FastQCFastQC Report
Thu 26 May 2016
SRR1660751_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660751_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31357777
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13374084.264996208117687No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1436320.4580426731142326No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318410.10154099890435472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1665100.086.545958
GTGGTAT1688350.085.348586
TGGTATC1689950.085.031527
GTATCAA1698450.084.752559
AGTGGTA1706200.084.458975
GCAGTGG1803850.080.395363
CAGTGGT1809600.079.534374
AAGCAGT1827350.079.255131
AGCAGTG1890200.076.7458952
CAACGCA1624350.045.16537512-13
ATCAACG1638900.044.83748210-11
TCAACGC1618250.044.65929812-13
ACGCAGA1648550.044.4863714-15
AACGCAG1638700.044.18026414-15
CGCAGAG1659850.043.52440316-17
AGAGTAC1669900.043.40397318-19
GAGTACT1644200.043.02581420-21
TATCAAC1690250.042.57768210-11
AGTACTT1736650.041.40691420-21
GTACTTT1739000.040.64540522-23
Produced by FastQC (version 0.11.5)