##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660754_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29913037 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71626217692306 33.0 31.0 34.0 30.0 34.0 2 31.870195293109155 34.0 31.0 34.0 30.0 34.0 3 31.911700072446674 34.0 31.0 34.0 30.0 34.0 4 35.337046552645255 37.0 35.0 37.0 33.0 37.0 5 35.333524442870846 37.0 35.0 37.0 33.0 37.0 6 35.33785235514535 37.0 35.0 37.0 32.0 37.0 7 35.33171332619954 37.0 35.0 37.0 33.0 37.0 8 35.326015409267875 37.0 35.0 37.0 32.0 37.0 9 36.94228091918584 39.0 37.0 39.0 33.0 39.0 10-11 36.895923707111386 39.0 37.0 39.0 33.0 39.0 12-13 36.90816383839595 39.0 37.0 39.0 33.0 39.0 14-15 38.19151285106892 40.0 38.0 41.0 33.0 41.0 16-17 38.159767411781026 40.0 38.0 41.0 33.0 41.0 18-19 38.120090865397586 40.0 38.0 41.0 33.0 41.0 20-21 38.082802792641886 40.0 38.0 41.0 33.0 41.0 22-23 37.96152070750957 40.0 38.0 41.0 32.5 41.0 24-25 37.88158840240796 40.0 38.0 41.0 32.0 41.0 26-27 37.840163538058675 40.0 38.0 41.0 32.0 41.0 28-29 37.74700328823182 40.0 38.0 41.0 32.0 41.0 30-31 37.624386116327805 40.0 37.0 41.0 31.5 41.0 32-33 37.49371198919053 40.0 37.0 41.0 31.0 41.0 34-35 37.35747918207035 40.0 37.0 41.0 31.0 41.0 36-37 37.16107642296568 40.0 37.0 41.0 30.0 41.0 38-39 36.75585230613662 39.0 35.0 41.0 30.0 41.0 40-41 36.73716157607133 39.0 35.0 41.0 30.0 41.0 42-43 36.759480590352624 39.0 35.0 41.0 30.0 41.0 44-45 36.6984228983503 39.0 35.0 41.0 30.0 41.0 46-47 36.82369625324236 40.0 35.0 41.0 30.0 41.0 48-49 36.76400861604256 40.0 35.0 41.0 30.0 41.0 50-51 35.65074614456566 38.5 34.0 40.0 28.0 40.5 52-53 35.704894340885545 38.5 35.0 39.5 28.0 40.5 54-55 36.02739427628161 39.0 35.0 41.0 27.5 41.0 56-57 35.74466203481779 39.0 35.0 41.0 26.0 41.0 58-59 35.45879473889595 39.0 35.0 41.0 25.0 41.0 60-61 35.23127245488313 39.0 34.5 41.0 25.0 41.0 62-63 34.97456642399767 38.0 34.0 40.5 24.0 41.0 64-65 34.69884986937301 37.5 34.0 40.0 23.5 41.0 66-67 34.41576310021614 37.0 34.0 40.0 23.0 41.0 68-69 34.117538817606516 36.5 33.5 39.5 23.0 41.0 70-71 33.79404025408721 36.0 33.0 39.0 23.0 41.0 72-73 33.454535793206155 35.5 33.0 39.0 23.0 41.0 74-75 33.10452352263663 35.0 33.0 38.0 22.0 40.0 76-77 32.72940069575684 35.0 32.5 37.0 21.5 39.5 78-79 32.381989632146016 35.0 32.0 37.0 20.0 39.0 80-81 32.05792546239955 35.0 32.0 36.0 20.0 39.0 82-83 31.75286439487906 35.0 32.0 36.0 20.0 37.0 84-85 31.48847260142793 35.0 31.0 35.0 19.5 37.0 86-87 31.236996547692563 35.0 31.0 35.0 19.0 36.5 88-89 31.04786439772063 35.0 31.0 35.0 18.5 36.0 90-91 30.8774674233178 35.0 31.0 35.0 18.0 36.0 92-93 30.714031577602768 35.0 31.0 35.0 18.0 36.0 94-95 30.553115201910124 34.0 30.5 35.0 17.0 35.0 96-97 30.43777138041851 34.0 30.0 35.0 17.0 35.0 98-99 30.336174140392366 34.0 30.0 35.0 16.5 35.0 100 30.216703238791837 34.0 30.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6516705925960906 1101 2 0.6350927415239092 1101 3 0.630480434421969 1101 4 0.5411258115542665 1101 5 0.5730507725232457 1101 6 0.5749067512003521 1101 7 0.5111831270882874 1101 8 0.5875191225951184 1101 9 0.7151163576103485 1101 10-11 0.6730309982132283 1101 12-13 0.6387811369226384 1101 14-15 0.7873473554134591 1101 16-17 0.8098274341105807 1101 18-19 0.7963297910107485 1101 20-21 0.7448583425125577 1101 22-23 0.7182740988694789 1101 24-25 0.644582587348026 1101 26-27 0.6433483188459732 1101 28-29 0.6385930075474775 1101 30-31 0.6631381536171119 1101 32-33 0.6619268810337715 1101 34-35 0.6287589103060824 1101 36-37 0.6714450248977428 1101 38-39 0.7047798631666922 1101 40-41 0.6871650776642397 1101 42-43 0.6173925746383659 1101 44-45 0.5746626645388275 1101 46-47 0.6414942260702929 1101 48-49 0.6867481478075774 1101 50-51 0.6810010900706445 1101 52-53 0.7228543890959429 1101 54-55 0.774985850769383 1101 56-57 0.7148632429237338 1101 58-59 0.7105354216764894 1101 60-61 0.6730926017360233 1101 62-63 0.6385578488447194 1101 64-65 0.5980431707025531 1101 66-67 0.5774740806014478 1101 68-69 0.5561978143309858 1101 70-71 0.5801398497897878 1101 72-73 0.5491695701113173 1101 74-75 0.49937665965025246 1101 76-77 0.4504513006773294 1101 78-79 0.4083249773944786 1101 80-81 0.3796803403195739 1101 82-83 0.34162578729595694 1101 84-85 0.30732113500563685 1101 86-87 0.21942419016265546 1101 88-89 0.24279069959910515 1101 90-91 0.23447045729803762 1101 92-93 0.2090987093228911 1101 94-95 0.23924566906434208 1101 96-97 0.16616299177189475 1101 98-99 0.14246119898255216 1101 100 0.14074461924782966 1102 1 0.6032819876304529 1102 2 0.5967708419298852 1102 3 0.5923412533066568 1102 4 0.5311387861902475 1102 5 0.5033908186784402 1102 6 0.531611588731181 1102 7 0.5470993922952374 1102 8 0.5487311247806019 1102 9 0.6865414811543076 1102 10-11 0.6123099234459559 1102 12-13 0.6092568510366831 1102 14-15 0.7592909410624884 1102 16-17 0.7558778973694302 1102 18-19 0.7603264072249232 1102 20-21 0.7187256162746181 1102 22-23 0.6660580104680278 1102 24-25 0.7060339003664495 1102 26-27 0.6751880989606676 1102 28-29 0.70949432766988 1102 30-31 0.7040584308415418 1102 32-33 0.7047338842694728 1102 34-35 0.6682551071280827 1102 36-37 0.694878318217782 1102 38-39 0.7518365154256941 1102 40-41 0.6827412894450546 1102 42-43 0.6536976352662407 1102 44-45 0.6390622009693274 1102 46-47 0.6922080975053859 1102 48-49 0.7349397100922843 1102 50-51 0.6841041563672761 1102 52-53 0.7307940048597956 1102 54-55 0.7971092894035863 1102 56-57 0.7405993433219891 1102 58-59 0.7280705549281166 1102 60-61 0.738782703071827 1102 62-63 0.7208342595965433 1102 64-65 0.6771539153462882 1102 66-67 0.6209552878990294 1102 68-69 0.63970432574542 1102 70-71 0.6418792447197248 1102 72-73 0.6109358044165205 1102 74-75 0.5575401568172325 1102 76-77 0.5420813567076408 1102 78-79 0.4972050151744938 1102 80-81 0.49728076711637215 1102 82-83 0.43806551205653577 1102 84-85 0.3859917304396028 1102 86-87 0.41282009248210016 1102 88-89 0.3829538216983899 1102 90-91 0.3166211002481276 1102 92-93 0.31387255317984497 1102 94-95 0.30091722431128787 1102 96-97 0.26845637143518175 1102 98-99 0.22887831645124024 1102 100 0.23557896230898123 1103 1 0.5747403889284115 1103 2 0.5429141486030709 1103 3 0.5337640518870259 1103 4 0.49974582748755836 1103 5 0.4724001193535159 1103 6 0.4941284679550151 1103 7 0.4814503076683394 1103 8 0.5021028390660902 1103 9 0.6009310565667647 1103 10-11 0.5606840505229158 1103 12-13 0.5836500624351686 1103 14-15 0.7188010298991969 1103 16-17 0.6719233874545978 1103 18-19 0.6828872992857669 1103 20-21 0.6333432751304358 1103 22-23 0.6329148122437758 1103 24-25 0.635523244689054 1103 26-27 0.6583407509367092 1103 28-29 0.6548238338113705 1103 30-31 0.6242420880520143 1103 32-33 0.6348514897106057 1103 34-35 0.6326044878896511 1103 36-37 0.637720036377246 1103 38-39 0.6813720130173024 1103 40-41 0.6069147996272406 1103 42-43 0.602142593399094 1103 44-45 0.5950111755510576 1103 46-47 0.6527260575868468 1103 48-49 0.6060671762969108 1103 50-51 0.5499335258464271 1103 52-53 0.6199552730151936 1103 54-55 0.6594376829302462 1103 56-57 0.6117352017736692 1103 58-59 0.6529130134948034 1103 60-61 0.6000955876727758 1103 62-63 0.5639910330165563 1103 64-65 0.5336313851423782 1103 66-67 0.4759243289937274 1103 68-69 0.49062373955710825 1103 70-71 0.4908834635047725 1103 72-73 0.5005657358089621 1103 74-75 0.448464692517085 1103 76-77 0.4492353653321288 1103 78-79 0.43742049985921483 1103 80-81 0.3813720240345404 1103 82-83 0.31724839437877606 1103 84-85 0.34003263645203674 1103 86-87 0.3010253254235806 1103 88-89 0.311056197918564 1103 90-91 0.29941400211338376 1103 92-93 0.2652454730615226 1103 94-95 0.2912319291178207 1103 96-97 0.269647027620195 1103 98-99 0.1847495441438589 1103 100 0.20108984519459483 1104 1 0.5199572991271211 1104 2 0.5184305522134203 1104 3 0.47610116880512265 1104 4 0.4446918244794631 1104 5 0.4459024085173837 1104 6 0.4591253609619841 1104 7 0.4077391072844918 1104 8 0.4614352297982407 1104 9 0.5712195850070287 1104 10-11 0.545341407878503 1104 12-13 0.5386063598873463 1104 14-15 0.6619415857168391 1104 16-17 0.6726622716503456 1104 18-19 0.656076423176934 1104 20-21 0.5829345250737461 1104 22-23 0.5721186471909121 1104 24-25 0.6008818906982469 1104 26-27 0.6308280544884965 1104 28-29 0.6148389244920622 1104 30-31 0.622113537002889 1104 32-33 0.6104273373372493 1104 34-35 0.612525755061121 1104 36-37 0.5860734011838034 1104 38-39 0.6232721856951144 1104 40-41 0.5706309724146834 1104 42-43 0.5494148078662491 1104 44-45 0.5458582790831059 1104 46-47 0.5713893590852308 1104 48-49 0.5825484433662851 1104 50-51 0.5733994931870399 1104 52-53 0.561870600928394 1104 54-55 0.6489631197246339 1104 56-57 0.6308708926319682 1104 58-59 0.6419312288102361 1104 60-61 0.6178953549421777 1104 62-63 0.5662867779295198 1104 64-65 0.5459359326107105 1104 66-67 0.5622453758755341 1104 68-69 0.5370135435027237 1104 70-71 0.5216368919342287 1104 72-73 0.5135332708486402 1104 74-75 0.4702129787402569 1104 76-77 0.48813940300692593 1104 78-79 0.4378462738805595 1104 80-81 0.42662826057951264 1104 82-83 0.4333709479243275 1104 84-85 0.44281862449556897 1104 86-87 0.42361195621515435 1104 88-89 0.4454634433406781 1104 90-91 0.414830953397999 1104 92-93 0.3665023660860669 1104 94-95 0.3408947468813359 1104 96-97 0.34194520395699257 1104 98-99 0.29332052098612493 1104 100 0.2462887077535285 1105 1 0.39745271723326425 1105 2 0.37782355446037386 1105 3 0.3842534677584979 1105 4 0.32009515416655177 1105 5 0.3341931806218099 1105 6 0.353381410410492 1105 7 0.3170043742547648 1105 8 0.3766218944989248 1105 9 0.4139805043859468 1105 10-11 0.4217763007719455 1105 12-13 0.43260896033749674 1105 14-15 0.5308847930921559 1105 16-17 0.48188126421231203 1105 18-19 0.5127505741048495 1105 20-21 0.4681784267914466 1105 22-23 0.44137366777489717 1105 24-25 0.4370843098943169 1105 26-27 0.4118903998313641 1105 28-29 0.39950489673336875 1105 30-31 0.436521894287786 1105 32-33 0.4238842180292437 1105 34-35 0.40808206635640687 1105 36-37 0.3853276117323716 1105 38-39 0.4278163410234015 1105 40-41 0.38189966805816056 1105 42-43 0.38098220764639734 1105 44-45 0.3477934725793901 1105 46-47 0.41535401941484196 1105 48-49 0.43679698882949936 1105 50-51 0.34214858145400484 1105 52-53 0.3800032477608042 1105 54-55 0.4937878051502764 1105 56-57 0.5138411870139095 1105 58-59 0.5344830805698209 1105 60-61 0.5211697406407083 1105 62-63 0.4668433232661755 1105 64-65 0.40067426586013966 1105 66-67 0.40636804258190296 1105 68-69 0.42990552006295957 1105 70-71 0.4569882957707918 1105 72-73 0.425800314604885 1105 74-75 0.4300786487408246 1105 76-77 0.4077848697428621 1105 78-79 0.3758442000816018 1105 80-81 0.40475737490718444 1105 82-83 0.3951297460204408 1105 84-85 0.3666620986584057 1105 86-87 0.3717632504639603 1105 88-89 0.3824481143624361 1105 90-91 0.30672320052342883 1105 92-93 0.2854851228484456 1105 94-95 0.3066463891878435 1105 96-97 0.28773971568660883 1105 98-99 0.318884215790316 1105 100 0.3208634737149687 1106 1 0.35489621660369153 1106 2 0.33527042868236023 1106 3 0.30070796959015667 1106 4 0.3321316499311422 1106 5 0.3317764005783559 1106 6 0.342205782659434 1106 7 0.30789726962063213 1106 8 0.33866980153582915 1106 9 0.41658749616959767 1106 10-11 0.40030927136364625 1106 12-13 0.4034152921154117 1106 14-15 0.4186752785687844 1106 16-17 0.42783819987868554 1106 18-19 0.41651426361983823 1106 20-21 0.4056506276313172 1106 22-23 0.3607508349020989 1106 24-25 0.37610160658691427 1106 26-27 0.40340067155674575 1106 28-29 0.4128689108939696 1106 30-31 0.3936994736733084 1106 32-33 0.34509080004085746 1106 34-35 0.2974318448203235 1106 36-37 0.3249460960434405 1106 38-39 0.32762371759652353 1106 40-41 0.3269653947964102 1106 42-43 0.2958109829820117 1106 44-45 0.28566113441664953 1106 46-47 0.3308289517107994 1106 48-49 0.32248526428691804 1106 50-51 0.30190065857549797 1106 52-53 0.34484386166490566 1106 54-55 0.3647388410155017 1106 56-57 0.3889172384332866 1106 58-59 0.4361477134425158 1106 60-61 0.4104153200078642 1106 62-63 0.40986884455637096 1106 64-65 0.33166066421924256 1106 66-67 0.3717211759857406 1106 68-69 0.3920091021591503 1106 70-71 0.39638037194861653 1106 72-73 0.40820017567379807 1106 74-75 0.3938152310262879 1106 76-77 0.3854895762734287 1106 78-79 0.3289396683785597 1106 80-81 0.29988278606165153 1106 82-83 0.3265422421461146 1106 84-85 0.3198626969116809 1106 86-87 0.2837032733215423 1106 88-89 0.2615152075003593 1106 90-91 0.30428595544676895 1106 92-93 0.30250621385333787 1106 94-95 0.3184457289161209 1106 96-97 0.27171777168687683 1106 98-99 0.22268150506092255 1106 100 0.31850343558354055 1107 1 0.43753958234437107 1107 2 0.4371032862938442 1107 3 0.3595364256755289 1107 4 0.39928509995818473 1107 5 0.4414286645083152 1107 6 0.4477941580144318 1107 7 0.3908955943704626 1107 8 0.4206174719078106 1107 9 0.527316068564545 1107 10-11 0.49050692099834947 1107 12-13 0.46507760771727646 1107 14-15 0.5591304771581491 1107 16-17 0.6210982480060352 1107 18-19 0.5786381804186433 1107 20-21 0.5451986093831707 1107 22-23 0.5187031194916969 1107 24-25 0.5565737490639862 1107 26-27 0.5681192848591721 1107 28-29 0.5448089935553 1107 30-31 0.5323854055257229 1107 32-33 0.5491923220155499 1107 34-35 0.5447869694440541 1107 36-37 0.5194756409679968 1107 38-39 0.5787478432269779 1107 40-41 0.5722716535518586 1107 42-43 0.5714299062619688 1107 44-45 0.5395618068655565 1107 46-47 0.5543367908977004 1107 48-49 0.5410495917560496 1107 50-51 0.49792351620080666 1107 52-53 0.566634392242797 1107 54-55 0.5894824882091285 1107 56-57 0.5799165488979483 1107 58-59 0.6435219235997067 1107 60-61 0.5904630797654917 1107 62-63 0.5593606537576434 1107 64-65 0.5314720431074775 1107 66-67 0.5005653487483173 1107 68-69 0.5594967898065448 1107 70-71 0.5595221913845805 1107 72-73 0.5393477090467016 1107 74-75 0.5597017603739829 1107 76-77 0.5436832898843988 1107 78-79 0.5535325912594331 1107 80-81 0.5532099681028271 1107 82-83 0.46799950182002803 1107 84-85 0.46397268409713277 1107 86-87 0.4887688651362616 1107 88-89 0.5432119193111511 1107 90-91 0.5390239515980007 1107 92-93 0.48783442905767416 1107 94-95 0.49814772186649137 1107 96-97 0.5363238332928724 1107 98-99 0.5268734938646098 1107 100 0.5432019648277091 1108 1 0.20542250636464487 1108 2 0.15537485655749705 1108 3 0.19737891915295336 1108 4 0.20118192470904717 1108 5 0.20968580842848183 1108 6 0.23496116398676747 1108 7 0.22750523085399266 1108 8 0.23821177976737573 1108 9 0.2900069841254833 1108 10-11 0.2582745970471052 1108 12-13 0.23622032272740512 1108 14-15 0.3044476330043224 1108 16-17 0.2948992961051573 1108 18-19 0.26690748773958717 1108 20-21 0.2522011823155239 1108 22-23 0.2376633847020102 1108 24-25 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-1.7829223320119283 2316 76-77 -1.7077292171642746 2316 78-79 -1.6030108111443226 2316 80-81 -1.5931249211491654 2316 82-83 -1.8118445703655652 2316 84-85 -1.529422588039214 2316 86-87 -1.4065284074987225 2316 88-89 -1.5773399821982643 2316 90-91 -1.4699994901579494 2316 92-93 -1.3689785940126455 2316 94-95 -1.3440242460478764 2316 96-97 -1.2335956190548067 2316 98-99 -1.2461557277252666 2316 100 -1.459571349493892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 19.0 4 78.0 5 161.0 6 1570.0 7 17985.0 8 25849.0 9 33189.0 10 35965.0 11 36567.0 12 39992.0 13 47103.0 14 58372.0 15 75118.0 16 92909.0 17 112135.0 18 123531.0 19 137099.0 20 154992.0 21 187796.0 22 240477.0 23 332295.0 24 453752.0 25 517838.0 26 499890.0 27 449877.0 28 434867.0 29 465828.0 30 539802.0 31 646622.0 32 798018.0 33 1021707.0 34 1379428.0 35 2024833.0 36 3317278.0 37 5924568.0 38 7398485.0 39 2280904.0 40 6138.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.31908008442166 34.61460751094327 26.733015906472467 21.333296498162596 2 21.3158460057278 31.569892386833647 28.09214031522461 19.02212129221394 3 24.361141226881784 26.732145107238797 26.888342958474425 22.018370707404994 4 19.7728131733485 27.408091638545674 26.79645794160011 26.022637246505713 5 21.107128954867743 31.848824740536553 26.4112347040827 20.63281160051301 6 25.903992964672895 26.632518122449415 26.791268302178743 20.67222061069894 7 20.827798260671425 27.097716624360142 31.545011628207458 20.529473486760974 8 25.23551186059777 26.57634194749266 27.130040323220943 21.058105868688624 9 25.37038950608726 26.27994275539458 27.348837899675647 21.00082983884251 10-11 20.49551543317204 29.48065214648571 29.28301923502644 20.740813185315808 12-13 20.574880302150632 26.97307597790087 29.083873724982762 23.368169994965736 14-15 20.58387451598445 32.17397150279325 26.43175448885381 20.810399492368497 16-17 23.28524850218318 26.902636131530212 26.401271124693892 23.41084424159272 18-19 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31.024630403274635 20.805555002162084 44-45 21.06516373925324 27.18021403848662 30.96244018013035 20.792182042129788 46-47 21.074275928424438 27.26529121215295 30.912311772930366 20.74812108649225 48-49 21.169419558901314 27.267175692496476 30.780903564181237 20.78250118442097 50-51 21.22108658726431 27.306920539663253 30.70851296630287 20.763479906769565 52-53 21.347585075965103 27.427015420912404 30.487313579184523 20.73808592393797 54-55 21.711035450869836 27.624900384314277 29.831663180629715 20.832400984186172 56-57 22.091128973050264 28.012596272498918 28.946279814769433 20.94999493968139 58-59 22.27854143391579 28.439447834616523 28.373547283799883 20.908463447667806 60-61 22.44908929223782 28.66842444413734 27.999882860177816 20.882603403447025 62-63 22.583259527857184 28.775781403054218 27.84485963169535 20.79609943739325 64-65 22.743952503518155 28.838550994055108 27.766982597147717 20.650513905279023 66-67 22.880302385126797 28.693678799319795 27.845798749560245 20.580220065993164 68-69 23.007144314879604 28.546684675754065 27.948087054581176 20.498083954785155 70-71 23.131818700076078 28.457841851460575 28.044520308441196 20.365819140022154 72-73 23.31677265892961 28.378170373427437 28.008302044385204 20.296754923257748 74-75 23.51611970505028 28.403279344840094 27.899045960428655 20.181554989680976 76-77 23.796554692298905 28.486699591089838 27.62057799303727 20.096167723573984 78-79 24.0581653153303 28.53693245548936 27.339024967904102 20.065877261276242 80-81 24.289497708740036 28.60882515766281 27.082184726799785 20.01949240679736 82-83 24.469867575671188 28.706855138574717 26.877323452399605 19.945953833354494 84-85 24.634478519890006 28.679961571786638 26.75845892542783 19.927100982895528 86-87 24.821291659039847 28.627708955481605 26.64743635680843 19.903563028670117 88-89 24.977522082247194 28.640810124576976 26.53177022598237 19.84989756719346 90-91 25.15986972734117 28.572874040606866 26.409907650001557 19.857348582050406 92-93 25.26796539292783 28.54761021113862 26.390737228517615 19.793687167415932 94-95 25.41872431140797 28.591645741454098 26.292633770496 19.696996176641935 96-97 25.530621712228562 28.55841609446913 26.204544927729362 19.70641726557294 98-99 25.617197773404122 28.56332703150384 26.14504400198594 19.6744311931061 100 25.656875887006255 28.651468867805157 26.032909175810957 19.65874606937763 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 57033.0 1 35722.0 2 12192.0 3 9639.0 4 9223.0 5 9371.0 6 9926.0 7 10353.5 8 10553.0 9 10947.0 10 11808.5 11 14253.5 12 90059.5 13 111124.0 14 57567.5 15 60851.0 16 68806.5 17 76443.0 18 83918.5 19 91809.5 20 102510.0 21 119387.5 22 142887.5 23 169367.0 24 195790.5 25 226464.5 26 262371.0 27 303953.5 28 354661.5 29 407050.0 30 453163.5 31 501161.5 32 554757.0 33 604953.0 34 647751.0 35 699124.0 36 764809.5 37 818869.5 38 868343.0 39 908359.0 40 928620.5 41 956663.0 42 991008.5 43 1001023.5 44 995283.0 45 1009445.5 46 1027878.0 47 1035560.0 48 1035362.0 49 1027218.0 50 1012667.5 51 994936.0 52 975690.0 53 933101.0 54 872226.0 55 810712.5 56 742433.0 57 666196.0 58 588020.0 59 505275.0 60 416582.0 61 338317.5 62 269361.0 63 205000.5 64 153478.5 65 113364.5 66 83188.0 67 62720.0 68 48601.5 69 37703.5 70 29827.0 71 25056.0 72 21361.5 73 17311.5 74 13769.0 75 11553.5 76 9247.0 77 6401.0 78 4500.5 79 3395.5 80 2644.5 81 2136.5 82 1656.0 83 1306.5 84 1061.5 85 873.5 86 745.0 87 639.5 88 521.0 89 442.0 90 391.5 91 340.0 92 283.0 93 239.5 94 206.0 95 184.5 96 163.0 97 124.0 98 81.5 99 58.5 100 45.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047056405539832015 2 0.015117154436709317 3 0.005395640703416374 4 1.1700583929341578E-4 5 5.114826689112175E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.0029071939435639E-5 12-13 3.343023979811879E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0384130170400284 22-23 0.07584987107795173 24-25 0.031161998027816433 26-27 0.03587399032736128 28-29 0.040699645442219726 30-31 0.03401861201856569 32-33 0.03485102498953884 34-35 0.03563663562479463 36-37 0.033405167118270206 38-39 0.04360974781664596 40-41 0.064418400578985 42-43 0.07256869304176637 44-45 0.07545706576032384 46-47 0.04850059189911075 48-49 0.030704003742582205 50-51 0.02816664854190499 52-53 0.028360543932734076 54-55 0.029202985975646672 56-57 0.029396881366475763 58-59 0.0373165051746501 60-61 0.057301102525965514 62-63 0.08096971230303363 64-65 0.10923832307632288 66-67 0.08996411832071749 68-69 0.053182496982837286 70-71 0.04652820775102173 72-73 0.05047966209515938 74-75 0.048079370877654445 76-77 0.051054662219687026 78-79 0.044096157805708594 80-81 0.04237617196809538 82-83 0.04175102648387056 84-85 0.04911403679940622 86-87 0.05278467712923967 88-89 0.05878874819698181 90-91 0.05570815159958516 92-93 0.051258586682455545 94-95 0.05311897952722086 96-97 0.05501280261178429 98-99 0.0496138188843881 100 0.05154274372073955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.9913037E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.650296847923556 #Duplication Level Percentage of deduplicated Percentage of total 1 67.6573362588458 28.179481391207094 2 16.931091470500483 14.103699714113832 3 6.11567210306886 7.641586755521488 4 2.788672816114732 4.645962024116541 5 1.5572581083442183 3.243013124068629 6 1.0395542240215698 2.597864521200673 7 0.6973119093003332 2.0330273612565857 8 0.5156908667229865 1.7182942144620295 9 0.38814188742216693 1.454960234722192 >10 2.0066959125300587 14.726814688085707 >50 0.1621707927726527 4.849853873613222 >100 0.13927202286292703 9.43324743795679 >500 6.594846622069727E-4 0.16643081017139194 >1k 4.243041213273546E-4 0.3533907250163533 >5k 1.5946236640353484E-5 0.03540666225312297 >10k+ 3.1892473160647685E-5 4.816966462234257 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1317187 4.40338772689647 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92423 0.3089723052861533 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0076521818897893916 6.6860479596237585E-6 0.0 0.0 1.0029071939435639E-5 2 0.0076521818897893916 1.0029071939435639E-5 0.0 0.0 1.3372095919247517E-5 3 0.007655524913769204 2.0058143878871277E-5 0.0 0.0 1.3372095919247517E-5 4 0.007658867937749015 2.3401167858683156E-5 0.0 0.0 1.3372095919247517E-5 5 0.007658867937749015 2.6744191838495034E-5 0.0 0.0 1.3372095919247517E-5 6 0.007662210961728828 3.677326377793067E-5 0.0 1.3372095919247517E-5 1.3372095919247517E-5 7 0.007662210961728828 3.677326377793067E-5 0.0 1.6715119899059395E-5 2.0058143878871277E-5 8 0.007662210961728828 4.345931173755443E-5 0.0 1.6715119899059395E-5 2.3401167858683156E-5 9 0.007665553985708639 5.014535969717819E-5 0.0 2.0058143878871277E-5 2.6744191838495034E-5 10-11 0.007672240033668263 6.853199158614353E-5 0.0 2.1729655868777218E-5 2.6744191838495034E-5 12-13 0.007683940617597605 8.524711148520292E-5 0.0 3.510175178802474E-5 2.6744191838495034E-5 14-15 0.007709013297446193 1.0363374337416825E-4 0.0 4.513082372746037E-5 4.178779974764849E-5 16-17 0.007732414465304877 1.0697676735398014E-4 0.0 4.847384770727225E-5 4.345931173755443E-5 18-19 0.007792588896941491 1.1533432730350985E-4 0.0 5.014535969717819E-5 4.680233571736631E-5 20-21 0.007844405768628576 1.2369188725303953E-4 0.0 5.014535969717819E-5 5.850291964670789E-5 22-23 0.007999856383689828 1.303779352126633E-4 0.0 5.014535969717819E-5 7.020350357604946E-5 24-25 0.008748693755167687 1.4876456710162864E-4 0.0 5.348838367699007E-5 7.521803954576729E-5 26-27 0.011255961740026597 1.7383724695021774E-4 0.0 5.348838367699007E-5 8.691862347510886E-5 28-29 0.019005091325230535 2.055959747584306E-4 0.0 5.515989566689601E-5 9.026164745492074E-5 30-31 0.03759063314099467 2.273256306272078E-4 0.0 6.017443163661383E-5 1.0697676735398014E-4 32-33 0.07552726926389988 2.3902621455654938E-4 0.0 6.017443163661383E-5 1.2202037526313361E-4 34-35 0.1396113674449037 2.607558704253266E-4 0.0 6.017443163661383E-5 1.4040700715209893E-4 36-37 0.23431923679297426 2.674419183849503E-4 0.0 6.017443163661383E-5 1.5377910307134645E-4 38-39 0.3658989891263799 2.9585762221335133E-4 0.0 6.017443163661383E-5 1.5879363904106427E-4 40-41 0.5370902325965765 3.226018140518464E-4 0.0 6.518896760633165E-5 1.7550875894012366E-4 42-43 0.7488156418219922 3.4934600589034144E-4 0.0 7.020350357604946E-5 1.8219480689974743E-4 44-45 1.0033066853091515 3.660611257894008E-4 0.0 7.354652755586135E-5 1.93895390829089E-4 46-47 1.3003260083554873 3.794332217086483E-4 0.0 7.688955153567323E-5 2.1729655868777217E-4 48-49 1.6403967942138407 4.0283438956733144E-4 0.0 7.856106352557916E-5 2.4069772654645533E-4 50-51 2.021712138423123 4.1453497349667307E-4 0.0 9.026164745492074E-5 2.557413344556088E-4 52-53 2.442978290703147 4.3459311737554433E-4 0.0 1.0530525536407419E-4 2.6409889440513843E-4 54-55 2.90194873893948 4.69694869163569E-4 0.0 1.1867735128332172E-4 2.741279663445741E-4 56-57 3.3977994277210968 4.763809171231928E-4 1.6715119899059396E-6 1.2034886327322766E-4 2.77470990324386E-4 58-59 3.9316385694973066 4.964390610020641E-4 3.3430239798118792E-6 1.2034886327322766E-4 2.908430862436335E-4 60-61 4.495588328259681 5.014535969717819E-4 3.3430239798118792E-6 1.2703491123285143E-4 3.0254367017297506E-4 62-63 5.088039037961943 5.064681329414998E-4 3.3430239798118792E-6 1.2703491123285143E-4 3.226018140518464E-4 64-65 5.709313300418142 5.164972048809354E-4 3.3430239798118792E-6 1.2703491123285143E-4 3.4767449390043543E-4 66-67 6.353977030149095 5.432413967194304E-4 3.3430239798118792E-6 1.2703491123285143E-4 3.560320538499651E-4 68-69 7.026663658390822 5.599565166184899E-4 3.3430239798118792E-6 1.303779352126633E-4 3.710756617591186E-4 70-71 7.719717660229552 5.699855885579254E-4 3.3430239798118792E-6 1.303779352126633E-4 3.7776170971874233E-4 72-73 8.43467515518401 5.833576844771729E-4 3.3430239798118792E-6 1.3372095919247516E-4 3.8277624568846017E-4 74-75 9.168585924592008 6.10101876315668E-4 3.3430239798118792E-6 1.4040700715209893E-4 3.8946229364808396E-4 76-77 9.919979037902436 6.234739722349154E-4 3.3430239798118792E-6 1.4040700715209893E-4 4.045059015572374E-4 78-79 10.692135673151476 6.335030441743511E-4 3.3430239798118792E-6 1.4207851914200487E-4 4.1954950946639086E-4 80-81 11.479198852326496 6.61918748002752E-4 3.3430239798118792E-6 1.4375003113191082E-4 4.212210214562968E-4 82-83 12.28040135142413 6.903344518311531E-4 3.3430239798118792E-6 1.470930551117227E-4 4.262355574260146E-4 84-85 13.089670567385049 7.154071316797421E-4 3.3430239798118792E-6 1.5377910307134643E-4 4.295785814058265E-4 86-87 13.91319610910788 7.47165859487955E-4 3.3430239798118792E-6 1.6046515103097022E-4 4.3125009339573244E-4 88 14.5360232062027 7.555234194374848E-4 3.3430239798118792E-6 1.6715119899059398E-4 4.3125009339573244E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 166925 0.0 85.37595 8 AGTGGTA 170005 0.0 83.956696 5 TGGTATC 170385 0.0 83.50163 7 GTGGTAT 171175 0.0 83.3742 6 GTATCAA 170815 0.0 83.31618 9 AAGCAGT 178135 0.0 80.44679 1 GCAGTGG 178650 0.0 80.352585 3 CAGTGGT 179435 0.0 79.38477 4 AGCAGTG 187460 0.0 76.633575 2 CAACGCA 163850 0.0 44.565548 12-13 ATCAACG 165730 0.0 44.191795 10-11 ACGCAGA 166065 0.0 43.923214 14-15 TCAACGC 163675 0.0 43.656136 12-13 AACGCAG 165040 0.0 43.348984 14-15 AGAGTAC 167065 0.0 43.17906 18-19 GAGTACT 162700 0.0 42.8779 20-21 CGCAGAG 168065 0.0 42.464962 16-17 TATCAAC 171145 0.0 41.591484 10-11 AGTACTT 172625 0.0 41.360718 20-21 GTACTTT 172850 0.0 40.38851 22-23 >>END_MODULE