FastQCFastQC Report
Thu 26 May 2016
SRR1660751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31357777
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14595744.654583773588287No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1086260.34640848424937776No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415010.13234675404445922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1813950.086.8483668
GTGGTAT1847550.085.612296
TGGTATC1845550.085.473367
GTATCAA1842600.085.393429
AGTGGTA1864100.084.936855
GCAGTGG1947950.081.425483
CAGTGGT1956800.080.73544
AAGCAGT1938900.080.031561
AGCAGTG2036600.077.721932
CAACGCA1763800.045.2969912-13
ATCAACG1779950.044.96516810-11
TCAACGC1760100.044.7087212-13
ACGCAGA1795900.044.6222814-15
AACGCAG1779750.044.28638514-15
CGCAGAG1807850.043.72932416-17
AGAGTAC1828600.043.65293518-19
GAGTACT1801900.043.35481320-21
TATCAAC1841150.042.73799510-11
AGTACTT1903250.041.75366620-21
GTACTTT1904800.041.02152322-23
Produced by FastQC (version 0.11.5)