FastQCFastQC Report
Thu 26 May 2016
SRR1660755_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660755_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30024056
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11380153.7903439828382948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA897790.29902355631097943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1433500.085.855098
AGTGGTA1464200.084.248145
TGGTATC1464500.083.835677
GTATCAA1471150.083.590829
GTGGTAT1476150.083.479516
GCAGTGG1571850.079.0504153
AAGCAGT1585850.078.206261
CAGTGGT1598800.077.167194
AGCAGTG1660700.074.865482
CAACGCA1399750.044.9743212-13
ATCAACG1416250.044.5614610-11
TCAACGC1399800.044.23281512-13
ACGCAGA1430500.044.02982314-15
AACGCAG1417000.043.71809814-15
AGAGTAC1438300.043.1675218-19
GAGTACT1411100.042.91981520-21
CGCAGAG1444850.042.80890716-17
TATCAAC1464850.041.994410-11
AGTACTT1523500.040.51419420-21
GTACTTT1520350.039.8745522-23
Produced by FastQC (version 0.11.5)