##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660755_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30024056 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.973074090988906 33.0 31.0 34.0 26.0 34.0 2 31.771122662441076 34.0 31.0 34.0 28.0 34.0 3 32.28416080758709 34.0 31.0 34.0 30.0 34.0 4 35.81602372444283 37.0 35.0 37.0 35.0 37.0 5 35.877763983653644 37.0 35.0 37.0 35.0 37.0 6 35.912584395659266 37.0 35.0 37.0 35.0 37.0 7 35.92653720736465 37.0 35.0 37.0 35.0 37.0 8 35.91910996302432 37.0 35.0 37.0 35.0 37.0 9 37.630322765185355 39.0 37.0 39.0 35.0 39.0 10-11 37.59044850569157 39.0 37.0 39.0 35.0 39.0 12-13 37.627028273595016 39.0 37.0 39.0 35.0 39.0 14-15 38.94953451658896 40.0 38.0 41.0 35.0 41.0 16-17 38.965482395183386 40.0 38.0 41.0 35.0 41.0 18-19 38.95538973814864 40.0 38.0 41.0 35.0 41.0 20-21 38.90534744872578 40.0 38.0 41.0 35.0 41.0 22-23 38.82414859271512 40.0 38.0 41.0 34.5 41.0 24-25 38.754143610710024 40.0 38.0 41.0 34.0 41.0 26-27 38.691720066069685 40.0 38.0 41.0 34.0 41.0 28-29 38.58020128925952 40.0 38.0 41.0 34.0 41.0 30-31 38.48359159068981 40.0 38.0 41.0 34.0 41.0 32-33 38.38006140809223 40.0 38.0 41.0 34.0 41.0 34-35 38.27321100453583 40.0 38.0 41.0 34.0 41.0 36-37 38.10341882189402 40.0 38.0 41.0 33.0 41.0 38-39 37.75637643694776 40.0 37.0 41.0 33.0 41.0 40-41 37.614478287010925 40.0 36.0 41.0 33.0 41.0 42-43 37.46399452159295 40.0 36.0 41.0 32.5 41.0 44-45 37.350839257027765 40.0 36.0 41.0 32.0 41.0 46-47 37.22275199926352 39.0 35.0 41.0 32.0 41.0 48-49 37.354886445055925 40.0 36.0 41.0 32.0 41.0 50-51 37.27713848855065 40.0 36.0 41.0 32.0 41.0 52-53 37.05908112481538 39.0 35.0 41.0 31.0 41.0 54-55 36.65973801141325 39.0 35.0 41.0 30.0 41.0 56-57 36.456834229858885 39.0 35.0 41.0 29.5 41.0 58-59 36.28586865478802 39.0 35.0 41.0 29.0 41.0 60-61 36.08882582353297 39.0 35.0 41.0 29.0 41.0 62-63 35.800750005262444 38.5 35.0 40.5 28.0 41.0 64-65 35.550047701749556 38.0 35.0 40.0 28.0 41.0 66-67 35.278550706140436 37.0 34.5 40.0 28.0 41.0 68-69 34.98022187608496 37.0 34.0 40.0 28.0 41.0 70-71 34.64317947248699 36.0 34.0 39.0 27.5 41.0 72-73 34.32790026770534 36.0 34.0 39.0 27.0 41.0 74-75 33.97767451872592 35.0 34.0 38.5 26.0 40.0 76-77 31.89025415153769 34.0 31.0 35.5 23.5 38.5 78-79 33.16142112844447 35.0 33.0 37.0 26.0 39.0 80-81 33.149236365666255 35.0 33.0 36.0 26.0 39.0 82-83 32.93819655811993 35.0 33.0 36.0 26.0 37.5 84-85 32.71166891974889 35.0 33.0 36.0 26.0 37.0 86-87 32.51349479563987 35.0 33.0 35.0 26.0 37.0 88-89 32.299293423247015 35.0 33.0 35.0 25.0 36.0 90-91 32.146349763669505 35.0 33.0 35.0 25.0 36.0 92-93 32.02547239120524 35.0 33.0 35.0 25.0 36.0 94-95 31.932016330505114 35.0 33.0 35.0 24.5 35.5 96-97 31.829461232686214 35.0 33.0 35.0 24.0 35.0 98-99 31.714129113668054 35.0 33.0 35.0 24.0 35.0 100 31.528816359788298 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7531239531741072 1101 2 1.2161611325212114 1101 3 0.7832184080355802 1101 4 0.6070968285472276 1101 5 0.4549232954570641 1101 6 0.40689219681643607 1101 7 0.38270130099176214 1101 8 0.4170678821582712 1101 9 0.48835213932834876 1101 10-11 0.49184646722430614 1101 12-13 0.47004722195247695 1101 14-15 0.6378590441101863 1101 16-17 0.6279929648581799 1101 18-19 0.5500738147038717 1101 20-21 0.5868795935334816 1101 22-23 0.5513342520561366 1101 24-25 0.6052375428039838 1101 26-27 0.5904023381053634 1101 28-29 0.6139300510677828 1101 30-31 0.5826130372131715 1101 32-33 0.5684477272384498 1101 34-35 0.5847728155751781 1101 36-37 0.5859324914839732 1101 38-39 0.6562178226202064 1101 40-41 0.6693947858340081 1101 42-43 0.6363300831244416 1101 44-45 0.5988854149238207 1101 46-47 0.5430404018069765 1101 48-49 0.5379054210647567 1101 50-51 0.5289122391416967 1101 52-53 0.5189547105646781 1101 54-55 0.557602354368754 1101 56-57 0.6246633504157941 1101 58-59 0.620212100314312 1101 60-61 0.6070776109925475 1101 62-63 0.6564751937594266 1101 64-65 0.6183225691234853 1101 66-67 0.5622915527318213 1101 68-69 0.5482365811053285 1101 70-71 0.5671571476208754 1101 72-73 0.5179174619905993 1101 74-75 0.48797143626787687 1101 76-77 0.3885307020513906 1101 78-79 0.5167705006911447 1101 80-81 0.49664219905992013 1101 82-83 0.4441438022990809 1101 84-85 0.45617615845589654 1101 86-87 0.43349725138195794 1101 88-89 0.4512691921451406 1101 90-91 0.4625954509104204 1101 92-93 0.40360523356867617 1101 94-95 0.3309733828581827 1101 96-97 0.3312594645067719 1101 98-99 0.3496327577295979 1101 100 0.270350197457784 1102 1 1.6609633535455153 1102 2 1.138024314664161 1102 3 0.7063006901695061 1102 4 0.5613522718301667 1102 5 0.44066251305572024 1102 6 0.4035328047353559 1102 7 0.3615402641391512 1102 8 0.36902489315924214 1102 9 0.4508503159488413 1102 10-11 0.4336016698843821 1102 12-13 0.39542023546872684 1102 14-15 0.5714385427083641 1102 16-17 0.5651273729126203 1102 18-19 0.5359121747329212 1102 20-21 0.5378732034414568 1102 22-23 0.5382356087924123 1102 24-25 0.5692071960404874 1102 26-27 0.5575901900485718 1102 28-29 0.5726872096628242 1102 30-31 0.5662887410944322 1102 32-33 0.5551216308477436 1102 34-35 0.5363648603790878 1102 36-37 0.5949199526195059 1102 38-39 0.6549107936763789 1102 40-41 0.6161675576410275 1102 42-43 0.6016427506815063 1102 44-45 0.5708315648662179 1102 46-47 0.5835560912054447 1102 48-49 0.5246691760294908 1102 50-51 0.5035565795020105 1102 52-53 0.44617306606976825 1102 54-55 0.49282865850538116 1102 56-57 0.5595416916192306 1102 58-59 0.5643102456499633 1102 60-61 0.48879655159183244 1102 62-63 0.5446847557821783 1102 64-65 0.5000113657404839 1102 66-67 0.4978426009844057 1102 68-69 0.4729447900663075 1102 70-71 0.45521669289293243 1102 72-73 0.38900532433026314 1102 74-75 0.37890859404957666 1102 76-77 0.3216064263410843 1102 78-79 0.4340600177343319 1102 80-81 0.4148696469744806 1102 82-83 0.4363499193247833 1102 84-85 0.3881976690958524 1102 86-87 0.3662490540601624 1102 88-89 0.37234957743539354 1102 90-91 0.38146542157301866 1102 92-93 0.3348249087966977 1102 94-95 0.3003109905311163 1102 96-97 0.29477776438604053 1102 98-99 0.2852816807880565 1102 100 0.18720365972679076 1103 1 1.644932479299019 1103 2 1.090304260150564 1103 3 0.6749669860511744 1103 4 0.542761824595253 1103 5 0.40072438543638356 1103 6 0.3788161050433345 1103 7 0.32615449947033426 1103 8 0.3814866901113305 1103 9 0.43213552086310614 1103 10-11 0.4185830815125371 1103 12-13 0.40353836810684385 1103 14-15 0.5570543858694919 1103 16-17 0.5019818860633976 1103 18-19 0.43461710361354733 1103 20-21 0.443561521944531 1103 22-23 0.45862969402242726 1103 24-25 0.5223154328791182 1103 26-27 0.46223704686008915 1103 28-29 0.5138910539587371 1103 30-31 0.5251742998212094 1103 32-33 0.5167274547304359 1103 34-35 0.5193193766073776 1103 36-37 0.520020997736971 1103 38-39 0.5668310226166682 1103 40-41 0.5381364913565392 1103 42-43 0.4805901163936923 1103 44-45 0.5148715601450462 1103 46-47 0.5140745610711122 1103 48-49 0.4919923632902794 1103 50-51 0.4131898246410799 1103 52-53 0.424888299848611 1103 54-55 0.43715766444450566 1103 56-57 0.4585578504062795 1103 58-59 0.47468996466445645 1103 60-61 0.4702458160746943 1103 62-63 0.508549429885953 1103 64-65 0.4412188862483717 1103 66-67 0.41830370120463556 1103 68-69 0.43544231371330966 1103 70-71 0.4285934606290098 1103 72-73 0.40036976103566246 1103 74-75 0.36355349036609397 1103 76-77 0.23077636961994585 1103 78-79 0.3649564524475366 1103 80-81 0.3908495958835374 1103 82-83 0.38210307176548497 1103 84-85 0.3659505792713773 1103 86-87 0.3607274782987133 1103 88-89 0.41847212673527423 1103 90-91 0.38025411919139884 1103 92-93 0.36216448695224557 1103 94-95 0.33180822136787214 1103 96-97 0.32303924068040857 1103 98-99 0.32102664096030864 1103 100 0.26637810437847165 1104 1 1.5983944967645591 1104 2 1.0834563828995734 1104 3 0.6364932682545756 1104 4 0.5288134124669241 1104 5 0.41042527056101363 1104 6 0.3385338640424109 1104 7 0.3063539070224621 1104 8 0.3051951824907704 1104 9 0.4193028134274286 1104 10-11 0.3756173482119749 1104 12-13 0.35488334395240884 1104 14-15 0.5187815760767549 1104 16-17 0.48856813490214535 1104 18-19 0.43481889840921184 1104 20-21 0.39908684854108145 1104 22-23 0.42393912938080547 1104 24-25 0.46313215394159357 1104 26-27 0.4333587781898416 1104 28-29 0.44768246137181933 1104 30-31 0.45044490788725255 1104 32-33 0.44464760515869983 1104 34-35 0.4033395727586324 1104 36-37 0.4398204834686723 1104 38-39 0.5093018977134562 1104 40-41 0.5142461073366675 1104 42-43 0.4570746395710543 1104 44-45 0.4333104731369346 1104 46-47 0.4658344951789317 1104 48-49 0.45630951409206233 1104 50-51 0.4604634364483218 1104 52-53 0.4289866862067768 1104 54-55 0.44863717370100886 1104 56-57 0.5336581908988549 1104 58-59 0.5064769317336157 1104 60-61 0.5043322059133288 1104 62-63 0.5313726912153385 1104 64-65 0.5068446544317666 1104 66-67 0.4771416634138248 1104 68-69 0.46510444958329344 1104 70-71 0.4786806925651561 1104 72-73 0.44071216908688626 1104 74-75 0.40963641746009927 1104 76-77 0.3830773744260014 1104 78-79 0.4749029753546168 1104 80-81 0.4493699376460043 1104 82-83 0.44610959003995276 1104 84-85 0.48928439856661754 1104 86-87 0.49831004127600664 1104 88-89 0.48620626726197713 1104 90-91 0.4755551122918078 1104 92-93 0.4378325621990129 1104 94-95 0.42418883879489044 1104 96-97 0.3978685846921337 1104 98-99 0.41811167826408635 1104 100 0.325277744731185 1105 1 1.5736458633502046 1105 2 1.0429593403791593 1105 3 0.6127286814129462 1105 4 0.5050297099166343 1105 5 0.34666898201151497 1105 6 0.28306083811438043 1105 7 0.22056573941925706 1105 8 0.27046309779600364 1105 9 0.33744310819907497 1105 10-11 0.31287104036513824 1105 12-13 0.2937464084285537 1105 14-15 0.42445687943224897 1105 16-17 0.3659332114321856 1105 18-19 0.34291935272366203 1105 20-21 0.3267202741089861 1105 22-23 0.31547371347711106 1105 24-25 0.36217533909048427 1105 26-27 0.3420335423792764 1105 28-29 0.35082085106383687 1105 30-31 0.35562303561918185 1105 32-33 0.3734994594657124 1105 34-35 0.38729260479235705 1105 36-37 0.41236603768948044 1105 38-39 0.4481335373913282 1105 40-41 0.4457066139639281 1105 42-43 0.4337787108419562 1105 44-45 0.4257472919299943 1105 46-47 0.45522112182811014 1105 48-49 0.3959271802485489 1105 50-51 0.36907777640330863 1105 52-53 0.3283062033033488 1105 54-55 0.4037135031046546 1105 56-57 0.3743746896752569 1105 58-59 0.40829516254900966 1105 60-61 0.3547519443610838 1105 62-63 0.3660700025516448 1105 64-65 0.3287197845433596 1105 66-67 0.35790218851160205 1105 68-69 0.39418887244448797 1105 70-71 0.3667391269404803 1105 72-73 0.3074182854251859 1105 74-75 0.2343151140276305 1105 76-77 0.20599786379181495 1105 78-79 0.35448232157691706 1105 80-81 0.3046878846501073 1105 82-83 0.30419632751879533 1105 84-85 0.3429408328804513 1105 86-87 0.304829221798677 1105 88-89 0.3320286240123522 1105 90-91 0.3236983995891549 1105 92-93 0.28057399702853303 1105 94-95 0.2591693260717065 1105 96-97 0.2590380314768126 1105 98-99 0.2737526331420632 1105 100 0.2997295496261181 1106 1 1.4375223659333436 1106 2 0.8971494649343406 1106 3 0.5000417231044381 1106 4 0.42136573880853945 1106 5 0.24899613803120246 1106 6 0.2390114938652772 1106 7 0.19236265567889888 1106 8 0.20153192037847845 1106 9 0.27257145112827175 1106 10-11 0.24054611408501358 1106 12-13 0.201057435309842 1106 14-15 0.32649801794411104 1106 16-17 0.28114533382382234 1106 18-19 0.23820203050201627 1106 20-21 0.23363460921734713 1106 22-23 0.2239377968766405 1106 24-25 0.27483953677502626 1106 26-27 0.2559540039358694 1106 28-29 0.29048319690414104 1106 30-31 0.291485270797061 1106 32-33 0.2700654736032746 1106 34-35 0.25199239983012234 1106 36-37 0.2839435218424242 1106 38-39 0.37730255312102656 1106 40-41 0.3253009433108929 1106 42-43 0.29300776082557434 1106 44-45 0.2991336272727594 1106 46-47 0.33722199973019684 1106 48-49 0.34330789576637954 1106 50-51 0.34425990534968065 1106 52-53 0.27468943132739554 1106 54-55 0.3525629216827113 1106 56-57 0.3638014481847236 1106 58-59 0.2934244376759736 1106 60-61 0.31759832319937686 1106 62-63 0.3491873519771005 1106 64-65 0.35532788077211563 1106 66-67 0.31087633104627344 1106 68-69 0.3064679142137834 1106 70-71 0.33394540156007935 1106 72-73 0.3114408282349359 1106 74-75 0.25661952083363104 1106 76-77 0.21238974618493955 1106 78-79 0.3369013838285966 1106 80-81 0.2879309228963365 1106 82-83 0.2791137973377147 1106 84-85 0.3034653790578403 1106 86-87 0.27818304826922713 1106 88-89 0.3254751176150279 1106 90-91 0.3262825411483661 1106 92-93 0.2997492728358182 1106 94-95 0.3066101496873088 1106 96-97 0.3155450776109774 1106 98-99 0.34099919858352834 1106 100 0.30380116557975256 1107 1 1.5916629925644834 1107 2 1.0495154142176197 1107 3 0.6019472515858268 1107 4 0.5048350805259432 1107 5 0.33321481937335307 1107 6 0.32562531851251464 1107 7 0.27140551173496874 1107 8 0.29471577504048696 1107 9 0.36707391084159724 1107 10-11 0.32178284508411537 1107 12-13 0.3008059467507067 1107 14-15 0.4465915168086312 1107 16-17 0.4048711785605903 1107 18-19 0.35748214888119634 1107 20-21 0.34929198467358447 1107 22-23 0.3548348608915788 1107 24-25 0.40936066842314744 1107 26-27 0.37883877766360285 1107 28-29 0.36791894185807195 1107 30-31 0.4023457636900716 1107 32-33 0.41544545545570344 1107 34-35 0.40249508416822977 1107 36-37 0.45662566170062036 1107 38-39 0.48184450695823244 1107 40-41 0.46558171228598866 1107 42-43 0.42079965124707286 1107 44-45 0.42650588692844593 1107 46-47 0.4953138434268709 1107 48-49 0.45334022037606303 1107 50-51 0.4165121731360202 1107 52-53 0.4068248138338788 1107 54-55 0.3873799460811398 1107 56-57 0.3835449499650423 1107 58-59 0.34684102616793666 1107 60-61 0.28953184938364274 1107 62-63 0.32340296992683193 1107 64-65 0.3013626486562444 1107 66-67 0.3375201308624014 1107 68-69 0.30614637800108824 1107 70-71 0.32782906680292 1107 72-73 0.3294040274485539 1107 74-75 0.34520429952902276 1107 76-77 0.27207978198811134 1107 78-79 0.3910088423072082 1107 80-81 0.34408201689821283 1107 82-83 0.33540712130208306 1107 84-85 0.35876233253627277 1107 86-87 0.311989894717307 1107 88-89 0.3479708261058221 1107 90-91 0.3476528158985488 1107 92-93 0.4010592216736839 1107 94-95 0.37180640478906213 1107 96-97 0.33752192205999876 1107 98-99 0.3847422753526466 1107 100 0.31575688905993005 1108 1 1.3761899207743902 1108 2 0.8794860742674082 1108 3 0.46331591477139256 1108 4 0.3788880023372698 1108 5 0.217277064748842 1108 6 0.17521273503395918 1108 7 0.12399581856130482 1108 8 0.12664061360094792 1108 9 0.15117060385897219 1108 10-11 0.12048935369760727 1108 12-13 0.12485272942293335 1108 14-15 0.16433695513099877 1108 16-17 0.12676821533371196 1108 18-19 0.08451162080262264 1108 20-21 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9 403.0 10 206.0 11 218.0 12 236.0 13 1089.0 14 4508.0 15 12013.0 16 21976.0 17 30819.0 18 39423.0 19 52035.0 20 69938.0 21 96767.0 22 143829.0 23 222679.0 24 345612.0 25 439410.0 26 447823.0 27 412419.0 28 406840.0 29 454426.0 30 536810.0 31 654782.0 32 806242.0 33 1023474.0 34 1367513.0 35 2013560.0 36 3401040.0 37 6481266.0 38 8216398.0 39 2313690.0 40 4570.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.34497151708538 33.51060733254589 27.23113941727705 20.913281733091683 2 21.700725578183043 31.16784421132175 28.045644465890952 19.085785744604262 3 24.010386871114285 26.984748496339066 27.087312920013208 21.91755171253344 4 20.34697777009209 27.468347381179946 26.992615521367263 25.1920593273607 5 21.191339971752537 31.48686109234561 26.573087604409473 20.748711331492384 6 25.5486700397841 26.686334451281336 26.896562543048812 20.868432965885756 7 20.91631457122249 27.148107504195966 31.248512859155337 20.687065065426204 8 24.938879010883806 26.60928956434134 27.25252044560535 21.199310979169503 9 25.126738372723523 26.356399015509428 27.378825832192693 21.13803677957435 10-11 20.61379881519006 29.263131536924924 29.259556070638826 20.86351357724619 12-13 20.722263507635343 26.947684883081752 29.030196652977196 23.299854956305705 14-15 20.71366373683822 31.685692299534747 26.614370490116322 20.986273473510707 16-17 23.196789267912372 26.86155061794449 26.595700460990347 23.345959653152793 18-19 20.846872691901677 29.224785717964906 26.536288478561037 23.392053111572373 20-21 23.23846010009712 29.221759614314678 26.509929709102643 21.029850576485558 22-23 23.234712192116884 26.893571608046564 28.884656689955545 20.98705950988101 24-25 20.974599159110145 29.221209734367477 26.350481174270158 23.453709932252224 26-27 21.020499641978013 26.84354509965369 31.08551816677421 21.05043709159409 28-29 21.013999574208096 26.93026218709424 31.054716591256025 21.00102164744164 30-31 21.046618418244357 26.913983573705032 31.001642482947673 21.037755525102938 32-33 21.060685471676447 26.93593430547825 30.949001360775508 21.0543788620698 34-35 21.106891753732405 26.957035718292026 30.92214456301307 21.013927964962495 36-37 21.137402288351716 26.937867755109433 30.850195589829703 21.07453436670915 38-39 21.16733328768105 26.91776720640276 30.83787879958657 21.07702070632962 40-41 21.188085921619624 27.016590296120217 30.806188111069986 20.989135671190166 42-43 21.216368759379375 27.034607560202883 30.721439000594742 21.027584679823004 44-45 21.25594854495704 27.05300876502847 30.673862072305873 21.01718061770862 46-47 21.306663658270136 27.142726431967773 30.585636745178757 20.964973164583338 48-49 21.3477486471009 27.16919955632873 30.470571289790037 21.012480506780328 50-51 21.339568511329716 27.23260141800961 30.409462332471005 21.01836773818967 52-53 21.42689727161663 27.376230863412964 30.183588665464946 21.013283199505455 54-55 21.71708780291255 27.562809892375384 29.566957736273437 21.153144568438634 56-57 21.991849771417332 27.957268129817127 28.674908311552155 21.37597378721339 58-59 22.083728960695634 28.373982532133248 28.141841918430444 21.400446588740678 60-61 22.128551514245874 28.584584487765053 27.80412644609658 21.482737551892495 62-63 22.123476159824502 28.714397377403174 27.65505255099074 21.50707391178159 64-65 22.088254343075437 28.80865807835687 27.575575756745106 21.527511821822586 66-67 22.083744289872513 28.688344965849353 27.6092134089918 21.61869733528633 68-69 22.032516806325177 28.574773764738243 27.733891706469276 21.658817722467305 70-71 21.97788527388178 28.528814706655414 27.807991362090316 21.68530865737249 72-73 21.945790058187736 28.484570229746314 27.800310361677504 21.769329350388446 74-75 21.937659431027555 28.525012171273612 27.705164440343072 21.83216395735576 76-77 21.9768575533619 28.610285276111984 27.526212835280873 21.886644335245244 78-79 22.039183120064973 28.719253867203175 27.21177445125394 22.029788561477908 80-81 22.074042189652037 28.858033956439456 26.912533457709962 22.15539039619854 82-83 22.06241185362755 29.01140627924438 26.66326402779953 22.262917839328537 84-85 22.085743630969624 29.072001110335805 26.443728405453225 22.39852685324134 86-87 22.10688684871589 29.061917799513587 26.311171556185524 22.520023795584994 88-89 22.179751091807272 29.101283511924425 26.13773679875774 22.581228597510563 90-91 22.191601110847028 29.061923954533086 26.012009300215368 22.734465634404515 92-93 22.22348905576562 29.026706610894237 25.959864148590185 22.789940184749963 94-95 22.26145678796957 29.045374502998023 25.903734806244127 22.78943390278827 96-97 22.29830806365379 28.988446334353313 25.822176335707468 22.89106926628543 98-99 22.285263487884187 28.92388965332685 25.840700825221603 22.95014603356736 100 22.2755986034957 28.93227178623815 25.790862028854516 23.00126758141164 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 49839.0 1 31669.5 2 10714.0 3 7347.5 4 6773.0 5 6943.0 6 7110.0 7 7218.0 8 7460.5 9 7746.0 10 8484.0 11 10849.5 12 87852.5 13 107363.5 14 50256.0 15 52564.5 16 60606.5 17 69142.5 18 77081.5 19 85083.5 20 95659.0 21 112259.0 22 135666.0 23 162735.5 24 191598.0 25 223894.5 26 260399.0 27 303869.0 28 359026.5 29 416346.0 30 463918.5 31 507750.0 32 554772.5 33 599000.5 34 637307.5 35 682876.0 36 737089.0 37 786514.0 38 838914.0 39 879520.5 40 901637.0 41 935371.0 42 980458.0 43 1002758.5 44 1011943.5 45 1035866.5 46 1059128.0 47 1070033.5 48 1067805.5 49 1058831.0 50 1052582.0 51 1047228.5 52 1030302.0 53 981217.0 54 910686.5 55 842569.5 56 770523.5 57 691937.0 58 607159.5 59 514594.5 60 419905.0 61 336344.5 62 262585.0 63 194710.5 64 142271.5 65 103666.0 66 74757.0 67 54982.0 68 41171.5 69 31504.5 70 24580.5 71 19497.0 72 16078.5 73 13162.5 74 10370.0 75 8689.5 76 6970.5 77 4600.5 78 2915.0 79 1957.5 80 1346.5 81 988.5 82 680.5 83 443.5 84 323.5 85 223.0 86 137.0 87 88.5 88 52.5 89 32.0 90 23.0 91 18.5 92 12.0 93 10.0 94 9.5 95 7.5 96 4.0 97 2.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1055486973512173 2 0.0 3 0.0 4 0.0 5 0.009835446616539751 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.16252700834291E-5 20-21 0.011032819816216703 22-23 0.0 24-25 8.876215791763778E-4 26-27 0.004857771381721377 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.007424046904255708 42-43 0.03905701481505364 44-45 0.015087901514705408 46-47 0.004722879546987256 48-49 0.0015837300596561637 50-51 0.0 52-53 0.0013855556357875165 54-55 7.827057077165057E-5 56-57 7.843710390095196E-4 58-59 3.996795103233221E-5 60-61 2.814409885193393E-4 62-63 2.464690313660486E-4 64-65 8.826255852973363E-5 66-67 3.2807026472372685E-4 68-69 5.545553205736094E-4 70-71 4.1799815454647435E-4 72-73 0.0012489984697603815 74-75 0.001642016654911648 76-77 0.0024630249823674724 78-79 0.004521374460532582 80-81 0.004221614827790089 82-83 0.007523966781836537 84-85 0.0023531131170285587 86-87 0.002796091240970241 88-89 0.005667122390126104 90-91 0.005797018230981184 92-93 0.005647138414609938 94-95 0.002614570130031732 96-97 0.005593847813233495 98-99 0.007868690359490404 100 0.29937327588251234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0024056E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.64574556531432 #Duplication Level Percentage of deduplicated Percentage of total 1 67.4019084610148 26.721989134619996 2 16.208442454818883 12.851915711475764 3 5.978334876879489 7.110466302990269 4 2.993824713572987 4.747696514458587 5 1.7310527422472677 3.4314438289637406 6 1.082710726757536 2.57549243963195 7 0.8351550520208596 2.3177241290004096 8 0.5619335625286275 1.7822620035696488 9 0.4374620647021804 1.5609158740493745 >10 2.4532107221511104 17.728949197297247 >50 0.18073461399301316 4.992321108895182 >100 0.13428800127905954 9.200937253124737 >500 4.391339518869383E-4 0.11028963969460681 >1k 4.611840528414138E-4 0.33391092099791153 >5k 0.0 0.0 >10k+ 4.169002943591845E-5 4.5336859412305595 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1229235 4.09416702393574 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72452 0.24131316568287772 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0027777725967470883 9.991987758083052E-6 0.0 0.0 2.664530068822147E-5 2 0.0028477165110536696 1.9983975516166105E-5 0.0 3.3306625860276837E-6 2.664530068822147E-5 3 0.002940975063462445 2.331463810219379E-5 0.0 1.665331293013842E-5 3.663728844630452E-5 4 0.003020910965527109 2.9975963274249154E-5 0.0 1.665331293013842E-5 4.329861361835989E-5 5 0.0031008468675917736 3.330662586027684E-5 0.0 1.665331293013842E-5 4.662927620438758E-5 6 0.0031941054200005487 3.663728844630452E-5 0.0 2.331463810219379E-5 4.995993879041526E-5 7 0.0032207507206887705 3.663728844630452E-5 0.0 2.664530068822147E-5 5.662126396247063E-5 8 0.0032607186717211025 3.663728844630452E-5 0.0 2.664530068822147E-5 5.995192654849831E-5 9 0.0033672998744739883 3.996795103233221E-5 0.0 2.664530068822147E-5 5.995192654849831E-5 10-11 0.003467219752054819 4.496394491137374E-5 0.0 2.664530068822147E-5 6.994391430658136E-5 12-13 0.0035954502616168844 5.495593266945678E-5 0.0 2.664530068822147E-5 7.993590206466442E-5 14-15 0.0037486607405741584 7.493990818562289E-5 0.0 2.8310631981235314E-5 8.32665646506921E-5 16-17 0.003941839170563764 7.660523947863673E-5 0.0 2.9975963274249154E-5 9.658921499480284E-5 18-19 0.004158332238655563 8.992788982274748E-5 0.0 2.9975963274249154E-5 9.658921499480284E-5 20-21 0.004349845337352155 1.0824653404589973E-4 0.0 3.330662586027684E-5 9.991987758083052E-5 22-23 0.004617963675527384 1.1990385309699662E-4 0.0 3.663728844630452E-5 1.0658120275288588E-4 24-25 0.005538891880564039 1.3988782861316273E-4 0.0 3.663728844630452E-5 1.0991186533891356E-4 26-27 0.00804021948267083 1.548758102502873E-4 0.0 3.663728844630452E-5 1.1490785921795509E-4 28-29 0.01463826206559167 1.781904483524811E-4 0.0 3.663728844630452E-5 1.1990385309699662E-4 30-31 0.030079213814416014 2.0483574904070256E-4 0.0 3.663728844630452E-5 1.315611721480935E-4 32-33 0.06183874690348299 2.1149707421275793E-4 0.0 4.329861361835989E-5 1.382224973201489E-4 34-35 0.11698452734034336 2.3148104972892404E-4 0.0 4.662927620438758E-5 1.432184911991904E-4 36-37 0.19992801772019078 2.581263504171455E-4 0.0 4.662927620438758E-5 1.4987981637124578E-4 38-39 0.31571017586697814 2.5979168171015934E-4 1.6653312930138419E-6 4.662927620438758E-5 1.5321047895727345E-4 40-41 0.46836443417238494 2.664530068822147E-4 3.3306625860276837E-6 4.995993879041526E-5 1.5654114154330115E-4 42-43 0.6586701676815417 2.7977565722632546E-4 3.3306625860276837E-6 4.995993879041526E-5 1.632024667153565E-4 44-45 0.8889988081557002 3.0475562662153307E-4 3.3306625860276837E-6 5.662126396247063E-5 1.6486779800837034E-4 46-47 1.1615302742574154 3.197436082586577E-4 3.3306625860276837E-6 5.662126396247063E-5 1.7152912318042573E-4 48-49 1.4772038128359473 3.2807026472372685E-4 3.3306625860276837E-6 6.161725784151214E-5 1.7652511705946726E-4 50-51 1.83460722295482 3.447235776538653E-4 3.3306625860276837E-6 7.327457689260904E-5 1.8318644223152262E-4 52-53 2.2315555899575994 3.563808967049622E-4 3.3306625860276837E-6 7.493990818562289E-5 1.8818243611056415E-4 54-55 2.668834950214588 3.7803020351414213E-4 3.3306625860276837E-6 7.660523947863673E-5 1.8984776740357798E-4 56-57 3.145497730220061 3.9801417903030824E-4 3.3306625860276837E-6 7.660523947863673E-5 1.981744238686472E-4 58-59 3.658897718549419 4.263248110115436E-4 3.3306625860276837E-6 7.993590206466442E-5 2.1149707421275793E-4 60-61 4.204646767245571 4.4464345523469585E-4 3.3306625860276837E-6 8.659722723671978E-5 2.1649306809179945E-4 62-63 4.783679127163898 4.6629276204387574E-4 3.3306625860276837E-6 9.325855240877516E-5 2.2315439326385482E-4 64-65 5.394377761618883 4.7461941850894496E-4 3.3306625860276837E-6 9.658921499480284E-5 2.2815038714289634E-4 66-67 6.034301294934968 4.912727314390834E-4 3.3306625860276837E-6 1.0658120275288588E-4 2.331463810219379E-4 68-69 6.702618726796939 4.995993879041526E-4 3.3306625860276837E-6 1.0991186533891357E-4 2.448037000730348E-4 70-71 7.3969719480938885 5.16252700834291E-4 3.3306625860276837E-6 1.1324252792494125E-4 2.4813436265906246E-4 72-73 8.115507445096693 5.245793572993602E-4 3.3306625860276837E-6 1.1657319051096894E-4 2.5313035653810396E-4 74-75 8.859913863736466 5.428980015225125E-4 3.3306625860276837E-6 1.1823852180398278E-4 2.63122344296187E-4 76-77 9.627121998440185 5.562206518666232E-4 3.3306625860276837E-6 1.2156918439001046E-4 2.6478767558920085E-4 78-79 10.4121375206601 5.762046273827892E-4 3.3306625860276837E-6 1.2823050956206584E-4 2.7144900076125623E-4 80-81 11.219520107476484 5.945232716059415E-4 3.3306625860276837E-6 1.3655716602713503E-4 2.781103259333116E-4 82-83 12.039249460499274 6.195032410011492E-4 3.3306625860276837E-6 1.382224973201489E-4 2.7977565722632546E-4 84-85 12.874208268196675 6.244992348801907E-4 3.3306625860276837E-6 1.4321849119919042E-4 2.7977565722632546E-4 86-87 13.727800467731608 6.461485416893707E-4 3.3306625860276837E-6 1.465491537852181E-4 2.8477165110536696E-4 88 14.376708463373502 6.494792042753984E-4 3.3306625860276837E-6 1.465491537852181E-4 2.9642897015646385E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 154510 0.0 85.868126 8 AGTGGTA 157935 0.0 84.437355 5 GTATCAA 157715 0.0 83.97121 9 TGGTATC 158325 0.0 83.91184 7 GTGGTAT 159230 0.0 83.70642 6 GCAGTGG 167525 0.0 79.631775 3 AAGCAGT 166065 0.0 78.63463 1 CAGTGGT 170650 0.0 77.989006 4 AGCAGTG 176320 0.0 75.611694 2 CAACGCA 150370 0.0 44.988045 12-13 ATCAACG 152135 0.0 44.512444 10-11 TCAACGC 150325 0.0 44.073067 12-13 ACGCAGA 153885 0.0 44.070168 14-15 AACGCAG 151990 0.0 43.63468 14-15 AGAGTAC 156540 0.0 43.134304 18-19 GAGTACT 152780 0.0 42.925793 20-21 CGCAGAG 155455 0.0 42.81024 16-17 TATCAAC 157860 0.0 41.985737 10-11 AGTACTT 164030 0.0 40.913002 20-21 GTACTTT 163910 0.0 40.03147 22-23 >>END_MODULE