FastQCFastQC Report
Thu 26 May 2016
SRR1660754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29913037
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14299804.780457430651391No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA696630.23288507950563497No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1791300.085.499168
AGTGGTA1827600.084.16345
GTGGTAT1834200.083.786386
GTATCAA1826100.083.759149
TGGTATC1834500.083.562627
GCAGTGG1900800.081.038463
AAGCAGT1865400.080.802971
CAGTGGT1918300.080.0517354
AGCAGTG1989950.077.318192
CAACGCA1754700.044.66430712-13
ATCAACG1773550.044.2823310-11
ACGCAGA1785950.044.00841514-15
TCAACGC1751650.043.70115312-13
AACGCAG1768900.043.32188414-15
AGAGTAC1807600.043.2538618-19
GAGTACT1772300.042.774820-21
CGCAGAG1805700.042.58606316-17
TATCAAC1833100.041.73887610-11
AGTACTT1867400.041.6158420-21
GTACTTT1854650.040.8976122-23
Produced by FastQC (version 0.11.5)