FastQCFastQC Report
Thu 26 May 2016
SRR1660752_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660752_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28403735
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12567304.424523746612902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1112200.3915682215736768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1582350.085.399958
AGTGGTA1609200.084.109595
TGGTATC1605000.083.9840557
GTATCAA1612650.083.713819
GTGGTAT1622150.083.4059756
GCAGTGG1702400.079.961913
AAGCAGT1719850.079.022471
CAGTGGT1732350.078.146644
AGCAGTG1800200.075.733842
CAACGCA1548200.044.40032612-13
ATCAACG1560100.044.1339210-11
TCAACGC1544500.043.88628812-13
AGAGTAC1554000.043.73476818-19
ACGCAGA1575900.043.6721914-15
GAGTACT1525000.043.5414820-21
AACGCAG1562300.043.51247814-15
CGCAGAG1588400.042.76189816-17
TATCAAC1609750.041.96003310-11
AGTACTT1625600.041.43561620-21
GTACTTT1622500.040.9686622-23
Produced by FastQC (version 0.11.5)