FastQCFastQC Report
Thu 26 May 2016
SRR1660754_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660754_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29913037
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13171874.40338772689647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA924230.3089723052861533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1669250.085.375958
AGTGGTA1700050.083.9566965
TGGTATC1703850.083.501637
GTGGTAT1711750.083.37426
GTATCAA1708150.083.316189
AAGCAGT1781350.080.446791
GCAGTGG1786500.080.3525853
CAGTGGT1794350.079.384774
AGCAGTG1874600.076.6335752
CAACGCA1638500.044.56554812-13
ATCAACG1657300.044.19179510-11
ACGCAGA1660650.043.92321414-15
TCAACGC1636750.043.65613612-13
AACGCAG1650400.043.34898414-15
AGAGTAC1670650.043.1790618-19
GAGTACT1627000.042.877920-21
CGCAGAG1680650.042.46496216-17
TATCAAC1711450.041.59148410-11
AGTACTT1726250.041.36071820-21
GTACTTT1728500.040.3885122-23
Produced by FastQC (version 0.11.5)