##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660752_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28403735 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.976481226852737 33.0 31.0 34.0 26.0 34.0 2 31.76428564764458 34.0 31.0 34.0 28.0 34.0 3 32.2753520267669 34.0 31.0 34.0 30.0 34.0 4 35.81012384462818 37.0 35.0 37.0 35.0 37.0 5 35.86786466638983 37.0 35.0 37.0 35.0 37.0 6 35.908445244965144 37.0 35.0 37.0 35.0 37.0 7 35.90835888308351 37.0 35.0 37.0 35.0 37.0 8 35.91159990050604 37.0 35.0 37.0 35.0 37.0 9 37.62205002264667 39.0 37.0 39.0 35.0 39.0 10-11 37.56634428535543 39.0 37.0 39.0 35.0 39.0 12-13 37.620147755215996 39.0 37.0 39.0 35.0 39.0 14-15 38.943335445144804 40.0 38.0 41.0 35.0 41.0 16-17 38.95735203486443 40.0 38.0 41.0 35.0 41.0 18-19 38.9414063502564 40.0 38.0 41.0 35.0 41.0 20-21 38.88263812840107 40.0 38.0 41.0 35.0 41.0 22-23 38.787593726670096 40.0 38.0 41.0 34.5 41.0 24-25 38.726778643724145 40.0 38.0 41.0 34.0 41.0 26-27 38.67596738597934 40.0 38.0 41.0 34.0 41.0 28-29 38.57022741903485 40.0 38.0 41.0 34.0 41.0 30-31 38.47278051636519 40.0 38.0 41.0 34.0 41.0 32-33 38.369399253302426 40.0 38.0 41.0 34.0 41.0 34-35 38.26253879991487 40.0 38.0 41.0 34.0 41.0 36-37 38.08528689976864 40.0 38.0 41.0 33.0 41.0 38-39 37.70197764484143 40.0 36.0 41.0 33.0 41.0 40-41 37.55773446696359 40.0 36.0 41.0 33.0 41.0 42-43 37.40633330440521 40.0 36.0 41.0 32.5 41.0 44-45 37.295937382882926 39.5 35.5 41.0 32.0 41.0 46-47 37.16856348293631 39.0 35.0 41.0 31.5 41.0 48-49 37.297265166007215 40.0 35.5 41.0 32.0 41.0 50-51 37.216412471810486 40.0 35.0 41.0 31.5 41.0 52-53 36.98204468532043 39.0 35.0 41.0 31.0 41.0 54-55 36.53782453258348 39.0 35.0 41.0 29.5 41.0 56-57 36.28599860194443 39.0 35.0 41.0 28.5 41.0 58-59 36.10531025233125 39.0 35.0 41.0 28.0 41.0 60-61 35.916154283934844 39.0 35.0 41.0 28.0 41.0 62-63 35.63640445525914 38.5 35.0 41.0 27.5 41.0 64-65 35.39777816192131 38.0 35.0 40.0 27.0 41.0 66-67 35.1356572471895 37.0 34.5 40.0 26.5 41.0 68-69 34.84493146763973 37.0 34.0 40.0 26.0 41.0 70-71 34.509703195724086 36.0 34.0 39.0 26.0 41.0 72-73 34.188398444781996 36.0 34.0 39.0 26.0 41.0 74-75 33.829514903585746 35.0 34.0 38.5 26.0 40.0 76-77 31.7296149256427 34.0 31.0 36.0 22.5 38.5 78-79 32.988539060091924 35.0 33.0 37.0 25.0 39.0 80-81 32.967105857733145 35.0 33.0 36.5 25.0 39.0 82-83 32.745116232073 35.0 33.0 36.0 25.0 37.5 84-85 32.50826026929205 35.0 33.0 36.0 25.0 37.0 86-87 32.29893570335028 35.0 33.0 35.0 25.0 37.0 88-89 32.07961370221206 35.0 33.0 35.0 24.0 36.0 90-91 31.923492051309452 35.0 33.0 35.0 24.0 36.0 92-93 31.799001310919145 35.0 33.0 35.0 24.0 36.0 94-95 31.701450795115502 35.0 33.0 35.0 23.0 35.5 96-97 31.599925625978415 35.0 33.0 35.0 23.0 35.0 98-99 31.48745693128034 35.0 33.0 35.0 21.5 35.0 100 31.30512596318759 35.0 32.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7470895572994642 1101 2 1.2199935474654744 1101 3 0.7690168511407336 1101 4 0.6083068297893348 1101 5 0.4662321703332779 1101 6 0.4200033875413709 1101 7 0.38868225015898616 1101 8 0.41646109543899 1101 9 0.5113337066102588 1101 10-11 0.5017051551389642 1101 12-13 0.4564990720840356 1101 14-15 0.622249760193192 1101 16-17 0.6092621258581943 1101 18-19 0.5691131136257752 1101 20-21 0.6081194437710309 1101 22-23 0.5716411657699894 1101 24-25 0.6151962520501186 1101 26-27 0.6127440479380013 1101 28-29 0.6133223317400223 1101 30-31 0.5999585547172757 1101 32-33 0.5915875336610554 1101 34-35 0.5915963138294771 1101 36-37 0.5800689833762434 1101 38-39 0.6670097027195112 1101 40-41 0.6598981738672833 1101 42-43 0.6070185777566905 1101 44-45 0.5743436722965498 1101 46-47 0.5408989234888253 1101 48-49 0.5492389747697146 1101 50-51 0.5505484714201216 1101 52-53 0.5628286432046608 1101 54-55 0.6059986525138044 1101 56-57 0.6773287261588194 1101 58-59 0.6653784273118291 1101 60-61 0.6525464893697688 1101 62-63 0.6818632851653277 1101 64-65 0.6503217421075647 1101 66-67 0.6154788403072828 1101 68-69 0.6260160141448097 1101 70-71 0.6095447100363955 1101 72-73 0.5707295728936188 1101 74-75 0.5990632323988478 1101 76-77 0.49760888284865246 1101 78-79 0.6178762595034968 1101 80-81 0.5994153552226393 1101 82-83 0.5364870330082283 1101 84-85 0.5443398314537333 1101 86-87 0.513012011097068 1101 88-89 0.532523951064185 1101 90-91 0.5555137570845581 1101 92-93 0.5044926394825247 1101 94-95 0.45677392478989276 1101 96-97 0.4599739254250821 1101 98-99 0.4823214145764467 1101 100 0.40919667971560614 1102 1 1.6598815255920236 1102 2 1.1350650999114897 1102 3 0.704628277611576 1102 4 0.5568198613864581 1102 5 0.4152444238300035 1102 6 0.3989656741210652 1102 7 0.36111351743825537 1102 8 0.36298408044948616 1102 9 0.46833121711021874 1102 10-11 0.4570541103410122 1102 12-13 0.3936558904414085 1102 14-15 0.5468556689215021 1102 16-17 0.5379807783496773 1102 18-19 0.48658107948559604 1102 20-21 0.5090670624961149 1102 22-23 0.5139974391299376 1102 24-25 0.5555364198124622 1102 26-27 0.5327884770917422 1102 28-29 0.5294287966393583 1102 30-31 0.525443719895506 1102 32-33 0.5333609887404052 1102 34-35 0.5248507231463435 1102 36-37 0.5528991456608239 1102 38-39 0.6099696519546356 1102 40-41 0.5759260216138529 1102 42-43 0.5709277933485595 1102 44-45 0.5681487640963638 1102 46-47 0.5692002616613863 1102 48-49 0.5302986741940074 1102 50-51 0.5455108162216291 1102 52-53 0.48957390641138687 1102 54-55 0.5240120979759624 1102 56-57 0.5743349448002988 1102 58-59 0.55866818627171 1102 60-61 0.47595992512273 1102 62-63 0.5448319244604463 1102 64-65 0.5141589549435608 1102 66-67 0.4845816308324373 1102 68-69 0.4426983046120583 1102 70-71 0.4528621019677743 1102 72-73 0.3966793750643447 1102 74-75 0.4089497889285454 1102 76-77 0.3591317854792706 1102 78-79 0.43043797003117135 1102 80-81 0.4373980459611033 1102 82-83 0.42044003162043 1102 84-85 0.3973608146876373 1102 86-87 0.3869349797276058 1102 88-89 0.42168966616251424 1102 90-91 0.4465841482883981 1102 92-93 0.4212684653776826 1102 94-95 0.3606924325120815 1102 96-97 0.35463277209222355 1102 98-99 0.45456523336516597 1102 100 0.2961021810415758 1103 1 1.6492002729124842 1103 2 1.0808807683432953 1103 3 0.65903165456659 1103 4 0.5432882065761575 1103 5 0.4003448049805485 1103 6 0.34401702986107807 1103 7 0.3197625697800319 1103 8 0.3406633025634278 1103 9 0.4223430559760857 1103 10-11 0.3848493794382435 1103 12-13 0.3820453844047691 1103 14-15 0.5188578679517732 1103 16-17 0.4645442913747928 1103 18-19 0.4315034786214511 1103 20-21 0.44363257227511355 1103 22-23 0.4733093052572457 1103 24-25 0.5051857561371662 1103 26-27 0.42203949477848113 1103 28-29 0.46118848249683 1103 30-31 0.4755536614023299 1103 32-33 0.46362887977884526 1103 34-35 0.4664292159248902 1103 36-37 0.48240972521205805 1103 38-39 0.5316564814180182 1103 40-41 0.49955814416174604 1103 42-43 0.42650690979918693 1103 44-45 0.48545844223265533 1103 46-47 0.4534180822279694 1103 48-49 0.47972819077089923 1103 50-51 0.3996029053862742 1103 52-53 0.37515362427485144 1103 54-55 0.4246612211625589 1103 56-57 0.46465559419213776 1103 58-59 0.459299511905634 1103 60-61 0.45999066649854825 1103 62-63 0.510445153917253 1103 64-65 0.4840383170874034 1103 66-67 0.4564045083229402 1103 68-69 0.4686321309436039 1103 70-71 0.4576220140554952 1103 72-73 0.39518183063920986 1103 74-75 0.3645600029610492 1103 76-77 0.2238170054305968 1103 78-79 0.3886957659136243 1103 80-81 0.47419673309547505 1103 82-83 0.4296409636773362 1103 84-85 0.4319703528117813 1103 86-87 0.37800278789166697 1103 88-89 0.45112149152298997 1103 90-91 0.44279787409606897 1103 92-93 0.4024449215203454 1103 94-95 0.3821697145344558 1103 96-97 0.3857923238280492 1103 98-99 0.4401535934716634 1103 100 0.35491750763448593 1104 1 1.59640920758714 1104 2 1.0843372315510216 1104 3 0.6287066545090383 1104 4 0.5191854408732866 1104 5 0.3972256035267705 1104 6 0.34342970536486916 1104 7 0.2895306114062919 1104 8 0.3096228319565526 1104 9 0.38173356630316846 1104 10-11 0.3733350299955944 1104 12-13 0.35348433207897045 1104 14-15 0.515693973379264 1104 16-17 0.484150182837773 1104 18-19 0.41045357406274263 1104 20-21 0.3904040436707632 1104 22-23 0.3846520876838184 1104 24-25 0.4509768111366341 1104 26-27 0.4394364634394563 1104 28-29 0.4587610319748521 1104 30-31 0.42796984136745664 1104 32-33 0.437776271533572 1104 34-35 0.41365386146637206 1104 36-37 0.4472882644265681 1104 38-39 0.4664008258125989 1104 40-41 0.4847669075802514 1104 42-43 0.45882852372915295 1104 44-45 0.42710232081641664 1104 46-47 0.43180783097150055 1104 48-49 0.43856515927043205 1104 50-51 0.41911882906352815 1104 52-53 0.39103898278197136 1104 54-55 0.43273607803386227 1104 56-57 0.4663601726411315 1104 58-59 0.45854885541918833 1104 60-61 0.4226135853388868 1104 62-63 0.508692282792218 1104 64-65 0.4710879678312594 1104 66-67 0.48861926690913293 1104 68-69 0.507243887452475 1104 70-71 0.51708598344743 1104 72-73 0.44689769928175593 1104 74-75 0.39948298962411144 1104 76-77 0.3597971302671752 1104 78-79 0.4637534194023587 1104 80-81 0.41438227285896545 1104 82-83 0.37695678239554553 1104 84-85 0.40431599166456067 1104 86-87 0.44461967583761464 1104 88-89 0.45681360760669776 1104 90-91 0.4523356617533416 1104 92-93 0.4430539640808142 1104 94-95 0.44569214779436095 1104 96-97 0.4262641376085128 1104 98-99 0.4261089455596405 1104 100 0.3705028083728621 1105 1 1.5909717647409956 1105 2 1.0507943275491876 1105 3 0.6059892506353961 1105 4 0.5056044370981354 1105 5 0.3626637146488534 1105 6 0.2817522595238273 1105 7 0.23359309828575903 1105 8 0.27195217001786176 1105 9 0.3281820590461777 1105 10-11 0.31073780732431544 1105 12-13 0.28075091552046416 1105 14-15 0.3879819614160951 1105 16-17 0.3426187258840159 1105 18-19 0.3337551895331359 1105 20-21 0.361394090173647 1105 22-23 0.3276431366838324 1105 24-25 0.38556744711492286 1105 26-27 0.34184393999081664 1105 28-29 0.3569869022236034 1105 30-31 0.3845152109579715 1105 32-33 0.40803314942522917 1105 34-35 0.38175219628723767 1105 36-37 0.40832595811614425 1105 38-39 0.443045698333691 1105 40-41 0.4256650905535082 1105 42-43 0.3886477395529724 1105 44-45 0.40863647167952877 1105 46-47 0.45772164248886327 1105 48-49 0.39817316411209447 1105 50-51 0.3917070634373019 1105 52-53 0.37417917355019625 1105 54-55 0.4781067511257433 1105 56-57 0.4577340001119552 1105 58-59 0.4503731165460323 1105 60-61 0.43444093762337843 1105 62-63 0.4509381111892452 1105 64-65 0.4043679764064976 1105 66-67 0.4515097355257751 1105 68-69 0.483069370173304 1105 70-71 0.4931742596473967 1105 72-73 0.44031955462630634 1105 74-75 0.3452068142313962 1105 76-77 0.3256580868169294 1105 78-79 0.4241196088176409 1105 80-81 0.39292128600515497 1105 82-83 0.39203274496637874 1105 84-85 0.38755282653495016 1105 86-87 0.40806761946530656 1105 88-89 0.4549625172398848 1105 90-91 0.39993527783172667 1105 92-93 0.3572130376389744 1105 94-95 0.3769471346371063 1105 96-97 0.3959607969237311 1105 98-99 0.4369080482334411 1105 100 0.4240970836254583 1106 1 1.434558715870093 1106 2 0.9109059194254918 1106 3 0.5018184688991951 1106 4 0.4473415371237337 1106 5 0.25838881250104606 1106 6 0.2509930013210493 1106 7 0.1876453817917465 1106 8 0.20036509901910904 1106 9 0.2540913472375337 1106 10-11 0.2402877829483785 1106 12-13 0.19979457701568748 1106 14-15 0.3258115749112207 1106 16-17 0.25774105454462415 1106 18-19 0.20359127047561287 1106 20-21 0.22639183351330416 1106 22-23 0.22452736625760394 1106 24-25 0.24569383355819951 1106 26-27 0.25098810567590846 1106 28-29 0.2720039999984962 1106 30-31 0.2797962999724888 1106 32-33 0.2577770594781654 1106 34-35 0.2714914052903765 1106 36-37 0.25971499516442265 1106 38-39 0.32358417671667894 1106 40-41 0.32534385706657076 1106 42-43 0.29865661706253377 1106 44-45 0.2524494780215747 1106 46-47 0.3300869275859739 1106 48-49 0.30886490818207335 1106 50-51 0.3280835459090099 1106 52-53 0.2689861894999481 1106 54-55 0.29851397223879417 1106 56-57 0.27597687513505775 1106 58-59 0.2269539344599636 1106 60-61 0.2691610357646681 1106 62-63 0.29962116776126635 1106 64-65 0.2925626589050907 1106 66-67 0.2395379353873679 1106 68-69 0.22909279445767083 1106 70-71 0.27598834567547215 1106 72-73 0.2679396865421708 1106 74-75 0.23561037013874397 1106 76-77 0.15289250593876602 1106 78-79 0.27540050287085904 1106 80-81 0.26288900557437955 1106 82-83 0.2464418580400718 1106 84-85 0.3026345055600004 1106 86-87 0.2697388151608635 1106 88-89 0.2927710073632497 1106 90-91 0.31761614588497267 1106 92-93 0.29093860235192537 1106 94-95 0.2778466321270585 1106 96-97 0.31118077998477034 1106 98-99 0.3370248519006793 1106 100 0.27489353655573723 1107 1 1.5698107614125298 1107 2 1.0498207555561017 1107 3 0.592357356490723 1107 4 0.5019290277701671 1107 5 0.3308565474198417 1107 6 0.313302626513142 1107 7 0.2587118636470933 1107 8 0.26364134266621875 1107 9 0.3280992340481319 1107 10-11 0.3041202378943453 1107 12-13 0.2963498609622164 1107 14-15 0.38509114032840586 1107 16-17 0.3857662413647631 1107 18-19 0.32862737428383326 1107 20-21 0.3512976740595519 1107 22-23 0.37960783357161176 1107 24-25 0.4119238175629718 1107 26-27 0.36174771928590843 1107 28-29 0.3235806127346521 1107 30-31 0.37129392375965864 1107 32-33 0.3715734930088601 1107 34-35 0.4105374384697882 1107 36-37 0.4501066779330216 1107 38-39 0.4719510934184967 1107 40-41 0.4758539793157439 1107 42-43 0.4357908708114877 1107 44-45 0.45169434658834007 1107 46-47 0.4985320030296734 1107 48-49 0.4830490256766211 1107 50-51 0.46747010917173526 1107 52-53 0.4029694502333214 1107 54-55 0.44043567648920856 1107 56-57 0.4000729184841134 1107 58-59 0.4018852579701715 1107 60-61 0.35564500900092355 1107 62-63 0.41675527712638427 1107 64-65 0.39769716815067824 1107 66-67 0.40303148456826676 1107 68-69 0.34304814045786003 1107 70-71 0.38584543470255284 1107 72-73 0.3623677020929392 1107 74-75 0.33929847498150423 1107 76-77 0.2736538670641053 1107 78-79 0.37252122612720484 1107 80-81 0.3683455614170299 1107 82-83 0.35801262636928755 1107 84-85 0.3581490464256447 1107 86-87 0.3039219219684952 1107 88-89 0.3531802429700619 1107 90-91 0.3748222729703805 1107 92-93 0.3618328907781212 1107 94-95 0.3249800595923773 1107 96-97 0.31090089111638974 1107 98-99 0.3370394982293128 1107 100 0.2858320566589896 1108 1 1.3928490578379957 1108 2 0.8817380094745566 1108 3 0.4529612891307977 1108 4 0.37363227996501536 1108 5 0.20854142395789665 1108 6 0.1706930715729129 1108 7 0.13146036095002955 1108 8 0.14601664950328797 1108 9 0.1684373155585206 1108 10-11 0.1231998878418139 1108 12-13 0.09868236904387828 1108 14-15 0.16534237025933152 1108 16-17 0.12900588260875168 1108 18-19 0.10775802062237005 1108 20-21 0.09992529955780327 1108 22-23 0.06824052575552031 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44-45 -1.8235496787976757 2316 46-47 -1.657651480916826 2316 48-49 -1.6228161620558836 2316 50-51 -1.4446148920854611 2316 52-53 -1.2524123610212712 2316 54-55 -1.1756187580988993 2316 56-57 -1.3589668605177678 2316 58-59 -1.2113229132138201 2316 60-61 -1.1308138221201744 2316 62-63 -2.190754460763138 2316 64-65 -1.505747094619835 2316 66-67 -1.2101397922828028 2316 68-69 -1.1901612526500074 2316 70-71 -1.1368238850166676 2316 72-73 -1.0420527827621697 2316 74-75 -1.1213365084535454 2316 76-77 -0.9639879064639771 2316 78-79 -1.2504774906964684 2316 80-81 -1.0949110625979301 2316 82-83 -0.9902279483429872 2316 84-85 -0.9527227388921915 2316 86-87 -1.1042709838727376 2316 88-89 -1.0617998428405144 2316 90-91 -0.9925870975115849 2316 92-93 -0.9308337087430054 2316 94-95 -0.9663671545640469 2316 96-97 -0.9564481934719957 2316 98-99 -1.0206091581633707 2316 100 -0.9833424246059757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 34.0 7 806.0 8 958.0 9 415.0 10 180.0 11 185.0 12 270.0 13 1278.0 14 5527.0 15 15048.0 16 24818.0 17 33427.0 18 42515.0 19 53036.0 20 71089.0 21 101033.0 22 152252.0 23 238113.0 24 367315.0 25 460210.0 26 461410.0 27 410371.0 28 394314.0 29 433101.0 30 510211.0 31 619506.0 32 760425.0 33 961443.0 34 1284665.0 35 1875710.0 36 3128361.0 37 5959037.0 38 7847718.0 39 2184676.0 40 4278.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.440426385637902 34.17032481850803 27.872063162394806 19.517185633459267 2 21.31471089981652 32.703903201462765 27.182129392490108 18.79925650623061 3 25.138130601486036 26.857971319616947 27.01538371626126 20.98851436263576 4 20.478887723744784 27.297681097221897 26.637035587045155 25.586395591988165 5 20.734505910772793 32.314736257396916 26.50628019609434 20.44447763573595 6 26.13435169705674 26.754030763911857 26.600829081105005 20.510788457926395 7 20.559616543387694 26.961725280143618 32.04475749404084 20.433900682427858 8 25.761281042792433 26.901303648974334 26.71403954444724 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20.59871527459329 27.059152607922865 31.77096955734871 20.571162560135136 34-35 20.63641982295638 27.059895467972783 31.756696786531773 20.54698792253906 36-37 20.68358087413504 27.035032540614818 31.680192763381292 20.60119382186885 38-39 20.707153842745573 27.037272158701526 31.65221108513037 20.60336291342253 40-41 20.714327814493988 27.111410953120146 31.642025118000294 20.532236114385572 42-43 20.741976925237424 27.148896765309257 31.564320328786827 20.544805980666492 44-45 20.79747279403688 27.177226624629196 31.478119813440596 20.547180767893323 46-47 20.846204037002845 27.2457800862872 31.385010357238645 20.523005519471305 48-49 20.887319265317732 27.25669294820493 31.29308009466445 20.562907691812896 50-51 20.8883549997914 27.355208742793863 31.17601083096994 20.580425426444794 52-53 20.96173577038927 27.504800315399503 30.965739876666625 20.567724037544597 54-55 21.296477431291258 27.718393632157706 30.262748872123908 20.722380064427128 56-57 21.618771526390333 28.164184860546097 29.236960800757426 20.98008281230614 58-59 21.726517730348636 28.615835217123614 28.59722322365571 21.06042382887204 60-61 21.762964246574455 28.832726674340798 28.251945805849783 21.15236327323497 62-63 21.774738599699425 28.9790903348051 28.063445980674533 21.182725084820937 64-65 21.754563962365772 29.098878886207785 27.952315960889347 21.194241190537095 66-67 21.748509276831328 28.989404324737823 27.9804094992815 21.28167689914935 68-69 21.70525650417736 28.886573571398998 28.063359028674967 21.34481089574868 70-71 21.64863592568004 28.81536477338576 28.157482104130317 21.378517196803887 72-73 21.606987854919947 28.738260492069003 28.185933903076265 21.468817749934782 74-75 21.613535189693764 28.78501492073104 28.092150950314114 21.509298939261086 76-77 21.660486663845724 28.86077366134946 27.908750383695796 21.569989291109017 78-79 21.716570749716162 29.01525293491116 27.53823171255859 21.72994460281408 80-81 21.779839332847637 29.179068080520707 27.18950430697008 21.851588279661577 82-83 21.75776011864679 29.35176055062932 26.91583539194598 21.97464393877791 84-85 21.77788487883111 29.420060822976886 26.698917744208185 22.103136553983823 86-87 21.815156398177184 29.41620697146497 26.557998682426454 22.210637947931396 88-89 21.91250205794584 29.409697336549804 26.388463842190866 22.289336763313482 90-91 21.91990870534382 29.358675426136177 26.26248012889047 22.458935739629542 92-93 21.952436098276156 29.328751309077905 26.210307646268383 22.50850494637756 94-95 21.971763372551898 29.331728221338572 26.16584637486829 22.53066203124124 96-97 21.995156597610784 29.282115927772555 26.08274450009342 22.639982974523242 98-99 21.981666172502436 29.237493499020424 26.078248925610907 22.702591402866236 100 21.965965575449946 29.21431591097624 26.03904814462272 22.780670368951096 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 55128.0 1 34694.0 2 11145.5 3 7639.5 4 7064.0 5 7093.0 6 7292.5 7 7264.5 8 7466.5 9 7864.5 10 8511.5 11 10735.5 12 91101.0 13 111767.5 14 52438.5 15 55017.0 16 63275.5 17 72256.0 18 80909.0 19 89932.5 20 101646.5 21 118831.0 22 141862.0 23 169335.5 24 198886.5 25 230219.0 26 265005.0 27 306248.0 28 361623.5 29 419569.5 30 466057.0 31 508112.5 32 551565.0 33 591536.5 34 629487.5 35 674245.0 36 724037.0 37 769190.5 38 821631.5 39 864466.0 40 886424.0 41 925143.0 42 976824.0 43 993378.0 44 985237.5 45 995127.5 46 1009404.0 47 1014510.0 48 1006304.5 49 990924.5 50 979246.5 51 966308.5 52 942884.5 53 886153.5 54 809608.5 55 737389.0 56 664594.0 57 589874.5 58 513363.0 59 430953.5 60 347184.0 61 276344.0 62 214808.0 63 158861.5 64 114818.0 65 81785.5 66 57769.5 67 41915.0 68 30843.5 69 23243.0 70 17766.0 71 14014.5 72 11496.5 73 9071.5 74 7136.0 75 6285.0 76 4916.0 77 2769.5 78 1438.0 79 911.0 80 595.5 81 438.0 82 329.0 83 220.5 84 151.0 85 134.0 86 90.0 87 43.5 88 28.0 89 27.5 90 18.5 91 8.0 92 9.5 93 9.0 94 4.5 95 3.5 96 4.0 97 3.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10523263929902177 2 0.0 3 0.0 4 0.0 5 0.00954451940915517 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.449592984866251E-5 20-21 0.010713379772061667 22-23 0.0 24-25 8.396783028710836E-4 26-27 0.005016945834764336 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.7603318718471356E-6 40-41 0.007432121162938606 42-43 0.03912689651554628 44-45 0.015168779739706768 46-47 0.004847953975067012 48-49 0.0016318276452022948 50-51 0.0 52-53 0.0013836208512718485 54-55 5.809095177095547E-5 56-57 7.991906698185995E-4 58-59 2.4644646205859896E-5 60-61 2.781324357518474E-4 62-63 2.675704445207646E-4 64-65 5.985128364280261E-5 66-67 2.4996712580229325E-4 68-69 5.034549153482808E-4 70-71 4.013556667811469E-4 72-73 0.0011987860047278994 74-75 0.001649430963920766 76-77 0.002466224952457837 78-79 0.004575102534930706 80-81 0.004321614745384718 82-83 0.007476129459734785 84-85 0.002298993424632359 86-87 0.0026985887595416588 88-89 0.005728119910990579 90-91 0.0057545248890682865 92-93 0.0055855330293709616 94-95 0.002578886192256054 96-97 0.005610177675576821 98-99 0.007907410768337333 100 0.2979995412575142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8403735E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.809264540899136 #Duplication Level Percentage of deduplicated Percentage of total 1 68.34783637802155 28.57572771926793 2 17.043188961266875 14.251263918042776 3 5.787766743445393 7.259468126331803 4 2.710442837714558 4.532864864999732 5 1.4687349953219004 3.07033649799448 6 0.9459367503638701 2.372935190095291 7 0.6500988642462501 1.9026108775106683 8 0.4808540106829343 1.6083322030556113 9 0.36502121683376826 1.3735141755879532 >10 1.9482771320933543 14.303639934239461 >50 0.11039372254922854 3.2563912390966454 >100 0.13658771360214836 10.787960029703248 >500 0.004340473391516072 1.0658523587170545 >1k 4.7837397415275427E-4 0.36862576482122095 >5k 8.36529848810461E-6 0.019540363630285103 >10k+ 3.3461193942921195E-5 5.250936736905809 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1370213 4.824059230238559 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 85180 0.29989013768787803 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29302 0.10316248901772952 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0023130760796071363 1.7603318718471354E-5 0.0 0.0 0.0 2 0.0023553240445314673 1.7603318718471354E-5 0.0 3.5206637436942713E-6 0.0 3 0.0024327786468927412 1.7603318718471354E-5 0.0 3.5206637436942713E-6 0.0 4 0.002471505948073378 2.112398246216563E-5 0.0 7.041327487388543E-6 1.0561991231082814E-5 5 0.002506712585510321 2.46446462058599E-5 0.0 1.4082654974777085E-5 1.0561991231082814E-5 6 0.0025278365679724866 2.816530994955417E-5 0.0 1.4082654974777085E-5 1.7603318718471354E-5 7 0.0025524812141783466 2.816530994955417E-5 0.0 1.7603318718471354E-5 2.46446462058599E-5 8 0.002570084532896818 3.168597369324844E-5 0.0 2.112398246216563E-5 2.46446462058599E-5 9 0.0026158531615648436 3.168597369324844E-5 0.0 2.112398246216563E-5 2.46446462058599E-5 10-11 0.0026669027858484103 4.224796492433126E-5 0.0 2.112398246216563E-5 2.46446462058599E-5 12-13 0.002763721038800003 4.752896053987267E-5 0.0 2.112398246216563E-5 2.8165309949554167E-5 14-15 0.0028464566367768186 5.809095177095547E-5 0.0 2.2884314334012764E-5 3.520663743694271E-5 16-17 0.002962638540318729 6.865294300203829E-5 0.0 2.816530994955417E-5 3.8727301180636986E-5 18-19 0.0031703577011966914 7.92149342331211E-5 0.0 3.168597369324844E-5 4.224796492433126E-5 20-21 0.0033147049146881563 8.977692546420392E-5 0.0 4.400829679617839E-5 5.457028802726121E-5 22-23 0.0034977794293602586 9.153725733605106E-5 0.0 5.633061989910834E-5 5.985128364280261E-5 24-25 0.004145581558200004 1.0033891669528674E-4 0.0 7.041327487388542E-5 6.337194738649688E-5 26-27 0.006205169848261153 1.091405760545224E-4 0.0 7.217360674573256E-5 6.689261113019115E-5 28-29 0.012334645426032879 1.2322323102929947E-4 0.0 7.39339386175797E-5 7.041327487388542E-5 30-31 0.02792062381936742 1.3026455851668803E-4 0.0 8.625626172050965E-5 7.041327487388542E-5 32-33 0.05975446538985102 1.3730588600407658E-4 0.0 9.329758920789818E-5 7.39339386175797E-5 34-35 0.11536158888963019 1.443472134914651E-4 0.0 1.0033891669528674E-4 7.745460236127397E-5 36-37 0.20098413113627486 1.4962820910700652E-4 0.0 1.03859580438981E-4 7.92149342331211E-5 38-39 0.32136794685628495 1.584298684662422E-4 0.0 1.0561991231082814E-4 8.097526610496824E-5 40-41 0.4804702620975727 1.6547119595363075E-4 0.0 1.1266123979821668E-4 8.097526610496824E-5 42-43 0.6797996812743112 1.7603318718471356E-4 0.0 1.1266123979821668E-4 8.801659359235678E-5 44-45 0.9196378574859961 1.8659517841579636E-4 0.0 1.1442157167006382E-4 1.0561991231082814E-4 46-47 1.2006607581714164 1.9363650590318492E-4 0.0 1.1618190354191095E-4 1.1090090792636954E-4 48-49 1.5247044094729092 2.024381652624206E-4 0.0 1.2146289915745236E-4 1.1442157167006382E-4 50-51 1.8920856711274063 2.0947949274980915E-4 0.0 1.2498356290114663E-4 1.1618190354191095E-4 52-53 2.2981150190283075 2.182811521090448E-4 0.0 1.3202489038853515E-4 1.1618190354191095E-4 54-55 2.7442570492929894 2.2532247959643336E-4 0.0 1.4082654974777083E-4 1.1618190354191095E-4 56-57 3.2281652395362794 2.3588447082751617E-4 0.0 1.4610754536331226E-4 1.1618190354191095E-4 58-59 3.7469596868158357 2.4996712580229325E-4 0.0 1.5666953659439507E-4 1.1618190354191095E-4 60-61 4.296387429329276 2.534877895459875E-4 0.0 1.584298684662422E-4 1.1618190354191095E-4 62-63 4.873290431698507 2.5876878516152893E-4 0.0 1.7251252344101928E-4 1.1618190354191095E-4 64-65 5.480494026577842 2.622894489052232E-4 0.0 1.7603318718471356E-4 1.1618190354191095E-4 66-67 6.114213500442812 2.8165309949554167E-4 0.0 1.81314182800255E-4 1.2674389477299378E-4 68-69 6.772519529561869 2.886944269829302E-4 0.0 1.918761740313378E-4 1.3026455851668803E-4 70-71 7.457286867378533 2.974960863421659E-4 0.0 2.0067783339057345E-4 1.3026455851668803E-4 72-73 8.16204101326815 3.0805807757324877E-4 0.0 2.130001564935034E-4 1.3026455851668803E-4 74-75 8.883923188270838 3.168597369324844E-4 0.0 2.1652082023719766E-4 1.3026455851668803E-4 76-77 9.630839042823066 3.291820600354144E-4 0.0 2.182811521090448E-4 1.3026455851668803E-4 78-79 10.39296240441618 3.5030604249758E-4 0.0 2.182811521090448E-4 1.337852222603823E-4 80-81 11.173118605704495 3.7671102057528703E-4 0.0 2.2004148398089196E-4 1.337852222603823E-4 82-83 11.968714677840785 3.837523480626756E-4 0.0 2.2708281146828052E-4 1.337852222603823E-4 84-85 12.778396925615592 3.855126799345227E-4 0.0 2.3588447082751617E-4 1.3730588600407658E-4 86-87 13.6033923003436 3.943143392937584E-4 0.0 2.4996712580229325E-4 1.4082654974777083E-4 88 14.229526504172778 3.943143392937584E-4 0.0 2.534877895459875E-4 1.4082654974777083E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 171895 0.0 85.60463 8 AGTGGTA 174880 0.0 84.45335 5 TGGTATC 174435 0.0 84.422775 7 GTATCAA 175420 0.0 83.72906 9 GTGGTAT 176500 0.0 83.645386 6 GCAGTGG 183735 0.0 80.5571 3 AAGCAGT 181615 0.0 79.82865 1 CAGTGGT 187065 0.0 78.856766 4 AGCAGTG 192185 0.0 76.88555 2 CAACGCA 168100 0.0 44.47309 12-13 ATCAACG 169270 0.0 44.186516 10-11 ACGCAGA 170605 0.0 43.912994 14-15 TCAACGC 167795 0.0 43.85237 12-13 AGAGTAC 170650 0.0 43.628124 18-19 AACGCAG 169165 0.0 43.558826 14-15 GAGTACT 167130 0.0 43.49759 20-21 CGCAGAG 171855 0.0 42.95633 16-17 TATCAAC 174830 0.0 42.038063 10-11 AGTACTT 177080 0.0 41.762203 20-21 GTACTTT 176575 0.0 41.176163 22-23 >>END_MODULE