##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660751_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31357777 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.764974092391817 33.0 31.0 34.0 30.0 34.0 2 31.922446096864583 34.0 31.0 34.0 30.0 34.0 3 31.96680877601751 34.0 31.0 34.0 30.0 34.0 4 35.39189318171374 37.0 35.0 37.0 33.0 37.0 5 35.38646881760783 37.0 35.0 37.0 33.0 37.0 6 35.39534961295247 37.0 35.0 37.0 33.0 37.0 7 35.391293330518934 37.0 35.0 37.0 33.0 37.0 8 35.384286169265124 37.0 35.0 37.0 33.0 37.0 9 37.0071471265326 39.0 37.0 39.0 33.0 39.0 10-11 36.96393561316543 39.0 37.0 39.0 33.0 39.0 12-13 36.973367420145884 39.0 37.0 39.0 33.0 39.0 14-15 38.26576427914517 40.0 38.0 41.0 33.0 41.0 16-17 38.23459223528505 40.0 38.0 41.0 33.0 41.0 18-19 38.1853952370412 40.0 38.0 41.0 33.0 41.0 20-21 38.15286805885506 40.0 38.0 41.0 33.0 41.0 22-23 38.04067943017772 40.0 38.0 41.0 32.5 41.0 24-25 37.95984487356996 40.0 38.0 41.0 33.0 41.0 26-27 37.918252894648745 40.0 38.0 41.0 32.0 41.0 28-29 37.8263338469433 40.0 38.0 41.0 32.0 41.0 30-31 37.709018611236374 40.0 37.5 41.0 32.0 41.0 32-33 37.583160215725755 40.0 37.0 41.0 31.5 41.0 34-35 37.45246799541945 40.0 37.0 41.0 31.0 41.0 36-37 37.26421461572356 40.0 37.0 41.0 30.5 41.0 38-39 36.87221726527362 39.5 35.0 41.0 30.0 41.0 40-41 36.861578389309926 39.0 35.0 41.0 30.0 41.0 42-43 36.89091846338469 40.0 35.0 41.0 30.0 41.0 44-45 36.83582471423277 40.0 35.0 41.0 30.0 41.0 46-47 36.958776701549986 40.0 35.0 41.0 30.0 41.0 48-49 36.90479144934285 40.0 35.0 41.0 30.0 41.0 50-51 35.7986802922924 38.5 34.0 40.0 28.5 40.5 52-53 35.862495609940716 38.5 35.0 40.0 28.0 40.5 54-55 36.2034276855786 39.0 35.0 41.0 28.0 41.0 56-57 35.93749166275403 39.0 35.0 41.0 26.5 41.0 58-59 35.66612618936604 39.0 35.0 41.0 26.0 41.0 60-61 35.44994278452838 39.0 35.0 41.0 26.0 41.0 62-63 35.20105207075106 38.5 34.5 41.0 25.5 41.0 64-65 34.93154176713483 38.0 34.0 40.0 25.0 41.0 66-67 34.653148770718026 37.0 34.0 40.0 25.0 41.0 68-69 34.36207244537775 37.0 34.0 40.0 24.0 41.0 70-71 34.04535458620042 36.0 33.5 39.0 24.0 41.0 72-73 33.710300621756446 35.5 33.0 39.0 24.0 41.0 74-75 33.36133717323138 35.0 33.0 38.5 23.5 40.5 76-77 32.984130475830604 35.0 33.0 37.0 22.5 39.5 78-79 32.62902523351703 35.0 32.5 37.0 22.0 39.0 80-81 32.29318328273079 35.0 32.0 36.5 20.5 39.0 82-83 31.977502646313226 35.0 32.0 36.0 20.0 38.0 84-85 31.70467008869921 35.0 32.0 35.5 20.0 37.0 86-87 31.44509378008524 35.0 31.5 35.0 20.0 37.0 88-89 31.245257277006594 35.0 31.0 35.0 19.0 36.0 90-91 31.067873577262827 35.0 31.0 35.0 19.0 36.0 92-93 30.900482677710222 35.0 31.0 35.0 18.0 36.0 94-95 30.734754603299844 35.0 31.0 35.0 18.0 35.5 96-97 30.6183874896489 34.0 31.0 35.0 18.0 35.0 98-99 30.514967802086225 34.0 30.5 35.0 17.0 35.0 100 30.394467917799147 34.0 30.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6924149287556851 1101 2 0.6648160211839489 1101 3 0.6520177079024627 1101 4 0.5561860368999874 1101 5 0.5629332400935283 1101 6 0.5972659182009181 1101 7 0.529386101607912 1101 8 0.5926250279103797 1101 9 0.7638733948812302 1101 10-11 0.7010995856312192 1101 12-13 0.6665526008931764 1101 14-15 0.823768639954082 1101 16-17 0.8432279694313678 1101 18-19 0.82989715222066 1101 20-21 0.7531085182281885 1101 22-23 0.7449458116552847 1101 24-25 0.7051931545180423 1101 26-27 0.7092072796305331 1101 28-29 0.646047094626482 1101 30-31 0.6967908908354659 1101 32-33 0.7167769130667452 1101 34-35 0.712488535197437 1101 36-37 0.7271331271547652 1101 38-39 0.7584683735933453 1101 40-41 0.7317282450635076 1101 42-43 0.6279640408175311 1101 44-45 0.589746018565009 1101 46-47 0.6658537512640237 1101 48-49 0.6901818996230062 1101 50-51 0.7265617978843082 1101 52-53 0.7671097980154684 1101 54-55 0.8074408823534256 1101 56-57 0.7316853261469873 1101 58-59 0.7395363305672902 1101 60-61 0.7321127643911964 1101 62-63 0.6935741735714913 1101 64-65 0.6194028045499707 1101 66-67 0.5953690169368215 1101 68-69 0.6381136208155453 1101 70-71 0.6124507980211007 1101 72-73 0.5733344842688197 1101 74-75 0.5305450714695681 1101 76-77 0.4847133466640088 1101 78-79 0.4538746762343351 1101 80-81 0.44893764583815 1101 82-83 0.44434410132075897 1101 84-85 0.38502886138262227 1101 86-87 0.32632717972901304 1101 88-89 0.37583065028336904 1101 90-91 0.36987950757639254 1101 92-93 0.33794762206672857 1101 94-95 0.2820332489927324 1101 96-97 0.29587186628434026 1101 98-99 0.270271838573688 1101 100 0.24020976942303562 1102 1 0.6250560712540825 1102 2 0.6193644082334622 1102 3 0.6049029306404883 1102 4 0.5521208101331325 1102 5 0.5117209349609197 1102 6 0.5513743774430253 1102 7 0.5289916772924244 1102 8 0.5780431525717304 1102 9 0.6966099240399188 1102 10-11 0.6232025375687797 1102 12-13 0.6218285112035744 1102 14-15 0.7938509592596574 1102 16-17 0.7974814668465484 1102 18-19 0.7899799514690713 1102 20-21 0.7717297937063918 1102 22-23 0.7056481272250679 1102 24-25 0.74151430827213 1102 26-27 0.7478852339034603 1102 28-29 0.7479733642386535 1102 30-31 0.7466393979855255 1102 32-33 0.7161511796639886 1102 34-35 0.6592237952795372 1102 36-37 0.7220969031518152 1102 38-39 0.7616651295281898 1102 40-41 0.7064337939908114 1102 42-43 0.6824071829748206 1102 44-45 0.6365993437947495 1102 46-47 0.7050102148883397 1102 48-49 0.744595575982423 1102 50-51 0.673093820783599 1102 52-53 0.7207442444004073 1102 54-55 0.7760312605210657 1102 56-57 0.731767544078501 1102 58-59 0.7338157405401304 1102 60-61 0.7377800835346093 1102 62-63 0.6872088543839538 1102 64-65 0.6494935200833751 1102 66-67 0.6149425453487112 1102 68-69 0.6354002053399057 1102 70-71 0.6075827401300131 1102 72-73 0.5784796873727558 1102 74-75 0.5162570997440667 1102 76-77 0.5103870931608157 1102 78-79 0.4747690691278166 1102 80-81 0.422644826531517 1102 82-83 0.4544515609987343 1102 84-85 0.36445616285210747 1102 86-87 0.37651730362198066 1102 88-89 0.3836640025649416 1102 90-91 0.3174404752662987 1102 92-93 0.28150393597421797 1102 94-95 0.27236896822014955 1102 96-97 0.24928206412731768 1102 98-99 0.2335172592081527 1102 100 0.2233624965418315 1103 1 0.5915868191564293 1103 2 0.5771980559833629 1103 3 0.564270232950836 1103 4 0.5127261187794616 1103 5 0.5018733466405081 1103 6 0.49942668425215686 1103 7 0.4781340089859043 1103 8 0.5269494152267811 1103 9 0.6430722038293837 1103 10-11 0.6124161387657026 1103 12-13 0.5959335366015281 1103 14-15 0.7550798601398085 1103 16-17 0.7301217351087246 1103 18-19 0.7148333729604204 1103 20-21 0.6600263834198543 1103 22-23 0.6634289314137121 1103 24-25 0.6810082695485065 1103 26-27 0.7238458452584382 1103 28-29 0.6922546539691865 1103 30-31 0.6675802044308696 1103 32-33 0.6557374279906654 1103 34-35 0.6431127015257019 1103 36-37 0.6762314529135125 1103 38-39 0.6725227468867132 1103 40-41 0.6603283524676087 1103 42-43 0.6440410042925535 1103 44-45 0.624409125405414 1103 46-47 0.6787533184589449 1103 48-49 0.6514752225052192 1103 50-51 0.5798686005964768 1103 52-53 0.6672455218839488 1103 54-55 0.7316847835787428 1103 56-57 0.7047287120403283 1103 58-59 0.7318789412296027 1103 60-61 0.6764647396390089 1103 62-63 0.6477217261329429 1103 64-65 0.6580759010825332 1103 66-67 0.5990339174329407 1103 68-69 0.6144984228118773 1103 70-71 0.5871891488784442 1103 72-73 0.5644039248554549 1103 74-75 0.5293739797667101 1103 76-77 0.5164077077689626 1103 78-79 0.4963340634489981 1103 80-81 0.44457756423782513 1103 82-83 0.4113379618772015 1103 84-85 0.4235144992816515 1103 86-87 0.40828643607425974 1103 88-89 0.43496188778105704 1103 90-91 0.39272290136192467 1103 92-93 0.33017595772481556 1103 94-95 0.3414023678533056 1103 96-97 0.3553247099976069 1103 98-99 0.31098536182910763 1103 100 0.32272923542728194 1104 1 0.5308101978073729 1104 2 0.5188452590716501 1104 3 0.4912317252379488 1104 4 0.4491391199653805 1104 5 0.4533347655107036 1104 6 0.4564706911534131 1104 7 0.4118801132716712 1104 8 0.4482982965357465 1104 9 0.5553251659153702 1104 10-11 0.5281974855203444 1104 12-13 0.5594039196246072 1104 14-15 0.681330049399179 1104 16-17 0.6607942652714272 1104 18-19 0.686345014765358 1104 20-21 0.6174920335141039 1104 22-23 0.5971949463784867 1104 24-25 0.6260873398076967 1104 26-27 0.635247966217932 1104 28-29 0.612420541089314 1104 30-31 0.6130280708690137 1104 32-33 0.6241447225017893 1104 34-35 0.6381643966722592 1104 36-37 0.6556730567133044 1104 38-39 0.6324953538993867 1104 40-41 0.5850023953425421 1104 42-43 0.5558998457760538 1104 44-45 0.5690671370160132 1104 46-47 0.616362858695851 1104 48-49 0.6275437215974691 1104 50-51 0.6023172733745312 1104 52-53 0.6222950481257641 1104 54-55 0.7061764864038551 1104 56-57 0.7038184375661913 1104 58-59 0.706210650402241 1104 60-61 0.7141879892794947 1104 62-63 0.6554709834021963 1104 64-65 0.6292078410468278 1104 66-67 0.6450535672347897 1104 68-69 0.6687258098739619 1104 70-71 0.6695288955762422 1104 72-73 0.6372018977982847 1104 74-75 0.5990909661901256 1104 76-77 0.6016414124469094 1104 78-79 0.5546600914941138 1104 80-81 0.5564995103811654 1104 82-83 0.5260166275765883 1104 84-85 0.4869349262769376 1104 86-87 0.5208662245180022 1104 88-89 0.5381322650235631 1104 90-91 0.47250522780830906 1104 92-93 0.45300755468825926 1104 94-95 0.4636026031943814 1104 96-97 0.48590609043694144 1104 98-99 0.42965184309369064 1104 100 0.37666757170088516 1105 1 0.42409750454301687 1105 2 0.42272405710477656 1105 3 0.3960791891426503 1105 4 0.38276325156172675 1105 5 0.3882055300760783 1105 6 0.395727205472852 1105 7 0.3530124725620567 1105 8 0.3915392617121469 1105 9 0.4696611972028464 1105 10-11 0.4545774527535187 1105 12-13 0.4615227071027306 1105 14-15 0.5513367263675804 1105 16-17 0.5343406565642823 1105 18-19 0.5481497401194204 1105 20-21 0.5144776084915677 1105 22-23 0.49541252105981215 1105 24-25 0.48996541862450016 1105 26-27 0.4983498260821193 1105 28-29 0.509876145310578 1105 30-31 0.5297775237180176 1105 32-33 0.5296652987450514 1105 34-35 0.5308095909705273 1105 36-37 0.5082412207245 1105 38-39 0.5251175300282682 1105 40-41 0.4984573189870929 1105 42-43 0.500105342277223 1105 44-45 0.49258503835901735 1105 46-47 0.5174471084211802 1105 48-49 0.5276057381761419 1105 50-51 0.4803785767979889 1105 52-53 0.506844210430863 1105 54-55 0.652490156936544 1105 56-57 0.634607650035683 1105 58-59 0.6620769602640095 1105 60-61 0.6689271421281688 1105 62-63 0.6100961006874854 1105 64-65 0.573748166569878 1105 66-67 0.5372507281865282 1105 68-69 0.5350682247068903 1105 70-71 0.5697399365912759 1105 72-73 0.5409452942938557 1105 74-75 0.5110533563175181 1105 76-77 0.5119595095423009 1105 78-79 0.514812534507989 1105 80-81 0.5517900624113494 1105 82-83 0.5271871258966385 1105 84-85 0.49098507475803643 1105 86-87 0.46371084575942234 1105 88-89 0.46012560959377424 1105 90-91 0.3839522807693676 1105 92-93 0.42091809597512153 1105 94-95 0.4191558488515099 1105 96-97 0.39262334685558287 1105 98-99 0.40820437884263683 1105 100 0.41702113822915265 1106 1 0.3355478461524122 1106 2 0.3050213195629645 1106 3 0.26635771049830126 1106 4 0.30072375126113116 1106 5 0.2622382247512647 1106 6 0.28600964460662226 1106 7 0.26436543112409794 1106 8 0.28430530881448846 1106 9 0.3456870553426441 1106 10-11 0.33913337034125846 1106 12-13 0.3462477550967691 1106 14-15 0.3970510502795719 1106 16-17 0.3809690677233135 1106 18-19 0.37971090735394597 1106 20-21 0.3964497104433704 1106 22-23 0.3159243386894772 1106 24-25 0.37263400653538525 1106 26-27 0.3803563486926649 1106 28-29 0.37241577926545943 1106 30-31 0.36600744256070783 1106 32-33 0.3175235072428748 1106 34-35 0.3062231343148625 1106 36-37 0.3199600957426796 1106 38-39 0.38219702069096684 1106 40-41 0.3462182047497322 1106 42-43 0.31155710976686635 1106 44-45 0.24315361853737016 1106 46-47 0.3401151716161763 1106 48-49 0.34967570165184014 1106 50-51 0.331218582353749 1106 52-53 0.34896505124384447 1106 54-55 0.34963303669646706 1106 56-57 0.3971678142536561 1106 58-59 0.4207370726059878 1106 60-61 0.45666303804524233 1106 62-63 0.45419049395998456 1106 64-65 0.36069684892861176 1106 66-67 0.42117733651675593 1106 68-69 0.3985640809968629 1106 70-71 0.42662925443372757 1106 72-73 0.3951579248034278 1106 74-75 0.37592242022713407 1106 76-77 0.362103857928723 1106 78-79 0.2974287860296414 1106 80-81 0.3013580410511949 1106 82-83 0.34329863988467224 1106 84-85 0.3268944263687743 1106 86-87 0.33775927336708733 1106 88-89 0.31023180952665896 1106 90-91 0.3183801543609839 1106 92-93 0.36842421005865944 1106 94-95 0.3470478884566077 1106 96-97 0.2746551251878451 1106 98-99 0.2575825384936259 1106 100 0.31949714636154525 1107 1 0.429697854612602 1107 2 0.40951995852394063 1107 3 0.35008038663378116 1107 4 0.40280692687437636 1107 5 0.4143701196010241 1107 6 0.4566487188102215 1107 7 0.412648346633965 1107 8 0.42223620791198613 1107 9 0.523839808647125 1107 10-11 0.4984583508403304 1107 12-13 0.4752918693728816 1107 14-15 0.5847072197167762 1107 16-17 0.6300675829378761 1107 18-19 0.608955066937142 1107 20-21 0.5633237287964263 1107 22-23 0.5437228748627732 1107 24-25 0.5912699751816888 1107 26-27 0.5709399083187066 1107 28-29 0.5649350343410262 1107 30-31 0.5792589776656456 1107 32-33 0.5665597872577735 1107 34-35 0.5520551346216394 1107 36-37 0.5616854033686494 1107 38-39 0.5975548959315589 1107 40-41 0.5719316374847239 1107 42-43 0.6132326422447392 1107 44-45 0.5613233646846183 1107 46-47 0.5651255575442846 1107 48-49 0.565206590376313 1107 50-51 0.5195450282524732 1107 52-53 0.5851289336046435 1107 54-55 0.6201520978615775 1107 56-57 0.5989792634503956 1107 58-59 0.6229986670985923 1107 60-61 0.5992417271591179 1107 62-63 0.5761611038287455 1107 64-65 0.5558583904357164 1107 66-67 0.5091334739043987 1107 68-69 0.5756268965741498 1107 70-71 0.5965664463819493 1107 72-73 0.5556171851712648 1107 74-75 0.5446524094213103 1107 76-77 0.562152837037047 1107 78-79 0.5605699834622158 1107 80-81 0.5375954917237848 1107 82-83 0.5288000255963183 1107 84-85 0.5280593211058004 1107 86-87 0.5164525522994765 1107 88-89 0.5489016149567476 1107 90-91 0.545959636282344 1107 92-93 0.5465239414722518 1107 94-95 0.5525698389827802 1107 96-97 0.5867237143372428 1107 98-99 0.5873164960679453 1107 100 0.5555017377690206 1108 1 0.2137213069751347 1108 2 0.17303301864415133 1108 3 0.21007431275765853 1108 4 0.20364204772950956 1108 5 0.20845370565295696 1108 6 0.22720595909046182 1108 7 0.22632395671887195 1108 8 0.2302157149187707 1108 9 0.24506017262167745 1108 10-11 0.2336656464310991 1108 12-13 0.22945034479138116 1108 14-15 0.2811739381830165 1108 16-17 0.2811161469888077 1108 18-19 0.27826587507777134 1108 20-21 0.24247829064514548 1108 22-23 0.2115338025629896 1108 24-25 0.2670091608785157 1108 26-27 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-1.6958849044171025 2316 82-83 -1.9308095382983055 2316 84-85 -1.6399159451260346 2316 86-87 -1.6031189302153237 2316 88-89 -1.7103187722718758 2316 90-91 -1.6040453589989383 2316 92-93 -1.5060210179993128 2316 94-95 -1.5110034688373943 2316 96-97 -1.3937135063300552 2316 98-99 -1.4420082141698778 2316 100 -1.6091195960168747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 29.0 4 71.0 5 165.0 6 1348.0 7 16347.0 8 22197.0 9 28829.0 10 32308.0 11 33758.0 12 38209.0 13 45602.0 14 57698.0 15 74741.0 16 92580.0 17 109768.0 18 121639.0 19 135554.0 20 153118.0 21 183956.0 22 236884.0 23 323929.0 24 441848.0 25 521125.0 26 516787.0 27 471172.0 28 450920.0 29 481049.0 30 554296.0 31 664869.0 32 821875.0 33 1050677.0 34 1420588.0 35 2086451.0 36 3385658.0 37 6135944.0 38 7946842.0 39 2691434.0 40 7512.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.672832338406156 34.2316993194529 28.111589879324566 19.983878462816374 2 21.0738862833129 32.46672677138138 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31.072781066552267 20.577640480518422 46-47 20.8459056276148 27.60376186782465 31.010403752985383 20.539928751575165 48-49 20.93627336563564 27.592290945194442 30.894252807343776 20.57718288182614 50-51 20.970981403591605 27.64660136428347 30.824839982451106 20.557577249673823 52-53 21.089270110222653 27.781544616441995 30.630678206245022 20.498507067090326 54-55 21.43313291989772 27.986579055561737 29.976424635175654 20.60386338936489 56-57 21.834016030489167 28.362762448328795 29.104075239399634 20.699146281782404 58-59 22.026067861323877 28.760865390583177 28.557682520880196 20.655384227212746 60-61 22.177534996251428 28.95837539894795 28.223225337339642 20.640864267460984 62-63 22.26898825035525 29.077431329551896 28.089662707564354 20.563917712528497 64-65 22.415645057293542 29.126134865685938 28.02756499270636 20.430655084314157 66-67 22.546296990819283 28.980173502599282 28.096683699443886 20.376845807137553 68-69 22.68167993063438 28.78067589999418 28.238762586278277 20.298881583093166 70-71 22.80915542024924 28.688224226984328 28.331285600720424 20.171334752046004 72-73 22.974803934573522 28.640588847564192 28.297197066642184 20.0874101512201 74-75 23.154943538544927 28.66986057711014 28.179248188064925 19.995947696280012 76-77 23.426621224657726 28.763330350360633 27.908929223940792 19.90111920104085 78-79 23.68888877757958 28.806229959240003 27.609653488259724 19.89522777492069 80-81 23.920942887338605 28.862026785248123 27.36886830603088 19.848162021382393 82-83 24.13096595406812 28.951044890637718 27.16486114318051 19.753128012113656 84-85 24.32578400900595 28.957146545211117 26.99937916340159 19.717690282381344 86-87 24.532734378234306 28.92129612900699 26.85405710158793 19.691912391170774 88-89 24.714985959695937 28.91734183602179 26.735884814208916 19.631787390073352 90-91 24.911760001370755 28.869516861937118 26.5929649414897 19.625758195202426 92-93 25.061842007130263 28.858168075263794 26.51624330316459 19.563746614441353 94-95 25.234264972237074 28.868161304717233 26.428008032183488 19.469565690862208 96-97 25.36993062610212 28.847688841753733 26.31665131172001 19.465729220424137 98-99 25.494534023952653 28.840617842269655 26.2270717921535 19.437776341624193 100 25.54839122196273 28.895318169966405 26.145133400013453 19.41115720805741 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 87285.0 1 53005.5 2 15663.5 3 12072.5 4 11365.5 5 11431.5 6 11812.5 7 12020.5 8 12237.5 9 12477.5 10 13157.5 11 15276.0 12 91968.0 13 114144.0 14 61166.5 15 65431.5 16 73647.0 17 82250.5 18 91433.0 19 101131.5 20 112953.5 21 131029.5 22 157749.5 23 187960.0 24 220184.0 25 257347.5 26 296566.5 27 342159.5 28 400084.5 29 457903.5 30 508482.5 31 564361.5 32 622727.0 33 672908.5 34 719862.5 35 774485.0 36 832621.0 37 877468.5 38 917723.0 39 952193.0 40 973771.5 41 1000537.0 42 1038848.0 43 1051117.0 44 1045445.5 45 1055971.5 46 1065213.5 47 1069191.0 48 1067688.5 49 1056272.5 50 1039866.5 51 1020028.5 52 989210.5 53 941959.0 54 883163.0 55 818489.5 56 750225.0 57 677017.0 58 596356.5 59 508546.0 60 417665.5 61 336056.5 62 263884.0 63 198708.0 64 146873.5 65 107109.0 66 77029.0 67 57118.0 68 43108.5 69 31656.0 70 23945.5 71 18869.0 72 15337.0 73 12507.5 74 10325.5 75 9123.5 76 7235.0 77 4668.5 78 3039.0 79 2300.0 80 1824.5 81 1429.5 82 1144.5 83 931.5 84 771.0 85 664.0 86 563.5 87 477.0 88 409.0 89 346.5 90 289.5 91 252.0 92 218.5 93 183.0 94 162.0 95 136.0 96 113.0 97 93.0 98 75.5 99 58.0 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046597690901367145 2 0.015045709394514797 3 0.005379845644032738 4 1.5945007836493002E-4 5 5.485082695753593E-4 6 0.0 7 3.1890015672986004E-6 8 0.0 9 0.0 10-11 4.7835023509479005E-6 12-13 2.7106513322038102E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.03862837598468794 22-23 0.0754326430728811 24-25 0.031320778893223204 26-27 0.035602013497321575 28-29 0.04021968776676995 30-31 0.033733258578884595 32-33 0.034350330382156874 34-35 0.03537399988525972 36-37 0.032956736697247385 38-39 0.043274751268242005 40-41 0.0638868628984765 42-43 0.0720236003974389 44-45 0.07492399732289697 46-47 0.047991284586276634 48-49 0.03045496496770163 50-51 0.028042485282040244 52-53 0.028425165470116077 54-55 0.029222415861940722 56-57 0.029343597921498068 58-59 0.03708968272846637 60-61 0.05692048897471271 62-63 0.07999610431568539 64-65 0.10815817715649932 66-67 0.08925058686398593 68-69 0.05294380402029136 70-71 0.04647491434102615 72-73 0.050639750387918125 74-75 0.0481905971842328 76-77 0.05102243057599396 78-79 0.04364945895239959 80-81 0.04224629826278821 82-83 0.04142353585842517 84-85 0.0490835176230764 86-87 0.052712601406662216 88-89 0.05853731276933311 90-91 0.05548224926786104 92-93 0.05107026559950344 94-95 0.0529709105336134 96-97 0.05475834591208426 98-99 0.04965434890362285 100 0.05134611423507476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.1357777E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.99555096293848 #Duplication Level Percentage of deduplicated Percentage of total 1 70.509200804847 31.02091137365709 2 15.74899823894863 13.857717092737856 3 5.499401722356528 7.25847626224825 4 2.4659214828540175 4.33958297078035 5 1.3760022212817338 3.0268987925758535 6 0.8647232463785983 2.2826385392932336 7 0.5798473928046005 1.7857493864603267 8 0.4291985790057921 1.5106262364696081 9 0.3401730431232824 1.3469490409453417 >10 1.9124687740516704 15.656338401903108 >50 0.16469157456836095 5.019743915027297 >100 0.10860491802853559 7.606683091807278 >500 3.198805798466782E-4 0.08846113802146298 >1k 4.0498730570992704E-4 0.33166272504614797 >5k 1.4377955265946428E-5 0.034607689337147626 >10k+ 2.875591032013865E-5 4.8329533436896925 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1337408 4.264996208117687 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 143632 0.4580426731142326 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31841 0.10154099890435472 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.006633123259981088 6.378003134597201E-6 0.0 0.0 9.567004701895801E-6 2 0.006636312261548387 2.87010141056874E-5 0.0 6.378003134597201E-6 1.2756006269194402E-5 3 0.006639501263115685 4.14570203748818E-5 0.0 6.378003134597201E-6 1.2756006269194402E-5 4 0.006639501263115685 4.7835023509479E-5 0.0 9.567004701895801E-6 1.5945007836493E-5 5 0.0066426902646829844 5.102402507677761E-5 3.1890015672986004E-6 9.567004701895801E-6 2.5512012538388804E-5 6 0.006645879266250283 5.4213026644076205E-5 3.1890015672986004E-6 2.2323010971090203E-5 3.1890015672986E-5 7 0.006649068267817582 5.74020282113748E-5 3.1890015672986004E-6 2.87010141056874E-5 3.1890015672986E-5 8 0.006649068267817582 6.3780031345972E-5 3.1890015672986004E-6 2.87010141056874E-5 3.1890015672986E-5 9 0.006649068267817582 7.33470360478678E-5 3.1890015672986004E-6 2.87010141056874E-5 3.5079017240284606E-5 10-11 0.006655446270952178 8.929204388436081E-5 3.1890015672986004E-6 2.87010141056874E-5 3.8268018807583204E-5 12-13 0.006665013275654074 1.052370517208538E-4 3.1890015672986004E-6 2.87010141056874E-5 4.14570203748818E-5 14-15 0.00669849779211071 1.1002055407180171E-4 3.1890015672986004E-6 2.87010141056874E-5 4.14570203748818E-5 16-17 0.006744738314836539 1.148040564227496E-4 3.1890015672986004E-6 3.5079017240284606E-5 4.14570203748818E-5 18-19 0.006802140343047915 1.3234356504289191E-4 3.1890015672986004E-6 4.14570203748818E-5 4.783502350947901E-5 20-21 0.006862731372826588 1.642335807158779E-4 3.1890015672986004E-6 4.14570203748818E-5 5.102402507677761E-5 22-23 0.007030153955109765 1.7380058541777372E-4 3.1890015672986004E-6 4.4646021942180405E-5 5.26185258604269E-5 24-25 0.007626497248194603 1.8177308933602023E-4 3.1890015672986004E-6 4.624052272582971E-5 5.4213026644076205E-5 26-27 0.010032598930721396 2.040961003071104E-4 3.1890015672986004E-6 5.102402507677761E-5 5.580752742772551E-5 28-29 0.016911275311384477 2.2163560892725273E-4 3.1890015672986004E-6 5.4213026644076205E-5 6.21855305623227E-5 30-31 0.03302689473172796 2.3439161519644712E-4 3.1890015672986004E-6 5.74020282113748E-5 6.856353369691991E-5 32-33 0.06605219496267226 2.55120125383888E-4 3.1890015672986004E-6 5.74020282113748E-5 7.334703604786782E-5 34-35 0.12085040339434776 2.7584863557132894E-4 3.1890015672986004E-6 5.74020282113748E-5 8.13195399661143E-5 36-37 0.20406739929300471 2.901991426241726E-4 3.1890015672986004E-6 6.696903291327061E-5 8.29140407497636E-5 38-39 0.3215422445283669 2.997661473260684E-4 3.1890015672986004E-6 7.33470360478678E-5 8.61030423170622E-5 40-41 0.47497148793423716 3.2368365908080795E-4 3.1890015672986004E-6 7.813053839881571E-5 8.929204388436081E-5 42-43 0.6685072095512383 3.396286669173009E-4 3.1890015672986004E-6 8.13195399661143E-5 8.929204388436081E-5 44-45 0.9011097948684308 3.4919567161919673E-4 3.1890015672986004E-6 8.45085415334129E-5 9.24810454516594E-5 46-47 1.173297456640501 3.842746888594813E-4 3.1890015672986004E-6 9.088654466801011E-5 9.726454780260731E-5 48-49 1.4881826603971322 3.9703069512867574E-4 3.1890015672986004E-6 9.5670047018958E-5 9.885904858625661E-5 50-51 1.847353528918839 4.0500319904692225E-4 3.1890015672986004E-6 9.885904858625661E-5 1.0045354936990591E-4 52-53 2.246560717617196 4.209482068834152E-4 3.1890015672986004E-6 1.0842605328815241E-4 1.1161505485545101E-4 54-55 2.6854040067955074 4.4008221628720685E-4 3.1890015672986004E-6 1.148040564227496E-4 1.1161505485545101E-4 56-57 3.163098902068217 4.655942288255956E-4 3.1890015672986004E-6 1.148040564227496E-4 1.1320955563910032E-4 58-59 3.6776235764416594 4.831337374457379E-4 3.1890015672986004E-6 1.148040564227496E-4 1.1799305799004821E-4 60-61 4.2248275443759935 5.118347515514253E-4 3.1890015672986004E-6 1.259655619082947E-4 1.243710611246454E-4 62-63 4.806444028223046 5.421302664407621E-4 3.1890015672986004E-6 1.27560062691944E-4 1.243710611246454E-4 64-65 5.4183305149468985 5.756147828973973E-4 3.1890015672986004E-6 1.27560062691944E-4 1.307490642592426E-4 66-67 6.057835349744339 5.883707891665917E-4 3.1890015672986004E-6 1.27560062691944E-4 1.307490642592426E-4 68-69 6.726538682891967 5.963432930848382E-4 3.1890015672986004E-6 1.27560062691944E-4 1.3712706739383982E-4 70-71 7.4225829209768275 6.043157970030847E-4 3.1890015672986004E-6 1.27560062691944E-4 1.482885728793849E-4 72-73 8.141047434580582 6.186663040559285E-4 3.1890015672986004E-6 1.27560062691944E-4 1.498830736630342E-4 74-75 8.882373900420301 6.282333087578243E-4 3.1890015672986004E-6 1.3553256661019052E-4 1.498830736630342E-4 76-77 9.645597645521875 6.409893150270187E-4 3.1890015672986004E-6 1.4031606896113842E-4 1.498830736630342E-4 78-79 10.433089692550592 6.505563197289144E-4 3.1890015672986004E-6 1.4669407209573563E-4 1.5466657601398212E-4 80-81 11.244366907769002 6.792573338346018E-4 3.1890015672986004E-6 1.5785557758128072E-4 1.5785557758128072E-4 82-83 12.074025528021327 6.952023416710949E-4 3.1890015672986004E-6 1.5945007836493002E-4 1.706115838504751E-4 84-85 12.918618242613308 6.999858440220428E-4 3.1890015672986004E-6 1.6104457914857933E-4 1.7539508620142302E-4 86-87 13.77843046718522 7.239033557767823E-4 3.1890015672986004E-6 1.642335807158779E-4 1.7539508620142302E-4 88 14.433947278852068 7.462263667478724E-4 3.1890015672986004E-6 1.690170830668258E-4 1.7539508620142302E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 166510 0.0 86.54595 8 GTGGTAT 168835 0.0 85.34858 6 TGGTATC 168995 0.0 85.03152 7 GTATCAA 169845 0.0 84.75255 9 AGTGGTA 170620 0.0 84.45897 5 GCAGTGG 180385 0.0 80.39536 3 CAGTGGT 180960 0.0 79.53437 4 AAGCAGT 182735 0.0 79.25513 1 AGCAGTG 189020 0.0 76.745895 2 CAACGCA 162435 0.0 45.165375 12-13 ATCAACG 163890 0.0 44.837482 10-11 TCAACGC 161825 0.0 44.659298 12-13 ACGCAGA 164855 0.0 44.48637 14-15 AACGCAG 163870 0.0 44.180264 14-15 CGCAGAG 165985 0.0 43.524403 16-17 AGAGTAC 166990 0.0 43.403973 18-19 GAGTACT 164420 0.0 43.025814 20-21 TATCAAC 169025 0.0 42.577682 10-11 AGTACTT 173665 0.0 41.406914 20-21 GTACTTT 173900 0.0 40.645405 22-23 >>END_MODULE