FastQCFastQC Report
Thu 26 May 2016
SRR1660750_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660750_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29120312
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15927855.469670105182939No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT295140.10135193606442129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2026300.085.744268
GTGGTAT2045050.084.884636
AGTGGTA2056550.084.426535
TGGTATC2051800.084.3718647
GTATCAA2060500.084.207119
AAGCAGT2117450.082.523771
GCAGTGG2122000.082.510333
AGCAGTG2183900.080.162042
CAGTGGT2175550.079.823614
CAACGCA2003450.044.59198812-13
ATCAACG2014850.044.41778610-11
ACGCAGA2024450.044.1086614-15
AGAGTAC2020650.043.61688218-19
TCAACGC1999950.043.6096112-13
AACGCAG2014900.043.35690714-15
GAGTACT1979950.043.12543520-21
CGCAGAG2034600.042.87133816-17
TATCAAC2056350.042.16454310-11
AGTACTT2104550.041.57884220-21
GTACTTT2081000.041.03586622-23
Produced by FastQC (version 0.11.5)