FastQCFastQC Report
Thu 26 May 2016
SRR1660752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28403735
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13702134.824059230238559No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA851800.29989013768787803No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293020.10316248901772952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1718950.085.604638
AGTGGTA1748800.084.453355
TGGTATC1744350.084.4227757
GTATCAA1754200.083.729069
GTGGTAT1765000.083.6453866
GCAGTGG1837350.080.55713
AAGCAGT1816150.079.828651
CAGTGGT1870650.078.8567664
AGCAGTG1921850.076.885552
CAACGCA1681000.044.4730912-13
ATCAACG1692700.044.18651610-11
ACGCAGA1706050.043.91299414-15
TCAACGC1677950.043.8523712-13
AGAGTAC1706500.043.62812418-19
AACGCAG1691650.043.55882614-15
GAGTACT1671300.043.4975920-21
CGCAGAG1718550.042.9563316-17
TATCAAC1748300.042.03806310-11
AGTACTT1770800.041.76220320-21
GTACTTT1765750.041.17616322-23
Produced by FastQC (version 0.11.5)