FastQCFastQC Report
Thu 26 May 2016
SRR1660755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30024056
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12292354.09416702393574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA724520.24131316568287772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1545100.085.8681268
AGTGGTA1579350.084.4373555
GTATCAA1577150.083.971219
TGGTATC1583250.083.911847
GTGGTAT1592300.083.706426
GCAGTGG1675250.079.6317753
AAGCAGT1660650.078.634631
CAGTGGT1706500.077.9890064
AGCAGTG1763200.075.6116942
CAACGCA1503700.044.98804512-13
ATCAACG1521350.044.51244410-11
TCAACGC1503250.044.07306712-13
ACGCAGA1538850.044.07016814-15
AACGCAG1519900.043.6346814-15
AGAGTAC1565400.043.13430418-19
GAGTACT1527800.042.92579320-21
CGCAGAG1554550.042.8102416-17
TATCAAC1578600.041.98573710-11
AGTACTT1640300.040.91300220-21
GTACTTT1639100.040.0314722-23