FastQCFastQC Report
Thu 26 May 2016
SRR1660753_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660753_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28414852
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10967693.8598441406627773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA867590.30532976205542084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1388650.085.105188
GTGGTAT1413250.083.670316
AGTGGTA1414550.083.577555
GTATCAA1413300.083.494539
TGGTATC1413950.083.35987
GCAGTGG1510450.078.838863
AAGCAGT1511600.078.60111
CAGTGGT1519600.077.762734
AGCAGTG1581050.075.343792
CAACGCA1342750.044.7872812-13
ATCAACG1357100.044.4781110-11
TCAACGC1340600.044.22842812-13
ACGCAGA1370950.043.93770214-15
AACGCAG1361100.043.61180514-15
CGCAGAG1382750.042.9335216-17
AGAGTAC1389750.042.88920618-19
GAGTACT1354900.042.8871220-21
TATCAAC1426000.041.41169410-11
AGTACTT1460150.040.48496220-21
GTACTTT1455100.039.9646822-23
Produced by FastQC (version 0.11.5)