##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660754_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29913037 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.004006480518846 33.0 31.0 34.0 27.0 34.0 2 31.789612602692266 34.0 31.0 34.0 28.0 34.0 3 32.28960315864952 34.0 31.0 34.0 30.0 34.0 4 35.824697204767276 37.0 35.0 37.0 35.0 37.0 5 35.88358580909053 37.0 35.0 37.0 35.0 37.0 6 35.917181093982535 37.0 35.0 37.0 35.0 37.0 7 35.91918811185905 37.0 35.0 37.0 35.0 37.0 8 35.920507603423886 37.0 35.0 37.0 35.0 37.0 9 37.63279813413797 39.0 37.0 39.0 35.0 39.0 10-11 37.57982078182165 39.0 37.0 39.0 35.0 39.0 12-13 37.63127501898253 39.0 37.0 39.0 35.0 39.0 14-15 38.95571347369376 40.0 38.0 41.0 35.0 41.0 16-17 38.97070345949828 40.0 38.0 41.0 35.0 41.0 18-19 38.95548382466147 40.0 38.0 41.0 35.0 41.0 20-21 38.896521172357055 40.0 38.0 41.0 35.0 41.0 22-23 38.79936846599695 40.0 38.0 41.0 34.5 41.0 24-25 38.73964637893505 40.0 38.0 41.0 34.0 41.0 26-27 38.691168970907235 40.0 38.0 41.0 34.0 41.0 28-29 38.5857475788901 40.0 38.0 41.0 34.0 41.0 30-31 38.488073394219384 40.0 38.0 41.0 34.0 41.0 32-33 38.38243796509194 40.0 38.0 41.0 34.0 41.0 34-35 38.272971647780196 40.0 38.0 41.0 34.0 41.0 36-37 38.093558103110695 40.0 38.0 41.0 33.0 41.0 38-39 37.707514435929724 40.0 36.0 41.0 33.0 41.0 40-41 37.559366355880215 40.0 36.0 41.0 33.0 41.0 42-43 37.40251763804524 40.0 36.0 41.0 32.5 41.0 44-45 37.287360257000984 39.5 35.0 41.0 32.0 41.0 46-47 37.15597729177415 39.0 35.0 41.0 31.5 41.0 48-49 37.28145094394795 39.5 35.0 41.0 32.0 41.0 50-51 37.19483730455052 39.5 35.0 41.0 31.5 41.0 52-53 36.953702795205984 39.0 35.0 41.0 31.0 41.0 54-55 36.503636992793474 39.0 35.0 41.0 29.5 41.0 56-57 36.251857476056344 39.0 35.0 41.0 28.5 41.0 58-59 36.06598866908766 39.0 35.0 41.0 28.0 41.0 60-61 35.8682204184082 39.0 35.0 41.0 28.0 41.0 62-63 35.57993335815418 38.5 35.0 40.5 27.5 41.0 64-65 35.33332160489087 38.0 35.0 40.0 27.0 41.0 66-67 35.067016899688255 37.0 34.0 40.0 27.0 41.0 68-69 34.77397241543879 37.0 34.0 39.5 26.0 41.0 70-71 34.44278920926685 36.0 34.0 39.0 26.0 41.0 72-73 34.12913553378081 36.0 34.0 39.0 26.0 41.0 74-75 33.77715973807675 35.0 34.0 38.5 26.0 40.0 76-77 31.6895584189596 33.5 30.5 35.5 22.5 38.5 78-79 32.96211554848142 35.0 33.0 37.0 25.5 39.0 80-81 32.9534315756705 35.0 33.0 36.0 25.5 39.0 82-83 32.740215762779286 35.0 33.0 36.0 25.0 37.5 84-85 32.51101327491421 35.0 33.0 36.0 25.0 37.0 86-87 32.309747117953954 35.0 33.0 35.0 25.0 37.0 88-89 32.096700177919075 35.0 33.0 35.0 24.5 36.0 90-91 31.942472758616923 35.0 33.0 35.0 24.0 36.0 92-93 31.81784189950355 35.0 33.0 35.0 24.0 36.0 94-95 31.72235416952147 35.0 33.0 35.0 23.5 35.5 96-97 31.6224002765082 35.0 33.0 35.0 23.0 35.0 98-99 31.510779380241466 35.0 33.0 35.0 22.5 35.0 100 31.32586005894353 35.0 32.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.741549137769681 1101 2 1.1918791081694984 1101 3 0.7550200055307954 1101 4 0.5954382743166065 1101 5 0.46915070758686284 1101 6 0.40845986066059936 1101 7 0.3681559017696756 1101 8 0.41466338020082105 1101 9 0.48357104613405966 1101 10-11 0.49344846589099944 1101 12-13 0.45151840563408996 1101 14-15 0.6079922721716784 1101 16-17 0.5771448606547054 1101 18-19 0.5272680427245078 1101 20-21 0.5554862733824351 1101 22-23 0.5346512841416313 1101 24-25 0.5854943390645246 1101 26-27 0.5525978828477847 1101 28-29 0.5784288706179126 1101 30-31 0.5605970820245858 1101 32-33 0.5495778011957171 1101 34-35 0.5546109514006972 1101 36-37 0.5405431710871227 1101 38-39 0.6302047478490991 1101 40-41 0.6084782341213852 1101 42-43 0.5579060402525755 1101 44-45 0.540311882481582 1101 46-47 0.5071516317481439 1101 48-49 0.5028254152300988 1101 50-51 0.48994514204591866 1101 52-53 0.48822118233554335 1101 54-55 0.518715536722361 1101 56-57 0.5461784852443756 1101 58-59 0.5634267251341427 1101 60-61 0.4936819157662313 1101 62-63 0.5697056921756527 1101 64-65 0.5180715523064592 1101 66-67 0.44597325197769067 1101 68-69 0.45885517193420355 1101 70-71 0.48693562850571936 1101 72-73 0.440216922523895 1101 74-75 0.4355647480403704 1101 76-77 0.3683373191543886 1101 78-79 0.5158405193170097 1101 80-81 0.4695795199115409 1101 82-83 0.4465801301039036 1101 84-85 0.4126387316097535 1101 86-87 0.3643518834417918 1101 88-89 0.3787438569057642 1101 90-91 0.4134191772037461 1101 92-93 0.3416177453279339 1101 94-95 0.3017685679277946 1101 96-97 0.2813652506530637 1101 98-99 0.2728811503525712 1101 100 0.2530434122153231 1102 1 1.6720897880639924 1102 2 1.1332815659056727 1102 3 0.7123508411760611 1102 4 0.5653723006548432 1102 5 0.44303234473589725 1102 6 0.412942360693485 1102 7 0.37247366912306035 1102 8 0.37751890826073264 1102 9 0.4729262002865724 1102 10-11 0.4598098489772795 1102 12-13 0.3912699968864217 1102 14-15 0.5648609258942869 1102 16-17 0.541019120317138 1102 18-19 0.5270439081443712 1102 20-21 0.5653801957858278 1102 22-23 0.5433232715484522 1102 24-25 0.5771732053581644 1102 26-27 0.5978940645296618 1102 28-29 0.5804152347349572 1102 30-31 0.5638237326617315 1102 32-33 0.5366606013498014 1102 34-35 0.5460949149360133 1102 36-37 0.5515574703286674 1102 38-39 0.6338205073851526 1102 40-41 0.6190552707629067 1102 42-43 0.6431104833867423 1102 44-45 0.5952734741796206 1102 46-47 0.5952306614456688 1102 48-49 0.5759985124643592 1102 50-51 0.5570901991884512 1102 52-53 0.5219969153893231 1102 54-55 0.5659930690504993 1102 56-57 0.6603803470310368 1102 58-59 0.61438536627292 1102 60-61 0.5781951813119477 1102 62-63 0.6239534074316495 1102 64-65 0.6353344569247383 1102 66-67 0.5888222751953123 1102 68-69 0.5452738916236868 1102 70-71 0.5592207950023393 1102 72-73 0.5175966569900226 1102 74-75 0.49810521853764556 1102 76-77 0.3805495614422405 1102 78-79 0.5232808756791343 1102 80-81 0.49887027123174477 1102 82-83 0.5164958798050279 1102 84-85 0.49964792626291654 1102 86-87 0.48349810717521535 1102 88-89 0.522362192098818 1102 90-91 0.49592902020556195 1102 92-93 0.49294710770506 1102 94-95 0.42635836729418486 1102 96-97 0.39156885534247365 1102 98-99 0.45842800374483517 1102 100 0.3617457207799468 1103 1 1.6385290898632263 1103 2 1.0998995219582746 1103 3 0.6420667454956259 1103 4 0.5222149575272184 1103 5 0.39853921926020064 1103 6 0.35272426639618715 1103 7 0.32455661727495055 1103 8 0.33916385264698334 1103 9 0.41432823091835047 1103 10-11 0.38000336871395035 1103 12-13 0.3676768792962406 1103 14-15 0.525420732696908 1103 16-17 0.4731219522870376 1103 18-19 0.40171650979833373 1103 20-21 0.4208938597688743 1103 22-23 0.43460001374325685 1103 24-25 0.4733093305663374 1103 26-27 0.4299344069773454 1103 28-29 0.4641525265489008 1103 30-31 0.46565713640691087 1103 32-33 0.45428821404454567 1103 34-35 0.4672857603964573 1103 36-37 0.4828561945668355 1103 38-39 0.5552763548143673 1103 40-41 0.49746999050903185 1103 42-43 0.4609373469212912 1103 44-45 0.4651261408801233 1103 46-47 0.4736435735050506 1103 48-49 0.44038233374816826 1103 50-51 0.43092960244144507 1103 52-53 0.40171581772872145 1103 54-55 0.47334273113639114 1103 56-57 0.5732059544073422 1103 58-59 0.5623169250116362 1103 60-61 0.5668898532096733 1103 62-63 0.5761841339747775 1103 64-65 0.5160236944253285 1103 66-67 0.5244976523805036 1103 68-69 0.5234642328663099 1103 70-71 0.5032906972507831 1103 72-73 0.4645872063996421 1103 74-75 0.42630131022811213 1103 76-77 0.27925482462566364 1103 78-79 0.413283454332003 1103 80-81 0.4591081277985225 1103 82-83 0.43433871969136817 1103 84-85 0.4170578844762787 1103 86-87 0.4036560252985453 1103 88-89 0.4330503121463707 1103 90-91 0.4327141227859208 1103 92-93 0.41076106141161617 1103 94-95 0.3546199119679869 1103 96-97 0.3275124265767424 1103 98-99 0.36943773253084444 1103 100 0.2949358722359179 1104 1 1.5638633255580459 1104 2 1.0582622569608127 1104 3 0.6087721278143974 1104 4 0.5123580283053997 1104 5 0.3836203248136556 1104 6 0.345846576476454 1104 7 0.29344729420185445 1104 8 0.31891998060859095 1104 9 0.3845805982173758 1104 10-11 0.37474429701556744 1104 12-13 0.34336433685469814 1104 14-15 0.4926533692107782 1104 16-17 0.4650269320991569 1104 18-19 0.388911687721361 1104 20-21 0.40495329039243444 1104 22-23 0.39652706470354104 1104 24-25 0.442757102542231 1104 26-27 0.43163305355479764 1104 28-29 0.42288972175885675 1104 30-31 0.42051535504423754 1104 32-33 0.4153673002735516 1104 34-35 0.40180479654967627 1104 36-37 0.44221787581057725 1104 38-39 0.49855128000503157 1104 40-41 0.4654034177929063 1104 42-43 0.4291648748260215 1104 44-45 0.43556308363403673 1104 46-47 0.4589149584686041 1104 48-49 0.4487836620333425 1104 50-51 0.43019735694995376 1104 52-53 0.4221415660460721 1104 54-55 0.4453057634893227 1104 56-57 0.47504379632806604 1104 58-59 0.44112811690290954 1104 60-61 0.4434517884160769 1104 62-63 0.4730860980007705 1104 64-65 0.4731783563145058 1104 66-67 0.4487372572325228 1104 68-69 0.4383086078896383 1104 70-71 0.4435711516633134 1104 72-73 0.40230460522415257 1104 74-75 0.3769367453188366 1104 76-77 0.3676423440728307 1104 78-79 0.4253488117590507 1104 80-81 0.39524010768905526 1104 82-83 0.37256585785893037 1104 84-85 0.3604877089221574 1104 86-87 0.3704270745379219 1104 88-89 0.4083674322596025 1104 90-91 0.4050448971979641 1104 92-93 0.3965248233475158 1104 94-95 0.3881314766356496 1104 96-97 0.3470713351516217 1104 98-99 0.3953712271832863 1104 100 0.3236665492403148 1105 1 1.5606350542395404 1105 2 1.02475296884813 1105 3 0.6010909351409879 1105 4 0.493040177212535 1105 5 0.32699997728975205 1105 6 0.27365518875397044 1105 7 0.20962261512224245 1105 8 0.26856555399811555 1105 9 0.3137113148857935 1105 10-11 0.2948254192132893 1105 12-13 0.264318328335321 1105 14-15 0.3670696806923033 1105 16-17 0.3102310426864605 1105 18-19 0.3117440076751379 1105 20-21 0.3031282865560101 1105 22-23 0.28888669086317265 1105 24-25 0.3321755710158598 1105 26-27 0.28387061894992627 1105 28-29 0.3168361637778787 1105 30-31 0.3490938716950964 1105 32-33 0.34664229422811843 1105 34-35 0.3218721240930975 1105 36-37 0.38443033631237 1105 38-39 0.42167872039604504 1105 40-41 0.42045057366243554 1105 42-43 0.397860377691579 1105 44-45 0.3970304535624365 1105 46-47 0.39242740127074427 1105 48-49 0.3643317966255992 1105 50-51 0.31091110150269685 1105 52-53 0.29756054101402896 1105 54-55 0.39837395153697486 1105 56-57 0.3915088858413327 1105 58-59 0.4053134200239654 1105 60-61 0.37891280324731724 1105 62-63 0.40206367681781074 1105 64-65 0.3689151879068504 1105 66-67 0.3978923720585641 1105 68-69 0.4020561072649187 1105 70-71 0.3940950449999363 1105 72-73 0.3289679755156456 1105 74-75 0.33370128204623484 1105 76-77 0.27767993620370035 1105 78-79 0.39342713964720133 1105 80-81 0.36676663679465804 1105 82-83 0.343977866718717 1105 84-85 0.378893789842941 1105 86-87 0.3812017889592383 1105 88-89 0.3867008312053315 1105 90-91 0.36269536419991866 1105 92-93 0.35551382578890056 1105 94-95 0.3433058160675486 1105 96-97 0.3499412455114168 1105 98-99 0.34575250967928284 1105 100 0.2944827072518308 1106 1 1.4491906474270309 1106 2 0.8966678728632296 1106 3 0.48199988515305137 1106 4 0.42534946631347736 1106 5 0.261165575733564 1106 6 0.23644195737003315 1106 7 0.17752470063407344 1106 8 0.20179877865098206 1106 9 0.2600682548324471 1106 10-11 0.2398968981861671 1106 12-13 0.19733574788361352 1106 14-15 0.3184925869916526 1106 16-17 0.26383248227202216 1106 18-19 0.2150795578603848 1106 20-21 0.21955418929613302 1106 22-23 0.2167092930035821 1106 24-25 0.2555092697841843 1106 26-27 0.22662866160667505 1106 28-29 0.2792857242207205 1106 30-31 0.25399079140047576 1106 32-33 0.24098312622145812 1106 34-35 0.28168943520785916 1106 36-37 0.2537327388766286 1106 38-39 0.32361659357310657 1106 40-41 0.30275122419804035 1106 42-43 0.23256368834690733 1106 44-45 0.24197485949403585 1106 46-47 0.2890625583731534 1106 48-49 0.31740391627852205 1106 50-51 0.2716870297647489 1106 52-53 0.21399221819176972 1106 54-55 0.26582182710183133 1106 56-57 0.2580170167840379 1106 58-59 0.20613200658726072 1106 60-61 0.22709662871595526 1106 62-63 0.2793186306477864 1106 64-65 0.2631472847365046 1106 66-67 0.25744502775860667 1106 68-69 0.24290925281879083 1106 70-71 0.2699820216255375 1106 72-73 0.22715905075379794 1106 74-75 0.1771974903300233 1106 76-77 0.10233079912365639 1106 78-79 0.24683206862079032 1106 80-81 0.25724943840626935 1106 82-83 0.24720082785962916 1106 84-85 0.26122604852769626 1106 86-87 0.21692159435583136 1106 88-89 0.2592192019738562 1106 90-91 0.28128667049950806 1106 92-93 0.23795308503025225 1106 94-95 0.24849271822737506 1106 96-97 0.2709546644529546 1106 98-99 0.2859544048003073 1106 100 0.2288748759105772 1107 1 1.5616000968474282 1107 2 1.0274828743311808 1107 3 0.6015356259702926 1107 4 0.49474677514504606 1107 5 0.33904580300745124 1107 6 0.30522658945488956 1107 7 0.2588052451170171 1107 8 0.26912768654757 1107 9 0.33594470855393865 1107 10-11 0.29674667599424254 1107 12-13 0.29621221917378904 1107 14-15 0.4252485183825385 1107 16-17 0.3782725042985362 1107 18-19 0.323227656970964 1107 20-21 0.3180580656739309 1107 22-23 0.3487220360419272 1107 24-25 0.415447596893884 1107 26-27 0.34975869007420357 1107 28-29 0.3249735457681382 1107 30-31 0.3574687404200816 1107 32-33 0.3721049605060429 1107 34-35 0.3824589201982249 1107 36-37 0.4286026122564124 1107 38-39 0.45463253268940207 1107 40-41 0.42251697285256995 1107 42-43 0.41489636962614185 1107 44-45 0.4160755437519299 1107 46-47 0.46337314462721935 1107 48-49 0.44430276631771903 1107 50-51 0.41789431661395327 1107 52-53 0.383471443629233 1107 54-55 0.4423901068885314 1107 56-57 0.3925636244221806 1107 58-59 0.36999130879588904 1107 60-61 0.34020725054669754 1107 62-63 0.3747630811168179 1107 64-65 0.3659081759312386 1107 66-67 0.34818052687101897 1107 68-69 0.3559620498495164 1107 70-71 0.35733299951067465 1107 72-73 0.3730186311881667 1107 74-75 0.365202083726345 1107 76-77 0.2822330068554031 1107 78-79 0.4032565325838533 1107 80-81 0.388593561402665 1107 82-83 0.3800905440982021 1107 84-85 0.3841417859604661 1107 86-87 0.3689707582002413 1107 88-89 0.4128849082535595 1107 90-91 0.40412380398243997 1107 92-93 0.4072266380122862 1107 94-95 0.41084589835035956 1107 96-97 0.353592847653335 1107 98-99 0.38110420751749174 1107 100 0.2834445894756996 1108 1 1.3928262722063067 1108 2 0.8702985699290302 1108 3 0.44225729747672915 1108 4 0.3668416394720282 1108 5 0.20916351513653098 1108 6 0.16339973678630315 1108 7 0.11648180050421075 1108 8 0.12451953241121316 1108 9 0.1522834289021091 1108 10-11 0.11910169586318631 1108 12-13 0.10877189576896029 1108 14-15 0.17923784302116275 1108 16-17 0.12085520215855894 1108 18-19 0.11158642192344104 1108 20-21 0.13413676696861643 1108 22-23 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sequence quality scores pass #Quality Count 6 55.0 7 829.0 8 1045.0 9 422.0 10 200.0 11 189.0 12 285.0 13 1172.0 14 5311.0 15 14111.0 16 25347.0 17 34029.0 18 42772.0 19 54184.0 20 72708.0 21 104806.0 22 159001.0 23 249988.0 24 391224.0 25 483651.0 26 474677.0 27 421966.0 28 411692.0 29 455886.0 30 539366.0 31 654868.0 32 808874.0 33 1022224.0 34 1373451.0 35 2020710.0 36 3405365.0 37 6372287.0 38 8062963.0 39 2242717.0 40 4662.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.847389417378132 34.19343015334966 26.980756487129288 20.978423942142925 2 21.48079782069604 31.77514205595373 28.061931658761363 18.682128464588867 3 24.512007924838926 26.787911906103012 27.012185355836653 21.687894813221405 4 20.02837425033105 27.392678316146903 26.82586525734582 25.75308217617623 5 21.014812939076773 32.10744280496668 26.409427124564388 20.46831713139216 6 26.112621062181013 26.50969876445511 26.797496355853134 20.58018381751074 7 20.708047798690586 27.00407183663765 31.861733063078816 20.42614730159295 8 25.438888067433606 26.458259654477743 27.126229275883958 20.976623002204693 9 25.618695955211766 26.11342338793617 27.357760430677768 20.910120226174293 10-11 20.380366259701415 29.494434149230653 29.478711907453597 20.64648768361434 12-13 20.469578197626674 26.81927950010559 29.232917740849917 23.478224561417818 14-15 20.461762207561872 32.355808606127155 26.399729990639198 20.78269919567177 16-17 23.35427191829435 26.733860222885426 26.382538489823016 23.529329368997203 18-19 20.610840100328897 29.51793745040725 26.33695769198001 23.53426475728384 20-21 23.396785062887197 29.508275592269467 26.32658842548149 20.768350919361843 22-23 23.382304845877066 26.80007884187754 29.099126578153868 20.718489734091527 24-25 20.73270835814855 29.481633273504908 26.18973888769412 23.595919480652423 26-27 20.757381821945646 26.72215766515093 31.73324904473254 20.78721146817089 28-29 20.765203479673428 26.802367141791724 31.689408534479462 20.743020844055387 30-31 20.785020925825755 26.78180754431588 31.65453243681008 20.778639093048294 32-33 20.797357379571903 26.816895148991225 31.59449111083208 20.791256360604788 34-35 20.85598831038119 26.835543311767374 31.53833728083176 20.77013109701967 36-37 20.857993499188673 26.819840801512772 31.489517337683765 20.83264836161479 38-39 20.905847841528093 26.808513291378606 31.49276684945096 20.792872017642342 40-41 20.92326004195208 26.88104424379521 31.458126168704386 20.73756954554832 42-43 20.94178301576749 26.9302224442998 31.359203059729886 20.768791480202825 44-45 20.99915510865369 26.958827984458438 31.301749051639177 20.740267855248693 46-47 21.04035734355563 27.05130988114413 31.199547386404213 20.708785388896022 48-49 21.083021737609698 27.0618017198964 31.086454242503336 20.768722299990564 50-51 21.078224186999133 27.14431336410275 30.99095889193732 20.7865035569608 52-53 21.180199901586686 27.285016400603375 30.753024333977574 20.781759363832368 54-55 21.506927670112823 27.49759761762564 30.050041762757786 20.945432949503754 56-57 21.82668468995436 27.94100241062504 29.03364068634005 21.198672213080552 58-59 21.93192320409304 28.41151663473437 28.4014842162494 21.255075944923192 60-61 21.979495595192027 28.65488624596343 28.01333610203491 21.35228205680963 62-63 22.007742498321253 28.795763053094603 27.804839538668773 21.391654909915367 64-65 21.952089188190754 28.912869491923725 27.74023290227297 21.394808417612552 66-67 21.93286266184991 28.787433531059932 27.79176401515231 21.48793979193785 68-69 21.904208010288816 28.637451044686742 27.93454615925893 21.523794785765517 70-71 21.832363930137713 28.59048198125585 28.02460583401693 21.552548254589507 72-73 21.816574623388007 28.520129920286735 28.02767270749453 21.635622748830723 74-75 21.837142242493908 28.519947065687656 27.950284738393204 21.692625953425228 76-77 21.867207790964983 28.552341337830395 27.810067831818756 21.77038303938587 78-79 21.945468723246776 28.622176278099104 27.5251773165056 21.90717768214852 80-81 21.992042864997863 28.72208736821367 27.27647754457601 22.009392222212455 82-83 21.990220923546012 28.831171349479117 27.055204140310522 22.12340358666435 84-85 22.019209304257583 28.83564142576334 26.893998803670492 22.25115046630858 86-87 22.043713472467434 28.792674439794574 26.790990979365375 22.372621108372613 88-89 22.115562882384804 28.770724450921726 26.689890828331126 22.423821838362343 90-91 22.13783730924883 28.64342966800228 26.62827321401408 22.59045980873481 92-93 22.164249111036398 28.554617003333167 26.656591681763626 22.624542203866813 94-95 22.187426744064606 28.520196339613612 26.65616751289739 22.636209403424388 96-97 22.209478469889845 28.36769298811232 26.670622016249396 22.752206525748438 98-99 22.175469175226603 28.256793265648874 26.737848870269914 22.82988868885461 100 22.182650882809003 28.219234065063148 26.721618717661933 22.87649633446592 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 48474.0 1 30918.0 2 10427.0 3 7111.5 4 6667.0 5 6711.5 6 6969.5 7 7183.0 8 7490.0 9 7920.0 10 8634.0 11 11401.5 12 103146.5 13 125189.0 14 55438.0 15 57045.5 16 65373.0 17 73956.5 18 82105.0 19 91013.5 20 102786.5 21 120191.0 22 144732.0 23 172645.5 24 199477.5 25 230516.5 26 267462.0 27 310025.0 28 362356.5 29 415680.5 30 463210.5 31 511328.0 32 564614.5 33 615380.0 34 655878.0 35 705315.5 36 769358.5 37 824361.0 38 873849.5 39 913775.5 40 936357.0 41 964707.0 42 999640.5 43 1009399.0 44 1004059.0 45 1016620.5 46 1027916.5 47 1026241.0 48 1022188.0 49 1014257.5 50 1004075.0 51 995784.5 52 981046.0 53 939228.5 54 877529.5 55 813982.5 56 742444.0 57 663410.0 58 583480.0 59 497473.0 60 407382.5 61 329033.5 62 260425.0 63 196193.5 64 145463.5 65 106988.0 66 77661.5 67 58090.0 68 44246.0 69 33677.5 70 26126.5 71 21514.0 72 18382.5 73 14665.5 74 11339.5 75 9294.5 76 7209.0 77 4665.5 78 2854.0 79 1929.0 80 1330.0 81 929.0 82 635.0 83 431.5 84 290.5 85 190.0 86 122.0 87 72.0 88 51.0 89 31.0 90 19.0 91 19.0 92 18.0 93 10.5 94 7.5 95 8.5 96 6.5 97 3.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10821368622651052 2 0.0 3 0.0 4 0.0 5 0.009477472982766678 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.853199158614352E-5 20-21 0.01074447907111538 22-23 0.0 24-25 9.226746184280787E-4 26-27 0.005078053425334244 28-29 0.0 30-31 0.0 32-33 3.3430239798118797E-6 34-35 0.0 36-37 5.014535969717819E-6 38-39 0.0 40-41 0.007501745810697857 42-43 0.03869215954234269 44-45 0.014901529390011452 46-47 0.004780524291130988 48-49 0.001602979998319796 50-51 0.0 52-53 0.0013973840235613655 54-55 7.354652755586135E-5 56-57 8.541426268419352E-4 58-59 3.510175178802474E-5 60-61 3.292878620114701E-4 62-63 2.7579947833448E-4 64-65 6.184594362651977E-5 66-67 3.3263088599128196E-4 68-69 5.699855885579254E-4 70-71 4.379361413553562E-4 72-73 0.0012954217921771032 74-75 0.0016447677980674447 76-77 0.00249891042490938 78-79 0.004481323644937824 80-81 0.0042322683584418395 82-83 0.007478344642839174 84-85 0.0023718755136765282 86-87 0.0028081401430419786 88-89 0.005547748294497813 90-91 0.005639681453942641 92-93 0.0055510913184776255 94-95 0.0026242738241523253 96-97 0.0055093035187299775 98-99 0.007790917384951585 100 0.3002871289866021 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.9913037E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.42013436246392 #Duplication Level Percentage of deduplicated Percentage of total 1 65.94497830119626 26.655048834641203 2 17.756331933858398 14.354266451021305 3 6.54272310760865 7.933732413178173 4 2.9732666836803068 4.80719355399182 5 1.5895529902371204 3.212497272082035 6 1.0860467033640357 2.633889220433119 7 0.7437537769678654 2.1043839318341795 8 0.5572977864650963 1.8020841127058336 9 0.4114649070703914 1.4968320146301557 >10 2.0814582931605385 14.957264453219207 >50 0.16667620216819332 4.832344123978265 >100 0.14528095205582267 9.499025384803486 >500 6.409875555431059E-4 0.15715547276086428 >1k 4.8630833963580393E-4 0.3859916327514436 >5k 8.213254455981624E-6 0.01912873650001292 >10k+ 3.285301780309838E-5 5.149162391468799 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1429980 4.780457430651391 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69663 0.23288507950563497 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.007568606290294095 6.6860479596237585E-6 0.0 0.0 2.0058143878871277E-5 2 0.007568606290294095 3.343023979811879E-5 0.0 0.0 2.3401167858683156E-5 3 0.007575292338253718 4.0116287757742554E-5 0.0 0.0 2.6744191838495034E-5 4 0.007578635362233531 4.345931173755443E-5 0.0 3.3430239798118792E-6 2.6744191838495034E-5 5 0.007578635362233531 5.014535969717819E-5 0.0 3.3430239798118792E-6 3.677326377793067E-5 6 0.007578635362233531 5.014535969717819E-5 0.0 3.3430239798118792E-6 3.677326377793067E-5 7 0.007578635362233531 5.683140765680195E-5 0.0 3.3430239798118792E-6 4.680233571736631E-5 8 0.007578635362233531 6.686047959623758E-5 0.0 3.3430239798118792E-6 4.680233571736631E-5 9 0.007581978386213342 8.023257551548511E-5 0.0 3.3430239798118792E-6 4.680233571736631E-5 10-11 0.007588664434172967 9.193315944482669E-5 0.0 3.3430239798118792E-6 4.680233571736631E-5 12-13 0.007600365018102307 9.861920740445045E-5 0.0 3.3430239798118792E-6 6.017443163661383E-5 14-15 0.0076354667698903325 1.1031979133379202E-4 0.0 1.0029071939435639E-5 7.856106352557916E-5 16-17 0.007658867937749015 1.2369188725303953E-4 0.0 1.0029071939435639E-5 9.69476954145445E-5 18-19 0.007722385393365441 1.5043607909153456E-4 0.0 1.3372095919247517E-5 1.0697676735398014E-4 20-21 0.007792588896941491 1.688227109804999E-4 0.0 1.8386631888965336E-5 1.136628153136039E-4 22-23 0.007958068583942178 1.8720934286946524E-4 0.0 3.0087215818306916E-5 1.2369188725303953E-4 24-25 0.008730307123278724 1.93895390829089E-4 0.0 3.0087215818306916E-5 1.3706398317228706E-4 26-27 0.011307778611713682 2.055959747584306E-4 0.0 3.0087215818306916E-5 1.5879363904106427E-4 28-29 0.019219044859938493 2.49055286495985E-4 0.0 3.343023979811879E-5 1.771802709300296E-4 30-31 0.038259237936957055 2.691134303748563E-4 0.0 3.677326377793067E-5 2.055959747584306E-4 32-33 0.07681099047214765 2.8415703828400975E-4 0.0 4.0116287757742554E-5 2.1562504669786622E-4 34-35 0.14174588825601359 3.0922971813259885E-4 0.0 4.178779974764849E-5 2.3568319057673748E-4 36-37 0.2375251967896138 3.242733260417523E-4 0.0 4.345931173755443E-5 2.440407505262672E-4 38-39 0.370891795440229 3.2761635002156417E-4 0.0 4.680233571736631E-5 2.5406982246570285E-4 40-41 0.543869885227635 3.343023979811879E-4 0.0 5.014535969717819E-5 2.557413344556088E-4 42-43 0.7568756726373187 3.526890298701533E-4 0.0 5.014535969717819E-5 2.5908435843542064E-4 44-45 1.0129713676347873 3.6773263777930675E-4 0.0 5.014535969717819E-5 2.6409889440513843E-4 46-47 1.3117340776865953 3.794332217086483E-4 0.0 5.1816871687084134E-5 2.808140143041979E-4 48-49 1.6548754310704057 4.045059015572374E-4 0.0 5.348838367699007E-5 2.858285502739157E-4 50-51 2.0394034213242875 4.262355574260146E-4 0.0 5.348838367699007E-5 2.908430862436335E-4 52-53 2.464024298168053 4.329216053856384E-4 0.0 5.683140765680195E-5 2.9585762221335133E-4 54-55 2.9270247618120484 4.546512612544156E-4 0.0 5.683140765680195E-5 2.992006461931632E-4 56-57 3.427796381891949 4.8640998906262844E-4 0.0 6.017443163661383E-5 3.0588669415278696E-4 58-59 3.966683489877674 5.215117408506532E-4 0.0 6.184594362651977E-5 3.1090123012250475E-4 60-61 4.535483976434756 5.482559326891482E-4 0.0 6.686047959623758E-5 3.2427332604175233E-4 62-63 5.133388829760081 5.599565166184899E-4 0.0 7.354652755586135E-5 3.292878620114701E-4 64-65 5.760110549791383 5.750001245276433E-4 0.0 7.354652755586135E-5 3.3931693395090575E-4 66-67 6.410345428984693 5.950582684065146E-4 0.0 7.354652755586135E-5 3.4600298191052954E-4 68-69 7.088937509086757 6.184594362651977E-4 0.0 7.354652755586135E-5 3.6438961379949486E-4 70-71 7.789088750834628 6.318315321844452E-4 0.0 7.354652755586135E-5 3.7274717374902454E-4 72-73 8.512559256353676 6.619187480027521E-4 0.0 7.354652755586135E-5 3.87790781658178E-4 74-75 9.25582882139316 6.736193319320937E-4 0.0 7.521803954576729E-5 3.9113380563798986E-4 76-77 10.017481675297631 6.803053798917174E-4 0.0 7.688955153567323E-5 3.961483416077077E-4 78-79 10.799618908638397 6.853199158614353E-4 0.0 7.856106352557916E-5 3.994913655875196E-4 80-81 11.596502220754115 7.120641076999302E-4 0.0 8.023257551548511E-5 4.0116287757742554E-4 82-83 12.406969910811798 7.254362036191778E-4 0.0 8.524711148520292E-5 4.16206485486579E-4 84-85 13.228218853204373 7.321222515788016E-4 0.0 9.026164745492074E-5 4.212210214562968E-4 86-87 14.06316583635423 7.454943474980491E-4 0.0 9.193315944482669E-5 4.329216053856384E-4 88 14.694977310394796 7.521803954576729E-4 0.0 9.360467143473262E-5 4.3459311737554433E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 179130 0.0 85.49916 8 AGTGGTA 182760 0.0 84.1634 5 GTGGTAT 183420 0.0 83.78638 6 GTATCAA 182610 0.0 83.75914 9 TGGTATC 183450 0.0 83.56262 7 GCAGTGG 190080 0.0 81.03846 3 AAGCAGT 186540 0.0 80.80297 1 CAGTGGT 191830 0.0 80.051735 4 AGCAGTG 198995 0.0 77.31819 2 CAACGCA 175470 0.0 44.664307 12-13 ATCAACG 177355 0.0 44.28233 10-11 ACGCAGA 178595 0.0 44.008415 14-15 TCAACGC 175165 0.0 43.701153 12-13 AACGCAG 176890 0.0 43.321884 14-15 AGAGTAC 180760 0.0 43.25386 18-19 GAGTACT 177230 0.0 42.7748 20-21 CGCAGAG 180570 0.0 42.586063 16-17 TATCAAC 183310 0.0 41.738876 10-11 AGTACTT 186740 0.0 41.61584 20-21 GTACTTT 185465 0.0 40.89761 22-23 >>END_MODULE