FastQCFastQC Report
Thu 26 May 2016
SRR1660753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28414852
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12027614.232860336559205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA659190.23198783509412613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1526750.085.556158
GTATCAA1552150.084.022869
AGTGGTA1562550.083.980365
GTGGTAT1563850.083.823956
TGGTATC1561150.083.764237
GCAGTGG1643750.080.014783
AAGCAGT1633450.078.796271
CAGTGGT1666750.078.682244
AGCAGTG1717050.076.48132
CAACGCA1481550.044.83495712-13
ATCAACG1495850.044.42829510-11
TCAACGC1477050.044.19365712-13
ACGCAGA1510100.044.11888514-15
AACGCAG1497600.043.64914314-15
CGCAGAG1525700.042.9776916-17
AGAGTAC1543800.042.9448818-19
GAGTACT1506400.042.8772320-21
TATCAAC1562550.041.74835210-11
AGTACTT1606250.040.94777720-21
GTACTTT1601000.040.3251922-23
Produced by FastQC (version 0.11.5)