##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660753_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28414852 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99015124203357 33.0 31.0 34.0 27.0 34.0 2 31.775161841420115 34.0 31.0 34.0 28.0 34.0 3 32.281111863612736 34.0 31.0 34.0 30.0 34.0 4 35.8148194120455 37.0 35.0 37.0 35.0 37.0 5 35.87307820572143 37.0 35.0 37.0 35.0 37.0 6 35.90933857406683 37.0 35.0 37.0 35.0 37.0 7 35.92004343362408 37.0 35.0 37.0 35.0 37.0 8 35.91527286504959 37.0 35.0 37.0 35.0 37.0 9 37.62475912244766 39.0 37.0 39.0 35.0 39.0 10-11 37.58193333190685 39.0 37.0 39.0 35.0 39.0 12-13 37.62186230989343 39.0 37.0 39.0 35.0 39.0 14-15 38.9441857026037 40.0 38.0 41.0 35.0 41.0 16-17 38.96023832888519 40.0 38.0 41.0 35.0 41.0 18-19 38.94954555807646 40.0 38.0 41.0 35.0 41.0 20-21 38.900167806610426 40.0 38.0 41.0 35.0 41.0 22-23 38.815627545763746 40.0 38.0 41.0 34.5 41.0 24-25 38.74767222085127 40.0 38.0 41.0 34.0 41.0 26-27 38.68836235712226 40.0 38.0 41.0 34.0 41.0 28-29 38.580175254828006 40.0 38.0 41.0 34.0 41.0 30-31 38.482989881488734 40.0 38.0 41.0 34.0 41.0 32-33 38.381214742909805 40.0 38.0 41.0 34.0 41.0 34-35 38.27563398887314 40.0 38.0 41.0 34.0 41.0 36-37 38.10598617582101 40.0 38.0 41.0 33.0 41.0 38-39 37.7557463434967 40.0 37.0 41.0 33.0 41.0 40-41 37.61530660092828 40.0 36.0 41.0 33.0 41.0 42-43 37.46747438628222 40.0 36.0 41.0 32.5 41.0 44-45 37.35999793347507 40.0 36.0 41.0 32.0 41.0 46-47 37.235940415948676 39.0 35.0 41.0 32.0 41.0 48-49 37.373514174911065 40.0 36.0 41.0 32.0 41.0 50-51 37.300225336383946 40.0 36.0 41.0 32.0 41.0 52-53 37.08220581265037 39.5 35.0 41.0 31.0 41.0 54-55 36.67399289991023 39.0 35.0 41.0 30.0 41.0 56-57 36.46042244034915 39.0 35.0 41.0 29.5 41.0 58-59 36.29578734036693 39.0 35.0 41.0 29.0 41.0 60-61 36.11152928053259 39.0 35.0 41.0 28.5 41.0 62-63 35.83044988233618 39.0 35.0 41.0 28.0 41.0 64-65 35.59358187049504 38.0 35.0 40.0 28.0 41.0 66-67 35.3318091186961 37.5 35.0 40.0 28.0 41.0 68-69 35.03796500506144 37.0 34.0 40.0 28.0 41.0 70-71 34.705616661315005 36.5 34.0 39.0 26.5 41.0 72-73 34.39395790271932 36.0 34.0 39.0 26.0 41.0 74-75 34.04618908449708 35.5 34.0 39.0 26.0 40.0 76-77 31.949697855192063 34.0 31.0 36.0 23.5 39.0 78-79 33.22880594275135 35.0 33.0 37.0 26.0 39.0 80-81 33.21703472184194 35.0 34.0 37.0 26.0 39.0 82-83 32.994724308259634 35.0 33.5 36.0 26.0 38.5 84-85 32.755439866447304 35.0 33.0 36.0 26.0 37.0 86-87 32.54385062431436 35.0 33.0 35.5 25.5 37.0 88-89 32.321329986163576 35.0 33.0 35.0 25.0 36.5 90-91 32.15926019956043 35.0 33.0 35.0 25.0 36.0 92-93 32.03317523878006 35.0 33.0 35.0 25.0 36.0 94-95 31.938422009025423 35.0 33.0 35.0 25.0 36.0 96-97 31.838473960730113 35.0 33.0 35.0 24.0 35.0 98-99 31.72925041101745 35.0 33.0 35.0 24.0 35.0 100 31.546680869567787 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7558641924997822 1101 2 1.2140407884683135 1101 3 0.7868544929535162 1101 4 0.6206187327121384 1101 5 0.47278123137889594 1101 6 0.4277779796714398 1101 7 0.37575798391171844 1101 8 0.3949886620276999 1101 9 0.5076949449410364 1101 10-11 0.5135536295255108 1101 12-13 0.4613880881611081 1101 14-15 0.6246189861685636 1101 16-17 0.61694208988078 1101 18-19 0.5606842404899126 1101 20-21 0.5875042206096524 1101 22-23 0.5674893416440554 1101 24-25 0.6030825028266591 1101 26-27 0.5895122942459992 1101 28-29 0.6256676627470767 1101 30-31 0.599169674462658 1101 32-33 0.553439343681184 1101 34-35 0.5910134408379903 1101 36-37 0.5563839355843356 1101 38-39 0.6434557686189066 1101 40-41 0.6303570940139309 1101 42-43 0.5991081387132269 1101 44-45 0.5716233251263958 1101 46-47 0.5421695141133398 1101 48-49 0.5284790544961027 1101 50-51 0.542245479817943 1101 52-53 0.538937654965828 1101 54-55 0.5337659876450616 1101 56-57 0.6001951595535289 1101 58-59 0.6035834712822137 1101 60-61 0.5618748018310313 1101 62-63 0.5903686114936235 1101 64-65 0.5657376899238926 1101 66-67 0.5086238279147679 1101 68-69 0.5241254854174855 1101 70-71 0.5170947466242453 1101 72-73 0.49750864546575 1101 74-75 0.47568597987557126 1101 76-77 0.3719746432243234 1101 78-79 0.5136101247256946 1101 80-81 0.48493357753316246 1101 82-83 0.44761345453085966 1101 84-85 0.4425278408995368 1101 86-87 0.41537023796946215 1101 88-89 0.4540998877396376 1101 90-91 0.4448683097975348 1101 92-93 0.4231371640880255 1101 94-95 0.35828675649697317 1101 96-97 0.3824078975366554 1101 98-99 0.395502925236638 1101 100 0.3245536831571947 1102 1 1.671936902687495 1102 2 1.1627956702248632 1102 3 0.7139372537920821 1102 4 0.5690797041112461 1102 5 0.4380590412380059 1102 6 0.3981258978537028 1102 7 0.3646768770964357 1102 8 0.3817688730000839 1102 9 0.4737962639526785 1102 10-11 0.4566194376565065 1102 12-13 0.40090247306101645 1102 14-15 0.5792305153896464 1102 16-17 0.5499531660524184 1102 18-19 0.5470033550631896 1102 20-21 0.5502769636582556 1102 22-23 0.5384225483170368 1102 24-25 0.5746675360082492 1102 26-27 0.5723462189081729 1102 28-29 0.5610729934145695 1102 30-31 0.5862042581629865 1102 32-33 0.547510729036297 1102 34-35 0.54697550500223 1102 36-37 0.5844863331465007 1102 38-39 0.6519314676061185 1102 40-41 0.6218694417898973 1102 42-43 0.6122118578486493 1102 44-45 0.5906823846053157 1102 46-47 0.6112436964566257 1102 48-49 0.5833109655483995 1102 50-51 0.5707696700591995 1102 52-53 0.48914563943994693 1102 54-55 0.550208470117532 1102 56-57 0.6012757737123238 1102 58-59 0.5755238106774385 1102 60-61 0.5391638003630064 1102 62-63 0.5715286051784361 1102 64-65 0.5801744246430118 1102 66-67 0.5455774730823109 1102 68-69 0.5158427963879078 1102 70-71 0.5255424580326675 1102 72-73 0.4591862881251316 1102 74-75 0.45700914543812843 1102 76-77 0.37939308053021037 1102 78-79 0.5008936827690604 1102 80-81 0.4864538184311371 1102 82-83 0.47138526325569075 1102 84-85 0.44153850915147785 1102 86-87 0.4002738076878032 1102 88-89 0.4878552183715854 1102 90-91 0.4672981812920014 1102 92-93 0.42766814872914694 1102 94-95 0.3818986026746529 1102 96-97 0.3872836547697851 1102 98-99 0.4157815434223373 1102 100 0.34090019117913783 1103 1 1.6658481809751429 1103 2 1.1235537617399238 1103 3 0.6899502498806385 1103 4 0.5488869891953101 1103 5 0.4112790876807111 1103 6 0.35968821811165697 1103 7 0.3413968427994831 1103 8 0.3729124328744575 1103 9 0.4469966450476903 1103 10-11 0.3863814455595147 1103 12-13 0.3834625665297935 1103 14-15 0.5505043802967933 1103 16-17 0.49214252867868424 1103 18-19 0.4295886144811405 1103 20-21 0.4492992647876193 1103 22-23 0.46363525442809816 1103 24-25 0.5071025444293227 1103 26-27 0.46001592658682 1103 28-29 0.5106929861653953 1103 30-31 0.517093780564764 1103 32-33 0.5174021667363249 1103 34-35 0.5240381998160544 1103 36-37 0.5389918226176746 1103 38-39 0.5770616303736489 1103 40-41 0.5422005272914774 1103 42-43 0.5468319368699568 1103 44-45 0.5414779197018191 1103 46-47 0.5497440421329856 1103 48-49 0.5151062637873736 1103 50-51 0.4635987507541586 1103 52-53 0.43878135278509234 1103 54-55 0.4780026200579286 1103 56-57 0.553945910085325 1103 58-59 0.5225980339855099 1103 60-61 0.5242414051995468 1103 62-63 0.5309502694584083 1103 64-65 0.4950789520161507 1103 66-67 0.4661544148629062 1103 68-69 0.47834026417316977 1103 70-71 0.5049301139526889 1103 72-73 0.4515205669111353 1103 74-75 0.4462910628648231 1103 76-77 0.28198097208155914 1103 78-79 0.41449797357295637 1103 80-81 0.4371427728929902 1103 82-83 0.4323716422194721 1103 84-85 0.4099469912340723 1103 86-87 0.37096524896541183 1103 88-89 0.4185519100998718 1103 90-91 0.4171947317779612 1103 92-93 0.3879298877448534 1103 94-95 0.340850823047802 1103 96-97 0.3498963222056197 1103 98-99 0.4002399928560685 1103 100 0.31973644331011997 1104 1 1.6044338889480265 1104 2 1.098549049046273 1104 3 0.6353912167953482 1104 4 0.5148474227854933 1104 5 0.3955020944711336 1104 6 0.3531301214474212 1104 7 0.3158653165180212 1104 8 0.33819734017511394 1104 9 0.41762201220418405 1104 10-11 0.38189261719249856 1104 12-13 0.37764884741975635 1104 14-15 0.5386841256449131 1104 16-17 0.49729434648472903 1104 18-19 0.4534611048552861 1104 20-21 0.43474313578946777 1104 22-23 0.42630502788748714 1104 24-25 0.4989744799587541 1104 26-27 0.4856001561542129 1104 28-29 0.49338324375310094 1104 30-31 0.46654090299083606 1104 32-33 0.49054417780896387 1104 34-35 0.47264295634646913 1104 36-37 0.5093737928790603 1104 38-39 0.5299549600521658 1104 40-41 0.5175904060744401 1104 42-43 0.48420381102528154 1104 44-45 0.5003414625909954 1104 46-47 0.5235039864537967 1104 48-49 0.5025232552554897 1104 50-51 0.4941987620619912 1104 52-53 0.46920839584715424 1104 54-55 0.4596632387768764 1104 56-57 0.5145801899174316 1104 58-59 0.4780055914818533 1104 60-61 0.45708984993828494 1104 62-63 0.5354723654299178 1104 64-65 0.4982575931675868 1104 66-67 0.4947760610032361 1104 68-69 0.5042502613614488 1104 70-71 0.5434433666589982 1104 72-73 0.46219194145843545 1104 74-75 0.41685420760949654 1104 76-77 0.4015346657403107 1104 78-79 0.5004393171632913 1104 80-81 0.43991634686982906 1104 82-83 0.431765305695059 1104 84-85 0.41880823533520584 1104 86-87 0.4245002030860263 1104 88-89 0.4580941520829853 1104 90-91 0.43371442100308144 1104 92-93 0.449039562261909 1104 94-95 0.4200254248775437 1104 96-97 0.4018675879087219 1104 98-99 0.4405215609252018 1104 100 0.36497682500223405 1105 1 1.583476319625273 1105 2 1.0422459047695618 1105 3 0.6071410710522684 1105 4 0.4832096347312458 1105 5 0.33362507132626007 1105 6 0.2712205168882278 1105 7 0.23270257104081793 1105 8 0.27679079457811184 1105 9 0.32410045941692545 1105 10-11 0.30598758794992165 1105 12-13 0.26905097476194584 1105 14-15 0.38301081248958013 1105 16-17 0.3268416539975476 1105 18-19 0.299718097454587 1105 20-21 0.3158627045203346 1105 22-23 0.29454923848660286 1105 24-25 0.3277399351291379 1105 26-27 0.2935862688391424 1105 28-29 0.32024993667143775 1105 30-31 0.3728539139223699 1105 32-33 0.38016190989949905 1105 34-35 0.3522572290282895 1105 36-37 0.38832212779209385 1105 38-39 0.4032759659342844 1105 40-41 0.4161012363625787 1105 42-43 0.3755682688684203 1105 44-45 0.38096063169018635 1105 46-47 0.3978352705650252 1105 48-49 0.39599052584452465 1105 50-51 0.3933590829356106 1105 52-53 0.3530996198959855 1105 54-55 0.4449201850826725 1105 56-57 0.45070077567957156 1105 58-59 0.45863187648966885 1105 60-61 0.4250088339754754 1105 62-63 0.474487160179315 1105 64-65 0.4484558283254856 1105 66-67 0.4364461037689651 1105 68-69 0.4553691383633591 1105 70-71 0.4556367294375221 1105 72-73 0.3655499761659442 1105 74-75 0.3103297794553228 1105 76-77 0.30732318238771583 1105 78-79 0.4407037458043348 1105 80-81 0.3906242645525282 1105 82-83 0.3750772944006684 1105 84-85 0.3861353881451919 1105 86-87 0.35097107820298135 1105 88-89 0.4125147438393526 1105 90-91 0.38485172310277704 1105 92-93 0.3864105369226465 1105 94-95 0.37504810284158907 1105 96-97 0.37856643565353565 1105 98-99 0.395465609308598 1105 100 0.31584710355421564 1106 1 1.4464195553059476 1106 2 0.903767676122083 1106 3 0.5110615628504007 1106 4 0.4519766956868523 1106 5 0.2822831389087881 1106 6 0.24205369284512557 1106 7 0.1996086421259875 1106 8 0.2028943914315704 1106 9 0.24961028698658083 1106 10-11 0.2289466932272859 1106 12-13 0.21774950573997387 1106 14-15 0.35043309905547204 1106 16-17 0.2628353895787683 1106 18-19 0.20312138293824233 1106 20-21 0.2199969345831292 1106 22-23 0.21069199202164413 1106 24-25 0.2758633061050233 1106 26-27 0.24959571465301877 1106 28-29 0.31015133364377334 1106 30-31 0.29988775355275976 1106 32-33 0.2545991973204238 1106 34-35 0.2648161523387458 1106 36-37 0.2719745250170291 1106 38-39 0.35600092307717546 1106 40-41 0.3390549211267171 1106 42-43 0.28958820382629114 1106 44-45 0.295559384428671 1106 46-47 0.33656880443598425 1106 48-49 0.30796058776142843 1106 50-51 0.2537232774649709 1106 52-53 0.2646000877297112 1106 54-55 0.25188626544986903 1106 56-57 0.26457633014153004 1106 58-59 0.20840439945485656 1106 60-61 0.24231712956902385 1106 62-63 0.3050436938123653 1106 64-65 0.28498390571181176 1106 66-67 0.25857112935413085 1106 68-69 0.24894205493991706 1106 70-71 0.2899511151461738 1106 72-73 0.27728586372389685 1106 74-75 0.22816777832809976 1106 76-77 0.15872975276409207 1106 78-79 0.261738965814466 1106 80-81 0.25289766992339224 1106 82-83 0.269935880057524 1106 84-85 0.2689237842788117 1106 86-87 0.2505041967496666 1106 88-89 0.27772671468206767 1106 90-91 0.27955967711142904 1106 92-93 0.232032847736086 1106 94-95 0.24791766997409326 1106 96-97 0.2707326115727078 1106 98-99 0.27826592036631226 1106 100 0.22016869671804784 1107 1 1.5935283471060124 1107 2 1.0404438102384468 1107 3 0.601374908205301 1107 4 0.5099331712745752 1107 5 0.36242642973353867 1107 6 0.33818297084239646 1107 7 0.2774660735003067 1107 8 0.287079750796714 1107 9 0.36651963532756326 1107 10-11 0.32344514504028865 1107 12-13 0.31367146864764095 1107 14-15 0.42195312852520317 1107 16-17 0.38611353100131396 1107 18-19 0.34561504551614775 1107 20-21 0.3454687819494424 1107 22-23 0.36900848441273837 1107 24-25 0.41008167670658935 1107 26-27 0.38689730888039975 1107 28-29 0.35394560096037253 1107 30-31 0.3648966388314605 1107 32-33 0.38370308870322845 1107 34-35 0.413914466030576 1107 36-37 0.4585516617845471 1107 38-39 0.47465710759556146 1107 40-41 0.4319104468101642 1107 42-43 0.42934719796409837 1107 44-45 0.39685974272441626 1107 46-47 0.4113503852730318 1107 48-49 0.4313258932307136 1107 50-51 0.4099754868665073 1107 52-53 0.3662339520304627 1107 54-55 0.38702126245902235 1107 56-57 0.3685864297780199 1107 58-59 0.35040446910529255 1107 60-61 0.3080180953587828 1107 62-63 0.36628979910459947 1107 64-65 0.3309281252467571 1107 66-67 0.3286594666309597 1107 68-69 0.31127396367133997 1107 70-71 0.3508254349737072 1107 72-73 0.3272252064354788 1107 74-75 0.32399688072062105 1107 76-77 0.2743225300946399 1107 78-79 0.3796244706779106 1107 80-81 0.3441181475718622 1107 82-83 0.3260209445718516 1107 84-85 0.31071972239787726 1107 86-87 0.29545912960446685 1107 88-89 0.35328891369340454 1107 90-91 0.36134656067375914 1107 92-93 0.3682344781542284 1107 94-95 0.34533907668828334 1107 96-97 0.3204107247472301 1107 98-99 0.35046548599645533 1107 100 0.2997866600342469 1108 1 1.4019312389291763 1108 2 0.8960147989112706 1108 3 0.4538772043632804 1108 4 0.3743558089572403 1108 5 0.22382803068995827 1108 6 0.1633263908005702 1108 7 0.12738440224436687 1108 8 0.1270795027421343 1108 9 0.13778650100620382 1108 10-11 0.12511169295172664 1108 12-13 0.11396884641584393 1108 14-15 0.16637663189691665 1108 16-17 0.13943562074248916 1108 18-19 0.0986191428667027 1108 20-21 0.10781621073414982 1108 22-23 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2316 48-49 -1.6389170385038483 2316 50-51 -1.428704524569227 2316 52-53 -1.2148268566347582 2316 54-55 -1.1413577586197547 2316 56-57 -1.2501688624846494 2316 58-59 -1.1022216112586065 2316 60-61 -1.071813924015018 2316 62-63 -2.1784200094555715 2316 64-65 -1.5354168624264943 2316 66-67 -1.2130832423088336 2316 68-69 -1.0790700183507056 2316 70-71 -1.0057860370128466 2316 72-73 -0.9531607643277411 2316 74-75 -1.070566445330826 2316 76-77 -0.9155342914372788 2316 78-79 -1.1454027737280725 2316 80-81 -1.046877918825814 2316 82-83 -0.9406596839067802 2316 84-85 -0.9044597647126551 2316 86-87 -1.0764726070686343 2316 88-89 -1.0352104692717532 2316 90-91 -0.9562278325633784 2316 92-93 -0.8918269710139768 2316 94-95 -0.904205525790001 2316 96-97 -0.8710176589444778 2316 98-99 -0.9143920715837552 2316 100 -0.8770616867867815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 36.0 7 787.0 8 984.0 9 393.0 10 172.0 11 188.0 12 240.0 13 1142.0 14 4993.0 15 12906.0 16 21880.0 17 29735.0 18 38455.0 19 49242.0 20 65823.0 21 93308.0 22 140703.0 23 224657.0 24 350798.0 25 427680.0 26 417540.0 27 377024.0 28 377354.0 29 425505.0 30 502719.0 31 613218.0 32 756422.0 33 954753.0 34 1279158.0 35 1864822.0 36 3110459.0 37 5916423.0 38 7907532.0 39 2441811.0 40 5990.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.373160282298905 33.76205266685039 27.694938012037746 20.169849038812966 2 21.297042124308796 31.774295357934644 28.10638605472941 18.82227646302715 3 23.897347767287332 27.348828000230302 27.416194179015964 21.337630053466405 4 20.055318253989146 27.701442893315086 27.293395721364305 24.94984313133146 5 20.70478443098535 31.96860275789497 26.964857356759392 20.361755454360292 6 25.290302409458263 27.04803459824461 27.19152645947267 20.470136532824455 7 20.510249358328526 27.43774980774139 31.721140057319317 20.330860776610766 8 24.78551005650144 27.024824904947597 27.477046158818634 20.712618879732332 9 24.893298757987548 26.81607139815474 27.643877926937645 20.646751916920067 10-11 20.18714368105806 29.72723032307189 29.677186071565675 20.408439924304375 12-13 20.259116605639896 27.323116094357978 29.525540023928333 22.892227276073793 14-15 20.253332658568834 32.178159858091114 27.04402613112326 20.524481352216792 16-17 22.827516398818474 27.22293081097167 26.99730584554866 22.95224694466119 18-19 20.40836282336306 29.674272724388512 26.92365442488402 22.993710027364408 20-21 22.877302673907614 29.645985928639668 26.899641739798376 20.57706965765434 22-23 22.86193149976639 27.272132193403646 29.32997856191544 20.535957744914526 24-25 20.544820364323336 29.629785326871634 26.75030384913105 23.07509045967398 26-27 20.57932970091653 27.19509234478964 31.606098191818326 20.6194797624755 28-29 20.570453789447857 27.263538096204055 31.588923989468604 20.577084124879484 30-31 20.605813818773367 27.26036369994114 31.53253094543656 20.60129153584893 32-33 20.637311431359908 27.280726290603237 31.485453452300227 20.596508825736628 34-35 20.67607109127297 27.294402237252548 31.454223305474194 20.575303366000288 36-37 20.706361953008976 27.280823551022255 31.380591589577723 20.632222906391046 38-39 20.74361851330424 27.285896122211017 31.345818728881643 20.624666635603102 40-41 20.735514659118863 27.34551181179959 31.341840564902512 20.577132964179036 42-43 20.778608043055147 27.37532400193673 31.262998694970502 20.58306926003762 44-45 20.81848349889988 27.402721807724106 31.202913145231715 20.5758815481443 46-47 20.860257885132995 27.4616684890983 31.12877528687681 20.5492983388919 48-49 20.896752687074994 27.484852267074928 31.029503860199338 20.58889118565074 50-51 20.90441822466645 27.54745300098695 30.957935307915736 20.590193466430865 52-53 20.998548273853622 27.686432431386145 30.72412804048644 20.590891254273792 54-55 21.313840487087827 27.883011248930558 30.074171292463447 20.728976971518172 56-57 21.637084117143672 28.292519003954723 29.122336775177505 20.948060103724096 58-59 21.732157579389266 28.70399383308278 28.544633473372222 21.01921511415573 60-61 21.767645074457928 28.92407614335516 28.23279131829005 21.07548746389686 62-63 21.791238122024964 29.02913082967819 28.063154536217354 21.11647651207949 64-65 21.757163189325773 29.112376299010943 27.9630490258444 21.167411485818878 66-67 21.70795842197927 29.047517448890364 27.97903977587714 21.265484353253225 68-69 21.673232396426727 28.958067288658967 28.044663761176693 21.324036553737617 70-71 21.630312869416674 28.889718466443288 28.083457066106206 21.396511598033836 72-73 21.56473658954699 28.86025072766558 28.106936266155707 21.468076416631725 74-75 21.566913978645424 28.864613113771743 28.01526853629626 21.553204371286576 76-77 21.55606073425112 28.905593086936832 27.89809499126984 21.640251187542205 78-79 21.583347298207336 28.95804489628831 27.625615544751597 21.83299226075276 80-81 21.566388191781243 29.06127088603434 27.40016337579634 21.972177546388075 82-83 21.51348782461499 29.175205091125335 27.192020507986275 22.1192865762734 84-85 21.476004753966066 29.219303256086665 27.01661882082074 22.288073169126534 86-87 21.448628902429682 29.21448996678088 26.902398780660043 22.434482350129397 88-89 21.49763428992359 29.224076658614674 26.722056913949814 22.556232137511927 90-91 21.459264845331543 29.152504117883836 26.616161841906273 22.77206919487835 92-93 21.40375078264487 29.114888923379816 26.584221034748907 22.89713925922641 94-95 21.372450394167007 29.08916200683196 26.56332606440648 22.975061534594552 96-97 21.36213064929197 29.000405901847355 26.503210313011106 23.134253135849573 98-99 21.320833455491357 28.90419482504466 26.51364351745988 23.2613282020041 100 21.32037558804107 28.886547869606368 26.46094541023943 23.332131132113137 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 46080.0 1 29016.0 2 9273.0 3 6222.5 4 5760.5 5 5649.0 6 5774.5 7 6004.5 8 6250.0 9 6574.5 10 7116.0 11 9039.5 12 73219.0 13 90561.0 14 44639.5 15 47397.0 16 54810.0 17 62890.0 18 71206.5 19 80405.0 20 92369.0 21 110035.5 22 134079.0 23 162329.5 24 193105.0 25 227775.0 26 268204.5 27 315647.0 28 371878.5 29 429222.5 30 481441.5 31 532011.0 32 585488.0 33 638982.0 34 687243.0 35 738039.5 36 796155.5 37 846550.5 38 886955.0 39 915368.5 40 929394.0 41 946399.0 42 969375.5 43 978511.5 44 982395.0 45 991630.5 46 992959.5 47 987956.5 48 973806.5 49 955426.5 50 937572.0 51 912798.0 52 880475.5 53 832556.0 54 770644.0 55 707645.0 56 642021.5 57 571013.5 58 498430.0 59 422903.0 60 344425.0 61 275468.0 62 216021.5 63 161112.0 64 118364.0 65 87888.0 66 63539.0 67 46633.5 68 35380.0 69 26791.5 70 21123.0 71 17787.5 72 15218.0 73 11946.5 74 8959.5 75 7064.5 76 5347.0 77 3464.0 78 2103.0 79 1415.5 80 953.0 81 654.5 82 471.5 83 339.0 84 234.5 85 163.0 86 110.5 87 70.0 88 46.0 89 34.5 90 27.0 91 21.0 92 15.0 93 10.5 94 5.5 95 2.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10723265424715217 2 0.0 3 0.0 4 0.0 5 0.00941409091273817 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.918394225667618E-5 20-21 0.010909787599808719 22-23 0.0 24-25 9.044565848873681E-4 26-27 0.00508888802236239 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.7596431612594708E-6 38-39 0.0 40-41 0.0076086970292859515 42-43 0.039695790074852404 44-45 0.015210355485926867 46-47 0.004747517249078052 48-49 0.0015889577746173022 50-51 0.0 52-53 0.0014147531016526146 54-55 6.158751064408148E-5 56-57 8.446287174045461E-4 58-59 2.2875361096373123E-5 60-61 2.850621921240343E-4 62-63 2.6746576051143956E-4 64-65 4.399107903148678E-5 66-67 2.7978326264025587E-4 68-69 4.8566151250761395E-4 70-71 4.293529313473109E-4 72-73 0.0012563852171392624 74-75 0.0016593435010676812 76-77 0.002477577571053335 78-79 0.004576831862435884 80-81 0.004288250383989331 82-83 0.007446809858450081 84-85 0.0024001532719579183 86-87 0.002783755481112483 88-89 0.0056554931202879395 90-91 0.005606223111772674 92-93 0.005567510962224966 94-95 0.002653541887179282 96-97 0.0055569531032574095 98-99 0.0077459491958641906 100 0.30357715746680647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8414852E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.80743096818699 #Duplication Level Percentage of deduplicated Percentage of total 1 69.23873034294378 32.408870908522545 2 17.41912833184826 16.30689293837955 3 5.691106462881245 7.991582186817501 4 2.422794143372449 4.536190784641346 5 1.279071466447046 2.9935024684548903 6 0.8169652398106025 2.2944026439505865 7 0.5611471564719676 1.838609975268923 8 0.4166135283554491 1.5600487175128397 9 0.3121922009895306 1.315162340696143 >10 1.606502232027152 13.40669422642181 >50 0.15820017577072223 5.30182219942613 >100 0.07688487442114056 4.982997045288741 >500 2.2916358437725603E-4 0.07226986451187135 >1k 3.973740293372037E-4 0.3289561046174853 >5k 0.0 0.0 >10k+ 3.7307046978929794E-5 4.66199759548955 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1202761 4.232860336559205 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 65919 0.23198783509412613 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0020974946482212892 3.519286322518942E-6 0.0 0.0 1.0557858967556826E-5 2 0.0021397260840915165 7.038572645037884E-6 0.0 0.0 1.0557858967556826E-5 3 0.002178438233639225 1.0557858967556826E-5 0.0 0.0 1.759643161259471E-5 4 0.002224188955831971 1.759643161259471E-5 0.0 0.0 2.1115717935113652E-5 5 0.0022558625327346415 1.759643161259471E-5 0.0 0.0 2.1115717935113652E-5 6 0.0022804975369922743 2.1115717935113652E-5 0.0 0.0 3.519286322518942E-5 7 0.0022875361096373124 2.1115717935113652E-5 0.0 0.0 4.2231435870227304E-5 8 0.0023332868318300583 2.1115717935113652E-5 0.0 0.0 4.2231435870227304E-5 9 0.002354402549765172 2.8154290580151536E-5 0.0 3.519286322518942E-6 4.5750722192746246E-5 10-11 0.0024195093467317724 3.3433220063929946E-5 0.0 3.519286322518942E-6 4.5750722192746246E-5 12-13 0.002514530077439784 3.871214954770836E-5 0.0 1.0557858967556826E-5 5.630858116030307E-5 14-15 0.0026095508081477956 5.102965167652466E-5 0.0 1.583678845133524E-5 6.334715380534096E-5 16-17 0.00269401367988825 5.630858116030307E-5 0.0 1.759643161259471E-5 6.334715380534096E-5 18-19 0.0028295062033052293 6.686644012785989E-5 0.0 1.935607477385418E-5 6.334715380534096E-5 20-21 0.0029262865771745 7.91839422566762E-5 3.519286322518942E-6 2.4635004257632594E-5 6.686644012785989E-5 22-23 0.003081135175365334 8.974180122423302E-5 3.519286322518942E-6 3.167357690267048E-5 7.038572645037884E-5 24-25 0.003746280290321414 9.854001703053038E-5 3.519286322518942E-6 3.167357690267048E-5 7.038572645037884E-5 26-27 0.005641415974997864 1.0557858967556826E-4 3.519286322518942E-6 3.519286322518942E-5 7.390501277289778E-5 28-29 0.010923864745098795 1.1085751915934667E-4 3.519286322518942E-6 3.871214954770836E-5 7.566465593415725E-5 30-31 0.024561099244859694 1.214153781269035E-4 3.519286322518942E-6 4.7510365354005714E-5 7.91839422566762E-5 32-33 0.05268899517759234 1.266943076106819E-4 3.519286322518942E-6 4.927000851526519E-5 8.798215806297354E-5 34-35 0.10079763920642627 1.3197323709446032E-4 3.519286322518942E-6 5.102965167652466E-5 9.502073070801143E-5 36-37 0.175760197519241 1.4077145290075768E-4 3.519286322518942E-6 5.630858116030307E-5 9.502073070801143E-5 38-39 0.28193002729699246 1.49569668707055E-4 3.519286322518942E-6 6.510679696660043E-5 1.0029966019178984E-4 40-41 0.4230199756099381 1.5484859819083345E-4 3.519286322518942E-6 6.686644012785989E-5 1.0205930335304931E-4 42-43 0.6013686082193918 1.6012752767461186E-4 3.519286322518942E-6 6.686644012785989E-5 1.0205930335304931E-4 44-45 0.8199514817110432 1.6716610031964973E-4 3.519286322518942E-6 7.21453696116383E-5 1.0381894651430879E-4 46-47 1.0809364764595641 1.7244502980342814E-4 3.519286322518942E-6 8.094358541793566E-5 1.1261716232060614E-4 48-49 1.3837006787858688 1.812432456097255E-4 3.519286322518942E-6 8.446287174045461E-5 1.1965573496564403E-4 50-51 1.7284710826577594 1.9180110457728234E-4 3.519286322518942E-6 8.974180122423302E-5 1.3021359393320086E-4 52-53 2.1157650231646463 1.9708003406106075E-4 3.519286322518942E-6 9.326108754675197E-5 1.3373288025571978E-4 54-55 2.539715850006891 1.9708003406106075E-4 3.519286322518942E-6 9.854001703053038E-5 1.3725216657823873E-4 56-57 3.002903200058899 2.1291682251239598E-4 3.519286322518942E-6 9.854001703053038E-5 1.3725216657823873E-4 58-59 3.5058232926921455 2.287536109637312E-4 3.519286322518942E-6 1.0557858967556826E-4 1.4077145290075768E-4 60-61 4.0434417888222685 2.42830756253807E-4 3.519286322518942E-6 1.1789609180438455E-4 1.4077145290075768E-4 62-63 4.607917718522693 2.5162897206010434E-4 3.519286322518942E-6 1.266943076106819E-4 1.460503823845361E-4 64-65 5.204280141948303 2.6746576051143956E-4 3.519286322518942E-6 1.3021359393320086E-4 1.5484859819083345E-4 66-67 5.834249990110806 2.780236194789964E-4 3.519286322518942E-6 1.3549252341697927E-4 1.6188717083587132E-4 68-69 6.490318865641109 2.8154290580151536E-4 3.519286322518942E-6 1.3725216657823873E-4 1.6540645715839027E-4 70-71 7.175052328268329 2.850621921240343E-4 3.519286322518942E-6 1.390118097394982E-4 1.7244502980342814E-4 72-73 7.884088222595705 2.938604079303316E-4 3.519286322518942E-6 1.4077145290075768E-4 1.7772395928720655E-4 74-75 8.617284017527172 3.02658623736629E-4 3.519286322518942E-6 1.4077145290075768E-4 1.865221750935039E-4 76-77 9.372660114506315 3.02658623736629E-4 3.519286322518942E-6 1.460503823845361E-4 1.9004146141602285E-4 78-79 10.146989327975383 3.1497612586544525E-4 3.519286322518942E-6 1.4781002554579555E-4 1.988396772223202E-4 80-81 10.943224339158974 3.2905327115552105E-4 3.519286322518942E-6 1.49569668707055E-4 2.1115717935113652E-4 82-83 11.758190751794167 3.448900596068563E-4 3.519286322518942E-6 1.566082413520929E-4 2.1291682251239598E-4 84-85 12.586889419659832 3.5368827541315366E-4 3.519286322518942E-6 1.6188717083587132E-4 2.234746814799528E-4 86-87 13.431315778100831 3.62486491219451E-4 7.038572645037884E-6 1.636468139971308E-4 2.3227289728625016E-4 88 14.074977409701095 3.62486491219451E-4 7.038572645037884E-6 1.6540645715839027E-4 2.3227289728625016E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 152675 0.0 85.55615 8 GTATCAA 155215 0.0 84.02286 9 AGTGGTA 156255 0.0 83.98036 5 GTGGTAT 156385 0.0 83.82395 6 TGGTATC 156115 0.0 83.76423 7 GCAGTGG 164375 0.0 80.01478 3 AAGCAGT 163345 0.0 78.79627 1 CAGTGGT 166675 0.0 78.68224 4 AGCAGTG 171705 0.0 76.4813 2 CAACGCA 148155 0.0 44.834957 12-13 ATCAACG 149585 0.0 44.428295 10-11 TCAACGC 147705 0.0 44.193657 12-13 ACGCAGA 151010 0.0 44.118885 14-15 AACGCAG 149760 0.0 43.649143 14-15 CGCAGAG 152570 0.0 42.97769 16-17 AGAGTAC 154380 0.0 42.94488 18-19 GAGTACT 150640 0.0 42.87723 20-21 TATCAAC 156255 0.0 41.748352 10-11 AGTACTT 160625 0.0 40.947777 20-21 GTACTTT 160100 0.0 40.32519 22-23 >>END_MODULE