##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660751_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31357777 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.977356526261413 33.0 31.0 34.0 26.0 34.0 2 31.7690027899618 34.0 31.0 34.0 28.0 34.0 3 32.28273550768602 34.0 31.0 34.0 30.0 34.0 4 35.81544514459682 37.0 35.0 37.0 35.0 37.0 5 35.874236588901056 37.0 35.0 37.0 35.0 37.0 6 35.913200734860766 37.0 35.0 37.0 35.0 37.0 7 35.91728775926941 37.0 35.0 37.0 35.0 37.0 8 35.91650699601569 37.0 35.0 37.0 35.0 37.0 9 37.627382865819854 39.0 37.0 39.0 35.0 39.0 10-11 37.5772354334939 39.0 37.0 39.0 35.0 39.0 12-13 37.62697606083492 39.0 37.0 39.0 35.0 39.0 14-15 38.952147931277146 40.0 38.0 41.0 35.0 41.0 16-17 38.964641402992314 40.0 38.0 41.0 35.0 41.0 18-19 38.94604537815292 40.0 38.0 41.0 35.0 41.0 20-21 38.890042747609314 40.0 38.0 41.0 35.0 41.0 22-23 38.7981339844339 40.0 38.0 41.0 34.5 41.0 24-25 38.73555446867296 40.0 38.0 41.0 34.0 41.0 26-27 38.68369577027096 40.0 38.0 41.0 34.0 41.0 28-29 38.578433078977504 40.0 38.0 41.0 34.0 41.0 30-31 38.48375092086407 40.0 38.0 41.0 34.0 41.0 32-33 38.38174729350234 40.0 38.0 41.0 34.0 41.0 34-35 38.27659250207692 40.0 38.0 41.0 34.0 41.0 36-37 38.10254252079157 40.0 38.0 41.0 33.0 41.0 38-39 37.7283533045088 40.0 36.5 41.0 33.0 41.0 40-41 37.587457538842756 40.0 36.0 41.0 33.0 41.0 42-43 37.43400914548248 40.0 36.0 41.0 32.5 41.0 44-45 37.324681386056156 40.0 35.5 41.0 32.0 41.0 46-47 37.19719747672164 39.0 35.0 41.0 32.0 41.0 48-49 37.32718542197682 40.0 36.0 41.0 32.0 41.0 50-51 37.24754465216077 40.0 35.0 41.0 31.5 41.0 52-53 37.01991765870393 39.0 35.0 41.0 31.0 41.0 54-55 36.585902278723395 39.0 35.0 41.0 30.0 41.0 56-57 36.34537274437534 39.0 35.0 41.0 29.0 41.0 58-59 36.17437197477359 39.0 35.0 41.0 28.0 41.0 60-61 35.98500461305022 39.0 35.0 41.0 28.0 41.0 62-63 35.699011141638 38.5 35.0 41.0 28.0 41.0 64-65 35.457967460512265 38.0 35.0 40.0 28.0 41.0 66-67 35.19761005379941 37.0 34.5 40.0 27.5 41.0 68-69 34.911929327770906 37.0 34.0 40.0 27.0 41.0 70-71 34.58866087669416 36.0 34.0 39.0 26.5 41.0 72-73 34.27757369407914 36.0 34.0 39.0 26.0 41.0 74-75 33.9250928884404 35.0 34.0 38.5 26.0 40.0 76-77 31.821213410631756 34.0 31.0 36.0 23.0 39.0 78-79 33.094557818942334 35.0 33.0 37.0 25.5 39.0 80-81 33.07334818408843 35.0 33.0 36.5 26.0 39.0 82-83 32.84549215653903 35.0 33.0 36.0 25.5 38.0 84-85 32.60094604282695 35.0 33.0 36.0 25.0 37.0 86-87 32.38528529940117 35.0 33.0 35.0 25.0 37.0 88-89 32.1573088870426 35.0 33.0 35.0 24.5 36.5 90-91 31.988483574585022 35.0 33.0 35.0 24.0 36.0 92-93 31.854174930831356 35.0 33.0 35.0 24.0 36.0 94-95 31.74822284117908 35.0 33.0 35.0 23.5 36.0 96-97 31.637286676922283 35.0 33.0 35.0 23.0 35.0 98-99 31.514174266881227 35.0 32.5 35.0 22.0 35.0 100 31.321854671011916 35.0 32.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7543597097141195 1101 2 1.2007643030462454 1101 3 0.7669146101748225 1101 4 0.6069651250740122 1101 5 0.46211691872594685 1101 6 0.4238393517361345 1101 7 0.3823556555853216 1101 8 0.40522503952405486 1101 9 0.5078158250794615 1101 10-11 0.5176101676738085 1101 12-13 0.4668331151065743 1101 14-15 0.6263066003687427 1101 16-17 0.6243374717332699 1101 18-19 0.5635071211744958 1101 20-21 0.5966455603008569 1101 22-23 0.5818525360850586 1101 24-25 0.6257111086737694 1101 26-27 0.6104279049867358 1101 28-29 0.6516179158330573 1101 30-31 0.6190290411374448 1101 32-33 0.6040494215861401 1101 34-35 0.6246926741998564 1101 36-37 0.6053310447445526 1101 38-39 0.6883613092875294 1101 40-41 0.6842726414242009 1101 42-43 0.6458390504706557 1101 44-45 0.6072331106381554 1101 46-47 0.6065074406686861 1101 48-49 0.5838675146914767 1101 50-51 0.5773268406645116 1101 52-53 0.5755465643808577 1101 54-55 0.6611334556734718 1101 56-57 0.7207086718983717 1101 58-59 0.700534832178036 1101 60-61 0.6411076742961583 1101 62-63 0.6409349047437374 1101 64-65 0.6328922618797534 1101 66-67 0.5438841027468868 1101 68-69 0.5476336023878545 1101 70-71 0.5914775187249077 1101 72-73 0.5153131815121696 1101 74-75 0.5230117156422835 1101 76-77 0.45207165670759153 1101 78-79 0.5728217032335223 1101 80-81 0.5358235302651551 1101 82-83 0.49505309252815977 1101 84-85 0.4978998365414 1101 86-87 0.45789605927067356 1101 88-89 0.49188822172353497 1101 90-91 0.5006891788516938 1101 92-93 0.40108448329128166 1101 94-95 0.3374078577952595 1101 96-97 0.31510340854405783 1101 98-99 0.30809048907686076 1101 100 0.24590502310725526 1102 1 1.6775901392625734 1102 2 1.150962483405518 1102 3 0.7175911573282008 1102 4 0.5660413768645398 1102 5 0.43206707951274126 1102 6 0.421547070266449 1102 7 0.36487509970537957 1102 8 0.3794787472112233 1102 9 0.4740085521475663 1102 10-11 0.4612802898101478 1102 12-13 0.39797504010584106 1102 14-15 0.5936215937871481 1102 16-17 0.5607010323183559 1102 18-19 0.5212514622741509 1102 20-21 0.5477565329466074 1102 22-23 0.5454984139799137 1102 24-25 0.5684299421180441 1102 26-27 0.5673149739207446 1102 28-29 0.5877449402305928 1102 30-31 0.5667491205719486 1102 32-33 0.5269432476560496 1102 34-35 0.5303830480234524 1102 36-37 0.5716671547373195 1102 38-39 0.6317302554094724 1102 40-41 0.5575255791832561 1102 42-43 0.5472579587218576 1102 44-45 0.5464027330914405 1102 46-47 0.5889910910315947 1102 48-49 0.5617665823153857 1102 50-51 0.523090313505584 1102 52-53 0.47603589224585363 1102 54-55 0.5046686196244394 1102 56-57 0.5778349182895468 1102 58-59 0.5449209027160933 1102 60-61 0.5258774682458451 1102 62-63 0.5616285413470976 1102 64-65 0.5335160450197165 1102 66-67 0.5122741503162729 1102 68-69 0.489220651803393 1102 70-71 0.49224247911161 1102 72-73 0.4358882777769537 1102 74-75 0.42050359453021713 1102 76-77 0.3749447941521744 1102 78-79 0.4940684779245501 1102 80-81 0.46299986019687367 1102 82-83 0.42143426368927805 1102 84-85 0.40661166836404306 1102 86-87 0.39056842973332806 1102 88-89 0.3803044467695358 1102 90-91 0.34784134374042 1102 92-93 0.3118542561741684 1102 94-95 0.22116321611753875 1102 96-97 0.22354596352374756 1102 98-99 0.2111701302459288 1102 100 0.1529769809141044 1103 1 1.670530620090279 1103 2 1.1187235879520792 1103 3 0.6693766335979348 1103 4 0.5468259879923494 1103 5 0.40770458691799405 1103 6 0.3660469456311688 1103 7 0.3346219848160743 1103 8 0.3634532573293825 1103 9 0.44158631133931436 1103 10-11 0.4185318454660063 1103 12-13 0.37367535964718 1103 14-15 0.520933829255604 1103 16-17 0.486243517964688 1103 18-19 0.4031450202222189 1103 20-21 0.43852114848441204 1103 22-23 0.47856978095885694 1103 24-25 0.5410201487294017 1103 26-27 0.4932810059926567 1103 28-29 0.5043596137179946 1103 30-31 0.5131679784332519 1103 32-33 0.49723346692198334 1103 34-35 0.5085857420785658 1103 36-37 0.538071525386087 1103 38-39 0.5675480003417803 1103 40-41 0.5334935630129536 1103 42-43 0.5077971998050046 1103 44-45 0.5235744569835674 1103 46-47 0.5332738380246695 1103 48-49 0.5083177888351074 1103 50-51 0.42988678742474207 1103 52-53 0.454078489715549 1103 54-55 0.48621112714845083 1103 56-57 0.5273332491058156 1103 58-59 0.49924134897857897 1103 60-61 0.5007231351524908 1103 62-63 0.518285028154601 1103 64-65 0.4869559413315585 1103 66-67 0.4870083010502597 1103 68-69 0.5028991660519395 1103 70-71 0.4966307573862636 1103 72-73 0.4102712020166024 1103 74-75 0.3603453021032479 1103 76-77 0.24427972725257874 1103 78-79 0.38486592495043936 1103 80-81 0.4102816347575384 1103 82-83 0.3760604616133989 1103 84-85 0.3759543110950361 1103 86-87 0.3452940848889696 1103 88-89 0.36423712708441514 1103 90-91 0.36107126837559633 1103 92-93 0.33945100248529414 1103 94-95 0.29831268023078295 1103 96-97 0.2603743368823501 1103 98-99 0.2746869595303565 1103 100 0.2446307775924872 1104 1 1.5899293399394416 1104 2 1.0837010781156557 1104 3 0.6362442276595743 1104 4 0.5119932952403374 1104 5 0.37029042475456464 1104 6 0.33804820229195087 1104 7 0.2931099540615989 1104 8 0.31939356273122144 1104 9 0.39936868532397796 1104 10-11 0.3877431452128235 1104 12-13 0.3488401892669728 1104 14-15 0.5126645456538981 1104 16-17 0.4744534977752508 1104 18-19 0.40746956603074835 1104 20-21 0.3947741968076173 1104 22-23 0.38644637930042336 1104 24-25 0.4657285397132753 1104 26-27 0.4513831374314847 1104 28-29 0.45767341762127955 1104 30-31 0.42913812719520905 1104 32-33 0.42808874713232115 1104 34-35 0.3880660952215962 1104 36-37 0.4405125596972894 1104 38-39 0.4867895827554136 1104 40-41 0.4905624754726077 1104 42-43 0.4738988423546999 1104 44-45 0.43550629534495755 1104 46-47 0.46546086205560044 1104 48-49 0.47611083802395626 1104 50-51 0.45088875715035925 1104 52-53 0.41195021148671884 1104 54-55 0.446007986978735 1104 56-57 0.5173288697131753 1104 58-59 0.45789226143394757 1104 60-61 0.4587127120421357 1104 62-63 0.5357660326084428 1104 64-65 0.5192498772497487 1104 66-67 0.5077043043692271 1104 68-69 0.5054256066169245 1104 70-71 0.48272881678379775 1104 72-73 0.44163512662819926 1104 74-75 0.3979113326353314 1104 76-77 0.37182777962578584 1104 78-79 0.4416113729410185 1104 80-81 0.41202876212901174 1104 82-83 0.4008754097909417 1104 84-85 0.3722810877583598 1104 86-87 0.3857569821221247 1104 88-89 0.4159707298952853 1104 90-91 0.4221389239971174 1104 92-93 0.39654185437803946 1104 94-95 0.34960537671883074 1104 96-97 0.3235739111025424 1104 98-99 0.3669714644255464 1104 100 0.28317833051041674 1105 1 1.5913698550130597 1105 2 1.0472280922379475 1105 3 0.5959309935272259 1105 4 0.5015916965344758 1105 5 0.33783820122884123 1105 6 0.27984694229422047 1105 7 0.21614760235735275 1105 8 0.25603666789277213 1105 9 0.3178641042569339 1105 10-11 0.29082741755910035 1105 12-13 0.2798590119536968 1105 14-15 0.3977411700626803 1105 16-17 0.319530577308889 1105 18-19 0.3309330520836298 1105 20-21 0.3325234853571004 1105 22-23 0.3250362022757969 1105 24-25 0.3742948580064933 1105 26-27 0.3301296591898648 1105 28-29 0.34790302459147426 1105 30-31 0.3547166731400395 1105 32-33 0.3746704504855103 1105 34-35 0.354033940136695 1105 36-37 0.3837260393372972 1105 38-39 0.4353163273745153 1105 40-41 0.4098307299567807 1105 42-43 0.370236644786786 1105 44-45 0.3660889882835292 1105 46-47 0.41944443242726237 1105 48-49 0.37376342688342135 1105 50-51 0.3748980580407064 1105 52-53 0.3323411177350053 1105 54-55 0.40990053779172086 1105 56-57 0.4291311040569141 1105 58-59 0.4302155211929133 1105 60-61 0.38431700636428445 1105 62-63 0.40123029838057533 1105 64-65 0.386558647695054 1105 66-67 0.40869946814003555 1105 68-69 0.42888461393820876 1105 70-71 0.4120262468815383 1105 72-73 0.36980492294115663 1105 74-75 0.3051437649513389 1105 76-77 0.26248822260891913 1105 78-79 0.4138742607401369 1105 80-81 0.3897652644301246 1105 82-83 0.3473330274932138 1105 84-85 0.39286219506575293 1105 86-87 0.3424792470788205 1105 88-89 0.3864091563733538 1105 90-91 0.3882283446118109 1105 92-93 0.3237599911196263 1105 94-95 0.32104665105542907 1105 96-97 0.3343672359308343 1105 98-99 0.36097707723519434 1105 100 0.27993594205415917 1106 1 1.4187948085155178 1106 2 0.8775506243901781 1106 3 0.4738808652168487 1106 4 0.43970597166396885 1106 5 0.2528423309791137 1106 6 0.2386235360351776 1106 7 0.17660853812632382 1106 8 0.18517526659425698 1106 9 0.25218897471204826 1106 10-11 0.20369740464701636 1106 12-13 0.19145075193042516 1106 14-15 0.29131644667628365 1106 16-17 0.24264106445708222 1106 18-19 0.18497042346510284 1106 20-21 0.2166331200178746 1106 22-23 0.20611305046124784 1106 24-25 0.253328163468062 1106 26-27 0.2257141699487164 1106 28-29 0.23017922855247264 1106 30-31 0.2494148000425227 1106 32-33 0.22257345986935206 1106 34-35 0.22773702477505964 1106 36-37 0.2352776867981774 1106 38-39 0.308098961329172 1106 40-41 0.3236646432544603 1106 42-43 0.2665424428747514 1106 44-45 0.2733359273968574 1106 46-47 0.32381487551288757 1106 48-49 0.31266951135871324 1106 50-51 0.2880477357864919 1106 52-53 0.2590269495992956 1106 54-55 0.28948491053621694 1106 56-57 0.30135829156240135 1106 58-59 0.24997403477734537 1106 60-61 0.2800726790444017 1106 62-63 0.3163147543935594 1106 64-65 0.30485802540161444 1106 66-67 0.28766080761104007 1106 68-69 0.29818474119939964 1106 70-71 0.2787728714040725 1106 72-73 0.2541614138111612 1106 74-75 0.263211080019083 1106 76-77 0.19615978157274938 1106 78-79 0.3055318244153966 1106 80-81 0.3104580391802685 1106 82-83 0.2534431495265963 1106 84-85 0.2740152235547342 1106 86-87 0.2532235693852485 1106 88-89 0.28679146316464 1106 90-91 0.27725984699076633 1106 92-93 0.24503389675967568 1106 94-95 0.21992002371682418 1106 96-97 0.24603138217699438 1106 98-99 0.27308550404422505 1106 100 0.2547588875081068 1107 1 1.5706015465626884 1107 2 1.031451083763006 1107 3 0.5948941456993353 1107 4 0.4883452113751332 1107 5 0.3467451544894544 1107 6 0.3131839034329005 1107 7 0.25835002603813706 1107 8 0.28812498194172775 1107 9 0.34398069471536985 1107 10-11 0.3005390968339512 1107 12-13 0.2794820227376036 1107 14-15 0.4242512290710394 1107 16-17 0.38937647041488077 1107 18-19 0.34788613176404937 1107 20-21 0.34853636959837786 1107 22-23 0.364732253978687 1107 24-25 0.40972985161801034 1107 26-27 0.35353115779365396 1107 28-29 0.3451499116049632 1107 30-31 0.3837281613951973 1107 32-33 0.4089559440133641 1107 34-35 0.4245758704867413 1107 36-37 0.4563931505664627 1107 38-39 0.4575633831635315 1107 40-41 0.47421797281429434 1107 42-43 0.46135511021711295 1107 44-45 0.42048746333311726 1107 46-47 0.45277017714228407 1107 48-49 0.4590428639754691 1107 50-51 0.44582310858182694 1107 52-53 0.3920772313773 1107 54-55 0.4144467279132513 1107 56-57 0.42702059208334475 1107 58-59 0.4026210129750183 1107 60-61 0.3572372857026238 1107 62-63 0.4196097079687462 1107 64-65 0.4063218488080622 1107 66-67 0.39993661898348165 1107 68-69 0.36680631657677765 1107 70-71 0.4007268898936047 1107 72-73 0.39082016643186535 1107 74-75 0.37458989443395296 1107 76-77 0.3116665289743814 1107 78-79 0.4029092546295985 1107 80-81 0.40854531586242615 1107 82-83 0.39646943316127903 1107 84-85 0.3847590965474268 1107 86-87 0.33991701641309646 1107 88-89 0.37402293092628724 1107 90-91 0.4104601435203179 1107 92-93 0.4213417726200639 1107 94-95 0.41312880205802927 1107 96-97 0.3464530361196836 1107 98-99 0.40012972303821215 1107 100 0.3787308794082378 1108 1 1.4193000592201237 1108 2 0.8888796468389337 1108 3 0.44769875062651465 1108 4 0.3856770197638255 1108 5 0.2141752728988635 1108 6 0.16220327636036558 1108 7 0.12809680555469072 1108 8 0.12253232338495224 1108 9 0.15062672494239848 1108 10-11 0.11587204458656686 1108 12-13 0.11002196898814276 1108 14-15 0.15786883746890368 1108 16-17 0.11885955093790557 1108 18-19 0.093648259718357 1108 20-21 0.10453816854403897 1108 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46-47 -1.7485714112121045 2316 48-49 -1.7346854647545555 2316 50-51 -1.5489948690933986 2316 52-53 -1.3844561281967955 2316 54-55 -1.255076480897067 2316 56-57 -1.345749029539256 2316 58-59 -1.2311797085812586 2316 60-61 -1.182085771806321 2316 62-63 -2.235212429109403 2316 64-65 -1.5539166737830072 2316 66-67 -1.2562408148828226 2316 68-69 -1.214491939754602 2316 70-71 -1.1060084277831805 2316 72-73 -1.0466062336830504 2316 74-75 -1.1653172508500802 2316 76-77 -1.0418091575666928 2316 78-79 -1.3217169387683114 2316 80-81 -1.1590286153636669 2316 82-83 -1.0643828420931278 2316 84-85 -0.9563836334358271 2316 86-87 -1.1579351297572984 2316 88-89 -1.1140586515501845 2316 90-91 -1.0713561677101282 2316 92-93 -0.9680136791390375 2316 94-95 -0.9803366463907359 2316 96-97 -0.9318724821202657 2316 98-99 -0.9425785556557287 2316 100 -0.891025173968977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 53.0 7 843.0 8 1039.0 9 443.0 10 213.0 11 181.0 12 299.0 13 1350.0 14 5847.0 15 15371.0 16 25833.0 17 34278.0 18 43370.0 19 55580.0 20 74818.0 21 105985.0 22 160173.0 23 249291.0 24 389394.0 25 493159.0 26 498672.0 27 450393.0 28 436006.0 29 480518.0 30 565193.0 31 686886.0 32 844292.0 33 1066360.0 34 1426961.0 35 2086695.0 36 3478611.0 37 6547212.0 38 8517602.0 39 2608973.0 40 5883.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.29778486768135 34.01330728603584 28.111961792416135 19.576946053866674 2 21.27861295779991 32.636299441762084 27.30053217739255 18.784555423045454 3 24.961498386827614 26.942436002399024 27.052395965441043 21.043669645332322 4 20.44610496464721 27.468825357103597 26.711466185884287 25.37360349236491 5 20.73389509578889 32.18213490782458 26.64700147249759 20.436968523888936 6 25.941440300439666 26.851562213737278 26.715455626844975 20.491541858978078 7 20.538617900114538 27.051295759900327 31.954353779606254 20.45573256037888 8 25.60161710442676 26.98739135749323 26.847190092588512 20.563801445491496 9 25.58153277255591 26.770921931105 27.043119159881773 20.604426136457313 10-11 20.210431370820707 29.844939263392302 29.578166526281503 20.36646283950549 12-13 20.215505072314276 27.16093363378405 29.513404282452804 23.110157011448866 14-15 20.242986293320474 32.50408662578345 26.75999322273387 20.492933858162203 16-17 23.03753547325756 27.03683044879106 26.73723491304884 23.18839916490254 18-19 20.367706547823104 29.732743899036123 26.68824453285073 23.21130502029004 20-21 23.08418940792073 29.731895042870743 26.656907560688676 20.527007988519856 22-23 23.079741909000756 27.09936836402657 29.338941979209814 20.48194774776286 24-25 20.501648161506928 29.72055773997784 26.508487255630953 23.269306842884284 26-27 20.535230288023374 27.031621873457496 31.873115455791908 20.560032382727226 28-29 20.524490623171406 27.117972999170192 31.83563044025729 20.52190593740111 30-31 20.568275614690414 27.10871532762032 31.78724531397745 20.535763743711808 32-33 20.57193532200856 27.124260548256668 31.745482655634085 20.55832147410069 34-35 20.625955086038147 27.152986641878346 31.704686846902447 20.516371425181067 36-37 20.668197259907718 27.12636412735393 31.62318016430757 20.582258448430782 38-39 20.678041048636832 27.1374370702362 31.60585809383108 20.57866378729589 40-41 20.697145486898844 27.206728275337273 31.59439344407118 20.501732793692703 42-43 20.721640377498225 27.25026422521262 31.5035731377526 20.524522259536553 44-45 20.764313103233217 27.27460109806803 31.447362946461016 20.51372285223774 46-47 20.809111190939134 27.366150007003377 31.34250454836885 20.482234253688635 48-49 20.85800928607239 27.37837012218182 31.226158848009888 20.537461743735896 50-51 20.85507528164385 27.448646949686516 31.14120302596705 20.555074742702583 52-53 20.94587946081471 27.599443084254595 30.91062886969725 20.544048585233448 54-55 21.26983434514829 27.81096174935585 30.221881764224896 20.697322141270966 56-57 21.621817057309016 28.242906777112903 29.223166073451694 20.912110092126387 58-59 21.710954628181618 28.67863782018421 28.62876660645086 20.981640945183315 60-61 21.759105847144756 28.941937702599883 28.24667742490269 21.05227902535267 62-63 21.761344657228783 29.074294555677177 28.079841885094798 21.084518901999243 64-65 21.692912408409615 29.18795027838968 28.023086857928135 21.096050455272568 66-67 21.698862580094215 29.05109483464506 28.057854916915808 21.19218766834492 68-69 21.635198354210914 28.92811878714261 28.17960971346582 21.257073145180662 70-71 21.585771224222945 28.86857741007151 28.265048215685123 21.28060315002042 72-73 21.547433779586317 28.834302269958606 28.262604680234972 21.355659270220105 74-75 21.55907854911647 28.8817451253841 28.132483363385113 21.426692962114313 76-77 21.58505943175998 28.954817396752404 27.965211036440063 21.494912135047556 78-79 21.678350246786948 29.041204635774665 27.61437784995656 21.666067267481832 80-81 21.71737119097376 29.145902290685388 27.360152269601233 21.776574248739617 82-83 21.73210961365062 29.277085984217916 27.12042874147169 21.87037566065977 84-85 21.77727644769413 29.328074586912003 26.899891777057515 21.99475718833635 86-87 21.81389028726105 29.346652657414612 26.73828068412319 22.10117637120115 88-89 21.92464564319783 29.337623246202956 26.56638815676866 22.17134295383055 90-91 21.915079610861614 29.28306062335806 26.459230287774503 22.342629478005822 92-93 21.946157665929334 29.229584279768133 26.429502770780942 22.394755283521587 94-95 21.97976052122953 29.210131003241578 26.385460708626677 22.42464776690221 96-97 22.011660935902995 29.11385437318742 26.342425137954596 22.53205955295499 98-99 22.007946547859184 29.02299068231568 26.368160497131964 22.60090227269317 100 22.011068066958067 28.99736750445762 26.33720643279693 22.65435799578738 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 75618.0 1 46890.5 2 14017.5 3 9326.5 4 8564.0 5 8405.5 6 8526.0 7 8818.0 8 9146.0 9 9504.0 10 10254.0 11 12760.0 12 104412.0 13 127628.0 14 59744.0 15 62690.0 16 71833.0 17 81251.0 18 90713.5 19 100679.5 20 114283.0 21 134114.5 22 161084.0 23 193053.5 24 226351.0 25 263421.5 26 304426.0 27 351195.5 28 409322.5 29 467812.5 30 518665.0 31 573957.0 32 631981.5 33 681431.5 34 725536.0 35 779024.5 36 836421.0 37 879705.0 38 919485.5 39 953820.5 40 977507.5 41 1007167.0 42 1046554.0 43 1058098.5 44 1051375.5 45 1059712.0 46 1063690.5 47 1058897.0 48 1051786.0 49 1043833.0 50 1033116.5 51 1019728.5 52 994223.5 53 947704.0 54 889708.0 55 823776.5 56 752473.5 57 675690.0 58 590672.0 59 500335.0 60 409527.5 61 328830.5 62 255881.5 63 190296.5 64 139219.0 65 100356.0 66 71343.0 67 52215.5 68 38596.5 69 28121.5 70 20967.5 71 16133.0 72 12857.5 73 10075.5 74 8176.0 75 7188.5 76 5501.0 77 3154.5 78 1707.0 79 1106.0 80 762.5 81 520.0 82 339.5 83 223.0 84 161.0 85 120.5 86 78.5 87 56.5 88 44.0 89 26.5 90 16.5 91 10.5 92 10.5 93 12.5 94 12.0 95 8.0 96 5.0 97 2.0 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10757777887125097 2 0.0 3 0.0 4 0.0 5 0.009206647524791058 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.61030423170622E-5 20-21 0.011067429939309792 22-23 0.0 24-25 8.33923909848584E-4 26-27 0.0049573029363656745 28-29 0.0 30-31 0.0 32-33 1.5945007836493002E-6 34-35 0.0 36-37 3.1890015672986004E-6 38-39 0.0 40-41 0.007468641670613322 42-43 0.0394096813686761 44-45 0.015311991025384228 46-47 0.004722911321169227 48-49 0.0015753667742455086 50-51 0.0 52-53 0.0013728651747220474 54-55 5.580752742772551E-5 56-57 8.211679035793895E-4 58-59 2.5512012538388804E-5 60-61 3.0614415046066563E-4 62-63 2.4076961833104432E-4 64-65 7.49415368315171E-5 66-67 3.0773865124431493E-4 68-69 5.564807734936058E-4 70-71 4.4327121785450545E-4 72-73 0.0012022535908715723 74-75 0.001631174301673234 76-77 0.0024348026966324815 78-79 0.0043944441597374715 80-81 0.004109028519464247 82-83 0.007285274080493652 84-85 0.002383778671555704 86-87 0.002807915880006418 88-89 0.0055488627270995645 90-91 0.005693962298411651 92-93 0.005502622204373735 94-95 0.00268832832123272 96-97 0.005477110191835346 98-99 0.007655198262300289 100 0.3031018429654628 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.1357777E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.13565263642934 #Duplication Level Percentage of deduplicated Percentage of total 1 69.44519429676538 29.95563778454616 2 16.5849819179854 14.308080379913596 3 5.503609632586204 7.12205380073235 4 2.5015305992598886 4.31620619956294 5 1.3864206542589523 2.9902079875042635 6 0.8793867309318779 2.2759752335137584 7 0.6195549930942467 1.8707436279895164 8 0.4825900621842012 1.6653469830536431 9 0.34991345607045216 1.3584370764480753 >10 1.968504824547699 15.878591119172613 >50 0.16606790174286032 4.956932088302294 >100 0.11141105075146145 7.585419787003417 >500 3.6329361906371917E-4 0.10287563530898551 >1k 4.193360053141266E-4 0.3489342652792496 >5k 1.4642913573951308E-5 0.0358514114895986 >10k+ 3.660728360560161E-5 5.228706620179682 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1459574 4.654583773588287 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108626 0.34640848424937776 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41501 0.13234675404445922 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.006508752198856443 1.2756006269194402E-5 0.0 0.0 3.1890015672986004E-6 2 0.00651513020199104 1.9134009403791602E-5 0.0 3.1890015672986004E-6 3.1890015672986004E-6 3 0.006518319203558339 3.1890015672986E-5 0.0 3.1890015672986004E-6 3.1890015672986004E-6 4 0.0065215082051256375 4.4646021942180405E-5 0.0 3.1890015672986004E-6 3.1890015672986004E-6 5 0.006527886208260235 5.4213026644076205E-5 0.0 3.1890015672986004E-6 3.1890015672986004E-6 6 0.006531075209827534 6.696903291327061E-5 0.0 3.1890015672986004E-6 3.1890015672986004E-6 7 0.006534264211394832 7.33470360478678E-5 0.0 3.1890015672986004E-6 6.378003134597201E-6 8 0.006543831216096728 9.24810454516594E-5 0.0 3.1890015672986004E-6 6.378003134597201E-6 9 0.006543831216096728 9.5670047018958E-5 0.0 3.1890015672986004E-6 6.378003134597201E-6 10-11 0.006545425716880377 1.1320955563910032E-4 0.0 3.1890015672986004E-6 7.9725039182465E-6 12-13 0.006558181723149571 1.3712706739383982E-4 0.0 4.7835023509479005E-6 1.27560062691944E-5 14-15 0.006591666239606207 1.498830736630342E-4 0.0 6.378003134597201E-6 1.7539508620142303E-5 16-17 0.006631528759197439 1.674225822831765E-4 0.0 6.378003134597201E-6 2.39175117547395E-5 18-19 0.006688930787408814 2.088796026580583E-4 0.0 6.378003134597201E-6 3.1890015672986E-5 20-21 0.006757494321105734 2.375806167637457E-4 0.0 7.9725039182465E-6 4.30515211585311E-5 22-23 0.0069344839080908065 2.503366230329401E-4 0.0 1.4350507052843702E-5 5.4213026644076205E-5 24-25 0.0075690952199832275 2.614981285184852E-4 0.0 1.7539508620142303E-5 5.4213026644076205E-5 26-27 0.010050138439341539 2.8860464184052336E-4 0.0 1.9134009403791602E-5 6.0591029778673406E-5 28-29 0.017148855928148223 3.1092765281161354E-4 0.0 2.7106513322038102E-5 6.3780031345972E-5 30-31 0.033537134982495734 3.268726606481065E-4 0.0 3.5079017240284606E-5 7.49415368315171E-5 32-33 0.06704716345166942 3.380341661336516E-4 0.0 3.5079017240284606E-5 7.813053839881571E-5 34-35 0.12259957075401104 3.4919567161919673E-4 0.0 3.8268018807583204E-5 8.13195399661143E-5 36-37 0.20702679274745783 3.6035717710474185E-4 0.0 4.14570203748818E-5 8.29140407497636E-5 38-39 0.32618861981192093 3.9224719277772784E-4 0.0 4.14570203748818E-5 8.61030423170622E-5 40-41 0.48123309251162794 4.0181419747962365E-4 0.0 4.4646021942180405E-5 9.407554623530872E-5 42-43 0.6765530605055327 4.161647045324673E-4 0.0 4.4646021942180405E-5 1.0204805015355521E-4 44-45 0.9105651845154712 4.2892071080166173E-4 0.0 4.783502350947901E-5 1.1002055407180171E-4 46-47 1.185010659397189 4.4805472020545336E-4 0.0 5.102402507677761E-5 1.1161505485545101E-4 48-49 1.502850473105922 4.5602722412369987E-4 0.0 5.26185258604269E-5 1.163985572063989E-4 50-51 1.865634480403378 4.7197223196019283E-4 0.0 5.4213026644076205E-5 1.2118205955734681E-4 52-53 2.268652525974657 4.927007421476337E-4 0.0 5.4213026644076205E-5 1.2118205955734681E-4 54-55 2.7119970908652102 5.245907578206198E-4 0.0 5.4213026644076205E-5 1.227765603409961E-4 56-57 3.1953700034284958 5.612642758445537E-4 0.0 5.8996528995024104E-5 1.243710611246454E-4 58-59 3.7156237191175894 5.89965289950241E-4 0.0 6.696903291327061E-5 1.291545634755933E-4 60-61 4.267923711556467 6.250443071905257E-4 0.0 7.015803448056921E-5 1.3872156817748912E-4 62-63 4.855589731376686 6.473673181616159E-4 0.0 7.49415368315171E-5 1.4031606896113842E-4 64-65 5.473380654502391 6.665013275654074E-4 0.0 7.653603761516641E-5 1.4031606896113842E-4 66-67 6.1197195196585525 6.760683322673032E-4 0.0 8.29140407497636E-5 1.4031606896113842E-4 68-69 6.796038188548888 6.792573338346018E-4 0.0 8.61030423170622E-5 1.4031606896113842E-4 70-71 7.5010275760300225 6.872298377528484E-4 0.0 8.61030423170622E-5 1.4031606896113842E-4 72-73 8.228477101549641 7.111473495075879E-4 0.0 8.61030423170622E-5 1.450995713120863E-4 74-75 8.980673279231496 7.366593620459767E-4 0.0 8.61030423170622E-5 1.498830736630342E-4 76-77 9.755497017534118 7.589823730170668E-4 0.0 1.0842605328815241E-4 1.5307207523033281E-4 78-79 10.553893855422213 7.797108832045078E-4 0.0 1.211820595573468E-4 1.674225822831765E-4 80-81 11.37644100217946 7.924668894737022E-4 0.0 1.27560062691944E-4 1.7698958698507232E-4 82-83 12.217712371639099 8.084118973101952E-4 0.0 1.3393806582654122E-4 1.7858408776872162E-4 84-85 13.075051525495574 8.147899004447924E-4 0.0 1.3393806582654122E-4 1.7858408776872162E-4 86-87 13.948424979232424 8.275459067139867E-4 0.0 1.3393806582654122E-4 1.8177308933602023E-4 88 14.614613784644236 8.355184106322332E-4 0.0 1.3393806582654122E-4 1.881510924706174E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 181395 0.0 86.848366 8 GTGGTAT 184755 0.0 85.61229 6 TGGTATC 184555 0.0 85.47336 7 GTATCAA 184260 0.0 85.39342 9 AGTGGTA 186410 0.0 84.93685 5 GCAGTGG 194795 0.0 81.42548 3 CAGTGGT 195680 0.0 80.7354 4 AAGCAGT 193890 0.0 80.03156 1 AGCAGTG 203660 0.0 77.72193 2 CAACGCA 176380 0.0 45.29699 12-13 ATCAACG 177995 0.0 44.965168 10-11 TCAACGC 176010 0.0 44.70872 12-13 ACGCAGA 179590 0.0 44.62228 14-15 AACGCAG 177975 0.0 44.286385 14-15 CGCAGAG 180785 0.0 43.729324 16-17 AGAGTAC 182860 0.0 43.652935 18-19 GAGTACT 180190 0.0 43.354813 20-21 TATCAAC 184115 0.0 42.737995 10-11 AGTACTT 190325 0.0 41.753666 20-21 GTACTTT 190480 0.0 41.021523 22-23 >>END_MODULE