##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660756_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29178438 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99994201197473 33.0 31.0 34.0 27.0 34.0 2 31.788742426856434 34.0 31.0 34.0 28.0 34.0 3 32.294555417942526 34.0 31.0 34.0 30.0 34.0 4 35.82689741651009 37.0 35.0 37.0 35.0 37.0 5 35.88647699373078 37.0 35.0 37.0 35.0 37.0 6 35.92221314245814 37.0 35.0 37.0 35.0 37.0 7 35.91757307228029 37.0 35.0 37.0 35.0 37.0 8 35.923962207983855 37.0 35.0 37.0 35.0 37.0 9 37.637397348000604 39.0 37.0 39.0 35.0 39.0 10-11 37.576449534413044 39.0 37.0 39.0 35.0 39.0 12-13 37.638657148131095 39.0 37.0 39.0 35.0 39.0 14-15 38.96785376242553 40.0 38.0 41.0 35.0 41.0 16-17 38.983367769720914 40.0 38.0 41.0 35.0 41.0 18-19 38.97084053985344 40.0 38.0 41.0 35.0 41.0 20-21 38.90918408655048 40.0 38.0 41.0 35.0 41.0 22-23 38.80441823170932 40.0 38.0 41.0 34.5 41.0 24-25 38.75142106647381 40.0 38.0 41.0 34.0 41.0 26-27 38.71210115839648 40.0 38.0 41.0 34.0 41.0 28-29 38.612024571020555 40.0 38.0 41.0 34.0 41.0 30-31 38.51867214413602 40.0 38.0 41.0 34.0 41.0 32-33 38.41826239978987 40.0 38.0 41.0 34.0 41.0 34-35 38.31292895116593 40.0 38.0 41.0 34.0 41.0 36-37 38.131388510241706 40.0 38.0 41.0 33.0 41.0 38-39 37.7224847505545 40.0 36.0 41.0 33.0 41.0 40-41 37.576374975932566 40.0 36.0 41.0 33.0 41.0 42-43 37.420529244917084 40.0 36.0 41.0 33.0 41.0 44-45 37.308902107782465 39.5 35.0 41.0 32.0 41.0 46-47 37.179389417624066 39.0 35.0 41.0 32.0 41.0 48-49 37.306446921524724 39.5 35.0 41.0 32.0 41.0 50-51 37.21658290961291 39.0 35.0 41.0 31.5 41.0 52-53 36.95916503481098 39.0 35.0 41.0 31.0 41.0 54-55 36.4621848674696 39.0 35.0 41.0 29.5 41.0 56-57 36.158248498428875 39.0 35.0 41.0 28.0 41.0 58-59 35.96673502536359 39.0 35.0 41.0 28.0 41.0 60-61 35.77617276839837 39.0 35.0 41.0 27.5 41.0 62-63 35.49516828145496 38.5 35.0 40.5 27.0 41.0 64-65 35.25522111567452 38.0 35.0 40.0 26.0 41.0 66-67 34.99695406930282 37.0 34.0 40.0 26.0 41.0 68-69 34.70703128453963 37.0 34.0 40.0 26.0 41.0 70-71 34.37903612249566 36.0 34.0 39.0 26.0 41.0 72-73 34.06809257575748 36.0 34.0 39.0 26.0 41.0 74-75 33.72311975713025 35.0 33.5 38.5 26.0 40.0 76-77 31.640768330367788 34.0 30.5 35.5 22.5 38.5 78-79 32.91205665292981 35.0 33.0 37.0 25.0 39.0 80-81 32.89970916195034 35.0 33.0 36.5 25.0 39.0 82-83 32.67948393947613 35.0 33.0 36.0 25.0 38.0 84-85 32.43931909583371 35.0 33.0 36.0 24.5 37.0 86-87 32.22657662483509 35.0 33.0 35.0 24.0 37.0 88-89 32.00049474889643 35.0 33.0 35.0 23.5 36.0 90-91 31.82974426184157 35.0 33.0 35.0 23.0 36.0 92-93 31.69229067368171 35.0 33.0 35.0 23.0 36.0 94-95 31.582111009506406 35.0 33.0 35.0 21.5 36.0 96-97 31.465192190891095 35.0 33.0 35.0 20.0 35.0 98-99 31.33590500971985 35.0 32.0 35.0 20.0 35.0 100 31.141111254824537 35.0 32.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7406832762432778 1101 2 1.1990211978075855 1101 3 0.7627900744552178 1101 4 0.6122341633787229 1101 5 0.4729171292102592 1101 6 0.430893402438123 1101 7 0.3829721292970163 1101 8 0.3949197001245608 1101 9 0.51953512133543 1101 10-11 0.5006902979168544 1101 12-13 0.44266137090502156 1101 14-15 0.5963098521482166 1101 16-17 0.5878332744398733 1101 18-19 0.5262830462818613 1101 20-21 0.5738963401865291 1101 22-23 0.5678533726628032 1101 24-25 0.631053107941824 1101 26-27 0.5806605776908853 1101 28-29 0.6171196604314844 1101 30-31 0.5867096407098344 1101 32-33 0.5758454916636069 1101 34-35 0.5500663888682169 1101 36-37 0.5686364153063224 1101 38-39 0.6333107344231479 1101 40-41 0.6286712688167313 1101 42-43 0.604242515534672 1101 44-45 0.582955817349017 1101 46-47 0.5369367290608125 1101 48-49 0.5249252180515072 1101 50-51 0.5301193841998071 1101 52-53 0.5694818740811627 1101 54-55 0.6120676619741445 1101 56-57 0.6697657573805955 1101 58-59 0.6643888591519911 1101 60-61 0.6433598694026728 1101 62-63 0.6874367644962334 1101 64-65 0.6452499778922203 1101 66-67 0.5800632935183714 1101 68-69 0.5734052370826248 1101 70-71 0.5835972013632755 1101 72-73 0.5204085145787332 1101 74-75 0.5152885747896363 1101 76-77 0.44195050264703895 1101 78-79 0.5620297296631804 1101 80-81 0.5178735931226441 1101 82-83 0.44338170950723566 1101 84-85 0.44616015523117625 1101 86-87 0.4102852338067464 1101 88-89 0.4253173345022603 1101 90-91 0.41781922274750016 1101 92-93 0.35877973545682096 1101 94-95 0.3239779169124368 1101 96-97 0.3005176106307168 1101 98-99 0.303793613308855 1101 100 0.2088268837025602 1102 1 1.690644735820836 1102 2 1.155132599409665 1102 3 0.7103486919980639 1102 4 0.579877360499637 1102 5 0.4231142903405498 1102 6 0.4133352316212964 1102 7 0.3664042973293036 1102 8 0.3635572500350719 1102 9 0.42750164754439623 1102 10-11 0.4216553275112176 1102 12-13 0.37822079964299604 1102 14-15 0.5432150463355683 1102 16-17 0.5208420912620895 1102 18-19 0.5099397600794973 1102 20-21 0.4981648345615781 1102 22-23 0.5029677032051225 1102 24-25 0.5482564118022282 1102 26-27 0.5084710324975461 1102 28-29 0.5227771859187769 1102 30-31 0.5304587608573712 1102 32-33 0.5206248963739739 1102 34-35 0.5037527934622119 1102 36-37 0.5482960727152602 1102 38-39 0.6064505079083276 1102 40-41 0.5684532228326376 1102 42-43 0.5340467943128075 1102 44-45 0.5649785894966541 1102 46-47 0.5774542685795865 1102 48-49 0.5298803946170807 1102 50-51 0.50312584046236 1102 52-53 0.4415079315607855 1102 54-55 0.5255947320877041 1102 56-57 0.6093090962339929 1102 58-59 0.5725008185846434 1102 60-61 0.5441454148650422 1102 62-63 0.5777911130196856 1102 64-65 0.5593132511115044 1102 66-67 0.5382898645516008 1102 68-69 0.5071623529666525 1102 70-71 0.5038820782201938 1102 72-73 0.43073533258057495 1102 74-75 0.4486006955052275 1102 76-77 0.34749672988915137 1102 78-79 0.46987713167934686 1102 80-81 0.47221331804491484 1102 82-83 0.4561344146723414 1102 84-85 0.44024173597534144 1102 86-87 0.41131942928284815 1102 88-89 0.43304321385194555 1102 90-91 0.41751698209504085 1102 92-93 0.33970086226800333 1102 94-95 0.2833650648519388 1102 96-97 0.2817152995900578 1102 98-99 0.2826480676618601 1102 100 0.18192275934985958 1103 1 1.6496028729013013 1103 2 1.1097638656299118 1103 3 0.6693140755185212 1103 4 0.5497718195043007 1103 5 0.4064856306688256 1103 6 0.3619991934447029 1103 7 0.3442624643142054 1103 8 0.3552956168977701 1103 9 0.4446250195823609 1103 10-11 0.42261534109493937 1103 12-13 0.3991330864745066 1103 14-15 0.5579583054896986 1103 16-17 0.5019806140731049 1103 18-19 0.43378638210552367 1103 20-21 0.43824657696479363 1103 22-23 0.4676519991760486 1103 24-25 0.5118484919303725 1103 26-27 0.4519440908635559 1103 28-29 0.49169671072933596 1103 30-31 0.47396625698498696 1103 32-33 0.4920688618601048 1103 34-35 0.49603230563693046 1103 36-37 0.49576369444044843 1103 38-39 0.5635127205859831 1103 40-41 0.5378286629880975 1103 42-43 0.5112630597169598 1103 44-45 0.49672565598999086 1103 46-47 0.5107410677584312 1103 48-49 0.4850710736721311 1103 50-51 0.4346978543833018 1103 52-53 0.4490208881822255 1103 54-55 0.5098105706902913 1103 56-57 0.5595306211689248 1103 58-59 0.5573025502808662 1103 60-61 0.49735191187452443 1103 62-63 0.5372735804556044 1103 64-65 0.5350228736165548 1103 66-67 0.5339124855987762 1103 68-69 0.5386609533632836 1103 70-71 0.5385168416828989 1103 72-73 0.49631582054337997 1103 74-75 0.4412470192876441 1103 76-77 0.3147865721825305 1103 78-79 0.4639505077226076 1103 80-81 0.48860122403629447 1103 82-83 0.4663982960012589 1103 84-85 0.4308142049686552 1103 86-87 0.4237346142649656 1103 88-89 0.4386086030874381 1103 90-91 0.39788585633116114 1103 92-93 0.36529771497769303 1103 94-95 0.3170453955200152 1103 96-97 0.27063595413966013 1103 98-99 0.2708463628236757 1103 100 0.19030284415022436 1104 1 1.5932194539450357 1104 2 1.0810012789069354 1104 3 0.6262926469681958 1104 4 0.5359071223927785 1104 5 0.3694350910730506 1104 6 0.32688685096499626 1104 7 0.2933566652992212 1104 8 0.3055927864356818 1104 9 0.3603583007711393 1104 10-11 0.35336707398896294 1104 12-13 0.34397029059788053 1104 14-15 0.48642863390296043 1104 16-17 0.46620000368395864 1104 18-19 0.3961594476309642 1104 20-21 0.40544087685612595 1104 22-23 0.38591608438687075 1104 24-25 0.44251314964088095 1104 26-27 0.43272435042978685 1104 28-29 0.4579495909371829 1104 30-31 0.44639282241958966 1104 32-33 0.43938789085483876 1104 34-35 0.38249361635054413 1104 36-37 0.43684406647776086 1104 38-39 0.501032741119225 1104 40-41 0.5231385584150772 1104 42-43 0.4671246230055246 1104 44-45 0.4709856295796726 1104 46-47 0.4955436480887627 1104 48-49 0.4733095804106284 1104 50-51 0.45011602256862915 1104 52-53 0.4010370548862525 1104 54-55 0.47238169023409426 1104 56-57 0.5061048668696699 1104 58-59 0.45293184539278286 1104 60-61 0.47507707588716386 1104 62-63 0.567990577784812 1104 64-65 0.5247339551468286 1104 66-67 0.5112513984111544 1104 68-69 0.4887059115098751 1104 70-71 0.5029969184671756 1104 72-73 0.48715788828953777 1104 74-75 0.47131052920740046 1104 76-77 0.41402650146552133 1104 78-79 0.47814374581137287 1104 80-81 0.42584240472422863 1104 82-83 0.41219589101801546 1104 84-85 0.43814686690085836 1104 86-87 0.44166822370578274 1104 88-89 0.4376607931016707 1104 90-91 0.41528866836261 1104 92-93 0.4096736111086017 1104 94-95 0.4086255512262831 1104 96-97 0.3657752141584538 1104 98-99 0.37575322182712867 1104 100 0.30520630186846986 1105 1 1.5612817964826995 1105 2 1.0349214593177116 1105 3 0.5992879177260875 1105 4 0.49489694513346905 1105 5 0.3351637355378898 1105 6 0.2742426213982512 1105 7 0.23708460900810024 1105 8 0.29231433507407445 1105 9 0.355360094884972 1105 10-11 0.3026954780947264 1105 12-13 0.27373741053445855 1105 14-15 0.3957170336540017 1105 16-17 0.3269259078960829 1105 18-19 0.3237818632908045 1105 20-21 0.3364871346025282 1105 22-23 0.2918228740548159 1105 24-25 0.34175664329553257 1105 26-27 0.3226002757996085 1105 28-29 0.33706221567327077 1105 30-31 0.34958853154261504 1105 32-33 0.3481829792268485 1105 34-35 0.34377312745223776 1105 36-37 0.3677509556543441 1105 38-39 0.4102991575297352 1105 40-41 0.3846395044732773 1105 42-43 0.34137507957535007 1105 44-45 0.3539947610838823 1105 46-47 0.3950774380215094 1105 48-49 0.3866022803333067 1105 50-51 0.33042701502958494 1105 52-53 0.29592820615331306 1105 54-55 0.3568389507634606 1105 56-57 0.3093949626244026 1105 58-59 0.3278989093786109 1105 60-61 0.2892178946474573 1105 62-63 0.322784577155808 1105 64-65 0.2794440095694455 1105 66-67 0.2850027492408955 1105 68-69 0.3356462562433009 1105 70-71 0.3172676000330341 1105 72-73 0.25460439338753105 1105 74-75 0.2077272627196649 1105 76-77 0.22546644066090948 1105 78-79 0.3351697050867344 1105 80-81 0.3083625561645391 1105 82-83 0.30262638743541004 1105 84-85 0.30519304202820763 1105 86-87 0.27190330452373956 1105 88-89 0.287636101512561 1105 90-91 0.25293175573157 1105 92-93 0.23938089542591356 1105 94-95 0.20512093905474416 1105 96-97 0.18461327627669633 1105 98-99 0.18651361922912457 1105 100 0.1502044410569532 1106 1 1.4303741390269487 1106 2 0.8888304761154764 1106 3 0.4931610212164017 1106 4 0.4294705875215925 1106 5 0.2534930099053696 1106 6 0.23176772548466573 1106 7 0.18459131213049318 1106 8 0.1995859084182996 1106 9 0.25636075493621746 1106 10-11 0.21889195457305988 1106 12-13 0.20860596190998848 1106 14-15 0.3399926205470152 1106 16-17 0.26469388470080446 1106 18-19 0.20809971566915664 1106 20-21 0.20607889607006058 1106 22-23 0.2028106220899204 1106 24-25 0.2461789671645036 1106 26-27 0.22906610287264328 1106 28-29 0.2802327828415301 1106 30-31 0.28320579379830946 1106 32-33 0.25568445923815375 1106 34-35 0.26854298573751834 1106 36-37 0.28411765987095094 1106 38-39 0.34971240742581244 1106 40-41 0.3171377969822018 1106 42-43 0.2784987078455785 1106 44-45 0.29774768435795806 1106 46-47 0.3257340478340609 1106 48-49 0.3069029844344726 1106 50-51 0.31696780994991514 1106 52-53 0.2447893358706139 1106 54-55 0.316725544109957 1106 56-57 0.2927883118662393 1106 58-59 0.24492216316063065 1106 60-61 0.24886397521806458 1106 62-63 0.292186281097635 1106 64-65 0.2835505790408206 1106 66-67 0.2615714674265419 1106 68-69 0.27248855824768015 1106 70-71 0.2927647869281387 1106 72-73 0.26719162841679633 1106 74-75 0.2032785479431567 1106 76-77 0.13619107831419797 1106 78-79 0.2655127795880716 1106 80-81 0.2721879008830399 1106 82-83 0.2670262492298576 1106 84-85 0.2973765814153708 1106 86-87 0.2428847701926813 1106 88-89 0.26724015985424643 1106 90-91 0.2683784630212678 1106 92-93 0.23932423871427488 1106 94-95 0.2219995141731168 1106 96-97 0.22441684157692876 1106 98-99 0.2472459028946652 1106 100 0.20268300893975777 1107 1 1.5812070050096558 1107 2 1.0447263038093801 1107 3 0.6126603968926148 1107 4 0.5118060969539471 1107 5 0.37843675574416835 1107 6 0.3415565328557477 1107 7 0.2866003053030539 1107 8 0.32283365599396774 1107 9 0.3611587901164981 1107 10-11 0.3258815127241874 1107 12-13 0.2980467136578824 1107 14-15 0.4338182626867493 1107 16-17 0.3969165027423003 1107 18-19 0.36389487465402937 1107 20-21 0.3734001965736482 1107 22-23 0.38936424898997046 1107 24-25 0.40958238515347745 1107 26-27 0.3842667146438856 1107 28-29 0.3807522746105363 1107 30-31 0.4043667580771526 1107 32-33 0.40663081034899307 1107 34-35 0.43652882782849645 1107 36-37 0.5019135050264012 1107 38-39 0.5075539888721323 1107 40-41 0.49099888847515416 1107 42-43 0.4223981175771101 1107 44-45 0.4394832309699126 1107 46-47 0.48466932422878983 1107 48-49 0.46412905592656273 1107 50-51 0.45202164705328585 1107 52-53 0.41364673398388163 1107 54-55 0.4558768968611915 1107 56-57 0.3981183899851217 1107 58-59 0.3513233254021202 1107 60-61 0.31190470479540977 1107 62-63 0.3949778054592201 1107 64-65 0.35240597912523697 1107 66-67 0.339662289773095 1107 68-69 0.32053674817419875 1107 70-71 0.34309427310400054 1107 72-73 0.3422995997054983 1107 74-75 0.31156570563425845 1107 76-77 0.23881499736509326 1107 78-79 0.33435618698231195 1107 80-81 0.3283422861085725 1107 82-83 0.33360507209690127 1107 84-85 0.3117606708390568 1107 86-87 0.30371091176183285 1107 88-89 0.3172218360575698 1107 90-91 0.33798390185858196 1107 92-93 0.35774636202506116 1107 94-95 0.33942222091485874 1107 96-97 0.3108754875754087 1107 98-99 0.34755257585708677 1107 100 0.24346957101775146 1108 1 1.4033873453355206 1108 2 0.8861649168850398 1108 3 0.45612107402700275 1108 4 0.38249155894921216 1108 5 0.2094734896322663 1108 6 0.153972389355566 1108 7 0.1161327040774367 1108 8 0.12905181870867466 1108 9 0.14813306329313036 1108 10-11 0.12075797659863241 1108 12-13 0.11127673316722309 1108 14-15 0.16317105896307282 1108 16-17 0.15266927931744334 1108 18-19 0.14045957338668558 1108 20-21 0.11781617448301773 1108 22-23 0.0990234267504988 1108 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Count 6 62.0 7 880.0 8 1037.0 9 421.0 10 197.0 11 191.0 12 247.0 13 909.0 14 3683.0 15 10118.0 16 19152.0 17 27908.0 18 36774.0 19 49884.0 20 69341.0 21 101580.0 22 157566.0 23 255547.0 24 412815.0 25 516926.0 26 501129.0 27 433815.0 28 407895.0 29 446462.0 30 523243.0 31 636237.0 32 784383.0 33 993725.0 34 1330505.0 35 1954631.0 36 3283180.0 37 6088327.0 38 7808915.0 39 2315277.0 40 5476.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.64038819315133 34.52740301175067 27.1680450819824 20.664163713115606 2 21.353027876269458 32.30941286164804 27.73131995619505 18.60623930588745 3 25.043804606675657 26.718383622865627 26.720114352934175 21.51769741752454 4 19.978660269614156 27.328728151931912 26.614457566234357 26.078154012219574 5 20.927640319115536 32.4911741439091 26.247043766649163 20.3341417703262 6 26.5529703817593 26.3941921771138 26.620023319959756 20.432814121167144 7 20.563657314349726 26.85630395979387 32.249724951006634 20.33031377484977 8 25.953894447673996 26.36430024115753 26.900956795562532 20.78084851560594 9 26.056463337756462 26.030875950247918 27.185276333160807 20.72738437883481 10-11 20.236566467334544 29.640861858335256 29.638097831007954 20.484473843322252 12-13 20.327741670064725 26.686104650290055 29.415508808250806 23.570644871394418 14-15 20.330008755095115 32.731094104489074 26.322159534379463 20.61673760603635 16-17 23.490739977239357 26.56573151722515 26.285839906851766 23.657688598683727 18-19 20.487746482061493 29.60666491274467 26.242820613654615 23.662767991539226 20-21 23.558216912669792 29.594945087273274 26.212569824163662 20.63426817589327 22-23 23.522707075683762 26.62273079868086 29.260510792250084 20.594051333385288 24-25 20.601089691816735 29.56907526513497 26.091038714644014 23.73879632840428 26-27 20.641783358530454 26.53382343941828 32.15456715769985 20.669826044351417 28-29 20.64790788321157 26.61343969132275 32.134242415580985 20.604410009884695 30-31 20.658607564942304 26.604069758634786 32.08523362354078 20.65208905288213 32-33 20.67038031080314 26.643422904327895 32.02910025596813 20.657096528900837 34-35 20.70473580525455 26.680391527469705 31.991894151427847 20.6229785158479 36-37 20.745475147529746 26.644216298422425 31.91791027192597 20.692398282121857 38-39 20.768328996912036 26.641522071880612 31.901164483170756 20.688984448036596 40-41 20.774571427538298 26.70196603912487 31.89848023823349 20.62498229510334 42-43 20.79201793593596 26.7364655279481 31.827927607238177 20.643588928877758 44-45 20.83772156901868 26.753999319362514 31.78168301594559 20.626596095673218 46-47 20.863967267990553 26.843615804275867 31.690437962473677 20.601978965259907 48-49 20.891209664684666 26.86752588188714 31.58729189038344 20.653972563044753 50-51 20.913054016119712 26.921351992865418 31.50626843013324 20.659325560881634 52-53 20.99984411215446 27.06406294722757 31.270101062516954 20.665991878101018 54-55 21.335817086865074 27.3124312751906 30.49305259639072 20.858699041553606 56-57 21.738448716717336 27.790116233190897 29.354189887843486 21.117245162248285 58-59 21.84338910626788 28.296890888234906 28.67883912876738 21.18088087672983 60-61 21.862742625080404 28.536902975192017 28.325453404633684 21.274900995093898 62-63 21.86074923094974 28.66642314143809 28.151754042238046 21.32107358537412 64-65 21.81209621803513 28.75589294724431 28.126768698131077 21.305242136589484 66-67 21.800269939851297 28.6476151908785 28.17214282698351 21.379972042286692 68-69 21.757291868621905 28.524854558812546 28.30028004726391 21.417573525301638 70-71 21.727289001812938 28.46891579254572 28.38932071930751 21.41447448633383 72-73 21.694333019918744 28.41453898006933 28.424548209089146 21.466579790922783 74-75 21.710986287874974 28.44875579426836 28.333393081602793 21.506864836253868 76-77 21.740963653088727 28.50619582338853 28.18538939985715 21.56745112366559 78-79 21.81928916194921 28.614910798225047 27.854504781316585 21.711295258509153 80-81 21.86496513619966 28.744947229056567 27.587345776538026 21.802741858205746 82-83 21.862926445235466 28.90038557229469 27.339833245479312 21.89685473699053 84-85 21.87897070813939 28.933153812856588 27.148256564167262 22.039618914836762 86-87 21.905125572401673 28.948804047843602 26.99765208661114 22.148418293143582 88-89 22.002737420709728 28.977936890849215 26.814070751678166 22.205254936762888 90-91 22.03266020446225 28.94330245564522 26.67432996520412 22.349707374688414 92-93 22.049238357344528 28.917694075218513 26.626715069497326 22.40635249793963 94-95 22.080572479424955 28.917243742361983 26.591843395161206 22.410340383051853 96-97 22.107003238548177 28.839696432185175 26.524254700441276 22.529045628825372 98-99 22.11239320432823 28.783259304283337 26.495929433343164 22.608418058045267 100 22.129239459467467 28.799262658313673 26.441965089979963 22.629532792238898 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 45196.0 1 28895.5 2 9632.0 3 6299.5 4 5851.5 5 5817.5 6 5962.5 7 6110.0 8 6227.0 9 6552.5 10 7384.0 11 9958.5 12 89839.0 13 112231.0 14 56376.5 15 60461.5 16 69525.5 17 79059.0 18 88413.5 19 98421.0 20 111023.5 21 128802.0 22 152354.0 23 180354.0 24 210857.5 25 245719.5 26 287590.0 27 333404.0 28 382344.0 29 430026.5 30 473228.0 31 520468.5 32 574548.0 33 624974.0 34 671850.5 35 721395.5 36 773054.0 37 815210.5 38 854686.0 39 885256.0 40 902885.5 41 930818.5 42 960714.0 43 964641.0 44 957094.0 45 963078.5 46 967986.5 47 964645.5 48 959683.5 49 953667.0 50 943605.0 51 930854.5 52 914003.5 53 877073.5 54 826008.5 55 774854.0 56 716398.0 57 650136.5 58 578169.5 59 495567.0 60 405964.5 61 326777.0 62 258622.5 63 196723.0 64 147441.0 65 109974.0 66 79964.5 67 59562.0 68 45242.5 69 34421.0 70 27387.5 71 23230.5 72 20236.0 73 15896.5 74 11677.5 75 9429.0 76 7301.0 77 4616.5 78 2850.5 79 1950.5 80 1285.0 81 946.0 82 693.0 83 472.5 84 347.0 85 264.5 86 181.0 87 117.5 88 73.5 89 43.0 90 28.0 91 20.5 92 16.0 93 11.5 94 8.5 95 7.0 96 4.0 97 2.0 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10444013486945396 2 0.0 3 0.0 4 0.0 5 0.009589272736258191 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.368454747303472E-5 20-21 0.010615715618498838 22-23 0.0 24-25 9.596127112767311E-4 26-27 0.005104796905166754 28-29 0.0 30-31 0.0 32-33 1.713594127279877E-6 34-35 0.0 36-37 1.713594127279877E-6 38-39 0.0 40-41 0.007496974306849462 42-43 0.0387118049293797 44-45 0.014796885289061737 46-47 0.004702102285255983 48-49 0.001561084249951968 50-51 0.0 52-53 0.0014034335902422194 54-55 6.683017096391521E-5 56-57 8.242387752216209E-4 58-59 2.7417506036478032E-5 60-61 2.9473818989213886E-4 62-63 2.690342779829407E-4 64-65 7.025735921847497E-5 66-67 2.9473818989213886E-4 68-69 5.106510499294034E-4 70-71 4.266849376926894E-4 72-73 0.00125606449529615 74-75 0.0015970697266248454 76-77 0.0024607211667739036 78-79 0.004551306002055353 80-81 0.004299407665345211 82-83 0.007531246189395059 84-85 0.0024264492842283056 86-87 0.002839425468902756 88-89 0.005834788003387981 90-91 0.0058502103505335004 92-93 0.005593171231441519 94-95 0.0027263282565022843 96-97 0.005584603260805119 98-99 0.007810562032141679 100 0.2978397952625154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.9178438E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.67115672001606 #Duplication Level Percentage of deduplicated Percentage of total 1 71.46233973756057 33.35230057470721 2 16.259772164807302 15.177247498709528 3 5.2127790583486 7.298592851370158 4 2.218641347813925 4.141862321973255 5 1.2183138358191425 2.843005798283956 6 0.7423113618623215 2.078671794271498 7 0.5285858860153125 1.726880030834644 8 0.36571843240670204 1.3654801819401434 9 0.26814962633909434 1.1263367911757056 >10 1.4708025894045604 12.75673875056972 >50 0.15032570813961368 4.944014016810271 >100 0.10157026832135609 6.993006782793879 >500 2.3783873199981938E-4 0.07725982642331021 >1k 4.0850060777777625E-4 0.3940368124300935 >5k 7.2739702735358316E-6 0.02037926093497412 >10k+ 3.636985136132881E-5 5.704186706771694 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1541035 5.281417051865491 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60207 0.20634072324227912 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 29352 0.10059482964783789 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0034306154428143136 6.854376509119508E-6 0.0 0.0 1.3708753018239016E-5 2 0.0034340426310688734 2.3990317781918278E-5 0.0 0.0 1.3708753018239016E-5 3 0.003454605760596232 3.427188254559754E-5 0.0 0.0 1.3708753018239016E-5 4 0.003458032948850792 3.427188254559754E-5 0.0 0.0 1.713594127279877E-5 5 0.003468314513614471 3.7699070800157294E-5 0.0 0.0 2.0563129527358524E-5 6 0.0034785960783781504 4.45534473092768E-5 0.0 0.0 3.0844694291037786E-5 7 0.00348202326663271 4.7980635563836557E-5 0.0 0.0 3.0844694291037786E-5 8 0.00348202326663271 5.4835012072956065E-5 0.0 3.427188254559754E-6 3.7699070800157294E-5 9 0.003495732019650949 5.4835012072956065E-5 0.0 3.427188254559754E-6 3.7699070800157294E-5 10-11 0.0035248631198147072 7.711173572759446E-5 0.0 8.567970636399385E-6 4.45534473092768E-5 12-13 0.003541999061087506 7.882532985487434E-5 0.0 1.1995158890959139E-5 4.7980635563836557E-5 14-15 0.0035745573495058235 8.22525181094341E-5 0.0 2.913110016375791E-5 6.340298270935545E-5 16-17 0.0036054020437968613 9.082048874583348E-5 0.0 3.5985476672877414E-5 7.539814160031458E-5 18-19 0.0036431011145970187 9.253408287311335E-5 0.0 4.112625905471705E-5 9.253408287311337E-5 20-21 0.0037047905031790942 1.045292417640725E-4 0.0 4.45534473092768E-5 9.596127112767311E-5 22-23 0.003802465368434047 1.1481080652775175E-4 0.0 4.45534473092768E-5 9.596127112767311E-5 24-25 0.004225723117872176 1.2509237129143102E-4 0.0 4.7980635563836557E-5 1.0281564763679261E-4 26-27 0.005764530644169506 1.3880112430967004E-4 0.0 5.140782381839631E-5 1.0967002414591213E-4 28-29 0.010420365887988933 1.490826890733493E-4 0.0 5.140782381839631E-5 1.2337877716415115E-4 30-31 0.021521028644507972 1.6793222447342794E-4 0.0 5.140782381839631E-5 1.353739360551103E-4 32-33 0.04413190315396595 1.713594127279877E-4 0.0 5.140782381839631E-5 1.490826890733493E-4 34-35 0.08252497957567159 1.782137892371072E-4 0.0 5.140782381839631E-5 1.5936425383702855E-4 36-37 0.1418701713916283 1.9706332463718587E-4 0.0 6.168938858207557E-5 1.6107784796430845E-4 38-39 0.2265662747265635 2.09058483528145E-4 0.0 6.854376509119508E-5 1.6964581860070784E-4 40-41 0.33799616004119204 2.1248567178270474E-4 0.0 8.22525181094341E-5 1.7478660098254746E-4 42-43 0.47968640404945595 2.1934004829182426E-4 0.0 8.739330049127372E-5 1.782137892371072E-4 44-45 0.6498360193235841 2.3476239543734315E-4 0.0 9.938845938223286E-5 1.7992738336438708E-4 46-47 0.853291050055524 2.381895836919029E-4 0.0 1.0624283589135237E-4 1.8678175987350658E-4 48-49 1.091624575654118 2.3990317781918277E-4 0.0 1.11383618273192E-4 1.9534973050990597E-4 50-51 1.3625403799888123 2.467575543283023E-4 0.0 1.1995158890959138E-4 2.141992659099846E-4 52-53 1.666093298071679 2.5532552496470163E-4 0.0 1.2337877716415115E-4 2.159128600372645E-4 54-55 2.0054226343438946 2.707478721102206E-4 0.0 1.2851955954599077E-4 2.2619442480094375E-4 56-57 2.3766179670070073 2.724614662375004E-4 0.0 1.3880112430967004E-4 2.3647598956462302E-4 58-59 2.7787213969438667 2.827430310011797E-4 0.0 1.405147184369499E-4 2.381895836919029E-4 60-61 3.210757889096051 2.895974075102992E-4 0.0 1.4736909494606943E-4 2.450439602010224E-4 62-63 3.6700113967718218 2.981653781466986E-4 0.0 1.5079628320062918E-4 2.6046630734654133E-4 64-65 4.159847418837156 3.050197546558181E-4 0.0 1.559370655824688E-4 2.6732068385566083E-4 66-67 4.67452370137154 3.1530131941949734E-4 0.0 1.5765065970974867E-4 2.707478721102206E-4 68-69 5.218953118737884 3.221556959286169E-4 0.0 1.6621863034614807E-4 2.741750603647803E-4 70-71 5.788849972023862 3.272964783104565E-4 0.0 1.713594127279877E-4 2.758886544920602E-4 72-73 6.385544010272243 3.358644489468559E-4 0.0 1.7992738336438708E-4 2.878838133830193E-4 74-75 7.003661402299876 3.3929163720141563E-4 6.854376509119508E-6 1.850681657462267E-4 3.050197546558181E-4 76-77 7.648774756208677 3.4957320196509487E-4 6.854376509119508E-6 1.8849535400078648E-4 3.1701491354677725E-4 78-79 8.313217451873196 3.6842273736517356E-4 6.854376509119508E-6 1.9192254225534623E-4 3.221556959286169E-4 80-81 8.999878608992024 3.7699070800157296E-4 6.854376509119508E-6 1.9534973050990597E-4 3.2558288418317664E-4 82-83 9.705041441903091 3.9069946101981195E-4 6.854376509119508E-6 1.9534973050990597E-4 3.34150854819576E-4 84-85 10.428565435887966 3.9241305514709185E-4 6.854376509119508E-6 1.9877691876446572E-4 3.3929163720141563E-4 86-87 11.171072625614846 4.0098102578349124E-4 6.854376509119508E-6 1.9877691876446572E-4 3.495732019650949E-4 88 11.737228702920971 4.112625905471705E-4 6.854376509119508E-6 1.9877691876446572E-4 3.5300039021965467E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 193050 0.0 86.7109 8 GTATCAA 195155 0.0 85.595 9 TGGTATC 195820 0.0 85.534706 7 AGTGGTA 196560 0.0 85.49345 5 GTGGTAT 196980 0.0 85.28627 6 AAGCAGT 200250 0.0 82.20444 1 GCAGTGG 205260 0.0 81.93619 3 CAGTGGT 205545 0.0 81.55963 4 AGCAGTG 213055 0.0 78.82149 2 CAACGCA 188015 0.0 45.28633 12-13 ATCAACG 189580 0.0 44.98685 10-11 ACGCAGA 191470 0.0 44.60059 14-15 TCAACGC 187565 0.0 44.49928 12-13 AACGCAG 189765 0.0 44.052696 14-15 AGAGTAC 194665 0.0 43.752068 18-19 GAGTACT 191210 0.0 43.328533 20-21 CGCAGAG 193720 0.0 43.289185 16-17 TATCAAC 196605 0.0 42.468716 10-11 AGTACTT 199485 0.0 42.388836 20-21 GTACTTT 200400 0.0 41.298218 22-23 >>END_MODULE