38.124919452508045 40.0 38.0 41.0 33.0 41.0 18-19 38.08105567542718 40.0 38.0 41.0 33.0 41.0 20-21 38.04281384082314 40.0 38.0 41.0 33.0 41.0 22-23 37.916171009565346 40.0 37.5 41.0 32.5 41.0 24-25 37.843546559963215 40.0 38.0 41.0 32.0 41.0 26-27 37.812390025812896 40.0 38.0 41.0 32.0 41.0 28-29 37.72561421896539 40.0 38.0 41.0 32.0 41.0 30-31 37.60744079240979 40.0 37.0 41.0 31.5 41.0 32-33 37.48047368059935 40.0 37.0 41.0 31.0 41.0 34-35 37.34711063011666 40.0 37.0 41.0 31.0 41.0 36-37 37.148377922080684 39.5 37.0 41.0 30.0 41.0 38-39 36.7247150104471 39.0 35.0 41.0 30.0 41.0 40-41 36.70861611920419 39.0 35.0 41.0 30.0 41.0 42-43 36.72972727326939 39.0 35.0 41.0 30.0 41.0 44-45 36.67148601306211 39.0 35.0 41.0 30.0 41.0 46-47 36.79407240716587 40.0 35.0 41.0 30.0 41.0 48-49 36.73771404418564 40.0 35.0 41.0 30.0 41.0 50-51 35.62733606576198 38.5 34.0 40.0 28.0 40.5 52-53 35.67044017229435 38.5 35.0 39.5 28.0 40.5 54-55 35.94898189889397 39.0 35.0 41.0 26.5 41.0 56-57 35.628936065734564 39.0 35.0 41.0 25.0 41.0 58-59 35.34514637144044 39.0 35.0 41.0 24.0 41.0 60-61 35.12762619438368 39.0 34.5 41.0 23.5 41.0 62-63 34.882876269798956 38.0 34.0 41.0 23.0 41.0 64-65 34.619982056613175 37.5 34.0 40.0 23.0 41.0 66-67 34.34887554638806 37.0 34.0 40.0 23.0 41.0 68-69 34.056053308953686 36.5 33.5 40.0 22.0 41.0 70-71 33.73901512479866 36.0 33.0 39.0 22.0 41.0 72-73 33.40651005718675 35.5 33.0 39.0 22.0 41.0 74-75 33.06389360184394 35.0 33.0 38.5 21.0 40.0 76-77 32.693587418901586 35.0 32.0 37.0 20.0 39.5 78-79 32.34675096384529 35.0 32.0 37.0 20.0 39.0 80-81 32.0213860488351 35.0 32.0 36.0 20.0 39.0 82-83 31.713553429419353 35.0 32.0 36.0 19.0 37.5 84-85 31.445819735107136 35.0 31.0 35.5 19.0 37.0 86-87 31.191889898287222 35.0 31.0 35.0 18.0 37.0 88-89 30.998580475760903 35.0 31.0 35.0 18.0 36.0 90-91 30.823347192197197 35.0 31.0 35.0 17.5 36.0 92-93 30.65789381871641 35.0 31.0 35.0 17.0 36.0 94-95 30.495598941931025 34.0 30.5 35.0 16.5 35.5 96-97 30.385059268765517 34.0 30.0 35.0 15.0 35.0 98-99 30.28584333404002 34.0 30.0 35.0 15.0 35.0 100 30.171813755074893 34.0 30.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6921424295512821 1101 2 0.6743290894115468 1101 3 0.6771125435025205 1101 4 0.5696794763689894 1101 5 0.5781174278895307 1101 6 0.585514301546624 1101 7 0.5426355967784602 1101 8 0.5871893737010296 1101 9 0.7405299962854741 1101 10-11 0.7029597038160986 1101 12-13 0.6775344575394797 1101 14-15 0.8415347390643007 1101 16-17 0.8570397442541307 1101 18-19 0.8120312867667607 1101 20-21 0.7244493025398029 1101 22-23 0.7189935651418651 1101 24-25 0.6918506760129048 1101 26-27 0.7029622651836149 1101 28-29 0.6571739061956876 1101 30-31 0.7361231256600007 1101 32-33 0.7324930190792074 1101 34-35 0.6940404756918568 1101 36-37 0.7169097734240566 1101 38-39 0.7120693236423747 1101 40-41 0.7117802736976557 1101 42-43 0.6532595045234757 1101 44-45 0.5983278487884789 1101 46-47 0.6761145119212202 1101 48-49 0.6703070085217107 1101 50-51 0.6173459012236933 1101 52-53 0.6721512321702363 1101 54-55 0.7203323618742203 1101 56-57 0.6905872513900206 1101 58-59 0.7150362219337509 1101 60-61 0.6574583337461419 1101 62-63 0.6290944655560651 1101 64-65 0.57264171708254 1101 66-67 0.5304291611203027 1101 68-69 0.5440669097460145 1101 70-71 0.5550260095145774 1101 72-73 0.5007162422854847 1101 74-75 0.45112233987511985 1101 76-77 0.47339543911198945 1101 78-79 0.399348077440564 1101 80-81 0.441348282510333 1101 82-83 0.4120602321467608 1101 84-85 0.37164330165072457 1101 86-87 0.29827231944383925 1101 88-89 0.31052293542173004 1101 90-91 0.2917773555711278 1101 92-93 0.24857130256398108 1101 94-95 0.23844203099260142 1101 96-97 0.20837800827338882 1101 98-99 0.19222681996049928 1101 100 0.21661485224714028 1102 1 0.6293953249458539 1102 2 0.6280872339193415 1102 3 0.5976966732225257 1102 4 0.5436700733631028 1102 5 0.530431139015711 1102 6 0.5749233918113248 1102 7 0.5581205549691859 1102 8 0.5834769732788985 1102 9 0.689210827518977 1102 10-11 0.6338391664195271 1102 12-13 0.6589577431418121 1102 14-15 0.7981038658781117 1102 16-17 0.8057442016897127 1102 18-19 0.7757691752988904 1102 20-21 0.7242724254085786 1102 22-23 0.6958277340122052 1102 24-25 0.7470552814779978 1102 26-27 0.6880054039169394 1102 28-29 0.7125596994508854 1102 30-31 0.7183458200674266 1102 32-33 0.6698228840492604 1102 34-35 0.653013285242487 1102 36-37 0.6664098317786724 1102 38-39 0.7335641768969978 1102 40-41 0.6837528971399252 1102 42-43 0.6267081453719072 1102 44-45 0.6206800739621343 1102 46-47 0.696238802310944 1102 48-49 0.719446677660919 1102 50-51 0.6703283084604621 1102 52-53 0.7217827326520734 1102 54-55 0.7773096578069598 1102 56-57 0.7575491336264477 1102 58-59 0.7523948067716404 1102 60-61 0.7899706129423336 1102 62-63 0.7347020475682058 1102 64-65 0.6862115022814947 1102 66-67 0.6696072767211234 1102 68-69 0.6853338939892737 1102 70-71 0.6550225890108763 1102 72-73 0.6438077108404201 1102 74-75 0.5955771799950114 1102 76-77 0.5464645318031884 1102 78-79 0.5037040312105177 1102 80-81 0.48552426915669145 1102 82-83 0.5138469837694011 1102 84-85 0.45517534014894423 1102 86-87 0.42913092521686025 1102 88-89 0.3873876539233443 1102 90-91 0.31138493148405644 1102 92-93 0.3479998270592368 1102 94-95 0.3335169507385167 1102 96-97 0.27260715943789293 1102 98-99 0.2744208068633469 1102 100 0.260514734186188 1103 1 0.5605622001167845 1103 2 0.5537354127170211 1103 3 0.5470644701165845 1103 4 0.45910926706380906 1103 5 0.4637218564548746 1103 6 0.5027180482455762 1103 7 0.4883927554984453 1103 8 0.5307956136928382 1103 9 0.6012676072008887 1103 10-11 0.5752784018161421 1103 12-13 0.5778380904226879 1103 14-15 0.7203967244123746 1103 16-17 0.6879611436962492 1103 18-19 0.651012872943042 1103 20-21 0.6046286948666548 1103 22-23 0.609642680025722 1103 24-25 0.6273001685326989 1103 26-27 0.6563094644764504 1103 28-29 0.6247672853230526 1103 30-31 0.6335504676424293 1103 32-33 0.5897551960242922 1103 34-35 0.5941354685958018 1103 36-37 0.6187116009208253 1103 38-39 0.6120838718880677 1103 40-41 0.5602338689946151 1103 42-43 0.5784943776089335 1103 44-45 0.5749091651045646 1103 46-47 0.6345632588203287 1103 48-49 0.6184355409270026 1103 50-51 0.5547007697624267 1103 52-53 0.6041456251720945 1103 54-55 0.7142103246706739 1103 56-57 0.6924757988966448 1103 58-59 0.7081667207176281 1103 60-61 0.6408164892422406 1103 62-63 0.6152267561684752 1103 64-65 0.5950911621496502 1103 66-67 0.5264161496731177 1103 68-69 0.539367401499689 1103 70-71 0.5615577453322445 1103 72-73 0.5444141451405713 1103 74-75 0.5497983916876663 1103 76-77 0.5104728420118292 1103 78-79 0.4978877983841201 1103 80-81 0.45518116060424063 1103 82-83 0.41753648402518806 1103 84-85 0.38961629256313657 1103 86-87 0.32120655048202806 1103 88-89 0.3535893313243399 1103 90-91 0.35357874420290614 1103 92-93 0.3192318443225588 1103 94-95 0.3476738000881916 1103 96-97 0.34223835896185406 1103 98-99 0.27336881250904455 1103 100 0.3066402479436796 1104 1 0.5220471050611835 1104 2 0.5190953571728905 1104 3 0.4851372020559559 1104 4 0.4495589705423697 1104 5 0.45974518655901875 1104 6 0.4706600010687012 1104 7 0.4420251888222708 1104 8 0.4850380224365267 1104 9 0.5607617325556262 1104 10-11 0.5504524614783364 1104 12-13 0.5509045916710349 1104 14-15 0.6663078753055274 1104 16-17 0.6460059468838679 1104 18-19 0.6814383419902015 1104 20-21 0.6132512703299327 1104 22-23 0.5928008503651441 1104 24-25 0.6120725251325183 1104 26-27 0.6185493116569347 1104 28-29 0.6271001604116293 1104 30-31 0.6124106798385043 1104 32-33 0.6312424026771666 1104 34-35 0.646822519019814 1104 36-37 0.6160960763836556 1104 38-39 0.6726091342540101 1104 40-41 0.6391600799342143 1104 42-43 0.5796590306817961 1104 44-45 0.5613514197553613 1104 46-47 0.6010591438782242 1104 48-49 0.6241874215821426 1104 50-51 0.5805994411325557 1104 52-53 0.6265830547057689 1104 54-55 0.7035353444567605 1104 56-57 0.6753905774274642 1104 58-59 0.732477927912143 1104 60-61 0.7268086459947156 1104 62-63 0.6663113995259664 1104 64-65 0.6062078361658436 1104 66-67 0.6151666603633075 1104 68-69 0.6235463450908441 1104 70-71 0.6060908556272651 1104 72-73 0.5761903774996782 1104 74-75 0.5556225845821032 1104 76-77 0.5827215710980411 1104 78-79 0.5292929967428748 1104 80-81 0.5196841762036968 1104 82-83 0.5163109189947512 1104 84-85 0.5148616433036857 1104 86-87 0.4805445040468257 1104 88-89 0.4746776273072264 1104 90-91 0.42567416894251053 1104 92-93 0.44094919007539346 1104 94-95 0.4185377756865982 1104 96-97 0.44264809247483683 1104 98-99 0.37848284648772434 1104 100 0.36500491516649447 1105 1 0.42265320410153606 1105 2 0.4166648990528721 1105 3 0.3883203354387881 1105 4 0.36210380657929875 1105 5 0.37734657012710926 1105 6 0.41508096595715926 1105 7 0.34895859834156795 1105 8 0.43219551249735844 1105 9 0.47014373228227413 1105 10-11 0.44582300905177163 1105 12-13 0.45619335201986644 1105 14-15 0.5631768266259556 1105 16-17 0.5110379838240604 1105 18-19 0.527889370481887 1105 20-21 0.47831310607280386 1105 22-23 0.4713153475095382 1105 24-25 0.5069175406991633 1105 26-27 0.5024242301606989 1105 28-29 0.4609508162891913 1105 30-31 0.480609046648965 1105 32-33 0.46592302782377004 1105 34-35 0.44656471903530104 1105 36-37 0.4364026089750652 1105 38-39 0.4582408105103468 1105 40-41 0.45219463831569584 1105 42-43 0.4509142557593009 1105 44-45 0.43856517226855374 1105 46-47 0.469925209609535 1105 48-49 0.4637584457642916 1105 50-51 0.39919795906705957 1105 52-53 0.43126181366201877 1105 54-55 0.515374278324451 1105 56-57 0.5399891439867233 1105 58-59 0.5899217145417452 1105 60-61 0.5421238092122778 1105 62-63 0.4913274553790288 1105 64-65 0.43023253453230836 1105 66-67 0.43992183011939545 1105 68-69 0.40267853537653053 1105 70-71 0.4116944734062997 1105 72-73 0.3882602574669889 1105 74-75 0.3808724225309348 1105 76-77 0.3754690414136732 1105 78-79 0.3506493454557784 1105 80-81 0.37572856883551253 1105 82-83 0.4149070605554108 1105 84-85 0.38656409381414036 1105 86-87 0.3883858895383909 1105 88-89 0.35646814645776814 1105 90-91 0.28604934151604056 1105 92-93 0.3278249394493926 1105 94-95 0.2784353027678783 1105 96-97 0.2592564322883355 1105 98-99 0.29388547914201624 1105 100 0.3365491079473202 1106 1 0.3279698984185302 1106 2 0.3236744466609345 1106 3 0.27618832298099605 1106 4 0.31604826614551484 1106 5 0.2899030251316361 1106 6 0.35949850461606303 1106 7 0.3056566995673151 1106 8 0.3275157970407321 1106 9 0.4025394284063921 1106 10-11 0.39773922687111707 1106 12-13 0.3785862155425903 1106 14-15 0.4264434942855644 1106 16-17 0.4476165739613194 1106 18-19 0.43084245924725906 1106 20-21 0.39225026567803667 1106 22-23 0.3522804924168881 1106 24-25 0.3692607164077941 1106 26-27 0.4184820692051119 1106 28-29 0.3943525150838738 1106 30-31 0.40256367389049785 1106 32-33 0.3837725782142414 1106 34-35 0.3435138504513091 1106 36-37 0.3589496450154499 1106 38-39 0.38650022650035964 1106 40-41 0.3503750742555809 1106 42-43 0.3362977133188281 1106 44-45 0.28777711139108675 1106 46-47 0.3618411748050576 1106 48-49 0.36555922795255213 1106 50-51 0.3406774071447245 1106 52-53 0.3748580179662824 1106 54-55 0.40619732252434204 1106 56-57 0.4034276453996739 1106 58-59 0.44433467742579325 1106 60-61 0.4394553041008322 1106 62-63 0.43250129715165286 1106 64-65 0.3450536569176066 1106 66-67 0.383120165448652 1106 68-69 0.38340792096752097 1106 70-71 0.4161867625889428 1106 72-73 0.38366187638244753 1106 74-75 0.3877450035523822 1106 76-77 0.4029103789580475 1106 78-79 0.34573886498295536 1106 80-81 0.3234944369411963 1106 82-83 0.3779122640294936 1106 84-85 0.33691442967293384 1106 86-87 0.3482893824035287 1106 88-89 0.31795334601686065 1106 90-91 0.33457468663536716 1106 92-93 0.3118490421786255 1106 94-95 0.3342527501222925 1106 96-97 0.2774767744613946 1106 98-99 0.27927806695274526 1106 100 0.3594217824652155 1107 1 0.45177814553634477 1107 2 0.4622014084932111 1107 3 0.3936367152595004 1107 4 0.4179852642805102 1107 5 0.4407267152885268 1107 6 0.4459430161036906 1107 7 0.4106668113825762 1107 8 0.43481106339392994 1107 9 0.5617503930085732 1107 10-11 0.5360853234272653 1107 12-13 0.48985235596512666 1107 14-15 0.598454380190212 1107 16-17 0.6233695537339017 1107 18-19 0.5928637077747396 1107 20-21 0.5713260895255914 1107 22-23 0.5210606344181272 1107 24-25 0.5844454970751798 1107 26-27 0.5495336671761208 1107 28-29 0.5719716336082072 1107 30-31 0.5693195625918293 1107 32-33 0.5398427077021424 1107 34-35 0.5389426860273758 1107 36-37 0.5637729597025114 1107 38-39 0.6281064845596163 1107 40-41 0.577590733362328 1107 42-43 0.5626183649097101 1107 44-45 0.5327151867125721 1107 46-47 0.5759516115665164 1107 48-49 0.5426631300680356 1107 50-51 0.5109319122562468 1107 52-53 0.567071349565154 1107 54-55 0.5660893823403441 1107 56-57 0.5435837404129273 1107 58-59 0.6111596558420302 1107 60-61 0.5703548202558864 1107 62-63 0.5176288731135514 1107 64-65 0.5054652186659183 1107 66-67 0.469582044253805 1107 68-69 0.5171504117310235 1107 70-71 0.525881516176959 1107 72-73 0.5029675452688664 1107 74-75 0.5053078006746361 1107 76-77 0.5170180240751918 1107 78-79 0.502407414402434 1107 80-81 0.48878252789646837 1107 82-83 0.4522443610885283 1107 84-85 0.44383403297421964 1107 86-87 0.427666187390912 1107 88-89 0.4451350303518282 1107 90-91 0.4431159945005376 1107 92-93 0.43568793351823487 1107 94-95 0.49333546583940446 1107 96-97 0.49161631903021785 1107 98-99 0.4592586136750221 1107 100 0.43936067610775353 1108 1 0.2113008220335395 1108 2 0.16650391088712624 1108 3 0.2048212938237377 1108 4 0.20375525304746844 1108 5 0.18950448847709112 1108 6 0.23233863767020324 1108 7 0.20202212869538982 1108 8 0.21933674956031268 1108 9 0.252449601425063 1108 10-11 0.2267682781001028 1108 12-13 0.2008576630356771 1108 14-15 0.2750837767047827 1108 16-17 0.269458739831542 1108 18-19 0.26374165798264926 1108 20-21 0.24294618488269037 1108 22-23 0.20798276380865843 1108 24-25 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-1.623279458889499 2316 82-83 -1.8618589529321063 2316 84-85 -1.5670862791457516 2316 86-87 -1.4756871327770114 2316 88-89 -1.6277734509809285 2316 90-91 -1.539496924795003 2316 92-93 -1.4676679346613923 2316 94-95 -1.455134887542183 2316 96-97 -1.3604544393002307 2316 98-99 -1.4038330393955825 2316 100 -1.5005745006017683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 30.0 4 79.0 5 153.0 6 1546.0 7 18140.0 8 27520.0 9 35988.0 10 39901.0 11 39897.0 12 42767.0 13 49049.0 14 59243.0 15 74141.0 16 89674.0 17 106594.0 18 119018.0 19 133858.0 20 151463.0 21 184557.0 22 237516.0 23 331406.0 24 464113.0 25 541030.0 26 520508.0 27 458771.0 28 431261.0 29 455573.0 30 522281.0 31 623537.0 32 762704.0 33 977842.0 34 1320356.0 35 1938363.0 36 3152235.0 37 5644063.0 38 7242759.0 39 2373232.0 40 7270.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.06871364328698 35.054144316405164 26.847541803255247 21.02960023705261 2 21.205957380209068 32.06229265212269 27.79194997590157 18.93979999176667 3 24.793859820942405 26.665054430617623 26.61730084883337 21.923784899606602 4 19.713823904931314 27.342145377549514 26.60762938390243 26.336401333616738 5 20.99697230299672 32.21989107446795 26.315986447866035 20.467150174669293 6 26.30507157374223 26.529651792875274 26.609213968204877 20.55606266517762 7 20.709401922063133 26.98292828423509 31.839860653267323 20.467809140434454 8 25.709217196616212 26.552374051003003 26.890449036374054 20.84795971600673 9 25.77152005189586 26.232415182745562 27.16904174239896 20.827023022959626 10-11 20.36545000679783 29.607147908573715 29.449262481057502 20.578139603570953 12-13 20.453881865026354 26.820151509525214 29.258946236294175 23.467020389154257 14-15 20.45795768779672 32.498778721465484 26.355380983725034 20.687882607012753 16-17 23.404568469360836 26.74363514592522 26.331303272642625 23.520493112071318 18-19 20.596907894795464 29.5683254189275 26.26625866675934 23.5685080195177 20-21 23.469668086736302 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20.693143819089627 46-47 20.91423396235524 27.091273984652286 31.336618536592493 20.65787351639998 48-49 20.979604942403267 27.09497852610982 31.22926354549146 20.696152985995457 50-51 21.015498195866375 27.13140388246457 31.153725060964543 20.699372860704514 52-53 21.10886903629081 27.28435076627211 30.95240139891619 20.65437879852089 54-55 21.476952004517532 27.514867031480932 30.19773765515645 20.810443308845088 56-57 21.875131610750692 27.943640867637033 29.225924893720222 20.955302627892053 58-59 22.03850761771981 28.387533428197877 28.631604292763193 20.942354661319126 60-61 22.15376533691683 28.613009349963203 28.29810965883961 20.93511565428036 62-63 22.233692312831938 28.704777909174854 28.167430250686554 20.894099527306658 64-65 22.316975208558667 28.75584221498912 28.14193885963861 20.785243716813603 66-67 22.440004947101396 28.611759203767086 28.211499184227367 20.73673666490415 68-69 22.543354712073324 28.450701747777618 28.340445625865087 20.665497914283975 70-71 22.64087789076707 28.374473340921575 28.431293434871286 20.55335533344007 72-73 22.777756223183353 28.32415836257105 28.40793725165914 20.490148162586454 74-75 22.956462745302826 28.349285953116222 28.300608516814645 20.393642784766303 76-77 23.20314420072397 28.44875016426765 28.007971942356434 20.340133692651946 78-79 23.431144537389912 28.506675447785096 27.73490003243203 20.327279982392962 80-81 23.656902920723606 28.59212557335478 27.463327237206535 20.287644268715074 82-83 23.803039802640505 28.730243637440285 27.253073186239096 20.213643373680114 84-85 23.936730924011226 28.74404615504309 27.09684157635112 20.222381344594563 86-87 24.119509769066852 28.716388314646686 26.98470174884182 20.179400167444637 88-89 24.294728376698842 28.73160284638534 26.84502889118191 20.128639885733907 90-91 24.502458527959117 28.683997223466935 26.700034556640702 20.11350969193325 92-93 24.611844997192637 28.69012288489755 26.649850675555676 20.04818144235414 94-95 24.78815467433548 28.700527983035744 26.5654654747279 19.945851867900874 96-97 24.893298055746428 28.669059094553024 26.477996489009463 19.95964636069109 98-99 25.01305548473 28.639973723758217 26.417503326362635 19.92946746514914 100 25.09019755355027 28.71721606659835 26.293915147658108 19.898671232193273 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 52475.0 1 32817.5 2 11113.0 3 8657.5 4 8230.0 5 8432.0 6 8832.5 7 9164.5 8 9548.5 9 9929.5 10 10737.0 11 13017.5 12 80860.0 13 102901.0 14 59581.5 15 64594.0 16 73045.5 17 81951.0 18 90637.0 19 99780.5 20 111447.5 21 127896.5 22 150208.0 23 177501.5 24 206886.0 25 240618.5 26 281308.5 27 326250.5 28 374589.5 29 420668.0 30 464015.0 31 512012.5 32 565135.5 33 615802.0 34 664553.0 35 716533.0 36 769249.5 37 812035.0 38 851525.0 39 881585.0 40 898356.5 41 925748.5 42 953894.0 43 955083.5 44 948585.5 45 956669.0 46 965335.5 47 968321.0 48 968102.0 49 960615.0 50 944838.5 51 928991.0 52 909298.0 53 871688.0 54 823846.5 55 773280.5 56 715014.5 57 651867.5 58 582096.5 59 500841.0 60 412936.5 61 334979.5 62 267356.5 63 205165.5 64 155107.5 65 117079.5 66 86544.5 67 64813.5 68 49685.0 69 38680.0 70 31327.0 71 26354.5 72 22927.5 73 18570.5 74 14133.5 75 11651.0 76 9309.5 77 6422.5 78 4492.5 79 3445.5 80 2620.0 81 2065.0 82 1738.0 83 1485.0 84 1248.5 85 1018.5 86 829.5 87 703.5 88 593.5 89 492.5 90 423.0 91 368.5 92 306.0 93 265.0 94 229.5 95 191.0 96 161.5 97 126.0 98 93.5 99 72.5 100 48.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046623469015030894 2 0.014870569836534774 3 0.005377258371404254 4 1.3708753018239016E-4 5 5.312141794567619E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.427188254559754E-6 12-13 2.9131100163757906E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.03800409055481311 22-23 0.07520450546393197 24-25 0.031533559130204294 26-27 0.03584153476618591 28-29 0.04053849626906005 30-31 0.034090241568105875 32-33 0.03472427139519942 34-35 0.035774704595221994 36-37 0.03327285716939337 38-39 0.04356984428021815 40-41 0.06376455107021149 42-43 0.07198637569290035 44-45 0.07468014566098433 46-47 0.04809201918210975 48-49 0.03065448534290972 50-51 0.028116652440408223 52-53 0.028392541094900282 54-55 0.02922020705837646 56-57 0.029520086030650438 58-59 0.03730323055675564 60-61 0.05693245128474664 62-63 0.08014137014462529 64-65 0.10769425011715843 66-67 0.08915316165999017 68-69 0.052886655550238845 70-71 0.046539502902794176 72-73 0.05060757536095661 74-75 0.047985776346218395 76-77 0.051083954528340414 78-79 0.04396739811774708 80-81 0.042421736214940636 82-83 0.04170716746386493 84-85 0.049012219228459046 86-87 0.052662174719565184 88-89 0.05837015675753445 90-91 0.055419347670358506 92-93 0.051159352669940726 94-95 0.05303402464518492 96-97 0.05487785192613806 98-99 0.04952287027838845 100 0.051246745970432 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.9178438E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.890293289168376 #Duplication Level Percentage of deduplicated Percentage of total 1 72.62629263425589 34.78094454759484 2 15.67018688709069 15.008996918377072 3 4.913326444784694 7.0590193339849785 4 2.1330014378327196 4.086002577761072 5 1.1863642344192247 2.8407665567058182 6 0.7244026838933839 2.081511419466893 7 0.49666504828763003 1.66498043802815 8 0.3542072458772232 1.3570471112167035 9 0.27089611727100793 1.1675965056304967 >10 1.3816278886968438 12.361068747320184 >50 0.14306118891060138 4.8235368380169295 >100 0.09928592555300819 7.036321245263132 >500 2.546390462408833E-4 0.08790674281821098 >1k 3.9208892854019316E-4 0.4010759034088789 >5k 7.107030472923866E-6 0.018988560198259025 >10k+ 2.8428121865961285E-5 5.224236554208404 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1414666 4.848326699325029 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80333 0.2753163140535487 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0035197223374328675 3.427188254559754E-6 0.0 6.854376509119508E-6 6.854376509119508E-6 2 0.0035265767139419867 1.3708753018239016E-5 0.0 6.854376509119508E-6 6.854376509119508E-6 3 0.003540285466960226 1.713594127279877E-5 0.0 1.0281564763679262E-5 6.854376509119508E-6 4 0.0035574214082330245 2.0563129527358524E-5 0.0 1.0281564763679262E-5 1.3708753018239016E-5 5 0.0035574214082330245 2.3990317781918278E-5 0.0 1.0281564763679262E-5 1.3708753018239016E-5 6 0.0035608485964875843 2.7417506036478032E-5 0.0 1.0281564763679262E-5 1.3708753018239016E-5 7 0.003567702972996704 2.7417506036478032E-5 0.0 1.3708753018239016E-5 1.3708753018239016E-5 8 0.0035779845377603833 3.0844694291037786E-5 0.0 1.713594127279877E-5 1.713594127279877E-5 9 0.0036019748555423015 4.112625905471705E-5 0.0 1.713594127279877E-5 1.713594127279877E-5 10-11 0.00362082439094238 4.7980635563836557E-5 0.0 3.0844694291037786E-5 1.8849535400078647E-5 12-13 0.0036448147087242986 6.340298270935545E-5 0.0 3.0844694291037786E-5 3.0844694291037786E-5 14-15 0.0036688050265062167 7.197095334575483E-5 0.0 3.255828841831767E-5 3.427188254559754E-5 16-17 0.0036979361266699746 8.910689461855359E-5 0.0 3.7699070800157294E-5 4.45534473092768E-5 18-19 0.0037253536327064526 9.938845938223286E-5 6.854376509119508E-6 4.112625905471705E-5 4.45534473092768E-5 20-21 0.003787043021288528 1.1823799478231151E-4 6.854376509119508E-6 5.140782381839631E-5 4.626704143655668E-5 22-23 0.0038949994513071606 1.2851955954599077E-4 6.854376509119508E-6 5.99757944547957E-5 4.7980635563836557E-5 24-25 0.004304548447727052 1.4394190669150966E-4 6.854376509119508E-6 6.340298270935545E-5 5.312141794567619E-5 26-27 0.0057953753384605445 1.5079628320062918E-4 6.854376509119508E-6 7.539814160031459E-5 6.340298270935545E-5 28-29 0.0103295453992431 1.576506597097487E-4 6.854376509119508E-6 8.053892398215422E-5 7.197095334575483E-5 30-31 0.02120230013683392 1.713594127279877E-4 6.854376509119508E-6 8.22525181094341E-5 7.539814160031459E-5 32-33 0.043484164573854164 1.7650019510982734E-4 6.854376509119508E-6 8.22525181094341E-5 8.053892398215422E-5 34-35 0.08143513371072159 1.782137892371072E-4 6.854376509119508E-6 8.22525181094341E-5 8.567970636399384E-5 36-37 0.14002120332829332 1.9020894812806633E-4 6.854376509119508E-6 8.739330049127372E-5 9.253408287311335E-5 38-39 0.2235949025098602 1.9877691876446572E-4 6.854376509119508E-6 9.767486525495299E-5 1.0967002414591213E-4 40-41 0.3341251509076668 1.9877691876446572E-4 6.854376509119508E-6 1.0110205350951273E-4 1.2166518303687127E-4 42-43 0.4745353401028527 2.2105364241910413E-4 6.854376509119508E-6 1.0281564763679262E-4 1.319467478005505E-4 44-45 0.6437459057952314 2.3304880131006327E-4 6.854376509119508E-6 1.0281564763679262E-4 1.3708753018239016E-4 46-47 0.8460973819092029 2.4161677194646264E-4 6.854376509119508E-6 1.0967002414591213E-4 1.4222831256422978E-4 48-49 1.0827927115221179 2.5703911909198153E-4 6.854376509119508E-6 1.1481080652775175E-4 1.4394190669150966E-4 50-51 1.351602508674385 2.5875271321926143E-4 6.854376509119508E-6 1.1995158890959138E-4 1.4565550081878956E-4 52-53 1.6528403610912963 2.6903427798294073E-4 6.854376509119508E-6 1.2509237129143102E-4 1.4736909494606943E-4 54-55 1.9891983251468086 2.707478721102206E-4 6.854376509119508E-6 1.3023315367327064E-4 1.559370655824688E-4 56-57 2.3568071053015243 2.810294368738998E-4 6.854376509119508E-6 1.3708753018239016E-4 1.5936425383702855E-4 58-59 2.755507337301606 2.8445662512845956E-4 6.854376509119508E-6 1.405147184369499E-4 1.6107784796430845E-4 60-61 3.1840635197812848 2.895974075102992E-4 6.854376509119508E-6 1.405147184369499E-4 1.6793222447342794E-4 62-63 3.639701343848495 2.981653781466986E-4 6.854376509119508E-6 1.490826890733493E-4 1.7478660098254744E-4 64-65 4.12622327487167 2.998789722739785E-4 6.854376509119508E-6 1.5422347145518892E-4 1.8335457161894683E-4 66-67 4.636351678592254 3.0844694291037785E-4 6.854376509119508E-6 1.5422347145518892E-4 1.9020894812806633E-4 68-69 5.175988173184596 3.153013194194974E-4 6.854376509119508E-6 1.5765065970974867E-4 1.9706332463718585E-4 70-71 5.740326127121678 3.20442101801337E-4 6.854376509119508E-6 1.6107784796430845E-4 2.0049051289174562E-4 72-73 6.330229191843649 3.34150854819576E-4 6.854376509119508E-6 1.6793222447342794E-4 2.022041070190255E-4 74-75 6.940765643452195 3.3929163720141563E-4 6.854376509119508E-6 1.7650019510982734E-4 2.0391770114630537E-4 76-77 7.57715851684727 3.495732019650949E-4 6.854376509119508E-6 1.8678175987350658E-4 2.09058483528145E-4 78-79 8.233113780799371 3.547139843469346E-4 6.854376509119508E-6 1.9192254225534623E-4 2.1077207765542486E-4 80-81 8.911287506205781 3.6499554911061376E-4 6.854376509119508E-6 1.9534973050990597E-4 2.1248567178270474E-4 82-83 9.60846156329547 3.872722727652522E-4 6.854376509119508E-6 1.9534973050990597E-4 2.141992659099846E-4 84-85 10.322118682295468 4.0783540229261074E-4 6.854376509119508E-6 2.0563129527358524E-4 2.1762645416454438E-4 86-87 11.054513953077269 4.198305611835698E-4 6.854376509119508E-6 2.141992659099846E-4 2.2619442480094375E-4 88 11.612698390503288 4.283985318199693E-4 6.854376509119508E-6 2.22767236546384E-4 2.2962161305550353E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 176160 0.0 86.14782 8 GTATCAA 178225 0.0 85.02053 9 AGTGGTA 178685 0.0 84.95193 5 TGGTATC 178070 0.0 84.883514 7 GTGGTAT 178790 0.0 84.86731 6 AAGCAGT 187385 0.0 81.38185 1 GCAGTGG 188955 0.0 80.95834 3 CAGTGGT 188960 0.0 80.30768 4 AGCAGTG 197520 0.0 77.523 2 CAACGCA 172080 0.0 45.162457 12-13 ATCAACG 173365 0.0 44.933372 10-11 ACGCAGA 174870 0.0 44.49428 14-15 TCAACGC 171625 0.0 44.403667 12-13 AACGCAG 173770 0.0 43.951187 14-15 AGAGTAC 175440 0.0 43.806324 18-19 GAGTACT 172655 0.0 43.220783 20-21 CGCAGAG 176990 0.0 43.112484 16-17 TATCAAC 179095 0.0 42.33523 10-11 AGTACTT 180540 0.0 42.194 20-21 GTACTTT 182380 0.0 40.939716 22-23 >>END_MODULE