FastQCFastQC Report
Thu 26 May 2016
SRR1660756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29178438
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15410355.281417051865491No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA602070.20634072324227912No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293520.10059482964783789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1930500.086.71098
GTATCAA1951550.085.5959
TGGTATC1958200.085.5347067
AGTGGTA1965600.085.493455
GTGGTAT1969800.085.286276
AAGCAGT2002500.082.204441
GCAGTGG2052600.081.936193
CAGTGGT2055450.081.559634
AGCAGTG2130550.078.821492
CAACGCA1880150.045.2863312-13
ATCAACG1895800.044.9868510-11
ACGCAGA1914700.044.6005914-15
TCAACGC1875650.044.4992812-13
AACGCAG1897650.044.05269614-15
AGAGTAC1946650.043.75206818-19
GAGTACT1912100.043.32853320-21
CGCAGAG1937200.043.28918516-17
TATCAAC1966050.042.46871610-11
AGTACTT1994850.042.38883620-21
GTACTTT2004000.041.29821822-23
Produced by FastQC (version 0.11.5)