##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660755_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30024056 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.705826954226303 33.0 31.0 34.0 30.0 34.0 2 31.861171155556065 34.0 31.0 34.0 30.0 34.0 3 31.903236524738695 34.0 31.0 34.0 30.0 34.0 4 35.32577470545618 37.0 35.0 37.0 33.0 37.0 5 35.32231128265948 37.0 35.0 37.0 33.0 37.0 6 35.32568194650317 37.0 35.0 37.0 32.0 37.0 7 35.32833538546557 37.0 35.0 37.0 32.0 37.0 8 35.31367913782202 37.0 35.0 37.0 32.0 37.0 9 36.92660791733135 39.0 37.0 39.0 33.0 39.0 10-11 36.89277423077016 39.0 37.0 39.0 33.0 39.0 12-13 36.89041428979482 39.0 37.0 39.0 33.0 39.0 14-15 38.169257861096455 40.0 38.0 41.0 33.0 41.0 16-17 38.13988468113702 40.0 38.0 41.0 33.0 41.0 18-19 38.09917156096431 40.0 38.0 41.0 33.0 41.0 20-21 38.07173744280253 40.0 38.0 41.0 33.0 41.0 22-23 37.96929155740983 40.0 38.0 41.0 32.5 41.0 24-25 37.88037512320121 40.0 38.0 41.0 32.0 41.0 26-27 37.828200626857345 40.0 38.0 41.0 32.0 41.0 28-29 37.730380265744245 40.0 38.0 41.0 32.0 41.0 30-31 37.60978726525157 40.0 37.0 41.0 31.5 41.0 32-33 37.481427942313985 40.0 37.0 41.0 31.0 41.0 34-35 37.3467302352487 40.0 37.0 41.0 31.0 41.0 36-37 37.158440485189615 40.0 37.0 41.0 30.0 41.0 38-39 36.788477945817846 39.0 35.0 41.0 30.0 41.0 40-41 36.77760558067171 39.0 35.0 41.0 30.0 41.0 42-43 36.80940309996757 40.0 35.0 41.0 30.0 41.0 44-45 36.75206542713616 40.0 35.0 41.0 30.0 41.0 46-47 36.88200030335675 40.0 35.0 41.0 30.0 41.0 48-49 36.82919003348515 40.0 35.0 41.0 30.0 41.0 50-51 35.72683422586209 38.5 34.0 40.0 28.5 40.5 52-53 35.80219706158289 38.5 35.0 40.0 28.0 40.5 54-55 36.175201911427294 39.0 35.0 41.0 28.0 41.0 56-57 35.93651114293152 39.0 35.0 41.0 26.5 41.0 58-59 35.66156199881854 39.0 35.0 41.0 26.0 41.0 60-61 35.43929802489044 39.0 35.0 41.0 26.0 41.0 62-63 35.188613490462444 38.0 34.0 41.0 26.0 41.0 64-65 34.91395762784349 37.5 34.0 40.0 25.0 41.0 66-67 34.627000529175675 37.0 34.0 40.0 25.0 41.0 68-69 34.32477142661871 36.5 34.0 39.5 25.0 41.0 70-71 33.997331539749325 36.0 33.0 39.0 24.0 41.0 72-73 33.6576951328628 35.5 33.0 39.0 24.0 41.0 74-75 33.30809306710592 35.0 33.0 38.5 24.0 40.0 76-77 32.9300483419029 35.0 33.0 37.0 23.0 39.5 78-79 32.57842731175295 35.0 32.0 37.0 22.5 39.0 80-81 32.25387853992812 35.0 32.0 36.0 21.5 39.0 82-83 31.949764199080896 35.0 32.0 36.0 20.0 37.0 84-85 31.689201635515204 35.0 32.0 35.5 20.0 37.0 86-87 31.440429967223615 35.0 31.5 35.0 20.0 37.0 88-89 31.25092236038995 35.0 31.0 35.0 20.0 36.0 90-91 31.081593656100296 35.0 31.0 35.0 19.0 36.0 92-93 30.923585424301102 35.0 31.0 35.0 19.0 36.0 94-95 30.765045751979677 34.5 31.0 35.0 18.0 35.5 96-97 30.6532181061746 34.0 31.0 35.0 18.0 35.0 98-99 30.55492947055521 34.0 30.5 35.0 18.0 35.0 100 30.438066795505577 34.0 30.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7165865329312204 1101 2 0.6920196693932219 1101 3 0.7018327550002468 1101 4 0.5931591247252257 1101 5 0.6011186212031134 1101 6 0.6238912803294454 1101 7 0.5660522211258581 1101 8 0.6181030898376108 1101 9 0.7460800210319434 1101 10-11 0.7264113948805431 1101 12-13 0.7125126168291089 1101 14-15 0.9130343711340672 1101 16-17 0.8878191371073356 1101 18-19 0.8430179600819159 1101 20-21 0.7953857108394047 1101 22-23 0.7693861670181121 1101 24-25 0.711161069180207 1101 26-27 0.7592173061556835 1101 28-29 0.7195854011960563 1101 30-31 0.7679441592446707 1101 32-33 0.7649732115187717 1101 34-35 0.7525132992520511 1101 36-37 0.7423625669443155 1101 38-39 0.7842128680953806 1101 40-41 0.7792894185160861 1101 42-43 0.69618743339052 1101 44-45 0.6502611049014178 1101 46-47 0.7179745085531763 1101 48-49 0.7202098754601423 1101 50-51 0.7410891285319607 1101 52-53 0.769436056342343 1101 54-55 0.7945117560075659 1101 56-57 0.7799245533148564 1101 58-59 0.755516803038951 1101 60-61 0.7399422980795194 1101 62-63 0.7125411873261385 1101 64-65 0.6434534239377498 1101 66-67 0.6368416718961072 1101 68-69 0.6432407026793641 1101 70-71 0.6254873744720513 1101 72-73 0.567005509437017 1101 74-75 0.5140105160652624 1101 76-77 0.5489058670561633 1101 78-79 0.46250871658700277 1101 80-81 0.4480897553397938 1101 82-83 0.4615144960525832 1101 84-85 0.39948434930595056 1101 86-87 0.3625221705163959 1101 88-89 0.34808047024256794 1101 90-91 0.3411974352598328 1101 92-93 0.33487314270056956 1101 94-95 0.32216106744280637 1101 96-97 0.27760527442294247 1101 98-99 0.2663688256236263 1101 100 0.2709618344131215 1102 1 0.651082025722868 1102 2 0.6426895060630606 1102 3 0.6325925357266229 1102 4 0.5843766220259283 1102 5 0.5569407226089993 1102 6 0.6201491495038383 1102 7 0.5716103805262662 1102 8 0.6070693375830771 1102 9 0.7432401808303055 1102 10-11 0.6797448106578514 1102 12-13 0.7016816974012343 1102 14-15 0.8265816186548065 1102 16-17 0.8406896747883721 1102 18-19 0.8079242330209198 1102 20-21 0.7566044672492964 1102 22-23 0.725163306459315 1102 24-25 0.7639627690434025 1102 26-27 0.7807043112044241 1102 28-29 0.7700838579325406 1102 30-31 0.7887901543085079 1102 32-33 0.7587871070524201 1102 34-35 0.6984837260169101 1102 36-37 0.7427303248183037 1102 38-39 0.7928976411366477 1102 40-41 0.7121214734387848 1102 42-43 0.7009460378129404 1102 44-45 0.6513650478162276 1102 46-47 0.7484010065995932 1102 48-49 0.7547423383760261 1102 50-51 0.7070489280875591 1102 52-53 0.7457376399840498 1102 54-55 0.7884071746357648 1102 56-57 0.7819506398535765 1102 58-59 0.7839421364319392 1102 60-61 0.7571739746733925 1102 62-63 0.7033710027917479 1102 64-65 0.6687873726850526 1102 66-67 0.6472052684780891 1102 68-69 0.643823063257642 1102 70-71 0.6836877017372203 1102 72-73 0.6646140093233086 1102 74-75 0.5936298901573025 1102 76-77 0.5776398637964419 1102 78-79 0.5300953422412178 1102 80-81 0.5037003902025461 1102 82-83 0.5382842921352129 1102 84-85 0.4701949469102189 1102 86-87 0.4016293758516234 1102 88-89 0.42214770695708737 1102 90-91 0.3467598394283904 1102 92-93 0.3333666677131788 1102 94-95 0.3185402769916337 1102 96-97 0.2511862681084587 1102 98-99 0.26052792950688186 1102 100 0.2737039772118166 1103 1 0.5534262727508654 1103 2 0.5463875103893479 1103 3 0.5325623827654766 1103 4 0.486275671232022 1103 5 0.4688242832839151 1103 6 0.4940824042992915 1103 7 0.4932340818345722 1103 8 0.4929358443583638 1103 9 0.5892095712831775 1103 10-11 0.5650451853317406 1103 12-13 0.5666696060168874 1103 14-15 0.731515406194454 1103 16-17 0.7031651351084705 1103 18-19 0.6656769659329385 1103 20-21 0.6136940272659857 1103 22-23 0.6484188273205689 1103 24-25 0.6552796714273939 1103 26-27 0.6957477550682896 1103 28-29 0.6542528259593681 1103 30-31 0.6436414461984228 1103 32-33 0.6294016522238621 1103 34-35 0.5890476770657145 1103 36-37 0.6216193040797933 1103 38-39 0.6456008384359109 1103 40-41 0.6102148015992412 1103 42-43 0.5790746187041407 1103 44-45 0.539603976071696 1103 46-47 0.6170958659746049 1103 48-49 0.6179909385430804 1103 50-51 0.5401318067082101 1103 52-53 0.6024682625966093 1103 54-55 0.6690719050581606 1103 56-57 0.6582851596716495 1103 58-59 0.7474414471403037 1103 60-61 0.6885275759010838 1103 62-63 0.6369943262002877 1103 64-65 0.6193834435478891 1103 66-67 0.576201042567611 1103 68-69 0.550151324205622 1103 70-71 0.5686889514373661 1103 72-73 0.5412804471066153 1103 74-75 0.5225197860118911 1103 76-77 0.5516645629453834 1103 78-79 0.4778274123745305 1103 80-81 0.4119366975012895 1103 82-83 0.3875508973091506 1103 84-85 0.3825197900180157 1103 86-87 0.3932976762641225 1103 88-89 0.3818117219221797 1103 90-91 0.3634176565438274 1103 92-93 0.3654461864562393 1103 94-95 0.3768038232272133 1103 96-97 0.31826999598932915 1103 98-99 0.2560956909641554 1103 100 0.32863212743898984 1104 1 0.5645720538743326 1104 2 0.5672406041044376 1104 3 0.5462971490124744 1104 4 0.48103935717860224 1104 5 0.46963233506971847 1104 6 0.4942471134617321 1104 7 0.46953058386656465 1104 8 0.506858184807669 1104 9 0.6155414492138078 1104 10-11 0.6130534793181255 1104 12-13 0.60287407324833 1104 14-15 0.7410514686472993 1104 16-17 0.7052014290139752 1104 18-19 0.7206296201185367 1104 20-21 0.6803254812037167 1104 22-23 0.6211080341065838 1104 24-25 0.65363536078209 1104 26-27 0.6849170652613239 1104 28-29 0.6531828158747714 1104 30-31 0.6686593718079479 1104 32-33 0.6690343856248049 1104 34-35 0.6662914678381853 1104 36-37 0.6269315949077736 1104 38-39 0.6521540950724045 1104 40-41 0.6485719914056745 1104 42-43 0.6274977680849361 1104 44-45 0.6219087763466646 1104 46-47 0.6441629297821123 1104 48-49 0.6714879488132013 1104 50-51 0.6438601951485694 1104 52-53 0.6514351182208671 1104 54-55 0.7465938611364322 1104 56-57 0.7555710801491244 1104 58-59 0.7687660516045582 1104 60-61 0.7592240728251554 1104 62-63 0.7188068577035693 1104 64-65 0.6430177930382115 1104 66-67 0.658504594239723 1104 68-69 0.6727905691414193 1104 70-71 0.6543422208081822 1104 72-73 0.6445489417704309 1104 74-75 0.6097494129456678 1104 76-77 0.639978385085449 1104 78-79 0.5832153536376907 1104 80-81 0.5641905001532237 1104 82-83 0.5875479957263714 1104 84-85 0.5436903729346483 1104 86-87 0.5351645787464712 1104 88-89 0.5910264074782212 1104 90-91 0.5123420915953751 1104 92-93 0.5032218962355302 1104 94-95 0.50988502391494 1104 96-97 0.5024498253067371 1104 98-99 0.49589792220327666 1104 100 0.46406558949902177 1105 1 0.44880090970337605 1105 2 0.44048328567761175 1105 3 0.41666970993846775 1105 4 0.41527304209436977 1105 5 0.40907187513346344 1105 6 0.4253063541273505 1105 7 0.38612463982141776 1105 8 0.43061823040631 1105 9 0.4952395726103802 1105 10-11 0.48444690524272005 1105 12-13 0.5018393596223518 1105 14-15 0.611769047329453 1105 16-17 0.5777613832894346 1105 18-19 0.574832598861839 1105 20-21 0.534115971358311 1105 22-23 0.5079365443526669 1105 24-25 0.5047231287939553 1105 26-27 0.5009834236663693 1105 28-29 0.5097640952398308 1105 30-31 0.553300226432377 1105 32-33 0.5134709033293845 1105 34-35 0.5288129087537428 1105 36-37 0.5231002211083293 1105 38-39 0.5519959370729453 1105 40-41 0.5036104591957979 1105 42-43 0.4840537716232518 1105 44-45 0.44925590690203165 1105 46-47 0.5204698344057164 1105 48-49 0.537427469108188 1105 50-51 0.48526379526713725 1105 52-53 0.5227734584614652 1105 54-55 0.6169590509024943 1105 56-57 0.5809667718431797 1105 58-59 0.6277757944263485 1105 60-61 0.6248639438632964 1105 62-63 0.5920652323333044 1105 64-65 0.5130603289978595 1105 66-67 0.4604600143928863 1105 68-69 0.4864207991849554 1105 70-71 0.5271152791071572 1105 72-73 0.495575197773924 1105 74-75 0.5066986765869856 1105 76-77 0.46747220841142934 1105 78-79 0.43161776389290907 1105 80-81 0.43556666004378997 1105 82-83 0.4720646244717521 1105 84-85 0.4101429854967904 1105 86-87 0.4011808680788569 1105 88-89 0.4413484882198979 1105 90-91 0.35221974751930674 1105 92-93 0.38027290706206074 1105 94-95 0.43942778972337493 1105 96-97 0.40771313314524704 1105 98-99 0.41790809298771237 1105 100 0.410135451860544 1106 1 0.37376702316628396 1106 2 0.3538717755526015 1106 3 0.3181426922098538 1106 4 0.33498244641188535 1106 5 0.3167506321468494 1106 6 0.3423336661513048 1106 7 0.31162690335045085 1106 8 0.36792439256439025 1106 9 0.4462260958365647 1106 10-11 0.4143416555935886 1106 12-13 0.41170274973514154 1106 14-15 0.47765054656439077 1106 16-17 0.47716564361120817 1106 18-19 0.4272078441584952 1106 20-21 0.4266445824203018 1106 22-23 0.37003783631634946 1106 24-25 0.4231422357681538 1106 26-27 0.4348815525918681 1106 28-29 0.4606697167605134 1106 30-31 0.44306408710266254 1106 32-33 0.42366651537904687 1106 34-35 0.38231832184821 1106 36-37 0.4256644632233275 1106 38-39 0.44328759740565715 1106 40-41 0.39312528527743495 1106 42-43 0.3978234076713534 1106 44-45 0.33179137761916877 1106 46-47 0.3889799734701711 1106 48-49 0.3902712029878117 1106 50-51 0.3591533092930703 1106 52-53 0.3778996057383992 1106 54-55 0.35895545490115666 1106 56-57 0.397200797559762 1106 58-59 0.43962334165506434 1106 60-61 0.4253876599612738 1106 62-63 0.4268400042744176 1106 64-65 0.34607987264966766 1106 66-67 0.39473718863779794 1106 68-69 0.36485051462716456 1106 70-71 0.4558461493271224 1106 72-73 0.42720695227066585 1106 74-75 0.3953883013255748 1106 76-77 0.3956335876042729 1106 78-79 0.36211864911120273 1106 80-81 0.3466748463455289 1106 82-83 0.3857042749060575 1106 84-85 0.3440123752458959 1106 86-87 0.3372769618235907 1106 88-89 0.2935416356075464 1106 90-91 0.3148663730247421 1106 92-93 0.329347445301412 1106 94-95 0.348383630768744 1106 96-97 0.2816057580852025 1106 98-99 0.27694336209107107 1106 100 0.327930255439977 1107 1 0.48798085923675316 1107 2 0.47445796223259507 1107 3 0.404092256267397 1107 4 0.4239713205257729 1107 5 0.4467045816672268 1107 6 0.47067609366841623 1107 7 0.4388521249694364 1107 8 0.4509515666430701 1107 9 0.5607813831703012 1107 10-11 0.5155781634078238 1107 12-13 0.49342863478916854 1107 14-15 0.6276599635262912 1107 16-17 0.6628705010500724 1107 18-19 0.6310198622561458 1107 20-21 0.5740395733357175 1107 22-23 0.5390353270463706 1107 24-25 0.5747304397514483 1107 26-27 0.5657536930956368 1107 28-29 0.5809803241137601 1107 30-31 0.5863563676177321 1107 32-33 0.5652255697375281 1107 34-35 0.5516443467167633 1107 36-37 0.5426769524570574 1107 38-39 0.5924212621085303 1107 40-41 0.5391600351627872 1107 42-43 0.5460358647952575 1107 44-45 0.5014356509232059 1107 46-47 0.5587710760250673 1107 48-49 0.5355907701339362 1107 50-51 0.44681564209339086 1107 52-53 0.48248862398651937 1107 54-55 0.516807877620991 1107 56-57 0.49308717989849526 1107 58-59 0.5305326496403566 1107 60-61 0.479965845343429 1107 62-63 0.4485590629910021 1107 64-65 0.46364665352459156 1107 66-67 0.3833565225917255 1107 68-69 0.4517469633237994 1107 70-71 0.46210749665822703 1107 72-73 0.4367262396437539 1107 74-75 0.48117563037616407 1107 76-77 0.44792988520714516 1107 78-79 0.43789263506592135 1107 80-81 0.4662570238571888 1107 82-83 0.4341947747897841 1107 84-85 0.43846109540061917 1107 86-87 0.4260355439420671 1107 88-89 0.43639329757893464 1107 90-91 0.4331377053592611 1107 92-93 0.42826037776110937 1107 94-95 0.43269979950515136 1107 96-97 0.44750761337548894 1107 98-99 0.4796045153713244 1107 100 0.4417688673356359 1108 1 0.21664785981012713 1108 2 0.15789297946767178 1108 3 0.19870087151092264 1108 4 0.24661724443918587 1108 5 0.24286360203538493 1108 6 0.2534372063885044 1108 7 0.23108226746683158 1108 8 0.24301097531290594 1108 9 0.2767889924598137 1108 10-11 0.2594193479779108 1108 12-13 0.22489138264622 1108 14-15 0.3201139413776488 1108 16-17 0.2894081662224153 1108 18-19 0.28177821709297746 1108 20-21 0.225204933277098 1108 22-23 0.18123405994247577 1108 24-25 0.2134176188598218 1108 26-27 0.2216401771255434 1108 28-29 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78-79 -1.6738845557352882 2316 80-81 -1.636616367819748 2316 82-83 -1.8920887056972369 2316 84-85 -1.5942689916867252 2316 86-87 -1.4785987906599445 2316 88-89 -1.65847459702211 2316 90-91 -1.5349121122129645 2316 92-93 -1.4558974489168506 2316 94-95 -1.4739257464339204 2316 96-97 -1.3627571919294148 2316 98-99 -1.4101226706540082 2316 100 -1.5418998366404395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 19.0 4 82.0 5 184.0 6 1405.0 7 16630.0 8 23915.0 9 30757.0 10 34091.0 11 35445.0 12 39880.0 13 47489.0 14 59115.0 15 75375.0 16 92198.0 17 109531.0 18 122255.0 19 135071.0 20 152787.0 21 181612.0 22 228286.0 23 305824.0 24 407761.0 25 475767.0 26 477924.0 27 443911.0 28 433827.0 29 466890.0 30 538827.0 31 647751.0 32 798334.0 33 1018145.0 34 1376027.0 35 2017654.0 36 3310147.0 37 6033835.0 38 7540081.0 39 2339539.0 40 5685.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.64487758415367 33.88368020416714 27.063865744136223 21.40757646754297 2 21.487910709247917 30.995203351634437 28.131238548486298 19.38564739063135 3 23.856214636423665 26.849797831060627 27.036654878141718 22.257332654373986 4 20.00926458154074 27.43757648458957 26.995427194930777 25.557731738938905 5 21.203146746215733 31.28913756779515 26.64996708289282 20.857748603096294 6 25.400438901392935 26.721153197955665 26.92711138028786 20.951296520363538 7 21.040638213571146 27.21556674421337 30.979315386302236 20.764479655913245 8 24.7615045748649 26.688669245754138 27.27983187881078 21.269994300570183 9 24.916223843973643 26.457178204037458 27.414224114156994 21.212373837831905 10-11 20.69255503116676 29.192721122991568 29.161765943370504 20.952957902471166 12-13 20.795011131911107 27.020211633708918 28.955125800004627 23.22965143437535 14-15 20.79368124013624 31.511533618242655 26.65757751051357 21.037207631107535 16-17 23.141353719830526 26.962747804627064 26.61944975056002 23.276448724982394 18-19 20.92237138113518 29.199129524671818 26.539876890717228 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21.29266102586369 27.221123877581764 30.44240861399369 21.04380648256086 46-47 21.320874257308716 27.297614907278533 30.363876117146344 21.017634718266407 48-49 21.39630246409551 27.286565396102496 30.26395083403221 21.053181305769787 50-51 21.437171586876026 27.323365564359797 30.20694192346734 21.032520925296836 52-53 21.544174752219455 27.465817494138793 30.02305336633087 20.966954387310878 54-55 21.866887789280163 27.626041205686843 29.423591410294 21.083479594738993 56-57 22.193657301611662 27.973313635348774 28.681575186429793 21.15145387660977 58-59 22.394314029454378 28.333675714018664 28.1663392712101 21.105670985316856 60-61 22.536137510756078 28.52067115487153 27.865347988841688 21.077843345530706 62-63 22.630657830708152 28.617849894205534 27.7540757657826 20.99741650930372 64-65 22.783677278343387 28.656300147347846 27.69374447845691 20.866278095851854 66-67 22.93583197748104 28.498083916183692 27.750627210170364 20.815456896164903 68-69 23.052542917111772 28.345220848146173 27.884152994429236 20.718083240312815 70-71 23.17561663354639 28.267689994468352 27.96745405195647 20.589239320028785 72-73 23.34968734959118 28.233367195026037 27.919294705077952 20.497650750304828 74-75 23.525602102206875 28.243091562081823 27.838016267191247 20.39329006852006 76-77 23.795234211440526 28.314802303000715 27.586186327786884 20.303777157771876 78-79 24.05397452438862 28.380524355501645 27.30607470306038 20.259426417049355 80-81 24.273285732366958 28.461427388233968 27.04272486368539 20.22256201571368 82-83 24.44692215694403 28.567966809315067 26.828480019011348 20.156631014729562 84-85 24.611775534665508 28.582436808478985 26.67644062159101 20.129347035264498 86-87 24.82026094539533 28.5212964855504 26.57056845763146 20.08787411142281 88-89 24.975749281143273 28.529298171015732 26.472740232418317 20.022212315422678 90-91 25.16883087712675 28.470838112178765 26.333281212592368 20.027049798102112 92-93 25.31882205897383 28.422363779104217 26.30294941124842 19.95586475067353 94-95 25.49391527571937 28.44299163459653 26.21991030975298 19.843182779931116 96-97 25.593365427111202 28.427617275933688 26.127677240986397 19.851340055968716 98-99 25.67405925365035 28.431554274708688 26.076438348938275 19.81794812270269 100 25.710765106004736 28.498924625406712 25.988478707763 19.80183156082555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 55461.0 1 34742.0 2 11735.5 3 9123.0 4 8764.0 5 8980.5 6 9465.0 7 9896.5 8 10195.5 9 10513.0 10 11223.0 11 13214.5 12 80541.5 13 99417.0 14 52108.0 15 55428.5 16 63021.0 17 70587.5 18 77602.5 19 85279.0 20 94989.5 21 110513.0 22 133316.5 23 159786.0 24 187882.5 25 219415.0 26 255166.0 27 298465.5 28 353767.0 29 408989.0 30 456320.5 31 501387.5 32 549005.0 33 593392.0 34 631614.5 35 678505.0 36 732212.5 37 781944.0 38 835705.0 39 875556.0 40 894485.5 41 925553.5 42 970544.0 43 993081.5 44 1004801.5 45 1031083.5 46 1058024.0 47 1075343.0 48 1077249.0 49 1069156.0 50 1058464.0 51 1045353.5 52 1022772.5 53 973841.0 54 906264.5 55 839489.5 56 770009.0 57 694287.5 58 612793.0 59 523345.5 60 428587.0 61 343806.5 62 270003.5 63 202827.0 64 149847.5 65 110497.0 66 80832.0 67 59995.0 68 45325.5 69 35287.0 70 27904.0 71 22188.5 72 18613.0 73 15482.0 74 12662.0 75 10754.0 76 8689.5 77 6185.0 78 4353.5 79 3324.0 80 2551.5 81 2005.0 82 1580.0 83 1261.0 84 1011.0 85 848.5 86 707.0 87 568.0 88 480.0 89 435.0 90 374.5 91 299.0 92 261.5 93 236.0 94 205.5 95 174.0 96 139.5 97 111.5 98 91.5 99 64.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047075584990915284 2 0.015164506754184045 3 0.005462286641085402 4 1.1324252792494125E-4 5 5.129220382482634E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.995993879041526E-6 12-13 3.663728844630452E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.038457495549568656 22-23 0.0758075457892831 24-25 0.03144811613727339 26-27 0.03565640831471937 28-29 0.04036096921748347 30-31 0.0338794998250736 32-33 0.034683854839599286 34-35 0.035489875185417984 36-37 0.03322169396433313 38-39 0.04322200837888126 40-41 0.06424515062188799 42-43 0.07241859660799993 44-45 0.07525465580000251 46-47 0.04811808238034195 48-49 0.030468901336981255 50-51 0.02801919900495789 52-53 0.028254010717272845 54-55 0.029086676363779762 56-57 0.029318157413508687 58-59 0.03719517442946416 60-61 0.05722577922183465 62-63 0.08040719082058734 64-65 0.10804669429073806 66-67 0.08970806609206963 68-69 0.05292922448585893 70-71 0.04660096557240634 72-73 0.050691019228048334 74-75 0.048128074368100036 76-77 0.0512289212356918 78-79 0.04382152764436624 80-81 0.042267773547984326 82-83 0.041643274313104134 84-85 0.048992381309174216 86-87 0.05295920044913319 88-89 0.059049316987684806 90-91 0.05598177674595331 92-93 0.051255566536380026 94-95 0.053288936045149934 96-97 0.055215724351166943 98-99 0.049685159127067974 100 0.05143542231602552 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0024056E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.87722353444839 #Duplication Level Percentage of deduplicated Percentage of total 1 68.83492716033875 28.137807045106396 2 15.676573385846673 12.816295890948776 3 5.714044602737189 7.007228355356893 4 2.7439126901495854 4.486541295770169 5 1.567185507926879 3.203109616373754 6 1.0509721026205705 2.577649294037417 7 0.7800025699975729 2.2319037587864505 8 0.5715744548883253 1.8691501407240445 9 0.4191934585439915 1.5421918238173138 >10 2.3375553361001375 17.379878025198945 >50 0.17390770308891085 4.948390841380658 >100 0.1292734186300399 9.173166349160438 >500 4.292556151941079E-4 0.11024033621042842 >1k 4.1591886482921154E-4 0.3177885006223494 >5k 0.0 0.0 >10k+ 3.243465146198079E-5 4.198658726506074 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1138015 3.7903439828382948 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89779 0.29902355631097943 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0028810231369139467 3.3306625860276837E-6 0.0 0.0 2.331463810219379E-5 2 0.0029975963274249157 6.6613251720553675E-6 0.0 0.0 2.664530068822147E-5 3 0.003087524217247663 1.3322650344110735E-5 0.0 6.6613251720553675E-6 2.664530068822147E-5 4 0.003180782769656438 1.665331293013842E-5 0.0 6.6613251720553675E-6 2.664530068822147E-5 5 0.0032507266839630197 3.663728844630452E-5 0.0 6.6613251720553675E-6 2.9975963274249154E-5 6 0.0033473158989578223 3.663728844630452E-5 3.3306625860276837E-6 9.991987758083052E-6 2.9975963274249154E-5 7 0.0033872838499901547 4.329861361835989E-5 3.3306625860276837E-6 9.991987758083052E-6 2.9975963274249154E-5 8 0.0034738810772268745 5.329060137644294E-5 3.3306625860276837E-6 1.3322650344110735E-5 2.9975963274249154E-5 9 0.003550486316705511 6.661325172055368E-5 3.3306625860276837E-6 1.665331293013842E-5 3.330662586027684E-5 10-11 0.003685378151439632 9.159322111576131E-5 3.3306625860276837E-6 2.497996939520763E-5 3.830261973931836E-5 12-13 0.003823600648759781 1.0158520887384437E-4 3.3306625860276837E-6 2.8310631981235314E-5 5.16252700834291E-5 14-15 0.004031767060386511 1.1657319051096895E-4 3.3306625860276837E-6 2.9975963274249154E-5 5.495593266945678E-5 16-17 0.004231606815548172 1.2989584085507967E-4 3.3306625860276837E-6 3.497195715329068E-5 5.828659525548447E-5 18-19 0.004504721147602442 1.382224973201489E-4 3.3306625860276837E-6 4.163328232534605E-5 6.661325172055367E-5 20-21 0.00473786752862438 1.58206472836315E-4 3.3306625860276837E-6 4.496394491137374E-5 6.994391430658136E-5 22-23 0.004977675234818374 1.7319445447343956E-4 3.3306625860276837E-6 4.995993879041526E-5 7.827057077165057E-5 24-25 0.005818667537790364 1.865171048175503E-4 3.3306625860276837E-6 5.662126396247063E-5 8.160123335767825E-5 26-27 0.00827003520110674 1.9650909257563334E-4 3.3306625860276837E-6 5.662126396247063E-5 8.659722723671978E-5 28-29 0.014798133869721002 2.1482773679878562E-4 3.3306625860276837E-6 5.662126396247063E-5 8.826255852973362E-5 30-31 0.030019261887867514 2.298157184359102E-4 3.3306625860276837E-6 5.995192654849831E-5 8.992788982274746E-5 32-33 0.06136246215368103 2.3647704360796557E-4 3.3306625860276837E-6 6.827858301356752E-5 9.325855240877516E-5 34-35 0.11568057293791352 2.6811833817522857E-4 3.3306625860276837E-6 7.493990818562289E-5 9.325855240877516E-5 36-37 0.1976115418916085 2.697836694682424E-4 3.3306625860276837E-6 7.827057077165057E-5 9.991987758083052E-5 38-39 0.3121163909366543 2.781103259333116E-4 3.3306625860276837E-6 7.993590206466442E-5 1.0158520887384437E-4 40-41 0.4630453660224988 2.9809430144947773E-4 3.3306625860276837E-6 7.993590206466442E-5 1.065812027528859E-4 42-43 0.6517840227849295 3.3473158989578224E-4 3.3306625860276837E-6 7.993590206466442E-5 1.1324252792494125E-4 44-45 0.8806521677151149 3.563808967049622E-4 3.3306625860276837E-6 7.993590206466442E-5 1.1823852180398278E-4 46-47 1.1515299598428674 3.746995409281144E-4 3.3306625860276837E-6 8.32665646506921E-5 1.2823050956206584E-4 48-49 1.464773780064892 3.846915286861975E-4 3.3306625860276837E-6 8.493189594370595E-5 1.4488382249220426E-4 50-51 1.8194327242128778 3.9967951032332207E-4 3.3306625860276837E-6 8.992788982274746E-5 1.4821448507823195E-4 52-53 2.2127223583649056 4.013448416163359E-4 3.3306625860276837E-6 9.4923883701789E-5 1.5321047895727345E-4 54-55 2.64550698946205 4.1633282325346046E-4 3.3306625860276837E-6 9.658921499480284E-5 1.5321047895727345E-4 56-57 3.1170405490850404 4.279901423045574E-4 3.3306625860276837E-6 9.991987758083052E-5 1.5321047895727345E-4 58-59 3.6250215493869318 4.3465146747661274E-4 3.3306625860276837E-6 9.991987758083052E-5 1.5321047895727345E-4 60-61 4.166533995273657 4.4464345523469585E-4 3.3306625860276837E-6 9.991987758083052E-5 1.5321047895727345E-4 62-63 4.739874252832462 4.4963944911373735E-4 3.3306625860276837E-6 1.032505401668582E-4 1.58206472836315E-4 64-65 5.344820833001378 4.7461941850894496E-4 3.3306625860276837E-6 1.032505401668582E-4 1.6819846059439804E-4 66-67 5.978750838993905 4.929380627320972E-4 3.3306625860276837E-6 1.0824653404589973E-4 1.748597857664534E-4 68-69 6.640456905622612 5.06260713076208E-4 3.3306625860276837E-6 1.1157719663192742E-4 1.8152111093850879E-4 70-71 7.326933109903605 5.195833634203187E-4 3.3306625860276837E-6 1.1324252792494125E-4 1.8318644223152262E-4 72-73 8.036723952286794 5.345713450574433E-4 3.3306625860276837E-6 1.232345156830243E-4 1.8818243611056415E-4 74-75 8.771394844187608 5.47893995401554E-4 3.3306625860276837E-6 1.232345156830243E-4 1.9317842998960567E-4 76-77 9.527809966781303 5.662126396247062E-4 3.3306625860276837E-6 1.2489984697603814E-4 2.015050864546749E-4 78-79 10.302263957940927 5.861966151408724E-4 3.3306625860276837E-6 1.2656517826905198E-4 2.0483574904070256E-4 80-81 11.099389769323638 6.01184596777997E-4 3.3306625860276837E-6 1.2989584085507967E-4 2.065010803337164E-4 82-83 11.90937859961359 6.211685722941631E-4 3.3306625860276837E-6 1.3988782861316273E-4 2.1149707421275793E-4 84-85 12.732688414916359 6.378218852243014E-4 3.3306625860276837E-6 1.3988782861316273E-4 2.1649306809179945E-4 86-87 13.573827267042134 6.52809866861426E-4 3.3306625860276837E-6 1.3988782861316273E-4 2.2148906197084098E-4 88 14.213462698044529 6.628018546195091E-4 3.3306625860276837E-6 1.3988782861316273E-4 2.2315439326385482E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 143350 0.0 85.85509 8 AGTGGTA 146420 0.0 84.24814 5 TGGTATC 146450 0.0 83.83567 7 GTATCAA 147115 0.0 83.59082 9 GTGGTAT 147615 0.0 83.47951 6 GCAGTGG 157185 0.0 79.050415 3 AAGCAGT 158585 0.0 78.20626 1 CAGTGGT 159880 0.0 77.16719 4 AGCAGTG 166070 0.0 74.86548 2 CAACGCA 139975 0.0 44.97432 12-13 ATCAACG 141625 0.0 44.56146 10-11 TCAACGC 139980 0.0 44.232815 12-13 ACGCAGA 143050 0.0 44.029823 14-15 AACGCAG 141700 0.0 43.718098 14-15 AGAGTAC 143830 0.0 43.16752 18-19 GAGTACT 141110 0.0 42.919815 20-21 CGCAGAG 144485 0.0 42.808907 16-17 TATCAAC 146485 0.0 41.9944 10-11 AGTACTT 152350 0.0 40.514194 20-21 GTACTTT 152035 0.0 39.87455 22-23 >>END_MODULE